EHPL

Size: px

Start display at page:

Download "EHPL"

耗骐何
9 years ago
Views:

1 ...4 EHPL,2: (EHPL, 2:1)

2 EHPL

3 :. 45 2:

4 ,Hypericum perforatum L HPL St. John s wort, SJW hypericin, hyperforin pseudohypericin, EHPL [1] [2][3] (Lysine hydrochloride) 1:1,2:1,1:2 1 Herpes simplex virus, HSV-1 (Varicella-Zoster virus, VZV) : 2:1( ) HSV-1 VZV VZV Post-herpetic trigeminal neuralgia,phn PHN Human immunodeficiency virus HIV [4] HIV [5] HIV PHN 4

5 5

6 EHPL,2: Extract of Hypericum perforatum L, EHPL, Lysine hydrochloride, Indomethacin, Aspirin,, , carboxymethylcellulose sodium CMC-Na, :1, 0.25% CMC 0.25% CMC 0.25% CMC 1.2. ICR g 20 2, 1 12 SCXK AB104-N Mettler-Toledo HH.BII420 50µl 6

7 [6] mg/kg 250mg/kg 500mg/kg CMC 5mg/kg (ip) (ig) % ( )20µl [7] mg/kg 250mg/kg 500mg/kg CMC 10mg/kg (ip) (ig) l 6mm 2.2 [8][9][10] 5 125mg/kg 250mg/kg 500mg/kg CMC ( ) 300mg/kg (ig) 50mg/kg mg 6mm, 12 h 12h 7

8 60 24h mg( )/10g( ) s Excel-2000 t, p< Table 1 Analgesic effects of Laijinsi on the formaldehyde-induced nociceoptive response in mice ( s, n=10) group dose 0~5min 15~30min mg/kg/d Time(sec) Inhibition(%) Time(sec) Inhibition(%) 7d ig Vehicle (0.25%CMC) Morphine 5 (ip) 1d *** *** Laijinsi * * ** *p<0.05, **p<0.01 and ***p<0.001 vs vehicle; Student s t-test. 8

9 the time of licking the hind paw(sec) cmc *** *** morphine5 Laijinsi125 * Laijinsi250 * Laijinsi500 ** 0~5min 15~30min group(mg/kg) *p<0.05,**p<0.01 and ***p<0.001,vs vehicle ;Student's t-test. Fig. 1 Analgesic effects of Laijinsi in formaldehyde-induced nocicepetive response in mice( s,n=10)., 125mg/kg, 250mg/kg, 500mg/kg 15~30 0~5 9

10 Table 2 Anti-inflammatory effects of Laijinsi on the formaldehyde-induced paw edema in mice ( s,n=10) group Dose(ig) Weight edema Inhibition(%) Vehicle (0.25%CMC) (mg/kg/d 7d) (mg) morphine 5 (i.p) 1d * Laijinsi * * ** *p<0.05 and **p<0.01, vs vehicle; Student s t-test weight edema(mg) * * * ** 10 0 cmc morphine group(mg/kg) *p<0.05 and **,p<0.01,vs vehicle ;Student's t-test. Figure 2. Anti-inflammatory effects of Laijinsi on the formaldehyde-induced paw edema in mice ( s,n=10) 10

11 mg/kg, 250mg/kg, 500mg/kg 3.2 Table 3 Anti-inflammatory effects of Laijinsi on xylene-induced mice ear edema ( s, n=10) group Dose Weight edema inhibition mg/kg/d 7d ig mg Vehicle (0.25%CMC) Indomethacin 10 (i.p) 1d *** Laijinsi ** *** ** **p<0.01and ***p<0.001, vs vehicle, Student s t-test weight edema mg) 9 6 *** ** *** ** 3 0 cmc indo Laijinsi group(mg/kg) **p<0.01 and ***p<0.001, vs vehicle ;Student s t-test. 11

12 Fig. 3. Anti-inflammatory effects of Laijinsi on xylene-induced mice ear edema ( s, n=10). 3 3 CMC 125mg/kg 250mg/kg 500mg/kg 250mg/kg 50.69% ip (75.35%) 3.3 mice.( Table 4 Anti-inflammatory effects of Laijinsi on slip-induced granuloma in group s,n=10) Vehicle (0.25%CMC) dose (mg/kg/d 7d,ig) index of granuloma proliferation(mg/10g) Aspirin * Laijinsi * p<0.05 vs vehicle, Student s t-test * * * inhibition 25 index of granuloma proliferation(mg/10g) * * * * 0 CMC aspirin300 Laijinsi125 Laijinsi250 Laijinsi500 group(mg/kg) *p<0.05 vs vehicle, Student s t-test 12

13 Fig. 4. Anti-inflammatory effects of Laijinsi on slip-induced granuloma in mice.( ±s,n=10) 4 4 CMC 500mg/kg 250mg/kg 125mg/kg LD g/kg [11] LD 50 (11.73g/kg) 4. (EHPL,2:1) Extract of Hypericum perforatum L, EHPL, Lysine hydrochloride,, Aspirin,, , carboxymethylcellulose sodium CMC-Na,,970826,Evan s, , Sigma 2:1 0.25% CMC 0.25% CMC 0.25% CMC 13

14 1.2. ICR g SCXK mL AB104-N Mettler-Toledo TU Lingli LDZ [12][13] mg/kg 250mg/kg 500mg/kg CMC 300mg/kg (ig) 7 1h 1% Evan s 0.1mL/10g 0.6% 0.1mL/10g, 20min 6mL ml 0.1N NaOH 10mL 2 h nm A A Evan s [14][15] mg/kg EHPL166mg/kg 333mg/kg 666mg/kg CMC 300mg/kg( ) ig % ( )50µl 4 2.5mL 1h 3000rpm/min 15min 0.5mL 0.5mol/L KOH- 2mL 50 20min 5mL UV nm A A PGE 2 14

15 s Excel-2000 t, p< Table 5 Inhibitory effect of Laijinsi on acetic acid-induced increase of vascular permeability in mice.( s,n=10) group Dose capillarity inhibition % (mg/kg/d 7d, ig) permeability (A) Vehicle (0.25%CMC) aspirin ** Laijinsi * * *p<0.05 and **p<0.01,vs vehicle Student s t-test 15

16 0.2 capillarity permeability A ** * * 0 cmc aspirin300 laijinsi125 group(mg/kg) laijinsi250 Laaijinsi500 *p<0.05 and **p<0.01 vs vehicle Student s t-test. Fig. 5. Inhibitory effect of Laijinsi on acetic acid-induced vascular permeability in mice.( s,n=10) mg/kg 250mg/kg therapeutic index TI (TI=LD 50( )/ED 50( )) 16

平安（备案）[2009]N237号-平安全球医疗保险条款

平安（备案）[2009]N237号-平安全球医疗保险条款中国平安财产保险股份有限公司平安全球医疗保险条款第一条总则在投保人向保险人提出投保申请并如约支付保险费的前提下, 保险人同意出具本保险合同, 并承诺根据本保险合同的约定为被保险人提供保障第二条年龄限制本保险被