214 华北农学报 28 卷 Key words Summer corn Nutrition deficiency Chlorophyll fluorescence Photosynthesis rate ATP 36 57'N 'E 28 m 2 Peng

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佩暗案裘
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1 华北农学报 2013,28 ( 5 ) : P K Fo P N Fv /Fm Φ PSⅡ ETR K Φ PSⅡ ETR K NPK N P P % Φ PSⅡ ETR Φ PSⅡ ETR CO 2 N CO 2 P S143 A Effects of Long-term Nutrient Deficiency on Photosynthetic Characteristics of Summer Maize LI Fu-jie 1 2 WU Lan-fang 1 MA Jun-hua 1 KANG Hua-jing 1 1. Yucheng Comprehensive Experiment Station Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing China 2. Graduate University of Chinese Academy of Sciences Beijing China Abstract Based on the long-term nutrient balance experiment conducted in Yucheng Comprehensive Exp. Station of CAS the effects of nitrogen phosphorus and potassium on the photosynthetic characteristics of summer maize were investigated respectively in this study. Four treatments namely NP NK PK and NPK used as comparison are selected. In order to study the photosynthetic characteristics of summer maize in the long-term nutrient deficiency conditions the chlorophyll fluorescence parameter photosynthetic rate light response curve and CO 2 response curve of summer maize leaves are measured. The results showed that phosphorus deficiency and potassium deficiency would lead to the increase of initial fluorescence Fo. Phosphorus deficiency and nitrogen deficiency would lead to the decrease of the maximal photochemical efficiency Fv /Fm and actual photochemical efficiency Φ PSⅡ and electrons transfer efficiency ETR while the lack of potassium alone had no significant effect on the above indicators. Compared with the photosynthetic rate under the conditions of an adequate supply of NPK the deficiency of potassium has not reduce the photosynthetic rate significantly while the deficiency of nitrogen and phosphorus has lead to an significant decrease of the photosynthetic rate especially phosphorus deficiency conditions the photosynthetic rate has decreased by %. The variation of photosynthetic rate follows the same trend of Φ PSⅡ and ETR variation. The results of statistical analysis showed that there is significant linear correlation relationship between photosynthetic rate and Φ PSⅡ and ETR. The correlation coefficients are as high as and respectively. By analyzing the light response curve and CO 2 response curve it is concluded that the impact of nitrogen deficiency on the maximal photosynthetic rate saturation light intensity and saturation CO 2 concentration is more significant than the impact of phosphorus deficiency BAD89B BAD05B

1 1. Yucheng Comprehensive Experiment Station Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences

2 214 华北农学报 28 卷 Key words Summer corn Nutrition deficiency Chlorophyll fluorescence Photosynthesis rate ATP 36 57'N 'E 28 m 2 Peng 3 CO d 610 mm / mm / /12. 4% mm Fv /Fm Fv /Fo Φ PSⅡ 68. 8% NK NP PK NPK CK NPK NP NK PK 4 PK NK NP N P KNPK N kg /hm 2 P kg /hm 2 K kg /hm m ~ 20 cm 1 CO 2 Treatments / g /kg Organic matter / mg /kg Total N Tab. 1 1 Nutrient content of the soil / g /kg Total P / g /kg Total K / mg /kg / mg /kg Available P Available K / mg /kg Available N CK NP NK PK NPK μmol / m 2 s 1 ~ 1. 5 h % ~ % Li-cor CO μmol /mol

95 kg /hm 2 5 30 m 2 6 0 ~ 20 cm 1 CO 2 Treatments / g /kg Organic matter / mg /kg Total N Tab.

3 5 期李付杰等 : 长期养分亏缺对夏玉米叶片光合特性的影响 μmol / m 2 s CO μmol /mol Pn CO 2 1 ~ 1. 5 h % ~ 70% Li-cor ~ 20 cm N P K μmol / m 2 s CO 2 N P K μmol /mol P n = α 1 - βi 1 + γi I - R d 1 α β γ PSⅡ I R d PSⅡ α 8 Fo I sat P max I c 9 Fo PSⅡ R d I sat 10 β + γ /β P max I sat = 槡 - 1 P γ n max = 2 α 槡 β + γ - 槡 β ( γ ) 2 N P K N P K 3 N P K 2. 2 Fo 1-A P Fo 3 P Each value in the fig is the mean of three repeats different small letters above data are significantly different at probability levels. The same as Fig Fig. 1 Effects of different fertilization treatments on chlorophyll fluorescence

3 7 P n = α 1 - βi 1 + γi I - R d 1 α β γ PSⅡ I R d PSⅡ α 8 Fo I sat P max I c 9 Fo PSⅡ R d I sat 10 β + γ /β P max I sat = 槡 - 1 P γ n max = 2 α 槡 β + γ - 槡 β ( γ ) 2 N P K N P K 3

