(pathogens) (contamination) 1. 2. 3. 70% 2%(Lysol) 4. 5. 6. 7. 8. 9. (loop)(needle)(pipette)
10. 11. 12. 13.
Chapter 1 1 2 3 4 5 6 7 3
1 (Bacteria in Environment) 1 (microorganism) (colony) 1. 2. 3. 1. (nutrient agar plate) (1) (beef extract, 1%) (2) (peptone, 1%) (3) (NaCl, 0.3~0.5%) (4) (agar, 1.5%) 2. 1. (1) (2) 30 (3) 4
2. (1) (2) (3) 3. (1) (W; N) (2) N Z (3) (4) (2) W Z (5) 4. 5. (agar side up) 37 C 18~24 1 1. 2. 3. 1. 2. Chapter 1 1 5
Microbiology-Selected Experiments 1 6
1. (1) 1 (2) (3) (N) (W) (4) Chapter 1 1 7
Microbiology-Selected Experiments 2. 1 1. 2. 3. 37 C 8
2 (Inoculation of Bacteria) (isolation and pure culture) 2 1. 2. 1. (agar)solid medium 1.5% semi-solid medium 0.65~1% liquid medium broth 2. (petri dish) (plate)(slant) (1) (isolation)(pure culture) (colony count)(pour plate) (streaking method)(loop) Z 2-1(a) I II 2-1(b) Chapter 1 2 9
Microbiology-Selected Experiments III IV 2-1(c)(d) 90 2 2-1 (2) 2-2(a) 2-2(b) 2-2 3. (stabbing method) (needle) 2-3 10
2 2-3 4. (stirring) 5. (Staphylococcus aureus)s (Escherichia coli)e 1. (1) (2) (3) (4) 2. 37 C 1. 2. Chapter 1 2 11
Microbiology-Selected Experiments 2 3. 30~300 4. 5. 12
1. 2 2. Staphylococcus aureus Escherichia coil 3. (1) (2) Chapter 1 2 13
Microbiology-Selected Experiments (3) (4) 2 1. 2. 14
3 (Staining Methods) 1. (Simple stain) 3 2. (Differential stain) (Gram stain)(acid-fast stain) 3. (Special stain) (flagella stain)(capsule stain)(spore stain) (nuclear stain) 4. (Negative stain)(acidic dye)india inknegrosin (background)(spirilla) 3-1 (Simple Stain) (morphological shapes)(arrangement) Chapter 1 3 15
Microbiology-Selected Experiments (methyl blue) (methylene blue)(crystal violet) (carbolfuchsin)(positive charge)(basic dye) (nucleic acid)(negative charge) 3 1. nutrient agar plate broth Escherichia colistaphylococcus aureus 24 (young culture) 2. methyl blue methylene blue(cedarwood oil)(xylene) 3. (loop)(microscope)(glass slide) 1. 2~3 2. (liquid medium)(solid medium) (1) 1 (2) 3. 2~3 16
4. 1~2 1~3 5. 6. 7. 100x (oil immersion) 8. 100x 3-2 (Gram Stain) 3 (crystal violet)(primary stain) (mordant)(gram s iodine solution) (complex) 95%(95% alcohol)(decolorizing agent) (safranin) (counter stain) 1. nutrient agar plate broth Escherichia colistaphylococcus aureus 24 Chapter 1 3 17
Microbiology-Selected Experiments 2. crystal violet Gram s iodine 95% alcohol safranin O carbolfuchsincedarwood oilxylene 3. 3 1. 3-1 2. Escherichia coli Staphylococcus aureus 3. 4. 1~2 crystal violet 1 5. 6. 1~2 Gram s iodine 1 7. 8. 95% crystal violet 9. 10. safranin O 30~45 11. 12. 13. 100x 18
3-1 1. 3 2. Escherichia coli Staphylococcus aureus Chapter 1 3 19
Microbiology-Selected Experiments 3-2 3 1. 2. 3. 4. Escherichia coli Staphylococcus aureus 20