52 33 thurianspermatophoreextracts(p>0.05)thevisceralindextestosteronelevelsinserumthickness ofspermatogenicepitheliumandnumberofspermatogeniccelswere

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忍袁影干
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1 CHINESE JOURNAL OF MARINE DRUGS Vol.33 No.2 April2014 * 1 1* ( ; ; ) : 本文旨在探讨海参精囊提取物对环磷酰胺导致的雄激素部分缺乏生殖系统受损小鼠的保护作用昆明种小白鼠随机分组除正常组用生理盐水外其他各组均腹腔注射环磷酰胺 (CP28mg/kg) 每天 1 次连续 5d 复制生殖系统受损雄激素部分缺乏模型 ; 同时治疗组每天灌胃不同剂量的药物 1 次连续 4 周记录小鼠的体重并观察其精神状态计算脏器指数检测血清中睾酮含量观察睾丸组织切片中生精上皮的厚度和生精细胞的数量并与模型组进行比较相比于模型组小鼠虽海参精囊提取物高低剂量间无明显差异 (P>0.05) 但治疗组小鼠的脏器指数血清睾酮含量生精上皮厚度和生精细胞数量均有明显的改善 (P<0.01) 甚至接近或高于正常水平海参精囊提取物能显著提高环磷酰胺导致的生殖系统受损小鼠的血清睾酮含量并且对损伤的生殖系统具有一定的保护作用 : 海参精囊 ; 环磷酰胺 ; 雄激素部分缺乏 ; 生精细胞 :R965 :A : (2014) DOI: /j.cnki.cjmd Effectsofholothurianspermatophoreextractsonmicegenital systeminjuredbycyclophosphamide GUO Xi-chun 1 GAO Hua 1* LIU Kun 1 LIU Xiao-ping 2 LIUZhan-tao 1 ZHANGJie 1 LIU Chun-bao 3 WU Yan-qiang 3 (1.Schoolof PharmaceuticalScienceQingdao UniversityQingdao266021China; 2.Schoolof MedicineQingdao UniversityQingdao266021China; 3.Dalian BangchuiIsland Seafood Co.LtdDalian116100China) Abstract:ObjectiveToinvestigatetheprotectiveefectsofholothurianspermatophoreextractsonpar- tialandrogendeficiencyofthe micegenitalsysteminjuredbycyclophosphamide(cp).methods Male Kunmingmicewereequalyalotedtogroupsandthey wereintraperitonealyinjected with CP (28 mg/kg)onceadayfor5daystoconstructinjuredgenitalsystemandpartialandrogendeficiencymodel exceptthattheregulargroupwasintraperitonealyinjectedwithnormalsaline.miceinthetreatment groupsoralyreceivedextractsonceadayfor4weeks.thechangesofbodyweightandspiritstatewere observed.visceralindextestosteroneconcentrationinserumthicknessofspermatogenicepithelium andspermatogeniccelsamount weredeterminedandcompared withthoseof modelgroup.results Comparedwiththatinmodelgroupthoughtherewasnoobviousdiferencebetweenthedosesofholo- * : ( ) : (1988-) : gxch3803@163.com * : Tel: gaohuaqy@126.com :

2 52 33 thurianspermatophoreextracts(p>0.05)thevisceralindextestosteronelevelsinserumthickness ofspermatogenicepitheliumandnumberofspermatogeniccelswerealsignificantlyimproved (P< 0.01)evenclosertoorhigherthanthenormallevel.Conclusion Holothurianspermatophoreextracts canimprovetestosteronelevelsinserumcontentofthemicewithdamagedgenitalsystemcausedbycp andhaveaprotectiveefectonthedamagedgenitalsystem. Keywords:holothurianspermatophore;cyclophosphamide;partialandrogendeficiency;spermatogenic cels (PAD- : ) AM) AR5120 ( ( ) (T) );DK-98-1 ( [1] 1 );SK3200H ( ); ( [2] ); B1 (TST) ( );LD4-2A ( );DFM-96 ( [3-4] ); ( ) 1.3 样品制备 : 1 3 [5] 30 25min 30 2h 4000r/min 5 min MPa 实验动物与原料 96 (25~35)g SPF 1 5 :SLXK : h; 1.4 动物分组及模型试剂与仪器设备 0.9% ( 28 mg kg -1 d -1 5d : ); (5 mg/ :110802); 5 ( : ( mg kg -1 d -1 ) ); (0.2g/ ( mg kg -1 d -1 )

