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Analytik Jena AG 2007-2 -

1... 5 1.1... 5 1.2... 6 1.3... 6 1.3.1... 6 1.3.2... 7 1.3.3... 7 1.4... 8 1.4.1 Lambert-Beer... 8 1.4.2... 8 1.4.3... 9 1.5... 9 1.6 UV VIS... 10 1.6.1 DNA... 10 1.6.2 DNA... 11 1.7... 12 1.7.1... 13 1.7.2... 14 2 UV VIS... 16 2.1 UV VIS... 16 2.2... 16 2.3... 16 2.3.1... 16 2.3.2... 17 2.4... 18 2.5... 19 2.6... 19 2.7 /... 19 3... 20 4 UV VIS... 23 4.1... 23 4.1.1... 24 4.1.2 DNA... 24 4.2... 25 4.2.1... 25 4.3... 27 4.3.1... 27 4.4... 29 4.4.1... 29 4.4.2 SPECORD... 29 4.4.3... 30 4.4.4... 32-3 -

4.5... 33 4.5.1 ATR... 33-4 -

1 1.1 UV VIS 1 () 2() UV VIS - 5 -

1.2 I 0 d () I 3 T 1 A 2 1.3 1.3.1 UV VIS UV VIS ( ) 1 1 -( ) RR C=O 271nm -( ) RHC=O 293nm - RCOOH 204nm - RCONH 2 208nm - -N=N- 339nm - -NO 2 280nm 1,5-185nm 1,3,5-250nm - 6 -

UV VIS 4 UV VIS 1.3.2 ( )( ) 2 nm n- 195 240 200 200 185 ph 1.3.3 UV VIS HPLC DNA/RNA - 7 -

1.4 1.4.1 Lambert-Beer Bouguer-Lambert-Beer Bouguer 1696 1758 Lambert 1728 1777 Beer 1825 1863 3 A( - ( - L mol -1 cm -1 c - mol L -1 d - cm Lambert-Beer > 0.01M/I) ( ) Lambert-Beer 1.4.2 () Lambert-Beer 5-8 -

1.4.3 7500 3 UV VIS 3 BSB CSB TOC 1.5 6-9 -

7 4 4 ( ) C 1.6 UV VIS 1.6.1 DNA DNA (Pu-Py) (A-T) (G-C) DNA UV 260 nm 260 nm Lambert-Beer 8 DNA 260nm 280nm - 10 -

DNA 260 nm 280nm 1.8 2 70% - 95% 280nm () Lambert-Beer 280nm Bradford G-250 595nm Cu 2+ 546 nm Lowry 540-750 nm ) 1.6.2 DNA DNA Tm 260 nm - 0.1 9 DNA - 11 -

10 DNA 260nm Tm GC DNA AT DNA Tm 57.7 Tm DNA 1.7 UV VIS Lambert-Beer 3 1 4-12 -

2 5 3 6 4 7 - - 1.7.1 11-13 -

[nm] 380 435 435 480 480 490 490 560 560 595 595 650 650-780 CIE XYZ * DIN 5033 ASTM E-308 * CIE XYZ de I'eclairage 12 1.7.2 1 2 13-14 -

8 d m n 1 2 1 > 2 nm 14 45 0 6.45 m - 15 -

2 UV ViS 2.1 UV VIS UV VIS (AD) 2.2 UV VIS UV VIS 15 2.3 2.3.1-16 -

16 SPECORD 40 2.3.2-17 -

17 SPECORD S 600 2.4 266 269 nm 18-18 -

5 A 266nm A 269nm A/A 4nm 0.3252 0.3265 1.0 2nm 0.2784 0.4026 1.4 1nm 0.2384 0.4823 2.0 0.5nm 02244 0.5229 2.3 0.5 nma/a 4 nm 2.5 2.6 UV VIS PMT (190-1100 nm) - - - 0.5 A/W 10 4 10 5 10x10 mm 2 10 6 A/W 10 6 50mm 2 1200 V 180-650 nm, 180-900 nm 100 1000 200 2. 7 / SBT - 19 -

3 19 20-20 -

22-21 -

PL 10mm 750PL 10mm 450PL 10mm 80 PL 50mm 3750 PL 50mm 2250 PL 50mm 370 23 0.01 0.5mm - 22 -

