1. 530011 2. 410007 KEGG HMDB metpa 9 15 CoA P 约 0.05 CoA CoA doi: 10.11842/wst.2014.07.029 R256.2 A [1~4] GC-MS metpa KEGG Kyoto Encyclopedia of Genes and Genomes HMDB The Human Metabolome Database [5] 1 1.1 L-Lactic acid Beta-Alanine L-Valine Succinic acid 2-Propylmalate Stearic acid Arachidonic acid D-Fructose 1.2 吟 KEGG 吟吟 HMDB 吟吟吟 metpa 2013-10-27 2013-11-14 元 81102595 BMSCs 1347004-26 BMSCs 201203YB113 2012 GZPT1219 Z2012181 元元 吟 http://www.genome.jp/kegg/ 吟吟 http://www.hmdb.ca/ 吟吟吟 metpa.metabolomics.ca/ 1614
2014 绎 Vol.16 No.7 1 HMDB ID KEGG ID L-Lactic acid HMDB00190 C00186 5-30 Beta-Alanine HMDB00056 C00099 16 1 28 L-Valine HMDB00883 C00183 6 1 54 Succinic acid HMDB00254 C00042 44-77 2-Propylmalate - C05994 - - 4 Stearic acid HMDB00827 C01530 71 1 11 Arachidonic acid HMDB01043 C00219 113 2 39 D-Fructose HMDB00660 C00095 15-22 2 MetPA Total Cmpd Hits Raw p Impact Details Pantothenate and CoA biosynthesis 15 2 0.002 884 0.0 KEGG Propanoate metabolism 20 2 0.005 145 0.0 KEGG Biosynthesis of unsaturated fatty acids 42 2 0.021 884 0.0 KEGG Valine leucine and isoleucine biosynthesis 11 1 0.061 219 0.333 33 KEGG Fructose and mannose metabolism 19 1 0.103 66 0.132 47 KEGG beta-alanine metabolism 19 1 0.103 66 0.444 44 KEGG Butanoate metabolism 20 1 0.108 84 0.0 KEGG Citrate cycle TCA cycle 20 1 0.108 84 0.025 66 KEGG Pyruvate metabolism 22 1 0.119 13 0.0 KEGG Alanine aspartate and glutamate metabolism 24 1 0.129 32 0.0 KEGG Glycolysis or Gluconeogenesis 26 1 0.139 4 0.0 KEGG Galactose metabolism 26 1 0.139 4 0.0 KEGG Arachidonic acid metabolism 36 1 0.188 31 0.326 01 KEGG Valine leucine and isoleucine degradation 38 1 0.197 79 0.0 KEGG Pyrimidine metabolism 41 1 0.211 83 0.0 KEGG Fatty acid biosynthesis 43 1 0.221 07 0.0 KEGG Aminoacyl-tRNA biosynthesis 67 1 0.324 81 0.0 KEGG Total Cmpd Hits Raw P P Impact 2 2.1 HMDB KEGG 8 ID 1 2.2 MetPA 8 17 2 1 CoA 1615
茁 - TCA Overview of Pathway Analysis 6 5 4 -trna 3 P 约 0.05 3 CoA Pantothenate and CoA biosynthesis Propanoate metabolism Biosynthesis of unsaturated fatty acids 3 2~4 3 3 2 1 0.0 0.1 0.2 0.3 0.4 Pathway Impact 1 MetPA -Log P Beta-Alanine C00099 L-Valine C00183 CoA CoA Acyl Carrier C06002 C00099 C05985 C00024 C05984 C00831 C00429 C00183 C00864 C02642 C00106 C00141 C00100 C00222 C00804 C00083 C00332 C00109 C03492 C04079 C00097 C00099 C04352 C00683 C05983 C00894 C01013 C01134 C01213 C00163 C05668 C00042 C00882 C00010 C00091 2 CoA KEGG ID 3 KEGG ID 1616
2014 绎 Vol.16 No.7 C16645 C16532 C16531 C16530 C16529 C16528 C16170 C02050 C02249 C03595 C03035 C16533 C08323 C08316 C16526 C08320 C08281 C16527 C16525 C16513 C16522 C00249 C16169 C16165 C16162 C02050 C02249 C03595 C03035 C16169 C16165 C16162 C01530 C06425 C00712 C01595 C00219 C03242 C06426 C06429 C06428 C06427 4 KEGG ID Protein ACP PanB PanE L- - 琢 - PanD PanC 4 CoA CoA 90% [6] [7] CoA Beta -Alanine C00099 Stearic acid C00042 CoA A 3 A 茁 - A D- A L- A B 12 A L- A A A 琢 - A A A A ATP [8] Arachidonic acid C00219 Stearic acid C01530 A A CO 2 H 2 O 玉 域 1617
1,,,. -.,2009,27(4) 颐 796~798. 2,,,..,2012,32(4) 颐 515~520. 3,,,..,2010,30(6) 颐 579~584. 4,,,..,2010,51(8) 颐 729~732. 5 Xia J, Wishart D S. MetPA: a web-based metabolomics tool for pathway analysis and visualization. Bioinformatics, 2010, 26(18) 颐 2342~2344. 6 Genschel U. Coenzyme A biosynthesis: reconstruction of the pathway in archaea and an evolutionary scenario based on comparative ge 原 nomics. Mol Biol Evol, 2004, 21(7) 颐 1242~1251. 7,..,2008,28(4) 颐 448~452. 8,,,..,2012,34(7) 颐 81~87. Analysis on Plasma Metabolicomics Pathway inratswithheartbloodstasissyndrome Zheng Jinghui 1,MoYunqiu 1, Jian Weixiong 2,NingGuilan 1, Zhang Yaping 1, Zhu Zhihua 1,WeiZhuo 1, He Jing 1, Wang Tiehua 1 (1. Ruikang Hospital A ffiliated to Guangxi University of Chinese Medicine, Nanning 530011, China; 2. Institute of Chinese Medicine Diagnosis, Hunan University of Chinese Medicine, Changsha 410007, China) Abstract: This study was aimed to analyze the plasma metabolicomics pathway in rats with heart blood stasis syn 鄄 drome. KEGG database was used in the signal pathway analysis. HMDB was used in the analysis of molecular metabolite annotation, enzyme or transporter associated and its related properties. The metpa network software was used in the visualization of metabolite path. The results showed that 9 metabolites involved in 15 metabolic pathways. Among them, the P -value of metabolic pathway of pantothenate and CoA biosynthesis, propanoate metabolism, biosynthesis of unsaturated fatty acids was less than 0.05. It was concluded that the metabolic pathways of pan 鄄 tothenate and CoA biosynthesis, propanoate metabolism, biosynthesis of unsaturated fatty acids were involved with the pathological process of rats with heart blood stasis syndrome. Keywords: Metabolicomics, heart blood stasis syndrome, metabolic pathway, pantothenate and CoA biosynthesis, propanoate metabolism, biosynthesis of unsaturated fatty acids 1618