5 8 0 8 9 Hubei Agricultural Sciences Vol. 5 No.8 Sep.0 ( / 4000) : 目前市场上生产的蛹虫草产品没有统一的质量标准 影响市场对蛹虫草产品的消费 研究结果表 明 蛹虫草菌种 H z 孢子复壮的菌株培养的子实体干重高于菌种 B B z H 孢子复壮的菌株培养的子实 体干重 其液体培养基最优组合为葡萄糖 0 g / L 蛋白胨 8 g / L KH PO 4.0 g / L MgSO 4 500 mg / L 生产富 硒蛹虫草培养基的最优主料为富硒大米 g 培养液 55 ml 在培养温度为 空气相对湿度 70% 时 蛹虫草子实体生长最好综合而言 子实体培养最优条件为日间温度 夜间温度 5 空气相对湿 度 70% 光照度 00 lx 并结合先用日光灯照射 待子实体长至 ~ cm 时使用蓝紫灯照射处理的产量最 高检测结果显示 蛹虫草子实体的有效成分含量分别为硒 5.0 mg / kg 蛋白质 7.74% 腺苷 0.05% 多 糖.50% 虫草素.6% 氨基酸 6.9% 尤其是所含的虫草素具有工业提取价值 : 蛹虫草 ; 质量 ; 产量 ; 技术参数 ; 优化 ; 生产技术 :S567. + 5;Q9-;Q99.9 :A :049-84(0)8-4069-07 Integrated Production Technology for Improving the Quality and Yield of Cordyceps militaris YANG QangLIU Jin-longZHENG Xiao-jiangCHEN Yao (School of Biological Science and Technology/Hubei Key Laboratory of Biological Resource Conservation and Utilization Hubei Institute for Nationalities Enshi 4000 Hubei China) Abstract: There has not been any uniform quality standards for production of Cordyceps militaris (L.Fr) Link in market which affect the consummation of goods. The study results showed that fruit body dry weight of H z spore rejuvenation strain was higher than that of B B z H spore rejuvenation strains. The optimal liquid medium contained glucose 0 g / L peptone 8 g / L KH PO 4.0 g / L MgSO 4 500 mg / L. The best main substrate in medium for production of selenium enriched C. militaris was selenium enriched corn g + medium 55 ml. The fastest mycelium growth rate could be obtained under and 70% humidity. Optimal conditions for fruiting body cultivation to get the highest yield was at daytime and 5 at night humidity 70% illuminance 00 lx combined with fluorescent light firstly and then blue -violet rays when length of fruiting body was ~ cm. The content of selenium protein adenosine polysaccharide cordycepin and amino acids in fruiting body was 5.0 mg / kg 7.74% 0.05%.50%.6% and 6.9% respectively among which extraction of cordycepin was with industrial value. Key words: Cordyceps militaris (L.Fr) Link; quality; yield; technical parameters; optimization; production technology [Cordyceps militaris (L.Fr) Link] (Ascomycota) (Hypocreales) (Clavicipitaceae) [Cordyceps (Fr.)Link] [C. [] sinensis (Berk.) Sacc.] ; [] :0-08-8 : (cxy009b07); 0 (SKY049) : (989-) 007 ( )50784664( )4090@qq.com; ( )40788( )liujl68@6.com
