2016 8,, :, Akebiatrifoliate (Thunb).Koidz, 55,, 60, :, :, 2,4,6- (TPTZ):, E(Trolox):, ABTS:, DPPH:, :, 1.2 :SHB-ⅢA, :HH-S24S, :UV1800, :JRC-200

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1 32 8 OOD & MACHINERY 食品与机械 Vol.32,No.8 Aug.2016 DOI: /j.issn Comparativestudyonpolyphenol,flavonoidandantioxidantactivities fromdiferentpartsofakebia Trifoliata. 1,2 LU Jun 1,2 LUO Dan ZHANG Jia-qi 1 1 HUANG Hui 1 1 1,2 FU Chun-ya 1 LI Zhong-hai 1,2 (1., , ) (1.Colegeof Food Scienceand Engineering,CentralSouth Universityof Forestry and Technology,Changsha,Hunan410004, China2.NationalEngineering Laboratoryof Riceand By-product Deep Procesing,Changsha,Hunan410004,China) : 0.731)mg/g,respectively,andtheDPPH,ABTS,FRAPantioxi-, dantactivityvaluein CSJas ( ±5.462) μ moltrolox/g,, ( ±1.586) μ moltrolox/gand ( ±1.773) μ mol Trolox/g,respectively.Thepolyphenol,flavonoidscontentandan-, (TPC) (TFC) DPPH tioxidantactivityforalextractswereshowingthefolowingtenden- cy:cane>leaf> peel> seed > sarcocarp.spearmancorrelation (DPPH) ABTS (ABTS) studyshowsthatthecontentofpolyphenol,flavonoidsandstrongly (FRAP) (63.033±0.482)mg/g, relatedtotheantioxidantactivity.theresearchresultsprovideex- (26.395±0.731)mg/g,( ±5.462) μ moltrolox/g, perimentalbasisfordeepprocessingofakebia Trifoliata. ( ±1.586) μ moltrolox/g,( ±1.773) μ mol Keywords:Akebia Trifoliata.antioxidantpolyphenolflavonoid Trolox/g : > > > > (Akebiatrifoliate(Thunb).Koidz) Spearman,, [1], 50%, VC 15, :,,, Abstract:Inordertoevaluatethecontentofpolyphenolandfla- [2-3],, vonoid,aswelasantioxidantactivityindiferentpartsofakebiatri-, foliata.,theultrasonic-assistedandreflux method wereused.the,, contentofpolyphenolandflavonoids,andantioxidantcapacityindif- ferentpartswerecompared.theresultsshowedthattheultrasonic extraction method was more eficientthan thereflux extraction, 40%,, [4] method,amongthealextracts,thehighestcontentofpolyphenol,, andflavonoid,andstrongestantioxidantcapacitywasfoundincane, extractbyultrasonic-assited (CSJ).Thecontentsofthetotalpoly- [5], phenol,flavonoidin CSJas (63.033±0.482)mg/g,(26.395± [6], : ( :2015NK3022) [7-8] :,,, [9-10] [11][12]30-40[13]17-27 : (1960 ),,,, lizh11@163.com [13]37-42 : , 132

2 2016 8,, :, Akebiatrifoliate (Thunb).Koidz, 55,, 60, :, :, 2,4,6- (TPTZ):, E(Trolox):, ABTS:, DPPH:, :, 1.2 :SHB-ⅢA, :HH-S24S, :UV1800, :JRC-2000, , [14-15] (1) : 1g ( ), 50mL70% h, 70% 50mL,-4 (2) : 1g ( ), 50mL70% min, 45min, 70% 50mL, [16] [17] (1)DPPH : [18] (2)ABTS : [19] (3) : [20] , DPPH FRAP ABTS 2.2, R ~ Figure1 Thestandardcurve , 133

