1 2 3 4 5 6 7 8 9 DNA DNA DNA DNA
8 A B C D E
8 A
8 B
5- -1- PRPP AP AP UMP GMP HMP HPRT HR AMP TMP TK TR
TK tk - HAT tk HPRT hprt - HAT hprt
APH - G418 APH G418 DHFR DHFR HPH - B B HPH B TK - TK TMP XGPRT - XGPRT GMP ADA - ADA Xyl-A
CHO FDA GRAS
8 C DNA DNA SV40 DNA BKV DNA
DNA DNA 93 6 C 2 Ad2 5 Ad5
DNA DNA IVa2 VA L1 L2 L3 L4 L5 ITR ITR E1 E2 E3 E4 DNA 36 kb 105% DNA ITR E1-E4 E3 L1-L5 IVa2 VA RNA E3 2.2 kb 4 kb
DNA DNA DNA DNA
SV40 DNA SV40 SV40 VP1 VP2 VP3 5224bp DNA SV40 H2 H3 H4 DNA SV40
SV40 DNA DNA SV40 t / T tt ori VP2 VP3 SV40 T SV40 DNA 105 DNA VP1
DNA SV40 SV40 DNA- DNA SV40 DNA DNA SV40 SV40 pbr322 ori Ap r pbr322 pv2-gpt SV40 SV40 ori viral gene pv2-gpt SV40 DNA DNA gpt - β-. gpt
DNA SV40 SV40 DNA SV40 ori DNA SV40
SV40 DNA DNA SV40
DNA BKV BKV DNA 500
DNA BKV P450 dioxin BKV gene DRE MMTP BKV ori BKV - E.coli Ap r Neo r SV40 P E.coli ori DRE MMTP SV40 Neo r G418 β1- B
DNA DNA 185 kb DNA
DNA DNA T7RNA T7RNA T7 DNA T7RNA
DNA T7 T7 RNA
8 D
DNA
DNA CaCl 2 DNA 37 4-16 DNA DNA 7 DNA DNA DNA
DNA DNA
DNA DNA DNA HeLa
DNA
DNA DNA DNA
DNA
8 E VIII
MTX DHFR dhfr dhfr dhfr DNA
DHFR-MTX 0.05 µm MTX dhfr - dhfr 0.25 µm MTX 5 µm MTX 100
GS-MSX GS MSX GS GS MSX GS MSX GS-MSX DHFR- MTX
CMV SV40 polya mrna mrna ColE1 ori CMVP Polylinker Gene Ap r Intron AAAAAAAAA M13 ori SV40 T PolyA signal mrna mrna
300 β VIII EPO 2 IL-2 tpa γ IX hgh CD4
SV40 dhfr pl β campath campath β cdna Neo r ph β DHFR-M TX β cdna
tpa DHFR-MTX CHO tpa tpa cdna dhfr tpa
VIII VIII VIII 10% VIII cdna tpa VIII VIII