4070 0 PDA 0 g 0 g 5 g 0 g [-6] ( )PDA 4 500 ml 50 ml 4 B B z H H z 4 5 ml [7-0] 5 0~80 r / min 7 h 7 h... 菌种 4 B ( )H 4 ( ) )B z ( PDA 5 500 ml )H z ( 50 ml 5 [] ) B B z H H z 4 5 ml.. 培养基及培养主料原料 80 r / min 7 h PDA ( ) KH PO 4 MgSO 4 Na SeO ( ) 5 60 [] B (VB ) ( );.. 液体培养基优化 L 9 ( 4 ) 正交试验设计.7 mg / kg KH PO 4 MgSO 4 ( ) 4.. 仪器 5 ml LRH-ZSO 0~80 r / min 7 h H z DX- BT5S 80- PHS-5 ph LC-0 ATupplus YXQSG4680 IOP- KFR-SN5 //g/l) WS00A GXMQ 0 CO HP00 0 0..4 最优培养主料比较试验... 碳源对培养基优化的试验设计 + ( )PDA :00 g g 000 ml 0 min + 0 g 0 g KH PO 4 g MgSO 4 5 60 [].. 氮源对培养基优化的试验设计 ( )PDA 0 g 5 g 50 g 0 g 0 g 5 0~ L 9 ( 4 [] ) H z.. 500 mg B [] ( ) 55 65 ml; L 9 ( 4 ) A B C D ( ( //g/l) (KH PO 4//g/L) (MgSO 4//mg/L) 8 0 0.5.0.5 00 500 800
8 : 407 //ml //g //g //g //g //g //g 55 65 + + + H z 5 ml [4] 50 d A..5 蛹虫草孢子复壮培养 ( // ) B B z H H z 4 8 ( ) 4 [5] 4 h // ) 7 h 8 0~80 r / min 0 50..6 培养基灌装程序中灭菌控制设计及接种器 具比较 d..9 不同光源对蛹虫草 H z 菌种子实体生长及产 (800 ml) 量的影响 50 H z : 04 ; ; ;4 00 (0 min) ~ cm ; (0 min+0 min); 5 ~ cm h 5 min ;6 ~ cm ; 75% ;7 75% ~ cm 50 [67] [0]..0 0% 分检测 蛹虫草 H z 菌种和天然冬虫夏草子实体成 H z 0 / min 0 / min; H z H z ;HP00 [] [5] ; ATP ;..7 温度 空气相对湿度对蛹虫草 4 个菌种子实 [] 体培养管理参数研究设计 [8] [5] 5 8 0 4 5 ; 60% L 4 ( ) A L 9 ( 4 ) B ( ( // )( //%)( //lx) 65% 70% 75% 80% 5 ;4 B B z H H z. L 4 ( ) () [9]..8 温度 空气相对湿度 光照度影响蛹虫草 H z 5 5 B 菌种的子实体产量正交设计 0 g / L B z 4 5 g / L H 0 g / L L 9 ( 4 ) 4 H z 0 g / L 5 8 0 B ( //%) ( ) 65 70 C 60 65 70 C B z H z D 00 00 00
407 0 5 4 H z 5. g / B z B H 4.5% 0.9%.0% :A>B>D>C; (g/) A B C D 0 g / L 8 g / LKH PO 4 0 g/l 0 g/l 5 g/l 0 g/l B B z H H z. 5..0 6.0. 5.0.6 5.5.8 5.40.0 5.80.4.68.00.0. 55 ml 5.6 g / ; g 65 ml 6 6 B B z H H z 5. g / 0 g / L 4 ; H z 6.%.5% 0.8% 6 B z B H. H z L 9 ( 4 ) H z L 9 ( 4 ) 7 7 4.0 g / LMgSO 4 500 mg / L (g/) 0 g/l 5 g/l 50 g/l 0 g/l 0 g/l B B z H H z 4.0 6.40.0 7.40 7 4 5 6 7 8 9 x x x R. 5.80.80 6.5.4.40.40 5.8.0.00.06.0.4 5.0.90 6.80 L 9 ( 4 ) A 6.0 6.04 0.60 5.4 B 5.40 4.607.860.80 C.887 4.60 4.0 0.7 D 4.57 4.947.60.44 //g/ 6.6 7.50 4.0 6.78 6.0 5.0. 0.0 9.55.4 H z 6 H z g 55 ml ; g g ml.50 g / ; g 55 ml 4.00 g / ; + g ml.0 g / 6 //g/ 6 5 4 0 g+ ml g+55 ml g+65 ml + +.5 4 4 B B z H H z 8 8 H z B z B H.%.8% 94.0% H z 4.6 H z H z
8 : 407 8 B B z H H z //g/ 4.0 5.04.68 5.0 9 9 00 00 0.08%; 0 min 0 min.7 H z 00 min 0.0%; 0.0% H z 00 0 min 0 0 9 H z // //min //% ; H z H z 00 0 0.0 H z 00 0.0 0 / min H z 00 0+0 0.08 ///min 0 0 0 //% 0.0 0.0.8 4 5.0 4.5 4.0.5 4.0.5 4.0.5 4.0 0.5 0 60 65 70 75 80 70%4 //% 4 H z 4.88 g / ;B z 4.50 g / ;B.4 g / ;H. g /.9 H z L 4 ( ) L 9 ( 4 ) H z L 9 ( 4 ) C>A>B A B C 70% H z A>D>C>B //g/ 5.0 4.5 4.0.5.0.5.0.5.0 0.5 0 5 8 0 4 // A B C D H z 5 70% 00 lx //g/.0 H z H z 6( ~ cm ) 5.0 g / ; 7( ~
