11 ', : VW 781 b cbco 9ST :); 1.1 V W +! " -. / 0,_ W 70%! " C -. / 0 =, BJ cfu/g :; D E < $ , F 5G,H:

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1 38 11 Vol.38No ActaEcologiaeAnimalisDomastici Nov.2017!"#$ %&' () *+, 1, 1, 1, 1, 1, 1,! 2,"#$ 1,%&' 1 (1.!"#$%&', ; 2. ()*+,-./0, ) [-.] ()*+,-./ :; <=9> <?@A,BC25 D56? E : 78150, FG 4HI J9KI II IIL II,MI3NO,MNO 10,4HI,J 9KI *+ 100mg/kgPQ3K,RS3IGT *+1000mg/ kg,-./01 23,-./ UVW30d XY :(1) 4HI,J9KI ⅠILⅢI ZO(ADG)[\]Z+( 犘 <0.01),ⅡI ADG \]Z+( 犘 <0.05);^ U V I 7 8 _ ` a [ \ ] b c( 犘 <0.01);(2)I IIIgA IgM \]Z+( 犘 <0.05),IIIgA IgM LIgG [\]Z+( 犘 <0.01);(3)I II C3 C4 [\]Z+( 犘 <0.01),II C3\]Z+( 犘 <0.05);(4)I IIIL 2 IL 4 IFN γ\]z+ ( 犘 <0.05),IIIL 2 IL 4 IFN γ[\]z+( 犘 <0.01);(5)^UVI (ALB) [\] Z+( 犘 <0.01);II K (BUN)\]bc( 犘 <0.05),J9KI IL II K (BUN)[ \]bc( 犘 <0.01), 5678 ()*+,-./ :; =, 9> <, Y *+ [/01],-./01 ; 23;5678;9:; ; <;9> < [ ] S811.5 [ 2345] A [ 67 ] (2017) :;, <=> 7,?@ABC DE +,1 + BC>,FG1 HIJKL MNO PQ,R$STU 1 + [1 2] VW X Y Z[\],^)_`a1 b c <B C,- BC> V, ]VW [2] VW [3] VW [4] VW^)_ 1 b c <BC,- 1 BC> ; -, \]X Y )_`]1 BC,,`a <, 1 [5 7] VW X Y > 781 BCO N 9ST F! ( 狆狊犲狌犱狅狊狋犲犾犪狉犻犪犺犲狋犲狉狅狆犺狔犾犪 (Miq.)paxexpaxetHofm)9, V W - > ` a B C O [8] 9 J 60%_, 4900t [9], V,, 9VW)_- 1 b cbco, N [10] [11], )_ 1,`a1 BC _781 ', \] VW, 781 b BC O c N 9ST, VW > 781 = [ ] : [ ] "# $!(2014N5004) [ %&] ' (1991-),, (,, )* +, E mail: @163.com [-. ] /01(1970-),, (,, 2 F3, )*: +, E mail:[email protected] 456(1954-),,7 (,,82 F3, )*; +, E mail:[email protected]

2 11 ', : VW 781 b cbco 9ST :); 1.1 V W +! " -. / 0,_ W <,=>-?@;A9VWBJ 70%! " C -. / 0 =, BJ cfu/g :; D E < $ , F 5G,H:JK ;'Ⅰ G:JK +1000mg/kg VW; :JK +100mg/kgMNL; 'ⅡG:JK mg/kg ;'ⅢG:JK +1000mg/kg VW 1000 mg/kg,og3 $,O $ 10 ' H I :,@ PQ,RSI TUVWX2Y BC C,' J5d, 30d G cz[q\ <1 =>?@AB CDE ( ; =>) ',]O $ D <$ 93 1, ^_`H 4~5mL, Table1 Compositionandnutrientlevelsofthebasaldiet(DM basis) GFIngredients 30~45d 45~60d Z[Q\ Nutrientlevels 2 30~45d 45~60d 0a Com MN DE/(MJ/kg) bc Soybeanmeal CP/% N b Extrudedful fatsoybean Lys/% Driedwhey Met/% Plasmaproteinpowder 5.00 Thr/% Fishmeal Try/% 0.24 Citricacid Ca/% W Sugar P/% : premix 1 ) Total :1 : O 10mg,!L 0.096mg,Cu30mg,Fe120mg,Mn40mg,Zn80mg,Se0.3mg 