,2018,38(10): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2018) 犱狅犻 : /j.issn "#$4% 犘犗犇 &' (,,,,,,! ( 7

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$,@ A RT PCR B C D E 4 犘犗犇 ', = 犌犼犘犗犇 4 犌犼犘犗犇 5 犌犼犘犗犇 7F 犌犼犘犗犇 42(GenBankGHI = MH708528 MH708531 MH708529F MH708530) JK4 ' LM, N O, O SA PF <QRSSTU PV4 犘犗犇 ' WXY POD W Z[\,=]^ < 犘犗犇 ' W_` a bc '$

1 ,2018,38(10): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2018) 犱狅犻 : /j.issn "#$4% 犘犗犇 &' (,,,,,,! ( 78,92$+:,78;, ) ) *: < = >?,@ A RT PCR B C D E 4 犘犗犇 ', = 犌犼犘犗犇 4 犌犼犘犗犇 5 犌犼犘犗犇 7F 犌犼犘犗犇 42(GenBankGHI = MH MH MH708529F MH708530) JK4 ' LM, N O, O SA PF <QRSSTU PV4 犘犗犇 ' WXY POD W Z[\,=]^ < 犘犗犇 ' W_` a bc ' ^:(1):BCW4 犘犗犇 ' WcDNA =966~1005bp, 321~334 ' ;, N O, W Ⅲ POD, Motif, I F Z (2)LZ O X,GjPOD4F GjPOD7 AtPRX52 GjPOD5 AtPRX47 GjPOD42 AtPRX42 (3) SA P F < Q R S S T U 4 ' W X Y POD, 犌犼犘犗犇 4 3 犌犼犘犗犇 ' WXY W (4) O ^,, 犌犼犘犗犇 42W XY POD ( 犘 <0.05); V,4 犘犗犇 ' X Y POD $+X,:BCW 4 犘犗犇 ' < a 2, 2. A, 犌犼犘犗犇 4 3 犌犼犘犗犇 W_` +,-: Z ; <;' XY; ;a./ 0:Q785;Q786;Q789 1 :A 犆犾狅狀犻狀犵犪狀犱犈狓狆狉犲狊犻狅狀犃狀犪犾狔狊犻狊狅犳犉狅狌狉犌犲狉犫犲狉犪犘犲狉狅狓犻犱犪狊犲犌犲狀犲狊 HAO Xiangyang,SUN Xueli,LIU Fan,XIANG Leilei,WANG Tianchi, LAIZhongxiong,CHENGChunzhen (InstituteofHorticulturalBiotechnology,ColegeofHorticulture,FujianAgricultureandForestryUniversity,Fuzhou350002, China) 犃犫狊狋狉犪犮狋 :Inthisstudy,wesuccessfulyclonedfour 犘犗犇 genes,namedas 犌犼犘犗犇 4, 犌犼犘犗犇 5, 犌犼犘犗犇 7 and 犌犼犘犗犇 42 (GenBankaccessionnumbersare MH708528,MH708531,MH708529and MH708530), from 犌犲狉犫犲狉犪犼犪犿犲狊狅狀狀犻 cv. Linglong usingrt PCR.Theircharacteristicswerethenanalyzedusingse riesofbioinformatics methods.moreover,toprovidebasisfortheunderstandingoftheirfunctionand theirfutureapplicationingerberaresistancebreeding,weinvestigatedtheirexpressionpaternstogether withpodactivitiesundersa,peg,coldstressand 犘犺狔狋狅狆犺狋犺狅狉犪犮狉狔狆狋狅犵犲犪 treatments.resultsshowed that:(1)thecodingsequencelengthofthese 犘犗犇狊 rangedfrom966bpto1005bp,encoding a minoacids.bioinformaticanalysisshowedthatalthefour 犘犗犇狊 belongtotheclass Ⅲ plantheme de pendentperoxidasesuperfamilycontainingconserveddomainsand motif,signalpeptide,transmembrane : ; : : (2015NZ0002 1); A!"# $%&' ( )!: (1993-),, ()*$+,,-./01, $+ E mail: @qq.com :234,)*,56,-./078, $+ E mail:ld0532cheng@126.com

