WinMDI WinMDI 2 Region Gate Marker Quadrant Excel
FACScan IBM-PC MO WinMDI WinMDI IBM-PC Dr. Joseph Trotter the Scripps Research Institute WinMDI HP PC WinMDI WinMDI PC MS WORD, PowerPoint, Excel, LOTUS 123 : Windows Multiple Document Interface For Flow Cytometry, Version 2.8 : http://facs.scripps.edu/software.html 1.1 : FACS101 WinMDI2.8 Setup 1. WinMDI 2.8 Installation Next 2. Software License Agreement Yes 3. Information Next 4. User Information Next 5. Installation Directory Next WinMDI28.doc 1 02/05/20
6. Setup Type Typical 7. Select Program Folder Next 8. Setup Complete Finish 9. WinMDI28.doc 2 02/05/20
2. WinMDI:/Datafile C:/ D:/ NORM001-012 WinMDI NORM001-4 NORM005-8 NORM009-12 SimulTEST CD CD CD CD : FITC PE NORM001 IgG1 Isotype Control IgG2 Isotype Control NORM002 CD3 CD19 NORM003 CD3 CD4 NORM004 CD3 CD8 2.1 WinMDI WinMDI ICONs WinMDI 2.8 WinMDI WinMDI WinMDI 2.8 2.2 WinMDI Display 1. WinMDI Display Dotplot OK WinMDI28.doc 3 02/05/20
2. C:/Datafile norm001 OK 3. FSC-H 256 FSC-H X- SSC-H 256 SSC-H Y- Plot Number of Events 3/4 (4500) Color 2D Region OK norm001 2.2.1 (Region) 1. Tools Regions Tool SortRect Creat ( Ellipse Polygon ) WinMDI28.doc 4 02/05/20
2. OK 2.2.2 (Gate) 1. Display Dotplot norm001 OK 2. FL1-H 256 FL1-H X FL2-H 256 FL2 Y Plot Number of Events All (6000) Color 2D Region 3. Gates Gate List And [ ] OK 4. Format Dotplot OK Norm001 FL1-H / FL2-H 2.2.3 1. Tools Quadrant Tool OK 2. WinMDI28.doc 5 02/05/20
3. FL1-H / FL2-H pop-up Stats 2.2.4 (Print Page) 1. File Format Print Page 2. FSC/ SSC active Edit Copy 3. active Edit Paste WinMDI28.doc 6 02/05/20
4. FL1-H / FL2-H active pop-up Copy 5. active Edit Paste 6. active File Print Active 2.2.5 1. FSC-H / SSC-H active pop-up Next Norm002 2. FL1-H / FL2-H Norm002 3. Tools Set File Increment File Increment 2 OK 4. Window Increment All Norm004 5. FL1-H / FL2-H WinMDI28.doc 7 02/05/20
2.3 2.3.1 1. Display Histogram Norm001 OK 2. Select Parameter FL2-H 256 FL2-H 3. Gates And [ ] OK 4. Read 2.3.2 1. FL2-H active 2. Tools Marker Tool 3. 4. Marker Tool Marker Index 2 Marker OK 5. FL2-H active pop-up Stats marker WinMDI28.doc 8 02/05/20
6. File Preference Short Stat Text OK File Name Events %Total %Gated Markers Marker CV Geometric Mean Median 7. Stats-Histo Format Edit Statistics OK 8. Stats-Histo Save As test.txt OK 2.3.3 1. Tools Set File Increment 1 OK WinMDI28.doc 9 02/05/20
2. Window Increment All Norm002 3. Stats-Histo test.txt Append Norm002 Microsoft EXCEL test.txt 2.3.4 1. FL2-H active 2. Window Open File 3. Norm001 FL2-H 256 FL2-H Overlay ( Read) FL2-H FL2-H 4. Display Format Format Overlay 2.4 1. 2. File Exit Yes. WinMDI28.doc 10 02/05/20
3. 3.1 File Menu Open File Next File Prev File Gate File Save As List... Export Data Print Active Print Screen Format Print Page Record Preferences Exit 3.2 Edit Menu Undo Cut Copy Paste Delete Select All Copy Graphics Format Empty Clipboard View Clipboard 3.3 Display Menu Histogram Dotplot Densityplot Contour Format Retain Markers Screen Stats Edit Stats WinMDI28.doc 11 02/05/20
3.5 Tools Menu Markers, Regions, Quadrants, Logical Gate Marker Tool Regions Tool Quadrant Tool Logical Gate Tool Batch File Tool Annotate Parameter Labels Set Standard Logical Gates Sort Windows Set File Increment Set Font Free Memory 3.6 Window Menu Copy Active Tile Cascade Minimize All Restore All Arrange Icons Close All Increment All Decrement All Fixup Fixup All Refresh Refresh All Set Defaults 3.7 Help Menu HELP MENU WinMDI28.doc 12 02/05/20