实验二十二心血管活动的神经体液调节

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3 II Exp. 1 Instruments for Experiments

4 Exp.2 Preparation of Sciatic Nerve Gastrocnemius Sample ExcitabilityExcitation Stimulus

5 Exp.3 Excitatory Transmission at Neuromuscular Junction

6 Acetylcholine 1% 1% 20 Exp.4 Action Potential of Nerve Trunks Action Potential Threshold

7 1-2 / 1 2 / 50 S 1 S 2 r 1 r r 1 r 2

8 1 Explain the Mechaism of Monophasic and Biphasic Action Potential 2 Exp.5 Relationship Between Intensity of Stimuli and Action Potential of Nerve Trunks Threshold Intensity Threshold Stimulus All or None Y Y

9 1Tell Whether the Value of the Action Potential Recorded During Excitation of Nerve Trunks is All or None.Why? 2 Exp.6 Measurement of Velocity of Conduction in Nerve Trunks Conduction 4-1 S 1 S 2 r 1 r 2 r 3 1 / 1 / t 1 S 1 r 1 r 2 t 2 S 2 V=s 2 -s 1 /t 2 -t 1 /

10 1 Tell Whether the Velocity Caculated in the Experiment Can Stand for That of All Fibers in Nerve Trunks?Why? 2 Exp.7 Measurement of Refractory Period of Sciatic Nerve Absolute Refractory Period Relative Refractory Period Subnormal Period Y A Y

11 MPA-2000 Exp.8 Twitch Contraction Summation and Tetanization of Skeletal Muscles MPA-V 0.5ms 50 / MPA-2000 Exp.9 Measurement of the Hematocrit

12 40-50% 38%-45% Wintrobe s hematocrit tube, K 2 C 2 O. 4 H 2 O,[(NH 2 ) 2 C 2 O. 4 H 2 O] /

13 Exp.10 Test of Erythrocyte Osmotic Fragility osmotic pressure 0.9%NaCl NaCl %NaCl NaCl % NaCl %NaCl %NaCl 75%4% 10 1%NaCl % 0.25%

14 %NaCl(ml) ml NaCl % Exp.11 Test of Erythrocyte Sedimentation Rate anticoagulant Westergreen %

15 / 22 Exp.12 Determination of Blood Types and Cross-Matching A B B A A B A or B agglutiogen. A B 75% ABO A B A B 75% % 10

16 % / Exp.13 Measurement of Bleeding Time capillary % 75% 2-3

17 2 Exp. 14 Measurement of Blood Coagulating Time 2-8 Blood Clotting Factors 75% 75% 2-3 Exp.15 Blood Coagulation intrinsic pathway and extrinsic pathway %1 /

18 [ ] Describe the basic process of blood coagulation Exp.16 Pacemaker of the Heart in Frog Cardac conduction system Automatic Rhythmicity SA mode Pacemaker

19 Trace the spread of activation of heart muscle,naming (in proper order)of each of the structure through which the impulse passes. Exp.17 Extrasystole, Compensatory Pause Effective refractory period Period of Supernormal excitability Extrasystole Compensatory puase

20 < / 10 DC /

21 Cardiac muscles can not in a titanic fashion,why? MPA B DC 1k 1ms 2ms 10V Exp.18 Effect of various factors on Cardiac Contraction of frog heart in vitro Cardiac innervation neurotransmitter receptor Sympathetic nerve β-contractility M %NaCl 2%CaCl 2 1%KCl 3% 0.5%HCl 2.5%NaHCO 3 ( 0.5%NaOH) 1/ 1/

22 Na %NaCl Ca %CaCl 2 K %KCl

23 1/ 1-2 1/ 1-2 1/ %NaHCO 3 1 ( 0.5%NaOH),. 3% ( 0.5%HCl)1 2.5%NaHCO 3 (0.5%NaOH) What effects of changes in Na +,K +,Ca 2+ concentration would be observed to affect the cardiac contraction? Why? MPA K DC :1ms B Exp.19 Factors controlling cardiac output Cardiac output Stroke volume heart rate preload

24 after load end diastolic volume epinephrine Ach

25 3 =

26 What is the underlying mechanism associated with a change in the vigor of cardiac contraction that is depedent on end-0diastolec volume? Exp.20 Microculation in the mesentery the frog arteriole venule capillary

27 20% 20%2-3 / True of false:strong vasoconstriction of arteiloes leading to a capillary bed will result in a loss of plasma by filtration Exp. 21 Afferent discharges of the depressor nerve in the rabbit baroreceptor reflex aortic archreceptorthe fibers from the aortic arch form a separte distinct branch of the vagus only in the rabbit 20% 5%

28 /

29 An increase in arterial blood pressure causes a increase in baroreceptor nerve activity, what will happen in sympathetic nerve activity to the arterioles MPA-2000 : :5000 :10Kc 0.01s Exp.22 Neural and hormonal control mechanism involved with cardivovascular regulation. xymapthetic and parasympathetic nerve. Vagal cardiac nrerve, atrial contractility

30 30 5% 3 1

31 What cardiovascular changes occur fallowing the stiulation of buffer nerver?

32 EXP.23 Measuring the pulmonary volumes and capacities gas exchange spirometer 75% % Tidal volumeinspiratory reserve volume Expiratory reserve volume Vital capacity Timed vial capacity