2 structureandphosphorylationsites.(2)phylogeneticanalysisrevealedthatgjpod4,gjpod7and At PRX52,GjPOD5andAtPRX47,GjPOD42and AtPRX42sharedcloserelationship.(3)Thesegeneex pressionandpodactivitieswerealfoundtobesignificantlyorverysignificantlyinfluencedbythetreat mentsofsa,peg,coldstressandgerberarootrotpathogen.whiletheexpressionpaternof 犌犼犘犗犇 4 diferedfromother 犌犼犘犗犇狊.(4)Correlationanalysisshowedthattheexpressionof 犌犼犘犗犇 42wassignifi cantlypositivecorrelatedwithpodactivityindiferentorgans( 犘 <0.05).Notably,althegeneexpres sionweresignificantlyinducedandpositivelycorrelated withthepodactivityunderthe 犘犺狔狋狅狆犺狋犺狅狉犪犮狉狔狆狋狅犵犲犪 treatment.resultsobtainedinthisstudyindicatedthat 犌犼犘犗犇狊 playedanimportantrolein stressresponsesofgerbera,especialyduringthegerbera 犘. 犮狉狔狆狋狅犵犲犪 interaction.and,thefunctionof 犌犼犘犗犇 4mightdiferfromother 犌犼犘犗犇狊. 犓犲狔狑狅狉犱狊 :peroxidase; 犌犲犿犫犲狉犪犼犪犿犲狊狅狀狀犻 ;geneexpression;enzymeactivity;stressresistanceandde fense Z (Peroxidase,POD,EC X)! &F, W Z Class Ⅰ F Ⅲ! POD, Class Ⅲ POD(PRX,EC )=:[ [1 2] PRX ', ' 73, 138 [3 5]! POD W 'F H 2O 2 W!" [6 7],, b 2. A [8] #, POD $ % & ' [9 10] [11] [12] P [13 16] : F PV,() POD * +, -. / W [17],0 POD 1 ( ) F P W ; [18] W$+, 2 POD #$ : W, 67 8STU.,`9:;, W ROS STUW STU, W ROS <=>?@ A B 5 D E POD ` 3, as 2. A [19 20] 3 [21] W$+X 犛犮犘犗犇 02' asfc GHISUJ5, K K SFc L V X Y Zhang [22] M N 1!OW : HISU.,aS Fc 犘犗犇 ' X Y, K S F c V XY,0 犘犗犇 ' W X Y a S POD a W. A, J 犘犗犇 ' W 1 $+ < ( 犌犲狉犫犲狉犪犼犪犿犲狊狅狀犻 Bolus) P Q RS1T,U -WVWXY [23] < 犘犗犇 ' W$+ $ +,/ Z 7DEW <[H\ ]^D E4_` ab ORF W 犘犗犇 ' c,j L M1BC, N O, $$+1 P VK ' FPOD W Z[\,=]^ < 犘犗犇 ' W_`. <a bc W A ' 1 >? A < 78, 9 2 $ + : F $ + ; (1) : <Q F, A (2) :A100μmol/L (SA) < B, F24h (3)PEG :A 30% (PEG), F6h (4) : < 4,.0 4 8F12h (5)QRSU c:/28 5dW U = 0.5 mm U 100 mlpdb ',28 _ V200r/min4 2d, 3. A <Q. U 2h,, F6d Q : F. :,T. -80,A. RNA POD POD Z H 2O 2 Z [, ; 470nm [\, A,@A Z (, ) F POD

3 107, : <4 犘犗犇 ' WBC XY O 犚犖犃 9 : 犮犇犖犃 A Trizol RNA (TaKaRa) < RNA A ThermoScientificRevertAidFirstStrandcD NASynthesisKit [H 'cdna A 犘犗犇 ' cdna WBC A TransS criptal in OneFirst StrandcDNA SynthesisSu permixforqpcr (One StepgDNA Removal) ( ) [ H ' F W cdna A qrt PCR : 0 LM 犘犗犇 & 7 PCR (25μL):Dream Taq TM GreenPCR MasterMix (2 )12.5μL,ddH 2O9.5μL,cDNA1μL, V 1μL : A c X 1 PCR 2 c =94 3min,94 30s,55 ~59 30s,72 30s,30,.72 5 min A Gel/PCRExtractionKit W, pmd18 T.[Z 犇犎 5α KG,VU PCR.^ B C B ' ' LM c 犘犗犇 & <=>?@ ( A J 犘犗犇 ' LM' Z & O (ExPASy,htp://web.expasy.org/prot param/) I (SignalP4.1Server,htp:// (CELLO:S,htp://celo.life.nctu.edu. tw/) (TMpred,htp:// embnet.org/software/tmpred _form.html) Z (NetPhos3.1Server,htp://www. cbs.dtu.dk/services/netphos/) O (NCBIConserved Domain Search,htps://www. ncbi.nlm.nih.gov/structure/cdd/wrpsb.cgi) & (PSIPRED,htp://bioinf.cs. ucl.ac.uk/psipred/) / TAIR(htps://www. arabidopsis.org/) V POD c, A MEGA5.2.2 (Neighbor joining method) b "#$ 犘犗犇 & ( <18 犛狉犚犖犃 = ' L M qrt PCR cdna F,.! LM" A #$%&' 50 W cdna =, A RocheLightcycler 480 PCR L M J X Y O qrt PCR 2c:95 30s;95 10s; 1 A BCD=>? Table1 Primersusedinthisstudy Primername c Primersequence(5 3 ) W Productsize/bp Annotation GjPOD4F GjPOD4R CAACACACTCACACTCACGACT GCTCCGCCACTTATTTGAC 1142 GjPOD5F GjPOD5R GjPOD7F GjPOD7R GCCCCTCCCTTTATAAACCACT TTACTGCTGCTTCTGGCTCGG GACACACTTCTCTTGATTCCAC CACTCCAAAACCCACACC RT PCR GjPOD42F GjPOD42R CAGAGTTCCAAGCACCACAC AACACATTGCAAGCCACCAT 1117 GjPOD4 qf GjPOD4 qr ATCAGTCCCCTCACCGGAT ATGCATAGCTCCGCCACT 188 GjPOD5 qf GjPOD5 qr AGTAGCTCAGTACGCAACGA TCTCTCCTTGACCGCCTGT 105 GjPOD7 qf GjPOD7 qr ATCACTGCTAGAGACTCCGTTG GCCACCATGTCCTTAGCAGA 171 qrt PCR GjPOD42 qf GjPOD42 qr CCGATGAAGGAGGATGTATCA CGACAGTGAAATCAGCCGT sF 18sR TCAAAGCAAGCCTACGCTCT GCTTTCGCAGTTGTTCGTCT 125

