夜光蟲概況

Size: px

Start display at page:

Download "夜光蟲概況"

雉蹄焦
4 years ago
Views:

1 -

2 - (luciferin-luciferase)

3 วӀᐒ ڋ 儻兟䴇㵞垉䙣ℰ䴇 Ϲ

5 (ostracod) (Noctiluca scintillans) (luciferase)(luciferin binding protein)

8 2-2 μm (.2-2 cm) ()

Kingdom Chromista Subkingdom Harosa Infrakingdom Alveolata Phylum Myzozoa Class Dinophyceae () Order Noctilucales Family Noctilucaceae Genus Noctiluca Species N.

9 Kingdom Chromista Subkingdom Harosa Infrakingdom Alveolata Phylum Myzozoa Class Dinophyceae () Order Noctilucales Family Noctilucaceae Genus Noctiluca Species N. scintillans (Macartney) Kofoid and Swezy 1921 From WoRMS (World Register of Marine Spceies) D E C Chromalveolates B Unikonts A F Ald et al. 25; Baldauf

Jul. 216, M7_In 2-2 μm, n = 354 reads Noctiluca

Protoceratium_reticulatum Alexandrium_catenella

10 Jul. 216, M7_In 2-2 μm, n = 354 reads Noctiluca 93.2% Noctiluca_scintillans Protoceratium_reticulatum Alexandrium_catenella Ceratocorys_horrida Alexandrium_tamarense Ceratium_fusus Alexandrium_monilatum Gonyaulax_spinifera Pyrodinium_bahamense Tiarina_fusus Karenia_brevis Pyrocystis_fusiformis Gonyaulax_polyedra (Sheng-Fang Tsai) Jul. 216, M8 2-2 μm, n = 818 reads Noctiluca 97.2% Jul. 216, M7_In > 2 μm, n = 654 reads (Tomas, 1997) Jul. 216, M8 > 2 μm, n = 335 reads M7_In Noctiluca 96.9% Noctiluca 85.1% M8 (Pedinomonas noctilucae) From Harrison et al., 211 From Furuya et al., 26

11 22

12 ph DNA

35 25 Temperature ( o C) 3 25 2 15 1 5 J F M A M J J A S O N

F M A M J J A S O N D 216 217 218 219 Month Month 4 35 3

13 35 25 Temperature ( o C) J F M A M J J A S O N D Noctiluca scintillans (cells/l) J F M A M J J A S O N D Month Month Salinity J F M A M J J A S O N D Month

14 12 8 NO 3 (μm) SiO 4 (μm) J F M A M J J A S O N D J F M A M J J A S O N D Month Month 6 5 Nutrient limitation PO 4 (μm) J F M A M J J A S O N D Month DIN 1, P.2, Si 2 (Dortch and Whitledge, 1992) Si limitation ( ) P 217 > 218 > 216 Total Chl a (mg/m 3 ) J F M A M J J A S O N D DO (%) J F M A M J J A S O N D Month Month 14 > 2 μm Chl a (mg/m 3 ) J F M A M J J A S O N D Month >2 μmchla (~8%) DO (%) 217 > 216, 218 >2 μmchla 217 > 218 > 216

15 Noctiluca scintillans (cells/l) Temperature ( o C)

16 Tsia S-F*, L-Y Wua, W-C Chou and K-P Chiang 218 ()The dynamics of a dominant dinoflagellate, Noctiluca scintillans, in the subtropical coastal waters of the Matsu archipelago, Marine Pollution Bulletin 127: (IF=3.146, Marine & Fresh Water Biology: 8/15=8%, : )

17 33 34

35 Noctiluca scintillans (cells/5ml) 5 45

18 35 Noctiluca scintillans (cells/5ml) /4/1 218/4/21 218/5/11 218/5/31 218/6/2 218/7/1 218/7/3 Date pair-sample T test v.s. v.s

19 Temperature ( o C) J F M A M J J A S O N D 3 Month 25 Salinity J F M 14 A M J J A S O N D 12 Month J F M A M J J A S O N D Month DO (%) PO 4 (μm) J F M A M J J A S O N D Month NO 3 (μm) SiO 4 (μm) J F M A M J J A S O N D Month J F M A M J J A S O N D -1 Month Noctiluca scintillans (cells/l) Temperature ( o C) Noctiluca scintillans (cells/l) Salinity

20 㼙㽕㘩㘖㕟憶廪檿烎㗆㵩ⵤ (ɗ) 嚰 (暢/L) 匇䱞䯞 (mg/m3) 匇䱞䯞烋 ĲıġƶŮġ(mg/m3) 点㕟憶ˣ㽫䙭䂡䨴 (Cells m -3)ƍ 活ⵤ 匇䱞䯞烍 ĲıġƶŮġ(mg/m3)

22 - (luciferin-luciferase)

23 () ( ) () )

24 Paralytic shellfish poisoning, PSP :dinoflagellate Neurotoxic shellfish poisoning, NSP :dinoflagellate Diarrhetic shellfish poisoning, DSP :dinoflagellate Amnesic shellfish poisoning, ASP :diatom Ciguratera fish poisoning CFP

25 NSPbrevetoxin-A, brevetoxin-b 3 voltaged-gated sodium channel activator DSPokadaic acid dinophysistoxins protein phosphatase 1 2a PP1 PP2a 3

26 ASP brown tidedomoic acid DA glutamate synthetase () Gambierdiscus toxicus Cigua: Ciguatera was first described by one of the surgeon's mates, William Anderson, on the crew of HMS Resolution in 1774 Ciguatoxin is odourless, tasteless and very heatresistant, so ciguatoxin-laden fish cannot be detoxified by conventional cooking Symptom: gastrointestinal () and neurological ( ) effects: Severe cases of ciguatera can also result in cold allodynia (), which is a burning sensation on contact with cold The symptoms can last from weeks to years, and in extreme cases as long as 2 years, often leading to long-term disability. CIGUATERA)

27 French Polynesia Tahiti Gambier Islands

医学实验动物的特点及应用

医学实验动物的特点及应用 Miniature Swine Taxonomy Kingdom Animalia Phylum Chordata Class Mammalia Order Artiodactyla Family Suidae Genus Sus Species Sus scrofa domesticus , 3.1.4.3/ 3.1.4.3 :,,., 0.0.3.3/ 3.1.3.3 1980 80 3R