2MN, &A Notes:1Thepremixprovidedthefolowingperkgofdiets:VA15000IU,VD8000IU,VE60IU,VK2.5IU,VB12mg, VB24mg,VB62mg,VB1230ug,nicotinicacid10.4mg,D pantothenicacid10mg,biotin0.096mg,cu(ascoppersulfate)30 mg,fe(asferroussulfate)120mg,mn (asmanganesesulfate)40mg,zn(aszincsulfate)80mg,se(assodiumselenite)0.3 mg 2DEwascalculated,othersweremeasured. 3000r/min 10 min,,eppendorfw F,-20 ", A :; < '1 ',] F $, _ \^ `$ (ADG),' G9 HIJ _ G9\^ H I J(ADFI) : $ (F/G) ' O 1 9 Y PQ, 1, _ (%)= [(O )/ (' ' )] BC (IgG IgM IgA) < C3 C4 BJ^H BC ;A, / 0,A RT NF, < S ELISA ; A IL 2 IL 4 IFN γb J, ^! (STAT FAX2100,AwarenessTechnologyInc.USA), < S > < N (TG) (TP) (ALB) (CREA) L (BUN) (ALP) b (AST) (ALT) ' A, ( N -./0,A N BCF 1.4 ' \^ ±,H SPSS 17.0 ) F (one way ANO VA),Duncan; V$, 犘 <0.05, 犘 <0.01

3 34 #$ Y 38 2, F * F 2,, ADFI 21.99%( 犘 <0.05), ⅡG ⅢG ( 犘 > 0.05); 'G ADG F 24.98%( 犘 <0.01) 18.54%( 犘 <0.01) 14.05%( 犘 <0.05) 20.19%( 犘 < <2 A$%&' *+, 0.01); ), ' G F K L41.11% 64.72% 58.62% 76.39%, 'G ^ ( 犘 >0.05); 'G F/G N GH (IJKLMNO F 3), I G, ' GIgA B JF U 8.7% ( 犘 >0.05) 60.40% ( 犘 < 0.01) 32.86%( 犘 <0.05) 72.53%( 犘 <0.01); Table2 Efectsofdiferenttreatmentgroupsongrowthperformanceofweanedpiglets $Initialbodyweight/kg 8.07± ± ± ± ±0.93 $ Finalbodyweight/kg Aa ± Bb ± ABb ± ABb ± ABb ±2.46 \^ HIJ ADFI/g Aa ± Ab ± Aab ± Aab ± Aab ±94.53 \^ `$ ADG/g Aa ± Bb ± BCb ± ACb ± BCb ±63.54 :$ F/G 1.71± ± ± ± ±0.11 Diarhearate/% 3.77 Aa ± Bb ± Bb ± Bb ± Bb ±0.31 : ( 犘 <0.05), ( 犘 <0.01) K Note:Inthesamerow,valueswithdiferentsmalletersuperscriptsmeansignificantdiference( 犘 <0.05),anddiferent capitalletersuperscriptsmeanextremelysignificantdiference( 犘 <0.01).Thesamebelow. <3 A$%&' GH ( 3*+, Table3 Efectsofdiferenttreatmentgroupsonserumimmuneindexesofweanedpiglets mg/l IgA Aa ± Aab ± Bcd ± ABbc ± Bd ±24.77 IgM Aa ± ABab ± ABbc ± ABbc ± Bc ± IgG Aa ± Aa ± ABab ± ABa ± Bb ± C Aa ± Aab ± BCc ± ABbc ± Cd ±24.34 C Aa ± Aa ± Bb ± ABa ± Cc ±12.82 IgG ), I G,IG 18.13% ( 犘 <0.01), L G I IG N; IgM ), 'GF U5.8%( 犘 >0.05) 19.36%( 犘 <0.05) 20.39% ( 犘 <0.05) 23.77% ( 犘 <0.01); C3 ), ' G F 9.3%( 犘 >0.05) 38.90%( 犘 <0.01) 20.61%( 犘 <0.05) 59.70%( 犘 <0.01); ' G < C4 F 4.7% ( 犘 >0.05) 53.96% ( 犘 <0.01) 23.20%( 犘 >0.05) 88.26%( 犘 <0.01) GH PQO F 4),, 'GIL 2 F 30.16%( 犘 >0.05) 57.18%( 犘 <0.01) 23.33%( 犘 >0.05) 66.39%( 犘 <0.01); IL 4 ), ' G F 22.25% ( 犘 >0.05) 30.62%( 犘 <0.05) 32.54% ( 犘 <0.05) 46.89% ( 犘 <0.01); IFN γ), I IGF 1.4% ( 犘 >0.05) 9.28% ( 犘 <0.05) 30.37%( 犘 <0.01) GHF 3O F XG 781 N 9ST 5 5 ) _, I G, ' G TG B J F K L 50.75% 34.33% 34.33% 37.31%( 犘 <0.01); BUN ), 'GF K L 26.49% ( 犘 <0.01) 19.54% ( 犘 <0.05) 26.49%( 犘 <0.01) 26.82% ( 犘 <0.01); ALB), ' G F 14.21% 13.10% 15.24% 14.94%( 犘 <0.01);TP CREA B J c ALP AST ALT bn ( 犘 >0.05) 3 3.1!"#$%&' *+, VW5) - MN! 9 F ", [ F 9 M N (, 1

4 11 ', : VW 781 b cbco 9ST 35 <4 A$%&' GH PQ*+, Table4 Efectsofdiferenttreatmentgroupsonserumcytokinesinweanedpiglets ng/ml IL Bb ± BCbc ± ACac ± Bb ± Aa ±3.25 IL BCc ± ACbc ± ACab ± ABab ± Aa ±1.06 IFN γ BCb ± ACbc ± ABac ± ACab ± Aa ±7.23 <5 A$%&' GHF 3*+, Table5 Efectsofdiferenttreatmentgroupsonserumbiochemicalindexesofweanedpiglets TG/(mmol/L) 0.67 Aa ± Cc ± Bb ± Bb ± Bb ±0.09 ALP(U/L) ± ± ± ± ±21.29 L BUN/(mmol/L) 3.02 Aa ± Bb ± ABb ± Bb ± Bb ±0.34 TP/(g/L) 50.86± ± ± ± ±5.22 ALB/(g/L) Aa ± Bb ± Bb ± Bb ± Bb ±1.65 AST/(U/L) 53.11± ± ± ± ±9.58 ALT/(U/L) 56.88± ± ± ± ±6.84 CREA/(mmol/L) Aa ± Bb ± ABab ± ABab ± ABab ±7.89 ADG [12], 5) % & ',`] %& ' ', [13] CVW 4 V W V W ^ 1 ADG,L [14 15] ( - )MN *9-(X!,+,--X!. / 01, MN \, < 0 cj:92, 34 5M N $ [6] V,8 [16] [5] [17]^ 1 b,l ',,, / :/ \] VW,^ 1 ADG,L, 1 b,;5, ( VW < M N D E ` a <, `]MN -(, --, + \ ] 0.1% V W 0.1% 9'G G ), VW 9 > 5-3.2!"#$%&' (IJKLMNO *+, BC <0= + > b,?,: <9@2 MN AB34" C,/ < < D B C 9 E [18] < C3 < C4 < B T ` [19], N U 9 < < < 9 B C >, <_c F M, 1,`a < [20] V W ) _ F F b ` 9+H b`a, BC `,`]BC BJ, <BC [21 22] X Y I- A JEBC,,K F b, +LM> BCN;?,:)`a T B 1 9 O > b, < B C > [23]?( ) N ;! * ph, 9, < VW92 (, XJ L ) (! *9`, G `a! <9BCO,!9 + [24] ', VW < 1 BC < C3 C4 BJ( 犘 <0.01), \],, ) / VW, `a <BC>, BC b 1 BC 9`], U1 BC 3.3!"#$%&' PQO *+, IL 2/ N 9 CD4+T CD8+T 95, IL 4/ NU9 T P_c P F" IL 2 IL 4 E 4 O / <9BC> O> + 2,)QF T RS TL NK 9 +H b, <BC 9F",R?U P RSIFN γ [25] V W [2] +

