1 0 3 8 4 9726023 UDC X MGC80-3 2000 7 2000 7 2000 7
2000 6 22.3.4...11..11 12 13 15 15 20 20 X MGC80-3. 22 X 22 X 23 X MGC80-3.23 X BALB/c 26 X MGC80-3.27 X MGC80-3 28 29 29 30 31 32 33 X MGC80-3 34 X..34.35.36 Abstract.43 44
MTT TRAP DNA X MGC80-3 10 ng/ml X MGC80-3 4 50% 7 84% MGC80-3 X 4 2 ng/ml 10 ng/ml X MGC80-3 X 10 ng/ml X 6 9 100% BALB/c X 0.1 mg/kg 0.35 mg/kg 7 MGC80-3 BALB/c X 10 ng/ml 0.3 mg/kg X X MGC80-3 1 100% 2 3
4 5 replicative senescence 1938 1985 [1] RNA RNA 11 5 CUAACCCUAAC -3 5DNA [2] [3] TP1 2627 hest2p [4] DNA G DNA TTAGGG n 15Kb DNA DNA, [5] htrf [6][7] 1 1 G C G DNA C Blackburn binding elongation [8] translocation DNA RNA [9]
Sun 2 6 IC50 23 [10] G- Burger G-Pt-G [11] Carbocynamine [10] 1 2 telomere binding proteins TBPs [5] TBPs TBPs TBPs 2 1 RNA Yoshiyuki RNA TeloRZ a hammered Ribozyme TeloRZ RNA TeloRZ HepG2 Huh-7 TeloRZ [12] RNA Feng htr human telomerase RNA HeLa 23-26 33 [13] 28 Norton RNA PS PNA PNA IC50 p mol/l ~ n mol/l [14] PNA Pitts [14] 2 O RNA PNA [15] 2 2 Stahl ddg AZT [16] Yegorov AZT Carbovir AZT Carbovir [17] 2 3 Fletcher 7 dgtp 7 datp 7 dgtp 7 datp 11 8 [18] 293 50% 2 4 DNA
DNA 5 DNA [19] 2 5 2 5 1 2A Li PMC42 2A PP2A [20] 2 5 2 PKC Ku NPC-076 TOP DNA 2 PKC bisindoplylmaleimide [22] p53 p21 [23][24] [25] Bcl-2 [26][27] T TCR/TC3 [28][29] CD28 IL-2 [20][21][30] cyclind1 cycline [31] [32] Sharma DMSO D HL-60 K-562 G1 [33] [34] Albanell Bcl 2 Bcl 2 MandalKumar Bcl 2 [25] Bcl 2 necrosis apoptosis
[35][36][37] [38] 30mg/kg/d 35 80 8 [39][40] [41] [42][43]
1 RMPI 1640 Gibco 56, EDTA D-Hanks DMSO Sigma X 2 A 2-3 RMPI 1640 + 10 % +100 IU/ml +100 mg/ml +80 IU/ml 0.2~0.3ml/cm 2, 0.25% Verson 0.02%EDTA D-Hanks EDTA ),
= 100% ) ) ) 10 mmol/l HEPEs-KOH (ph 7.5), 1.5 mmol/l MgCl 2, 10 ng/ml mmol/l KCl 1 mmol/l DTT
0.2mg 1 10 5~7 1~2, 10 ng/ml mmol/l Tris-HCl ( ph 7.5 ), 1 mmol/l MgCl 2, 1 mmol/l EGTA, 0.1 mmol/l PMSF, 5 mmol/l, 0.5% CHAPS, 10% 100~200 l (200~300 rpm) 1-2, 30 4 14000 rpm 15 C Bio-Rad BSA 1.4mg/ml 0 1 2 3 5 7 10l 800 l 200 l A 5 Beckman 595nm OD 1~2l 800l ( 2~3 D [51] PCR 20 mmol/l Tris-HCl ( ph 8.3 ), 1.5 mmol/l MgCl 2, 63 mmol/l KCl, 0.005% Tween-20, 1 mmol/l EGTA, 50 mol/l dntps, 0.2 0.4 l - 32 PdATP (10 Ci/ L, 3000 Ci/mmol), 50 pmol TS (5'-AATCCGTCGAGCAGAGTT-3'), 2U Taq DNA, 6 g 50 pmol CX-ext (5'- GTGCCCTTACCCTTACCCTTACCCTAA -3') CX-ext 25 30 TS 90 2 94 30 50 30 72 1.5 31 PCR PCR 12% 10 V/cm 4~5 0.5M NaCl 50%40mmol/L ph4.2 30 Kodak X D-19b X F-5 3 MGC80-3 10 ng/ml X,
9 Johnson Matthey PBS Sigma 618 2 4 6 Sigma PBS 4
5'- CCCTAACCCTAACCCTAA -3' 30 pmol 10 B
5'- CCCTAACCCTAACCCTAA -3' 1 pmol/ml 1 MTT (A) C
2 MGC80 3 X mg/ml (ng/ml) () (%) 5 10 20 30 50 100 4(%) 16 50 54 54 54 52 7 (%) 20 84 96 98 97 100 c
X MGC80-3 1 X MGC80-3 1 2 3 2ng/ml X 3 4 2ng/ml X 6 5 2ng/ml X 9 6 10ng/ml X 3 7 10ng/ml X 6 8 10ng/ml X 9 MGC80-3 6bp MGC80-3
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