4074 0 L 4 ( ) A( // ) B( //%) C( ) //g/ 4.04 5.0 5.6 4 5.00 x 4.50 4.700 4.50 x 5.80 5.00 5.90 R 0.650 0.0 0.670 H z L 9 ( 4 ) 4 5 6 7 8 9 x x x R A( // )..940 4.607.94 B( // ) C( //%) D( //lx) 4.60.77.97 0.4.647.967 4.47 0.600.947 4.5.560 0.79 //g/.0.50.4.58 4.00 4.4 5..68 4.8 cm ) 5.8 H z g / ; 5( ~ cm ) 5. //% //% //% g / ; 4( ~ cm..7 7. ) 5.04.5.05 4.8 0.9 0.0 96.7 g / ; ( ) 4.0.79 05.8 4.76 g / ; ( ) 0.95.5 76.0 4.48 g / ; ( ).6 g /.60.4..48 44. 6.5 ~ cm 0.47 0.5 88.0.55. 8.4 0.6 0.5 7.. H z 0.48 0.60 80.0.0.07. H z 0.89 0.65 6.9.60. 4.6 0.99. 8.8 (.79 0.78 9.5 ).0 0.9 0.9 H z.58 8.%.% 6.9.0.57 8.4 4.8 9.5% 0.9% 7.% 8.7% [] 4.8% 8.4%.% ( 0.0% ) H z
8 : 4075 4.8% 70% H z. H z 5 70% 00 lx ~ cm H z 4 4 Se 5.0 mg / kg 7.74% 868.9%Mn 5.%; Ni 70.4%Cu 59.%Zn 65.8%Fe 6.% K [8] 44.7%Ca 60.% [45] ; Cu [6] Cu ;Ni Ni [7] Ni : []. [J]. 0047 K Ca Se Fe Zn Cu Mn Ni. H z 0098():6-64. H z [9] 7.74%.50% 0.05% 0():9-95..6% 0.0% H z [J]. 004():6. [4] H z [5] B B z H 9976():. 0 g / L 8 g / L KH PO 4.0 g / L MgSO 4 500 mg / L g 55 ml; 6.9% 0.05%.50%.6% ():5-7. 4 H []. z [J]. 00(4):-. []. [D]. : 007. //mg/kg //mg/kg //% 7 500 50 5.0 09.00 88.00 8.0 46.90. 400 69 5.7 58.00 57.00 9.80 0.60 7. 54.7 9.7 968.9 8.8 4. 40.9 5. 9.6 [4]. [J]. 0074():5-57. [5].HPLC-ESI-MS [J]. 0049(8): 76-764. [6]. [J]. 00():6-6. [7]. [J]. ( )996(4):79-8. [8]. [J].. [J]. 009. [0] [J]. 0090(7):66-69. []. [J]. 004(4):. []. [J]. 987():. [].. [J]. 0098(5):699-704. [6]. [J].. [J]. 000(4):9-0. [7]. [J]. 00(4):56. ( 下转第 408 页 )
8 : 408 [9] PRL 0.04 μg / L; PRL ) ( ) ( ) : []. [M]. : 00. []. [J]. 005(6): 0 d 5-6. [6] 5- []. [J]. (4.0± 009(5):56-58..58) d [4] [M]. (7.00±.6) d 9.0 d : 006. [7] ( ) ( ) ( ) 9947():75-78. [8] [7]. (5- ) [J]. 005(7):5-7. [8] [J]. 994():. ) [9] 997(4):8-9. ( 责任编辑程碧军 )!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! ( 上接第 4064 页 ) []. [J]. ( [J]. 008 (): 8996-9000. []. [J]. [J]. ( )0076 ():07-00():5-7.. [] 0(5):-5.. [J].[6]. [4]. ( 上接第 4075 页 ) [8] )0070():5-8.. 000():4-44. [J]. 0075(9):99-994. [9]. (Cordyceps militaris) [J]. 998(): [5] 5-40. [5]. [J]. 008(0):-6. ( 责任编辑王珞 ) [0]. [6]. [J]. 995():5-8. []. 7. [J]. 0045(5):78-79. []. 997(6):64-65. [J]. 9998():8-9 []. [J]. [4]. [J]. 006():-5.. [5]. PRL P 7β E [J]. 996():-6. [6]. 5- [J].. [J]. ( )005():5-7. [J]. 004():5- [7]. [J]. [8]. [J]. 0055(9):86-867. 5-.. [J].!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! ( 责任编辑王珞 )