Microsoft Word 唐红梅-二校出

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1 7 10 Vol. 7 o Journal of Food Safety and Quality ct., 2016 唐红梅 1, 曾芳 2, 李成洪 1, 3* (1., ; 2., ; 3., ) 摘要 :,,,,,,,,, 关键词 : ; ; Progress on the detection of nitrofurans drugs residues and their metabolites in food TAG Hong-Mei 1, ZEG Fang 2 1, 3*, LI Cheng-Hong (1. Chongqing Academy of Animal Sciences, Chongqing , China; 2. Chongqing University, Chongqing , China; 3. Chongqing Municipal Key Laboratory of Pig Science, Chongqing , China) ABSTRACT: itrofuran drugs are a kind of synthetic broad-spectrum antibiotics, including furazolidone, nitrofurantoin, nitrofural and furaltadone. Due to the irrelatively cheap price, they are widely applied in the prevention and treatment of bacterial diseases in poultry, livestock and aquatic products. In vivo metabolize of the original drugs is fast and half-life is short, in addition, their metabolites combine proteins tightly and remain long time in the body. The study revealed that nitrofuran drugs and metabolites had potential side effects such as carcinogenic and teratogenic, which leaded this kind of drugs were totally banned from using in the process of animal food production in the whole world, as well as testing standards had been developed, however, nitrofuran drugs were often illegally used by animal farmers. There are lots of methods for the detection of nitrofuran drugs, like chromatography and immunology. Each method has its merits and drawbacks. This paper summarized the detection methods for residues of nitrofurans drugs and their metabolites in food in recent years, and discussed the trend of the detection methods for the metabolites of nitrofuran drugs in the future. 基金项目 : (16411) Fund: Supported by Chongqing City Agricultural Development Fund Project (16411) * 通讯作者 :,, lchenghong@126.com *Corresponding author: LI Cheng-Hong, Senior Veterinarian, Chongqing Academy of Animal Sciences & Chongqing Municipal Key Laboratory of Pig Science. o. 51, Chang Long Road, Chang State Street, Rongchang District, Chongqing , China. lchenghong@126.com

2 10, : 3953 KEY WRDS: nitrofuran drugs; metabolites; residue analysis 1 引言, [1] 20 60,, ( ),, 5 ( 1), [2], [3] (furazolidone) (nitrofurantion) (nitrofurazone) (furaltadone) [4] ( 1), [5],,, [6], [7],,, [8,9] (3-amina-2-oxazolldinone, AZ) (3 - amino methyl oxazole morpholine generation ketone of alkanes, AMZ) 1- - (1-Aminohydantoin, AHD) (semicarbazide, SEM),, [10,11], [12] 2 H 2 呋喃唑酮 3- 氨基 -2- 恶唑烷酮 2 呋喃妥因 H H 2 H 1- 氨基 - 乙内酰脲 2 H 2 H H 2 H 2 H 呋喃西林氨基脲 2 H 2 呋喃它酮 3- 氨基 -5- 吗啉代甲基 -2- 恶唑烷酮 Fig Four kinds of drug and metabolites of nitrofuran

3 3954 7,, [13],, [14,15] 1995,, 1997 A, 2002, /181/EC, (minimum required performancelimits, MRPL) 1.0 μg/kg [16] ,, , 193,, ( ), 2004,, - [17,18],, [19],,,, 2 硝基呋喃类药物及其代谢物检测方法技术研究进展 2.1 色谱分析方法, [20] 高效液相色谱法 HPLC 30,,, HPLC , HPLC 1997, Angelini [21] HPLC, 1.0 mg/kg, 2.0 mg/kg 2003, [22] HPLC 4, 2.0~5.0 μg/kg ; 2005 [23] HPLC AZ, 1.0 μg/kg,, HPLC 2010, [24] HPLC,,,,, 0.2~0.12 mg/kg, 0.999, 70%, 2013 Sheng [25], - (high performance liquid chromatographyfluorescent detection, HPLC-FLD) 4, C 18, (ph 10.3, )-, 0.999, 92%, SEM 2.0 μg/kg, 1.0 μg/kg, LC-MS/MS,, SEM,,, 2014 [26] - 4, 10% -,,, 0.05 mol/l - (V:V=30:70) SEM 0.75 μg/kg, SEM,,, 液相色谱 - 质谱法, -, LC-MS,, LC-MS,, - [27] 75~201 g/mol, LC-MS,,,, [28] 2- (2-BA), Pereira [29]

4 10, : BA, 0.5 μg/kg, 2-BA Leitner [28] ( -3-2, ), [30] 2-2-BA 4,, [31,32] 2012, Douny [33] 4, (ES + ) (MRM), LC-MS/MS ~0.36 μg/kg, MRPL, AZ AMZ AHD 3, SEM 2015, [34] - (high-performance liquid chromatographytandem mass spectrometry, HPLC-MS/MS) ~0.30 μg/kg, 4, 2016, [35] -, 0.5 μg/kg,, ( ), SEM, SEM 超高效液相色谱 - 串联质谱法 (ultra performance liquid chromatography, UPLC) HPLC, UPLC HPLC , ( ),,,,, 2013,, [36] (UPLC-MS/MS) ,,,, 2014 [37] SEM AHD 0.1 μg/kg, AMZ AZ 0.05 μg/kg,,,,, 2015, Kaufmann [38] - (ultra performance liquid chromatography-high resolution mass spectrum, UPLC-HRMS) μg/kg,,, 2015, [39] μg/kg,,,,, 2.3 免疫分析法 ( ),,, (ELISA),,, 酶联免疫吸附法, 2013, Chadseesuwan [40] 1- (1-amino ethyl lactam, AHD)

5 3956 7, 0.11 μg/kg,, LC-MS/MS,, Shen [41] 4, AZ AMZ AHD SCE μg/kg, LC-MS/MS,,, ELISA,, AHD 3,, 2014, Wang [42] (dcclelisa) (icclelisa) (AHD),, AHD μg/kg,, AHD ELISA, 2014, Shu [43] ELISA (AMZ) ; 2015, Le [44] ELISA (AZ), LC-MS/MS,, ELISA LC-MS/MS 免疫层析法 (colloidal gold immunochromatography, GICA) (immunochromatography, ICA),,,, [45] 2012, [46], (AMZ), 1.0 μg/kg,, 5%, 0, AMZ 2013, [47] 4, AZ 0.5 μg/kg, μg/kg, LC-MS/MS, 98%, GICA, GICA, 2014, Li [48] (AMZ), 0.1 μg/kg, 2015, [49],, (AZ), μg/kg, AZ, 2.4 其他方法,,,, μg/ml,, [50], 440 nm, 0~0.1 mg/ml, 0.999, 95%~102%,,,,,, Abjean [51], 5 μg/kg 3 问题与展望,,,,, 4,,,,,,,,,,,,

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