3 2 : 53 (600mg kg -1 d -1 ) 1.5mg kg -1 d 指标检测珡 X±S SPSS17.0 ; 10% LSD 24h 1.6 观察指标及方法一般状态观察 ; = / 100% SNK 2 HE ( 1) 海参精囊提取物对小鼠脏器指数的影响 ( 见表 1) Fig.1 Thesurvivalcurveofmice (P<0.05); (P<0.01) (P<0.01) 2.3 海参精囊提取物对小鼠血清睾酮含量的影响 ( 见表 1) (P<0.01)

4 (n=10 X±s) 珡 Table1 Efectsofholothurianspermatophoreextractsonvisceralindexandtestosterone concentrationinserum(n=10 X±s) 珡 /Group (SI) /% (TI) /% /Serum testosteroneconcentration)/pg ml ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±59.60 : P<0.05 P<0.01 ; P<0.01 Note: P<0.05 P<0.01vscontrolgroup; P<0.01vsmodelgroup. 2.4 海参精囊提取物对睾丸组织形态的影响 ( 22) ( 23~7) 2 ( H.E. 100) Fig.2 Thehistologicalstructuresinthetestisofmice(parafinsectionH.E. 100) 1 ;2 ;3~4 ;5~6 ;7 ;8 1Controlgroup;2Modelgroup;3~4Lowandhighdosesofwaterextractsgroup;5 ~ 6Lowandhighdosesofalcoholextracts group;7freeze-driedpowdergroup;8positivedruggroup (P<0.01); ( 2) 0.01) (P<

5 2 : 55 2 (n=10 X±s) 珡 Table2 Efectsofholothurianspermatophoreextractsonthethicknessofspermatogenicepithelium andspermatogeniccelsamountofmice(n=10 X±s) 珡 /Group /Spermatogenic epitheliumthickness/mm /Spermatogenic celsamount/mm ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± : P<0.01 ; P<0.01 Note: P<0.01vscontrolgroup; P<0.01vsmodelgroup. 3 (cyclophosphamidecp) [6] ; DNA [7] [9] CP :(1)CP CP CP c-kit p53 Fas/FasL (2) [8] CP A CP CP (SCFm) Ser- toli A (3)CP PADAM TST Sertoli (4)CP 3β-HSD 17β-HSD TST

6 56 33 [2] TST CP CP [4] [10] [5] [6] CP CP PADAM [J] (15): [1]. [J] (8): [J] (3): [3]. [J] (16):. -1 [J]. : (3S): [J] (8): [J] (4): [7] EmersonREUlbrightT M.Intratubulargermcelneopla- siaofthetestisanditsassociatedcancers:theuseofnovelbi- omarkers.[j].pathology201042(4): [8] Yan WSouminenJToppariJ.Stem celfactorprotects germcelsfromapoptosisinvitro[j].j CelSci (6): [9]. [J] (10): [10].

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<4D F736F F D20BFB9CBA5C0CFCAB3C1C6CAB3C6D72020CFC2> 抗衰老食疗食谱第十一章老年营养保健第一节老年人注意保肾饮水注意 : 人体内许多有毒的代谢产物主要经肾脏排泄因此, 保持一定的饮水量是很重要的, 每日饮水 1500-2000 毫升为宜即使口不渴, 也应适当饮水, 夏日或汗多时, 应增