2007.3 1
2...1 VB2...4...7...9...12...14...17...19...19...20...26...31...33...37 B1...44
1. 2 3. ph= 3-9( ph=5-6) lgk =21. 3. 510nm, 1.1X10 4 Fe 2+ - 1. 1 cm l ml2m15ml 25ml 2 1. 000? 10-3 mol/l 0.4822g 80ml 1+1 1000m1 1.5g/L 0.15g l00ml. 100g/L 1mol/L 0.1mol/L 1
1. 2 25ml 0. 001. 00ml 1. 000? 10-3 mol/l, 0.5ml 100g/L lml 1.5g/L 2.5ml 1mol/L 1cm 44Onm 560nm 5nm 2nm 2 6 2 5 ml 1. 00ml 1. 000? 10-3 mol/l, 0.5ml 100g/L 0.000.10 0.250.501.002. OOml 1.5g/L 2.5ml 1mol/L 3. 7 25m1 1. 00ml 1. 000? 10-3 mol/l, 0.5ml 100g/L 1.00ml 1.5g/L 0.002.505.007.5010.0015.0020.00ml 0.1mol/L ph 7 25 m1 ph ph 4. 2min,5min, 10min,15min,30min,60min120min 5 6 25m1 0.00 0.20 0.40 0.60 0.801. 00ml 1. 000? 10-3 mol/l, 0.5ml 100g/L 1.00ml 1.5g/L 2. 5ml lmol/l 2
1. 2 Fe 2+ 1. 2 3
VB2 1. 2. 3. V B2 F C F= KC 430-44Onm 535nm ph6-7,ph=11 V B2,V B2 V B2 0.8-1. 930 1. Ocm, 8cm) 50ml1000m1 10ml, 50m1 l ml, 2m1 2. V B2 25mg/L 25.0mg/L 400m1 1-2m1 1000m1 V B2 (lug/ml) 25mg I 2. OOml 50m1 0. lmol/l HAc ( 5 0 mg/l 50. Omg, 1000m1, 0.05mg/L =529 0.1mol/L HAc 4
60-100 1. V B25 mg 2. (1) 1.5g 1/2-2/3, 60-80 / 2) 6 1µg/ml V B2 0000. 501.00 1. 50 2. 002. 50ml 15-20ml 60-70 (3) l0ml 5m1 5ml l0ml. (4 100nm 510nm 0 0 20 V B2 100 100( 90 0100 F (5) F=-F S -F 0 V B2 3. 0. 50m1. OOml 4. F X =-F X -F 0 V B2 V B2 V B2 (mg/l= V B2 / V B2 V B2 1. 25mg 350µg 5
1. 2. 3. V B2 6
1. 2 A C Lamhert-Beer A=KLC L K A=KC 1. 5m1 25ml 2. 12.5mmol/L 0. 8173g (1 : 1) 1000m1 1 V /V) 50µmol/L 12. 5mmol/L 1. 50µmol/L 0. 00 0. 50 1. 00 1. 50 2. 002. 503. 00m1 25ml 0. 001. 002. 003. 004. 005. 006. OOµmol/L 2. 2-5 7
8 3. Zn 5m A 213. 9nm 15 Zn 4 A µmol/l 1. 2. (11 1. 2.
1. 2. (SCE) E E=K-0.059 1ga F (25 (TISAB),. E C E=K-0.059lgC F C F TISAB NaClHAc-NaAc NaCl HAc-NaAc ph 5.0-5.5 Al 3+ Fe 3+ Th 4+ 1. p H S-2 50m1 1000m150m1 lml2ml 5ml10ml 20ml 50ml 2 (1 0mgm1) NaF( 105110 2h)0.2210g 100ml (100µgm1) TISAB 58gNaCl57ml 10g 500ml 5molL NaOH 120ml 5molL NaOH ph=52 1000ml 5molL NaOH 9
1 10Oµgm1 O51O2050100200ml 50ml TISAB 25ml ( ) 2 ( ) 3 (my)( ) ElgC F EC F 4 (1)20ml 50ml TISAB 25ml (2) (3) E 1 (mv) (4) 1000µgm1 O20ml(Vs) E 2 (mv) E(=E 1 E 2 )10 40mV 5 (1) E 1 C F F C F (2) C(mgL)=C 1 V 1 /V 2 (10 E/S - 1) -1 C C 1 V 1 V 2 s (mv) 1 10
2 3 1 TISAB? 2? 11
1 2 ( ) 3 ( )G G=/ 25"C 548 10-6 sm 1 10-4 Sm (DDS ) 1 ( ) 20 0100molL KCl 120 4 KCl(GR) O7456g ( <1 10-5 Sm) 1000ml O0100molL KCl 1 220V 30 2 ( ) 100 ( 12