$1 犘犗犇 & E ( < [ H \ +, D E 4 _ U a b ORFW 犘犗犇 ' c,- A [ (X 1)L MPCR,DE11000~1200bpPCR ;(.$

4 s;72 30s, 45 qrt PCR = SYBR Premix Ex Taq TM (TaKaRa)? 10μL,ddH 2O7.4μL, V 0.8μL, 1μL :A c N X 1 A Excel2003 F SPSS17.0 L M () *F O 2 O 2.1 犘犗犇 & E ( < [ H \ +, D E 4 _ U a b ORFW 犘犗犇 ' c,- A [ (X 1)L MPCR,DE11000~1200bpPCR ;(. 1) NCBIBlastn/J :4 < 犘犗犇 ' 01( 犔犪犮狋狌犮犪狊犪狋犻狏犪 ) 犘犗犇 4 ( 犎犲犾犻犪狀狋犺狌狊犪狀狀狌狌狊 ) 犘犗犇 5 2 ( 犌狅狊狊狔狆犻狌犿狉犪犻犿狅狀犱犻 ) 犘犗犇 7 犘犗犇 42 O -, O = 79% 76% 73% F 88% Q ) / J = 犌犼犘犗犇 4 犌犼犘犗犇 5 犌犼犘犗犇 7 F 犌犼犘犗犇 42,GenBank G H I = MH MH MH F MH W CDS = F1005bp, F 334 ' 4 犌犼犘犗犇 ' 345 = ATG, 犌犼犘犗犇 42F 犌犼犘犗犇 = TAA, 犌犼犘犗犇 7F 犌犼犘犗犇 4 M.DL2000;1~4 X 犌犼犘犗犇 5 犌犼犘犗犇 4 犌犼犘犗犇 42F 犌犼犘犗犇 7.1 PCR ;89. M.DL2000;1-4represent 犌犼犘犗犇 5, 犌犼犘犗犇 4, 犌犼犘犗犇 42and 犌犼犘犗犇 7,respectively Fig.1 ElectrophoresisresultofPCRproducts W675 = TGA 2.2 <=>?@ ( 犌犼犘犗犇 2 ( Z & O ^,4 < POD : ;,3 GjPOD5 #, = (X 2) GjPOD4 (Ser) -,Y 12%, (Ala)= 9.9%;GjPOD5 (Leu)F (Ala) -, = 11.2%, (Ser)9.3%;GjPOD7 (Ser) - Y12.8%, (Ala),= 9.3%; Gj POD42 -,Y10.5%, (Ser),=7.2% FGH8I JK LM ^ F ',POD abw (.2), < ' ' F=> ', Ⅲ Z Mo tif ^4 GjPOD 5 W Mo tif(.3) >N O LM I ( V I 4 POD N?W1~26 ' I TMpred O POD #F# W Z@A O ^:GjPOD7 Z (,= 40, GjPOD4 F GjPOD42, =36F35, GjPOD5 Z,= 22 (X3) 2 4% 犌犼犘犗犇 2 ( Table2 PhysicochemicalpropertiesofthefourGjPODs 犌犼犘犗犇 P L ( POD 4 POD Wα FBCD ' GjPOD % W α F41.61%BCD ',E 4.63%W β, 14.81% = ;GjPOD5 W α W -,=41.12%,BCD '=38.32%, β Protein Molecularweight/ku Formula T ( No.ofatoms 8 狆犐 ( Aliphaticindex : ( Instabilityindex GRAVY GjPOD C1498H2392N426O469S GjPOD C1515H2403N431O459S GjPOD C1489H2374N440O466S GjPOD C1656H2641N473O493S

１０ U ;<= M ^_` ４ j 犘犗犇 9k fg ly １７８１ Ð*p±olm ¹ º»p`±² 9ÂÃ F ２ #* 9 Gt ＴｈｕｎｄｎｄｈｎｄｈｈｎｈｄｂｏｘｐｎｈｏｏｘｄｙｐｏｎｖｄｄｏｍｎｎｄｈｍｂｎｄｎｇｏＰＯＤｐｖｙＦ２Ｍｕｏｈｍｎｏｄｇｐｇｎｍｎｑｕｎ F ３ＰＯＤ ¹ º»p ｍｏ ly Ｆ３