4 216 华北农学报 28 卷 PSⅡ NPK ETR P N K NPK Fo N P N P NPK Fo N K P NPK 2. 3 PSⅡ Fv /Fm PSⅡ NPK N P PSⅡ P 11 Fv /Fm PSⅡ % Φ PSⅡ 2 ETR Φ PSⅡ ETR 1-B K NPK N P K N P NPK Fv /Fm 2 K PSⅡ Φ PSⅡ PSⅡ 12 PSⅡ Φ PSⅡ PSⅡ 13 1-C N P 2 NPK Φ PSⅡ Fig. 2 Photo rate of different fertilization treatments N P ETR 2 PSⅡ K NPK K Φ PSⅡ ETR 1-D Fv /Fm Φ PSⅡ K Tab. 2 2 ETR Φ PSⅡ Fv /Fm Correlation analysis of photo rate and ETR Φ PSⅡ Fv /Fm ERT Φ PSⅡ Fv /Fm Correlation coefficients Significance Fig. 3 3 ETR Regression equation of photo rate and ETR Fig. 4 4 Φ PSⅡ Regression equation of photo rate and Φ PSⅡ

2 Photo rate of different fertilization treatments N P ETR 2 PSⅡ K NPK 0. 988 0 0. 001 8 K Φ PSⅡ 0. 986 0. 002 ETR 1-D Fv /Fm Φ PSⅡ K 0. 821 Tab.

5 5 期李付杰等 : 长期养分亏缺对夏玉米叶片光合特性的影响 y = x R 2 = y = x R 2 = μmol / m 2 s P NPK N 2. 4 P μmol / m 2 s NPK NPK μmol / m 2 s N P N P μmol / m 2 s N Fig. 5 Light response curve Treatments Tab. 3 3 Fo Saturation intensity Parameters of light response curve Max photo rate Light compensation point Correlation coefficient NPK NK PK CO 2 CO P NPK CO 2 N CO 2 N NPK CO 2 N CO 2 NPK P P NPK CO N P K Fo P Tab. 4 Treatments 6 CO 2 Fig. 6 CO 2 response curve 4 CO 2 Parameters of CO 2 response curve CO 2 / μmol /mol Saturation CO 2 concentration Max photo rate NPK NK PK Correlation coefficient 3 N Fv /Fm Φ PSⅡ ETR F O PSⅡ 14 F O D1 N P PS Ⅱ ETR 12 PSⅡ Fv /Fm PSⅡ Φ PSⅡ 15 PSⅡ Fv /Fm

3 3 Fo Saturation intensity Parameters of light response curve Max photo rate Light compensation point Correlation coefficient NPK 0. 032 996 1 753. 43 28. 65 24. 32 0. 999 8 NK 0. 067 884 1 614.

6 218 华北农学报 28 卷 Φ PSⅡ na ATP D Calvin 5 Krause G H Weis E. Chlorophyll fluorescence and photo NADP + synthesis J. Ann Rev Plant Physiol Mol Biol 1991 NADPH NADP + PSⅡ 6 Van Kooten O Snel J F. The use of chlorophyll nomencla2ture in plant stress physiology J. Photosynth Res PS Ⅱ PSⅡ J Ⅱ K NPK J RUBP J RUBP 10. N P J P % 11. J J Genty B Briantais J Baker N. The relationship betweenthe quantum yield of photosynthetic electron transport 24 CO 2 and quench2ing of chlorophyll fluorescence J. Biochim Biophys Acta CO J Φ PSⅡ ETR J Φ PSⅡ ETR Jacob J Lawlor D W. In vivo photosynthetic electron transport does not limit photosynthetic capacity in phosphate_deficient sunflower and maize leaves J. Plant- Cell and Environment Fredeen A Raab T K Rao I. Effects of phosphorus nutrition on photosynthesis in Glycine max L. Merr J. Planta Li X Ashihara H. Effects of inorganic phosphate on sugar catabolism by suspension-cultured Catharanthus ro- seus J. Phytochem Jacob J Lawlor D W. Stomatal and mesophyll limitations of photosynthesis in phosphate deficient sunflower maize and wheat plants J. Journal of Experimental Botany _ _ J J Rubisco RCA J J Qi H Yu G R Cheng Y S. Effect of potassium fertilization J. on the photosynthetic characteristics of the leaf of winterwheat population during its grain filling stage J. Appl Ecol J Peng C L Lin Z F Lin G Z. The antiphotoxidation of anthocyanins-rich leaves of purple rice cultivar J. Sci Chi-

Photosynth Res PS Ⅱ 1990 25 147-150. PSⅡ 7. 3. 2 J. 2007 38 6 771-775. 8. Ⅱ K NPK J. 1994 36 2 93-98. 20-21 RUBP 9. 22 J. 1999 25 1 29-37. RUBP 10. N P J. 2000 34 3 248-251. P 32. 86% 11. J. 2004 24 5 812-816.

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214 华 北 农 学 报 28 卷 Key words Summer corn Nutrition deficiency Chlorophyll fluorescence Photosynthesis rate ATP 36 57'N 'E 28 m 2 Peng

214 华北农学报 28 卷 Key words Summer corn Nutrition deficiency Chlorophyll fluorescence Photosynthesis rate ATP 36 57'N 'E 28 m 2 Peng