5 36 #$ Y 38 \] ] V W ) _ 1 IL 2 IFN γ BJ,`a781 BC Sun [26] +, \]XJ9Yb < 78 IFN γbj,`] IL 4 BJ ',I IG IL 2 IL 4) ^-2',, IG < IL 2 IL 4 IFN γ B J, ^ G,;5, BC, 5G, VW ), U1 BCO 3.4!"#$%&' GHF 3*+, TG BJ 5 2' )_O 1 <* Z Q,[ TG \ ] Z9 ^_`ak L [27] ',I I IG TG B J ^ ( 犘 <0.01),'G, VW ^ 1 Z, = < 39 J, 1 9 TP ALB b'c O U < 92 cf Q [28] 9 BUN 9Q\O 9, BUN 9L O U ` a,o, [28] ', ' G ^ ) _ 1 TP ALBBJ,L BUN BJ, U VW ^)_ < 9, 1 9 D 9 ALP E 4,[ b, B - b,, ALP b,a ALP b)o b 2' [29] ALT AST / < bn K/ O H 9 E 4 [30] CREA E4/ 4 <?,/ O H 9 E 4 [31] ', ' G AST ALT,CREA ALP^ N, VW _c 781 / 9, ST 4, ',,781 \] VW )_ 1 b cbco, D N,? \] R 2: [1] ;,,,. 7 8 > 1 LQ\c B C O 9 S T [J].,2001,21 (2): [2] V W. ]VW 781 BCO 9N c X [D]. :,2013. [3]. VW 1 b B C O c N S T9 [D]. " :,2008. [4] ZhaoX,LiL,LuoQ,etal.Efectsofmulberry ( 犕狅狉狌狊犪犾犫犪 L.)leafpolysaccharideson growth performance,diarrhea, bloodparameters,andgut microbiotaofearly weanlingpigs [J].LivestockScience,2015,177: [5]. X Y [ 9> [D]. :,2012. [6]. ( 9 +[D]. :,2011. [7] U,U,,. J b c 9ST[J].J:8,2012(4): [8], 0. VW > B C ` a 9 & ' [J].,2000 (10): [9]. J 9 [D]. :,2013. [10]. VWc > "# [D]. :,2016 [11],, a,. 1 c BC9ST[J].J:,2015(3): [12],,.! V W b BCO 9ST[J]. $*,2007,34(12): [13],,,. VW 781 G Y9ST[J].#$ Y,2008,29(1): [14],,,. C VW 781 b c D N 9 S T [J]. (@ G ),2006,32(6): [15],, L,.4 VW 781 b 9 ST[J]. J:,2007(12): [16] X c > 9 [D]. M :,2013. [17]. 1 X Y 9 S T c N[D]. :,2011. [18],,,. ) "# ( PRRSV 1 BC BJ9ST[J].,2015,35(10): [19].!BC [M]. :,2003: [20],,,.c Q 781 F" BC A Q \ 9 S T [J].!Z[,2008,20(2): [21],,. V W B C 9 c,[j].&,2013,30(4): [22],/01, #,. VW BCO 9ST

6 11 ', : VW 781 b cbco 9ST 37 [J]. (@G ),2013,42(5): [23] /.( 9 c B C S T X 9 [D]. :,2004. [24] MalekTR.Thelpercels,IL 2andthegenerationofcytotox ict celresponses [J].TrendsImmunology,2002,23(10): [25] Liao W,LinJX,Leonard WarrenJ.IL 2familycytokines: newinsightsintothecomplexrolesofil 2asabroadregula torofthelperceldiferentiation[j].currentopinioninim munity,2011,23: [26] SunP,WangJQ,ZhangHT.EfectsofBacilussubtilisnato, onperformanceandimmunefunctionofpreweaningcalves[j]. JournalofDairyScience,2010,93(12): [27] 0. " 8 b D 9ST[D].:,2013. [28] +,U ",U #,.$ %! b D 9ST[J].!Z [,2015,27(10): [29] &, ',,. 4VW 781 Nb BCb 9 S T [J].,2012,28(4): [30] \, (,,.+( C 781 b NBC 9ST[J].,2014(10): [31] 4). *+, 781 9= b > I V - 9 [D]../0 :./,2009. 