@jk Ｔｂ３Ｒｕｏｐｐｄｎｍｍｂｎｕｕｎｄｐｈｏｐｈｏｏｎｎｐｏｄｇｎｙ ª«ＰｏｎＬｎｇｈ Ms¼ Ｓｇｎ ½¾ º» Ｔｎｍｍｂｎｄｏｍｎ Á deâã Ｐｈｏｐｈｏｏｎｙ / 5 ＩｎｄＯｕｄ 5 / ＯｕｄＩｎｄＳＴＹＰＯＤ４３２４１２６２３２８６２ＰＯＤ５３２１１２１４２１４

5 １０ U ;<= M ^_` ４ j 犘犗犇 9k fg ly １７８１ Ð*p±olm ¹ º»p`±² 9ÂÃ F ２ #* 9 Gt ＴｈｕｎｄｎｄｈｎｄｈｈｎｈｄｂｏｘｐｎｈｏｏｘｄｙｐｏｎｖｄｄｏｍｎｎｄｈｍｂｎｄｎｇｏＰＯＤｐｖｙＦ２Ｍｕｏｈｍｎｏｄｇｐｇｎｍｎｑｕｎ F ３ＰＯＤ ¹ º»p ｍｏ ly Ｆ３Ａｎｕｏｈｄｎｄｏｎｖｄｄｏｍｎｎｄｍｏｎｐｏｄｐｏｎｇｙ = ３犘犗犇 b$x yzjvw<{ m)}s~?@jk Ｔｂ３Ｒｕｏｐｐｄｎｍｍｂｎｕｕｎｄｐｈｏｐｈｏｏｎｎｐｏｄｇｎｙ ª«ＰｏｎＬｎｇｈ Ms¼ Ｓｇｎ ½¾ º» Ｔｎｍｍｂｎｄｏｍｎ Á deâã Ｐｈｏｐｈｏｏｎｙ / 5 ＩｎｄＯｕｄ 5 / ＯｕｄＩｎｄＳＴＹＰＯＤ４３２４１２６２３２８６２ＰＯＤ５３２１１２１４２１４７１ＰＯＤ７３２１１２４２１２４１２４ＰＯＤ４２３３４１２１３３１９１２４ pùúª ÿ l m a ４９８ p １５５８ＰＯＤ７ＡＰＲＸ４２ aþ3 Ｇｏｕｐ１ F ４ À f g h è 9 j k p ù ú ª ÿ l m a α β ３８３２４０１９５３０ p １６２ＰＯＤ４２２３ < O P 犘犗犇 T n < 犘犗犇, À f g h è 9 ù ú ª ÿ p β j k l m a α ４０４２３８３２１５２ p ５９９２３１ F 犘犗犇 T n < 犘犗犇, 8 = > ２２５ H?@jk Í/ Äly Å ªæ ＰＯＤ ýe p æ á ß â + F Q ＡＰＲＸ１２ p１９ 5 +gla５ ÆÇÈÉ ５Ａ Øp q Ó*1 9k l y F ５Ｂ犌犼犘犗犇４? ３犘犗犇 9k$%v Ä 3y F ４ ^ _ ` ４ＰＯＤ zp?þq ＰＯＤ４ p ＰＯＤ７ Æ Ç È É ＡＰＲＸ５２ a Þ 3 ＰＯＤ５ Æ Ç È É ＡＰＲＸ４７ aþ3 Ｇｏｕｐ４ＰＯＤ４２ ÆÇÈÉ 8=> LóÛ âãäåæ ＰＯＤ ^_` p æ Z Õ Ó } ª 犘００１ lmra ª ５０ p １０犌犼犘犗犇５? æ Z ÓE ª 犘００５犌犼犘犗犇７? æ