4 4.1 100mm 37 PCT - 23 -

4.1.1-2- Carrez Spectroquant MerckNo. 1.14815.0001 Bioquant Carrez MerckNo. 1.10537.0001 1 mol/l MerckNo. 1.09137.0001 Neutralit ph 5-10 MerckNo. 1.09533.0001 Oxisolv MerckNo. 1.12936.0030 Merck Art. 1.14613.0001 MW 500 Merck Cat. No. 1.14872.0001 GR Merck Cat. No. 1.16754.9010 5 ml Carrez-1 Carrez-2 5 ml 1 mol/l ph 7.5-8.0Neutralit ph 5-10 250 ml 10 ml 500 mg (= 5 ) Oxisolv 500 65 s 5 550 nm mg/l = mg/l x 50 4.1.2 DNA DNA 260 nm 1 cm 500 - DNA ds33 - DNA ss 40 RNA [ng/ l] = A (260) * 500 ds DNA 6-24 -

6 [ng/ l] [ng/ l] 0.007 3.50 0.50 0.017 8.50 1.00 0.033 16.67 0.29 0.074 37.17 2.02 0.150 75.17 1.76 0.290 145.17 2.36 0.601 300.33 7.18 1.161 580.50 9.64 2.154 1076.83 13.29 4.2 APG 4.2.1 1 2 5cm - 25 -

24 1 40g 1L 2 2.6 g L(+) 150 ml 3 2.7 g (111) 1L 4 25 H 2 SO 4 250 ml 4 75 ml 1 150 ml 2 25 ml 3 2 40ml 50 ml 8 ml 50 ml 10 700 nm. 320nm 700nm [s] 0.2 [s] 30-26 -

4.3 6-1, 2, 5 cm 15 1cm 8-2 8 50-50 1cm 4.3.1 25-27 -

- R 2 5 30 1 0.0001 0.9994 0.0001 1 5 30 1 0.0003 0.9994 0.0003 2 5 30 1 0.0015 0.9988 0.0015 3 5 30 1 0.0026 0.9984 0.0026 26-28 -

4.4 4.1.1 4.4.2 SPECORD [nm] 350 700 [nm] 0.2 320nm [s] 0.2 0.5nm 535 nm 530~700 nm - 29 -

27 25 ) 28 25 ) m 27 26 1 [nm] 535.0 698.4 2 [nm] 535.2 690.4 n 1.5 1.5 0 0 d[ m] 20.6 20.6 4.4.3 Ulbricht DIN 6167-30 -

SPECORD 380 780nm 0.10s 1nm 10nm/s 4nm A B C 29 Analytik Jena AG 10 o D65 ASTM E 313-67= 4*B-3*Y = 100* 1-B/YB=0.8475*Z Y Z CIE 7 A 46.53 14.13 B 31.66-2.25 C 41.62-11.36-31 -

4.4.4 UVA UVA 2604:98 320~360nm 90% UVA 2004 DIN 67502 UVA UVA UVA UVA SPF UV 8 8 SPF UV 1 6 octyl methoxycinnamate phenylbenzimidazole sulphonic acid 2 12 ethylhexyl methoxycinnamate octocrylene buthyl methoxydibenzoylmethane 3 15 ethylhexyl methoxycinnamate octocrylene buthyl methoxydibenzoylmethane 4 15 ethylhexyl methoxycinnamate octocrylene buthyl methoxydibenzoylmethane 5 20 ethylhexyl methoxycinnamate octocrylene buthyl methoxydibenzoylmethane 6 30 octocrylene ethylhexyl methoxycinnarnate phenylbenzimidazole sulphonic acid UVB UVA 1 UVB SPECORD Ulbricht 75 mm Spectralon Spectralon 0.75 mg/cm 2 1-32 -

15 SPECORD 290 400 nm 30 SPF 15 3 320~360nm 60% SPF 6 1 1 2604 UVA UVA UVA SPF UVA SPF SPF 3 4 UVA DIN 67502 4.5 4.5.1 ATR ATR ATR ATR ATR ATR SPECORD SPECORD 370~800 nm - 33 -

31 528nm 562nm 446nm 600nm 638nm Analytik Jena AG CIELAB 10 o D65 x, y, z Cartesian CIELAB L 'a'-'b'- CIELAB X Y Z L a b 81.36 74.23 99.95 89.03 22.42-13.98 83.35 83.35 36.36 93.17 8.44 48.80 63.32 72.27 108.81 88.10-11.65-21.43-34 -