33 % 95% 98% Maximal Respiratory Volume / / 1 25 / 1 67 / 75% 75% EXP.24Measuring Lung compliance compliance distensibility elastic resistance trans mural pressure surface tension

34 T T

35 /10 EXP.25 Regulation of respiration respiratory centers O 2 CO 2 Y % 3% CO 2 20%1 /

36 5 / / Y CO 2 CO 2 CO 2 CO 2 CO 2 CO 2 N 2 N % 2 EXP.26 Intrapleural pressure and pneumothorax

37 % 20%5 / 5 / 2 5 /

38 Exp. 27 Characteristic of smooth muscle of the gut smooh muscle extendencyautorhythm NNaOH 1NHC1 38 2/

39 DC 2 5 / / N HC1 2 1N HC1 2 1N NAOH 2 38

40 peristalsis % %0 5 / ,

41 Exp. 29 Factors of Influence Urinary Formation filtration of glomerulus reabsorption and secretion of renal tubule and collecting duct ureter 5 20 [ 1 ] 20% 0 1% 20% % 4 0 1% % %

42 20% 2 20% / 2 [ 1] [ 2] 1 2 Exp.30 Measuremrnt of the visual field retinapathway for visual information

43 what is the trichromtic theory of color vision Exp.31 Measruement of the blind spot ther are no visual receptors overlying the otic disk this spot is blind + 50

44 15 = = 500 Exp 32 Audidtoy pathways K 256 / 512 / + -

45 Exp 33 Effect of damdging the labyrinth on one side Exp 34 Micrphonic effect

46 [ ] 20% % 6 / y [ ] Exp 35 Analysis of Reflex Arc reflexsensor afferernt nerve fiber neural center efferent nerve

47 fiber effcetor 0 5% 0 5% ] Exp 36 Reflex in spinal amimals 0 5%

48 0 5% Exp.37 Decerebrate Rigidity facilitation inhibition 2% 2%

49 Exp.38 Stereotaxic coordination in the brain 3% AP 0 H- 10 L 0 H H % 35 /

50 6 1/ AAP 0 1 LL LR 4 L 0 4 H Exp.30 Location of the Motor Cortex cerebral cortex 20% 20% 1 /

51 5-10 Which area of the ma motor cortex comtrbutes to control of hind limb of the rabits? Exp.40 Spontaneous Carebral Discharge and Evoked Potential in Cortex electroencephalogram % /

52 / / What is called evoked potential of cortex? Exp.41 Effect of thyroxine on energy metabolism in mouse thyroid hormone metabolism

53 oxygenconsumption /10 / L L VO =4 825 VO 2 =//// = / // / // 3

54 Exp.42 urine pregnancy test placenta [Human choroinic gonadotropin(hcg)] HCG hcg agglutination test hcg hcg hcg hcg hcg hcg hcg

55 1 1 2 hcg hcg hcg hcg hcg Exp.43 Design for physiology experiments

56 1 g/1000ml Kreds Krebs-Henseleit Locke Tyrode Ringer NAC1 KC1 MgC1 2 NaH 2 PO 4 NaHCO3 Glucose Pyruvic acid Fumaric acid Glutamic acid (

57 0.43 ) ml Krdbs-Henseleit Locke Tyrode Ringer % NaC1 KC1 CaC1 2 NaHCO 3 NaH2PO4 KH2PO4 MgC12 MgSO4-7H2O Glucose ( ) Krebs Krbs-Henseleit Locke Tyrode Ringer Na+ K+ Ca++ Mg C H2PO HCO SO4-0.5 Glucose

58 O2+5%CO2 O2+5%CO2 O2 O2 % / / CaC1 2 NaHCO3AK NaH2PO4 CaCO3 Ca3 PO4 2 2 PH Krebs Locde Tyrode PH Ringer PH7 2

59 1 2 1Ether /0 1 / 2 1 Sodium Pentobarbital 3-5% Sodium Pentothal 2 5-5% Chioralose 1% / 3-4

60 4Urethane 10-25% / /

62 T

63 20 (Radioimmunoassay,RIA) 60 Yalow Berson *Ag Ag Ag *Ag+Ab * Ag- Ab(- + Ag Ag- Ab - *Ag Ag Ab * Ag Ag Ab

64 *Ag Ab * Ag Ag Ab * Ag- Ab Ag Ag * Ag- Ab * Ag- Ab Ag * Ag- Ab B * Ag F B/T Ag Ag Ab * Ag- Ab Ag * Ag- Ab T * Ag B/T Ag 1 2 PH * Ag- Ab 1 B 2 3

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为提高教学质量，深化教学内容改革计划，推动教学改革，使全校教学工作进一步科学化、规范化，培养适应现代化建设和医学教育全球化需要的高素质、创新型的卫生科技人才，学校决定统一组织修编各专业课程的教学大纲南方医科大学本科专业教学大纲机能实验学 Functional Experiment 基础医学专业临床医学专业医学实验技术专业等执笔人 : 李明, 金春华审定人 : 金春华学院负责人 : 南方医科大学教务处二六年十二月一课程简介机能实验学是一

实验二十二 心血管活动的神经体液调节

实验二十二心血管活动的神经体液调节