6 １７８２! " # $ % & ３８ è Ａ ÇÈÉ ^_` ±s: ＰＯＤＧｏｕｐ１Ｇｏｕｐ５５ EF ＰＯＤ F ４ＰＯＤ Í/ Ä Ａ犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪犌犲狉犫犲狉犪犼犪犿犲狊狅狀犻犻ＢｏｕＣｈｎｄｐｎｐｎｈｇＰＯＤｊＧｏｕｐ１Ｇｏｕｐ５ｐｎｈｖｍｏＰＯＤｇｏｕｐｊＦ４ＰｈｙｏｇｎｏＰＯＤｇ０５ p ００１ *1 Ó ßà ã p lm ０ F ５ âã täåæ ＰＯＤＡ [ 9k Ｂｎｄｎｄｎｈ００５ｎｄ００１ｖｐｖｍｂｏｗｎｄｇｎｙＴｈＦ５ＴｈＰＯＤｖＡｎｄ犘犗犇ｘｐｏｎｐｎＢｎｄｎｏｇｂｇｇｎｏ Zý E Ù µ õ ª ý } 犌犼犘犗犇４２? q Ó*1 ４ æ p æ Z Õ Ó } ª 犘２３２ 4 ` a 犘犗犇 T n < 犘犗犇, 8 = > ００１ ira ª １０ áì l y ４? â ã ä å æ? ＳＡ ÐÑóP æ ＰＯＤ ç B D G D ÛÜ? ４ｈ ýe rat½ ２５犌犼犘犗犇４２ 9k Æ ＰＯＤ ç Ó è á Ì 犘００５犌犼犘犗犇４ 9 k Æ ＰＯＤ Í F ６Ａ４ j9k l ç C D F G Þ è á Ì Ø p q Ó * 1 犌犼犘犗犇５ p? ４ｈ ZWXDE at½ ３０ Í ８ｈ犌犼犘犗犇７ Æ ＰＯＤ ç u á Ì Ø l p Zat½ ２３ Í F v X G Ø w E t Û Ü F ６Ｂ Ù æ 犌犼犘犗犇４ ý Å

7 107, : <4 犘犗犇 ' WBC XY O 1783 犌犼犘犗犇 5XY SA 4h. J 犌犼犘犗犇 7 犌犼犘犗犇 42WXY SA 8hYG -,H=J \W2,T.D J O (X4)X : SA V,4 犌犼犘犗犇 ' XY POD N, 犌犼犘犗犇 7 犌犼犘犗犇 42 W X Y N ( 犘 < 0.01) -A PEG P, ^: PEG _ V,POD * +,. / W Z, - J, 3h Y G O Y,H = J W2 (.6,A) 犌犼犘犗犇 4' WXY 1h Z, 2 F6h (H=J W3 ); 犌犼犘犗犇 5' WXY PEG 7(1h)F6 h J, 2 F 3h - J ; 犌犼犘犗犇 7 犌犼犘犗犇 42 W X Y 3h - J (. 6,D) O (X 4) ^: PEG _ V, 犘犗犇 ' WXY B, 犌犼犘犗犇 7 犌犼犘犗犇 42 W X Y N ( 犘 < 0.01), 犌犼犘犗犇 4 犌犼犘犗犇 7 犌犼犘犗犇 4 犌犼犘犗犇 42WXY N K 4 犘犗犇 6 犘犗犇 + ( Table4 CorrelationanalysisbetweenPODactivityand 犘犗犇 expressionlevel! Category % Index POD PODactivity 犌犼犘犗犇 42 ' XY Geneexperessionlevel 犌犼犘犗犇 4 犌犼犘犗犇 5 犌犼犘犗犇 7 POD PODactivity 1 Organ 犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇 POD PODactivity 1 SA SAtreatment 犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇 POD PODactivity 1 PEG PEGtreatment 犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇 POD PODactivity 1 Cold treatment 犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇 POD PODactivity 1 犘犺狔狋狅狆犺狋犺狅狉犪犮狉狔狆狋狅犵犲犪 treatment 犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇犌犼犘犗犇 : X^ 0.05 ( ), X^ 0.01 Note: indicatessignificantcorrelationatthe0.05level(bilateral), indicatesignificantcorrelationatthe0.01level

8 V, < POD, /, W Z, - J, 12h YG -,= J \ W 3 (. 6,A) 犌犼犘犗犇 4 ' W X Y, /, W Z, 8h J ; P Q$ 犌犼犘犗犇 5 犌犼犘犗犇 7F 犌犼犘犗犇 42' WXY, 12 h WXY J J V/12 11 F 11, 犌犼犘犗犇 5 12h X Y (. 6,C) O (X4)X : 犘犗犇 ' WXY POD N, 犌犼犘犗犇 5 犌犼犘犗犇 7 犌犼犘犗犇 5 犌犼犘犗犇 42WXY N ( 犘 < 0.05), 犌犼犘犗犇 7 犌犼犘犗犇 42 W X Y N ( 犘 < 0.01) Z [ \ 2 ] " # $ 犘犗犇 6 犘犗犇 & '^_ c 2d., < * R W, Q POD 9:,-,H=J W2,T. 4V/ M- J (. 6,A) 犌犼犘犗犇 4 犌犼犘犗犇 7 F 犌犼犘犗犇 42WXY QRSW 2 +,./ W Z, 4d XY -, H = J W3 3 F4,=2d W F1.2 ; 犌犼犘犗犇 5 D *, /, W Z,2d WXY -,H=J \W2, ( 犘 < 0.01),4d W X Y J \,6d WXY L- J \(.6,E) O (X4)X : _ V, 4 ' WXY LFYG, W, 犌犼犘犗犇 7 犌犼犘犗犇 42 W X Y N ( 犘 < 0.05), 犌犼犘犗犇 4 犌犼犘犗犇 5WXY N WK 3 S T POD a Z W U T $+' <[H\,]^DE4 U a b CDSW 犘犗犇 ' ( 犌犼犘犗犇 4 犌犼犘犗犇 5 犌犼犘犗犇 7 F 犌犼犘犗犇 RT PCR / A. ;B. ;C. (4 ) P; D.PEG ;E.STU.6 V POD (A) 犌犼犘犗犇 ' (B~E)XY A.Thediferenttreatments;B.SAtreatment;C.Cold(4 ) stress;d.pegtreatments;e.pathogentreatment Fig.6 ThePODactivities(A)and 犌犼犘犗犇 gene expressionpaterns(b-e)underdiferenttreatments < BC D E K ' cdna c c O X K4 犘犗犇 ' : W Ⅲ Z JK ' LM, NFXY O, ' POD, GjPOD VW <a, # $+$ K4 犌犼犘犗犇 ' W_`X :