犈犳犲犮狋狊狅犳犚犪犱犻狓狆狊犲狌犱狅狊狋犲犾犪狉犻犪犲犛狋犲犿犪狀犱犔犲犪犳犘狅犾狔狊犪犮犮犺犪狉犻犱犲犆狅犿犫犻狀犲犱狑犻狋犺犅犪犮犻犾狌狊犛狌犫狋犻犾犻狊狅狀犌狉狅狑狋犺犘犲狉犳狅狉犿犪狀犮犲, 犐犿犿狌狀犲犉狌狀犮狋犻狅狀犪狀犱犅犻狅犮犺犲犿犻犮犪犾犐狀犱犲狓犻狀犠犲犪狀犲犱犘犻犵犾犲狋狊 CAIXubin 1,CHEN Lingfeng 1,WU Xiaoqing 1,QINtao 1,LIJian 1, YU Daojin 1,LIN Guoxu 2,MA Yufang 1,HUANG Yifan 1 (1. 犓犲狔犔犪犫狅狉犪狋狅狉狔狅犳犜狉犪犱犻狋犻狅狀犪犾犆犺犻狀犲狊犲犞犲狋犲狉犻狀犪狉狔犕犲犱犻犮犻狀犲犪狀犱犃狀犻犿犪犾犎犲犪犾狋犺犻狀犉狌犼犻犪狀狆狉狅狏犻狀犮犲, 犉狌犼犻犪狀犃犵狉犻犮狌犾狋狌狉犲犪狀犱犉狅狉犲狊狋狉狔犝狀犻狏犲狉狊犻狋狔, 犉狌狕犺狅狌 , 犆犺犻狀犪 ; 2. 犘狌狋犻犪狀犢狅狌犾犻犽犲犃犵狉犻犮狌犾狋狌狉犲犪狀犱犃狀犻犿犪犾犎狌狊犫犪狀犱狉狔犇犲狏犲犾狅狆犻狀犵犆犗., 犔犜犇, 犘狌狋犻犪狀 , 犆犺犻狀犪 ) 犃犫狊狋狉犪犮狋 :ThisstudywasconductedtoinvestigatetheefectsofRadixpseudostelariaestemandleaf polysaccharide(rpslp)combinedwithbacilussubtilisongrowthperformance,immunefunctionandbio chemicalindexofweanedpiglets.onehundredandfifty25 day oldweanedpiglets(largewhite Land race)wereselectedandrandomlyalocatedintofivegroupsincludingcontrolgroup,antibioticgroup, groupi,iand I,withthreereplicatespergroupand10pigletsperreplicate.Thecontrolgroupofpig letswerefedabasaldiet,andothergroupswerefedabasaldietsupplementedwith100mg/kgtylosin,1 000mg/kgRPSLP,1000mg/kgbacilussubtilis,1000mg/kgRPSLPand1000mg/kgbacilussubtilis for30days.theresultsshowedasfolows:compared withthecontrolgroup,the ADGinantibiotic group,groupiand Ialincreased( 犘 <0.01),sodidgroup I( 犘 <0.05);thediarrhearateinalgroups decreased ( 犘 <0.01);InaspectsofIgA,IgGandIgM,thecontentsofIgAandIgMingroupI,Iin creased ( 犘 <0.05),thecontentofIgA,IgGandIgMingroup Iincreased ( 犘 <0.01);InaspectsofC3 andc4,thecontentsofc3andc4ingroupi, Iincreased( 犘 <0.01),thecontentsofC3andC4ingroup Iincreased ( 犘 <0.05);InaspectsofIL 2,IL 4andIFN γ,thecontentsofil 2,IL 4andIFN γingroup I,Iincreased( 犘 <0.05),thecontentsofIL 2,IL 4andIFN γingroup Iincreased( 犘 <0.01);thecon tentoftginfourexperimentalgroupssignificantlydecreased( 犘 <0.01);theALBcontentsinalgroups significantlyincreased( 犘 <0.01);theBUNinantibioticgroup,group Iand Isignificantlydecreased( 犘 <0.01)butdecreasedingroupI( 犘 <0.05).ItwasconcludedthatRPSLPcombinedwithbacilussubtilis indietscouldenhancegrowth performance,immunefunction andimprove biochemicalindexesinthe weanedpigletsandtheefectwasbeterthanaddedseparately. 犓犲狔狑狅狉犱狊 : 犚犪犱犻狓狆狊犲狌犱狅狊狋犲犾犪狉犻犪犲 stem andleafpolysaccharide; 犫犪犮犻犾狌狊狊狌犫狋犻犾犻狊 ;weanedpiglets; growthperformance;immuneindex;biochemicalindex