3 第３２卷第８期陆俊等三叶木通不同部位多酚黄酮含量及抗氧化活性比较Ｔｘｇ±１Ｔｘｇ约是果肉的１３５倍由５８７μｍ此说明抗氧化效果与不同部位的提取液具有一定的关系虽然三种方法测定出来的数值不一样但其趋势都是一致的其抗氧化能力均为藤茎叶片果皮种子果肉３三叶木通不同部位回流提物多酚黄酮含量由图４可知三叶木通的各部位加热回流法的提取物均含有黄酮和多酚等有效成分且差异较大提取物中有效成分仍是多酚居ｇ黄酮较少ｇ与多４９９１５２４０ｍｇ２３５２６１０８９ｍｇ超声波提取物所得结论一致各部位多酚含量趋势为藤茎叶片果皮种子果肉藤茎的多酚含量最多图２三叶木通不同部位超声波辅助提取物多酚黄酮含量１９９ｍｇｇ相当于含量最少的果肉的４９９１５ｍｇｇ±０２Ｔｈｐｎｄｖｎｄｎｎｙｐｈｎ２２倍各部位黄酮含量大小顺序与多酚含量大小顺序一ｄｘｍｄｎｐｕｎ致含量最高的是藤茎３１４ｍｇｇ含量最２３５２６ｍｇｇ±０Ａｋ少的是果肉１０８９ｍｇｇ±００１２５ｍｇｇ各部位除叶片和的超声波法提取物均含有黄酮和多酚等有效成分且差异较大其中多酚含量较多６３０３３３５１０ｍｇｇ而黄酮含量相果皮外其它部位间均有显著性差异在所有提取物中超声波提取物的多酚黄酮含量均高于热回流提取物效率较高对较少２６３９５０７３１ｍｇｇ各部位多酚含量大小顺序为藤茎叶片果皮种子果肉各部位间均有显著差异其中藤茎的多酚含量已达到６３０３３±０４８２ｍｇｇ相当于含量最少的果肉３５１０ｍｇｇ±００２５ｍｇｇ的１８倍各部位黄酮含量大小顺序与多酚含量大小顺序一致除藤茎和叶片之间无显著差异外其它部位之间均有显著性差异其中藤茎的黄酮含量为２６３９５±０７３１ｍｇｇ相当于含量最少的果肉黄酮含量０２２ｍｇｇ的１０倍０９７７ｍｇｇ±０２三叶木通不同部位超声波提取物抗氧化活性由图３可知三叶木通各部位超声波法提取物抗氧化活性差异较大其中ＤＰＰＨ法测得抗氧化活性最强的是藤茎图４三叶木通不同部位热回流提取物多酚黄酮含量３８０８６０μｍＴｘｇ±５Ｔｘｇ其次是叶４６２μｍ片抗氧化活性最差的是果肉部分藤茎的抗氧化活性约是果肉的３９倍ＲＡＰ法测得抗氧化活性大小的顺序为藤茎叶片果皮种子果肉该法测得各部位之间抗氧化活性差异均显著藤茎的抗氧化活性仍最强３８７４０６ μｍ７７４μｍＴｘｇ±１Ｔｘｇ约是果肉的６６倍各部位提物对ＡＢＴＳ自由基也具有清除活性且不同部位之间均存在着显著差异藤茎的清除能力仍为最强３３７１８１μｍ４ＴｈｐｎｄｖｎｄｎｎｕｘｙｐｈｎｘｍｄｎｐＡｋ４三叶木通不同部位回流提物抗氧化活性由图５可知三叶木通各部位的热回流法提取物的抗氧化活性存在显著差异ＤＰＰＨ法测得的抗氧化活性最强的仍是藤茎５７５μｍ３０８２８５μｍＴｘｇ±３Ｔｘｇ明显高于其它部位其次是叶片果肉的最差ＲＡＰ法测得抗氧化活性大小的顺序为藤茎叶片果皮种子果肉藤茎的抗氧化活性３５６０２８μｍＴｘｇ± ２２５μｍＴｘｇ约是果肉的２１倍各部位提物对ＡＢＴＳ自由基也具有清除活性能力最好的仍为藤茎２５６３６ μｍＴｘｇ± １８６７μｍＴｘｇ能力最差的是果肉１３４２２ μｍ０９２μｍＴｘｇ各部位抗氧化能力大小Ｔｘｇ±０顺序与超声波提取物的抗氧化能力大小顺序一致且三种方法所得出的趋势也一致其抗氧化能力均为藤茎叶片果皮种子果肉综上所述超声波法对三叶木通多酚和黄酮的提取效率要大于热回流法这可能与超声波的空化作用有利于活性成图３三叶木通不同部位超声波辅助提取物抗氧化活性分的释放有关这和成晓霞等１５研究发现超声波对三叶木３Ｔｈｎｘｄｎｖｕｎｄｘｙ通常春藤皂苷的提取得率要高于热回流法结论一致不同ｍｄｎｐＡｋ１３４部位的提取物中以藤茎的多酚和黄酮含量最高抗氧化能

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第期李伟等用方法对中国历史气温数据插值可行性讨论

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