9 107, : <4 犘犗犇 ' WBC XY O 犌犼犘犗犇 &`ab "#$c ClassⅢ POD =:[,` c 9 F', _`,` VW c & 犘犗犇 ' E 8 Y, 8 c, P Y, a 2. A [5,24 25] X Y 犃犘犡 ' W 4 - V ( )cw [' ()W4Z - [I 7 W 4 Z F [26 28] $ + W $ + (PEG )_ V 犌犼犘犗犇 ' <* XY, ^ <, P A POD a S 2 W. A \ c E G [21 22,25,29 30] Hilaier [29] K ] S U. 犗狊犘犚犡 114 X Y; Choi [25] ^ _ S T U W 2, 犆犪犘 02' ` H 2O 2, H 2O 2 ST ' WXY5S $+, 犌犼犘犗犇 4 犌犼犘犗犇 7 F 犌犼犘犗犇 42 WXY N G Q R S U J 5, 犌犼犘犗犇 5 KS`7(2d),0 < Q R S U W 2. A 3.2 犌犼犘犗犇 5 犌犼犘犗犇 7 犌犼犘犗犇 42 8' O < 4 _ 犘犗犇 ' c, a W F motif, ^ W_`F A `/ O ' XY O,K ' 犌犼犘犗犇 5 犌犼犘犗犇 7F 犌犼犘犗犇 42 VW Z C #, O X WXY c V,0 TN ` _`X 3.3 犌犼犘犗犇 4 RS' W POD,?@& Z b F W?@,?@ ` _` $+?@ ^ GjPOD4-. F@ #, ^ ` # W PRX,?@ W, %& W ' [30] 犌犼犘犗犇 4' W X Y 3 V 犌犼犘犗犇 7F 犌犼犘犗犇 42 #, _ V 3 犌犼犘犗犇 SA 7 W 犌犼犘犗犇 4 G J 犌犼犘犗犇, ` DE a [17] X 犌犼犘犗犇 4W_` ` 犌犼犘犗犇 : b 1: [1] WELINDER K G.Superfamilyofplant,fungalandbacterial peroxidases[j]. 犆狌狉狉. 犗狆犻. 犛狋狉狌犮狋. 犅犻狅犾.,1992,2(3): [2] PASSARDIF,PENELC,DUNAND C.Performingthepara doxical:how plant peroxidases modify the cel wal[j]. 犜狉犲狀犱狊犻狀犘犾犪狀狋犛犮犻犲狀犮犲,2004,9(11): [3] TOGNOLLIM,PENELC,GREPPIN H, 犲狋犪犾.Analysisand expressionoftheclassⅢ peroxidaselargegenefamilyin 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 [J]. 犌犲狀犲,2002,288(1 2): [4] COSIO C,DUNAND C.Transcriptomeanalysisofvarious flowerandsiliquedevelopmentstagesindicatesasetofclass Ⅲ peroxidasegenespotentialyinvolvedinpodshateringin 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 [J]. 犅犕犆犌犲狀狅犿犻犮狊,2010,11(1):528. [5] WANG Y,WANG Q,ZHAO Y, 犲狋犪犾.2015.Systematicanal ysisofmaizeclass Ⅲ peroxidasegenefamilyrevealsacon servedsubfamilyinvolvedinabioticstressresponse[j]. 犌犲狀犲, 566(1): [6],,,.Class Ⅲ Z W A $+L [J].,2011,31(9): MENG YY,FANSL,SONG MZ, 犲狋犪犾.Advanceinresearch onclass Ⅲ peroxidasesanditsfunctioninplants[j]. 犃犮狋犪犅狅狋犪狀犻犮犪犅狅狉犲犪犾犻犗犮犮犻犱犲狀狋犪犾犻犪犛犻狀犻犮犪,2011,31(9): [7],,,. Z ⅢW, N O[J]. bc,2015,13(11): WEIL,ZHANGL,WANG W W, 犲狋犪犾.Bioinformaticsanaly sisofclass Ⅲ peroxidasesin 犌犾狔犮犻狀犲犿犪狓 [J]. 犕狅犾犲犮狌犾犪狉犘犾犪狀狋犅狉犲犲犱犻狀犵,2015,13(11): [8] KASRAOUI M F,DUQUESNOYI,WINTERTON P, 犲狋犪犾. Solubleandcelwalboundperoxidaseactivitiesaremarkersof flowerbuddevelopmentstagesinlemon ( 犆犻狋狉狌狊犾犻犿狅狀 L.) [J]. 犑狅狌狉狀犪犾狅犳犃狆狆犾犻犲犱犅狅狋犪狀狔犪狀犱犉狅狅犱犙狌犪犾犻狋狔,2014,87: 1 8. [9] HERROJ,ESTEBAN CARRASCO A,ZAPATAJ M.Loo kingfor 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 peroxidasesinvolvedinlignin biosynthesis[j]. 犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔犪狀犱犅犻狅犮犺犲犿犻狊狋狉狔,2013, 67(3): [10] FERNANF,VIVART,POMARF, 犲狋犪犾.Peroxidase4isin