13 S) 3 O0100molL KCl (G) =K KCl /G KCl (K KCl KCI ) 4 (K Sm) 1 (<5µS) ( 5µS150mS) U 2 3 1 2 25 4 5 KCl 1? 2? 3? 4??
1 2 ( ) ( ) ( 1 ( ) 34mm 2m 3 25 101 (102 )6080 10ul 10ml 2 ( ) CS 2 100ml lml 40250ml CS 2 11ml 5 10ml 5ml CS2 4647 1 CS 2 (Vml)100ml 20ul CS2 100ml 2 14
(1) 78 180 140 N225mlmin H260mlmin Air550mlmin (2) 3 100ml 115ml ( 08777)115ml ( 08650)250ml ( 1107) CS2 1009mgml 995mgml 2768mgml 5 100ml 10 20304050µl 20µl CS 2 (3) 1 5 1µl[12] ( ) ( ) ( ) 1µl 3 ( ) (mgm1)=w X lov w (mgm1)v (m1) 4 15
1CS2 2 1 2 16
1 HPLC 2 179 18 371 C 18 254nm C 1 2 ( ) (GR) K 2 HP0 4 3H 2 0 (AR) 1 100ml HCl 004ml O3g 400ml 30s 5min 020ml 60 N 2 O20ml 2 A K 2 HP0 4 3H 2 0 45g 2L 10ml B AB O5um 3 C 18 A B=8020 lmlmin 254nm 4 17
01500g 01molL NaOH 200ml 50ml 500ml 150mgml 5 O 00O 20O 40 0 60 080100ml 100ml 5ul 6 5ul 7 (1) (2) n=554(t R W 1/2 ) 2 1? 2 A B?? 18
19
20 (g) (m1) (cm 2 ) (m 3) 35~37 24h (35~37) (44~45) 9 () () 510min
13min 80 () 500ml 1.5 2ml lo13kpa 20min () 1015cm 5min () 2h 4h lml 3724h ( 1 1) lml 100 () 1 2 lml 1 3 3 (1) 2025 (2) lml 15ml 21
20ml 45 2 (3) 37 24h lml 1 2 2 (1) 30300 ( 1-2 1) (2) 30300 ( ) 2 ( 1-2 2 2 ( 1-2 3 4) (3) 30300 ( 1-2 5) (4) 30 ( 1-2 6) (5) 30300 300 30 ( l-2 6) (6) 1 ( 22
23 l-2 7) (7) 100 100 10 ( 1-2 ) 1 2 lml ( ) 3 45 4 30min 1? 2? 3? ()
1 2 lml 3 loml 2 looml 1 9ml 1 90ml 1 3 3 1 (1) 3 1) 2 50ml 3 ( ) looml lo 5ml 3 ( ) loml 37 24h 2) 24h 37 18 24h 3) ( ) 13 37 24h 24
( ) () 1-4 25
35um lum 8NaCl ph l2 4 99 3 15 3716h 24 10 7 ml 1002025min 100 30min 120 60min 75 5 48h 1 2 4 () (1.0ml 10.0m1) (30 36±1 ) (MYP) L- (TSSB)Butterfield () ( 4 3) 26
1 6 4 4d -20 2 (1) 50g (2)450ml 18 00020 000rmin 2min 110 (3)1 10 10.Oml 90.Oml l100 (4) 1 10.Oml 10 10 6 3 (1) 0.1ml MYP L MYP (2) 30 24h. (3) 15150 MYP 24h 27
(4) 5 30 24h ( ) (5) 10 10-4 0.1ml MYP 25 5 4 1g (25*45) 10 4 10=2 10 6. 4 5 (1) 35 24h ( ) (2)L- L- 35 48h ( ) 72h (3) 2mm 35 24h (0001 ) 24h (4) MYP 35 24h ( ) 6 (1) 30 24h (2) 30 18 24h 28
(3) 3032 24h B (4) 30 24h 23d O.5 ( ) 1.5min 0.5min 23d 7 (1) O.95115 15min 30 1820h (2) 1) 1015cm 6 2cm 2ml68h (VL)O.5 2) (2025g) 0.10.4ml 12h 1min 29
30
JWL I JWL I Anderson 9cm 1 1.21.5m 5 20min 2 37 24h 3 5 cfu 5 100mm 2 5min 10L ( ) A (cm 2 ) t (min) N 31
1 9cm 2 3 32
33 42 1 2 3 3 1 SCDLP 2 ()
() 1 (1) ( ) 10g 90ml 10ml 5ml 45ml 110 (2) ( ) 10g 10ml 10ml 80 4044 10min 75ml 4044 110 35min ( 1 10) (3) 1) 10g 90ml ( ) 3032 15min 1lO 2) 10g 10ml 10ml 80 70ml 4044 110 (4) 10g 90ml 34
3032 15min 3032 15min 110 10g 90ml 12min; 10g 90ml SCDLP 1g lml lml 807ml 35min 2 110 10ml 90ml SCDLP 37 1824h 3 37 1824h 37 24h 4 5 1 1 1530s 6 23 37 24h 35ml lmoll lml 7. 37 24h 35
36 8. 37 24h 1030min 9.42 4142 2448h 42 1? 2? 3? 4
l. 2 3 1. (2528) (300m1) (15mm*150mm)( 9cm) (1ml 10m1) (121 20min) 2 (1) ( GB 4789.28 4.79 ) _ ( ) 45 (2) (GB 4789.28 4.81 ( ) (3) (GB 4789.28 4.78 ) 1 ( 3 1) 37
2 (1) 250500g ( ) 250g ( ) 500g 10 000 (2) ( ) 25g(25m1) 225ml 30min 110 1 10 loml lml 50 lml 110 9ml lml 5 1100 10 lml (3) 3 10 lml 2 38
45 2528 ( 2528 37)3 5 l. 10150 2 ( ) 2 g(m1) (cfu) 1. 10150 30300 5 3 2 1. (2528) (150m1) (15mm*150mm) ( 9cm) (18mm*18mm) (121 20min ) 2. (GB 4789.28 4.77 ) -- ( GB 4789.28 4.79 ) ( GB4789.28 4.80 ) (GB 4789.28 4.82 ) (GB 4789.28 3.24 ) 1 ( 3 2) 39
2 (1) 250500g (2) 1020g 150ml 12cm 12min 10 75 l2min (3) ( ) 10 ( ) 5 100 2528 57 1 100 2 12 40
41 25 28 514 3 (1) (2) ( ) ( ) (3) (4) (5) (6) ( )
42 (Aspergillus) (Penicillium) ( ) (Fusarium)
43 O5 1Ocm O305cm O1O2cm 12(3) 4 1 2 3 9cm 2528
44 4 5 95 47 B1