Hic-5 vinculin structural protein 60 PY LD 1 LD 2 LD 3 LD 4 LIM1 LIM2 LIM3 LIM4 Pyk2 FAK Csk GIT PKL Nuclear localization Focal adhesion localization PTP-PEST HSP27 tyrosine kinases Arf-GAP tyrosine phosphatase Paxillin LD 1 LD 2 LD 3 LD 4 LD 5 LIM1 LIM2 LIM3 LIM4 PY PY 31 118 PYPY 188 190 Crk Src adaptor protein tyrosine kinase J. Biol. Chem., 274: 9847-9853 (1999) etc.
A ( - ) ( + Leptomycin B 2 hr) B NLS LD LD LD LD LIM LIM LIM LIM Hic-5 CKPRSPKPVAPVAPPFSSSSGVLGNGLC ELD RLLQ CSEIWDR I TTHPKYS DIDV DGLCSELMAKAKC YAP1 CPKVWSK I INHPRFE SFEI DDLCSKLKNKAKC pap1 LSSLLDDM LTSMLADL LDRLMASLSDFRV HIV Rev-type consensus hyx 1~4 hyx 2~3 LXhy Zyxin Trip6 Hic-5 Mol. Biol. Cell. 14: 1158-1171 (2003)
Focal adhesion LIM protein Hic-5 The focal adhesion protein, Hic-5: its role as a molecular adaptor shuttling between focal adhesions and the nucleus Motoko Shibanuma Abstract Hic-5 is a multidomain LIM protein providing a molecular scaffold for various cellular activities, including integrin signaling at focal adhesions and transcriptional activities in the nucleus. Notably, Hic-5 translocates in and out of the nucleus dependent on CRM1 and potentially coordinates cytoplasmic and nuclear activities. It should be emphasized that the nuclear-to-cytoplasm export of Hic-5 is distinctive because of its sensitivity to the cellular redox state. Key WordsHic-5LIMcell focal adhesionreactive oxygen species, nuclear export signal