10 volvedinsyringylligninformationin 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 [J]. 犑狅狌狉狀犪犾狅犳犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,2015,175: [11] YOSHIDA K,KAOTHIEN P,MATSUIT, 犲狋犪犾.Molecular biologyandapplicationofplantperoxidasegenes[j]. 犃狆狆犾犻犲犱犕犻犮狉狅犫犻狅犾狅犵狔犪狀犱犅犻狅狋犲犮犺狀狅犾狅犵狔,2003,60(6): [12] BADERB,AISSAOUIF,KMICHAI, 犲狋犪犾.Efectsofsalini tystressonwaterdesalination,olivetree ( 犗犾犲犪犲狌狉狅狆犪犲犪 L. cvs Picholine, Meski and Ascolana )growthandionac cumulation[j]. 犇犲狊犪犾犻狀犪狋犻狅,2015,364: [13] AHMED CB,ROUINA BB,SENSOYS, 犲狋犪犾.Changesin gasexchange,prolineaccumulationandantioxidativeenzyme activitiesinthreeolivecultivarsundercontrasting watera vailabilityregimes[j]. 犈狀狏犻狉狅狀犿犲狀狋犪犾犪狀犱犈狓狆犲狉犻犿犲狀狋犪犾犅狅狋犪狀狔,2009,67(2): [14] MOKHTAR G,OLFA B,DALENDA B, 犲狋犪犾.Impactsof waterstresson gasexchange,waterrelations,chlorophyl contentandleafstructureinthetwomaintunisianolive( 犗犾犲犪犲狌狉狅狆犪犲犪 L.)cultivars[J]. 犛犮犻犲狀狋犻犪犎狅狉狋犻犮狌犾狋狌狉犪犲,2009, 119(3): [15] RODR?GUEZ CALCERRADAJ,BUATOISB,CHICHEE, 犲狋犪犾.Leafisopreneemissiondeclinesinquercuspubescens seedlingsexperiencingdrought anyimplicationofsolublesug arsandmitochondrialrespiration[j]. 犈狀狏犻狉狅狀犿犲狀狋犪犾牔犈狓狆犲狉犻犿犲狀狋犪犾犅狅狋犪狀狔,2013,85(1): [16],, M,. V Z ' WXY Ja Q A W [J].7 8,2016,43(8): WEIS,SHIQ Q,MA Y Q, 犲狋犪犾.Efectsofperoxidasegene totheresistanceof 犆狌犮狌犿犻狊犿犲狋狌犾犻犳犲狉狌狊 against 犕犲犾狅犻犱狅犵狔狀犲犻狀犮狅犵狀犻狋犪 indiferenttemperature[j]. 犃犮狋犪犎狅狉狋犻犮狌犾狋狌狉犪犲犛犻狀犻犮犪,2016,43(8): [17],,,.() J F PW / [J].' \ A,,2015, 34(3): YANG H Q,WANGSS,ZHANGJB, 犲狋犪犾.Diferentialre sponseandcomparisonofpolyphenolsmetabolismintobacco seedlingunderthestressesofdroughtandlowtemperature [J]. 犌犲狀狅犿犻犮狊犪狀犱犃狆狆犾犻犲犱犅犻狅犾狅犵狔,2015,34(3): [18],,,.5 PJ 2 Z W. A,,2003,14(6): LIG Q,ANSQ,ZHANGJL, 犲狋犪犾.Impactofsaltstresson peroxidaseactivityin 犘狅狆狌犾狌狊犱犲犾狋狅犻犱犲狊 cambiumanditscon sequence[j]. 犆犺犻狀犲狊犲犑狅狌狉狀犪犾狅犳犃狆狆犾犻犲犱犈犮狅犾狅犵狔,2003,14 (6): [19] BLOKHINA O,VIROLANINEN E,FAGERSTEDT K V. Antioxidants,oxidative damage and oxygen deprivation stress:a review [J]. 犃狀狀犪犾狊狅犳犅狅狋犪狀狔,2003,91 (2): [20] CHENG C,ZHANG Y,ZHONG Y, 犲狋犪犾.Geneexpression changesinleavesof 犆犻狋狉狌狊狊犻狀犲狀狊犻狊 (L.)osbeckinfectedby 犆犻狋狉狌狊狋狉犻狊狋犲狕犪狏犻狉狌狊 [J]. 犑狅狌狉狀犪犾狅犳犘狅犿狅犾狅犵狔牔犎狅狉狋犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲,2016,91(5): [21] 3,,,. Z ' 犛犮犘犗犇 02 WBC _` [J].,2017,43(4): SU Y C,WANG Y Q,LIZ, 犲狋犪犾.Molecularcloningand functionalidentificationofperoxidasegene 犛犮犘犗犇 02inSug arcane[j]. 犃犮狋犪犃犵狉狅狀狅犿犻犮犪犛犻狀犻犮犪,2017,43(4): [22] ZHANGSP,XIAO Y N,ZHAOJR, 犲狋犪犾.Digitalgeneex pressionanalysisofearlyrootinfectionresistanceto 狊狆狅狉犻狊狅狉犻狌犿狉犲犻犾犻犪狀狌犿 f.sp. 狕犲犪犲 in maize[j]. 犕狅犾犲犮狌犾犪狉犌犲狀犲狋犻犮狊牔犌犲狀狅犿犻犮狊,2013,288(1 2): [23],, 3. <c& $+L [J].,2014,5: LIY Q,L CP,HUC M.Advancesingermplasmresources of 犌犲狉犫犲狉犪犼犪犿犲狊狅狀犻 [J]. 犎狌狀犪狀犑狅狌狉狀犪犾狅犳犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲狊,2014,5: [24] LIU B,XUEX,CUIS, 犲狋犪犾.Cloningandcharacterizationof a wheatβ 1,3 犵犾狌犮犪狀犪狊犲 geneinduced bythestriperust pathogen 犘狌犮犮犻狀犻犪狊狋狉犻犳狅狉犿犻狊 f.sp. 狋狉犻狋犻犮犻 [J]. 犕狅犾犲犮狌犾犪狉犅犻狅犾狅犵狔犚犲狆狅狉狋狊,2010,37(2): [25] CHOIH W,HWANGBK.Hydrogenperoxidegenerationby thepepperextracelularperoxidasecapo2activateslocaland systemicceldeathanddefenseresponsetobacterialpatho gens[j]. 犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,2007,145(3): [26] HIROOKA S,MISUMIO,YOSHIDA M, 犲狋犪犾.Expression ofthecyanidioschyzon merolaestromalascorbateperoxidase in 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 enhancesthermotolerance[j]. 犘犾犪狀狋犆犲犾犚犲狆狅狉狋狊,2009,28(12): [27] SUN W H,DUAN M,LIF, 犲狋犪犾.Overexpressionoftomato 狋犃犘犡 geneintobaccoimprovestolerancetohighorlowtem peraturestress[j]. 犅犻狅犾狅犵犻犪犘犾犪狀狋犪狉狌犿,2010,54(54): [28] SATO Y,MASUTA Y,SAITO K, 犲狋犪犾.Enhancedchiling toleranceatthebootingstageinricebytransgenicoverex pressionoftheascorbateperoxidasegene, 犗狊犃犘犡犪 [J]. 犘犾犪狀狋犆犲犾犚犲狆狅狉狋狊,2011,30(3): [29] HILAIREE,YOUNGSA,WILLARDL H, 犲狋犪犾.Vascular defenseresponsesinrice:peroxidaseaccumulationinxylem parenchymacelsandxylem walthickening[j]. 犕狅犾. 犘犾犪狀狋犕犻犮狉狅犫犲犐狀狋犲狉犪犮狋,2011,14(12): [30] RENLL,LIU YJ,LIU HJ, 犲狋犪犾.Subcelularrelocalization andpositiveselectionplaykeyrolesintheretentionofdupli categenesofpopulusclass Ⅲ peroxidasefamily[j]. 犘犾犪狀狋犆犲犾,2014,26(6): (:)

BuletinofSoiland WaterConservation Vol.38,No.3 Jun.,2018 & X R( + 1, 1, < 1,=> 3 (1. E^ IFTU ^,NO ; 2.NO\ ^,NO ;3.NO B`a -

$BuletinofSoiland WaterConservation Vol.38,No.3 Jun.,2018 & X R( + 1, 1, < 1,=> 3 (1. E^ IFTU ^,NO ; 2.NO\ ^,NO ;3.NO B`a -$ 383 0186 BuletinofSoiland WaterConservation Vol.38,No.3 Jun.,018 & X R( + 1, 1, < 1,=>,?@A 3 (1. E^ IFTU ^,NO 730070;.NO\ ^,NO 730070;3.NO B`a -[/H,NO 730000) -.:[# ] _D5 & % I /, + -. I P+- [ ] ( 犜狉犻狋犻犮狌犿犪犲狊狋犻狏狌犿