2003 www.influenza.org.cn 1
...5...6 1....7 1....9...9 2....9...10...10 3. 1 2...11...12...13...14...15...16...16...18 4. 3...18...19...19...20...20 5. 4...21...21...23 6....23 1...24 2...24 3...25 4...25...26...27 7....27 I...28 II...28 III BSC...31 BSC...32 BSC...32 BSC...33 8....35...35 9....36...38 www.influenza.org.cn 2
www.influenza.org.cn 3...38...39...39...39...39...41 10....41...41...41...42...42...43...43...43...44...44...45...45...45...45...47 11. DNA...48...48 DNA...48...49...49...49...50 12....50 13....50...51...51 14....53...53...54...54...57 BSC...57...58...58...59...60...60...60
www.influenza.org.cn 4...61 15....61...61...61...61...63...63...65...65 16....66 17....67...67 18....67...68...69...69 19....69...70...70...70 20....71 GLP...72 1 GMT...73 2...73 3 GLP...74 4 GLP...75 5 3 4 GLP...75...76 21....76...76...77...77...77...77...78...78...78...79...79...79...79...80...80...81
...81 1...81 2...82 1983 2003 WHO DNA WHO W. Emmett Barkley, Howard Hughes Chevy Chase www.influenza.org.cn 5
Dr Ingegerd Kallings Srockholm Mary Ellen Kennedy Ashton,Ontario, Richard Knudsen GA Nicoletta Previsani Geneva Jonathan Richmond GA Syed A. Sattar Deborah E. Wilson Riccardo Wittek Lausanne Lausanne www.influenza.org.cn 6
1. WHO 1,2,3,4 1 2 3 4 1 2 3 4 1. 2. 1. GMT 1 2. GMT 2 3. 2 BSC 3 4. 3 III BSC 4 II BSC www.influenza.org.cn 7
BSC GMT 3. 1 2 3 4 1 2 3 4 HEPA 1 2 3 BSC I www.influenza.org.cn 8
II III CDC NIH 1 2 1. 2. www.influenza.org.cn 9
www.influenza.org.cn 10 3-2 (1) (2) (3) GMMOs GMMOs DNA
integrins GMMO GMMO GMMO GMMO GMMOs 11 4 5 3. 1 2 1 4 www.influenza.org.cn 11
1 2 3 1 2 3 4. 1. 1 2 2. 3. 4. 16 5. 6. 7. 1. 2. 3. 4. 5. 6. 7. www.influenza.org.cn 12
8. 9. 1. 2. 3. 4. 5. 6. 1. 2. 3. 4. 5. 1. 2. 3. 4. 5. 6. www.influenza.org.cn 13
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 1 2 16. 17. 18. 19. 20. www.influenza.org.cn 14
1. 2. 3. 4. 5. 6. 7. 1 www.influenza.org.cn 15
2 1. 2. 3. 1. 2. www.influenza.org.cn 16
3. 14 ph 1. 2. 3. 4. 5. www.influenza.org.cn 17
14 14 4. 3 3 2 3 1 2 3 1 2 3 1 2 www.influenza.org.cn 18
1 2 1. 2. 3. 4. 3 3 2 1 2 1. 2 2. 3. 4. 5. 6. 7. 8. 9. 10. HEPA HEPA 11. 7 12. I II HEPA www.influenza.org.cn 19
HEPA 13. 14. 15. 2 3 I II III 3 1 2 3 1. 3 2. 3. 3 4. 3 2 5. 3 A www.influenza.org.cn 20
1 2 B 5. 4 4 4 3 4 1. www.influenza.org.cn 21
III III III 7 4 III HEPA 2. 4 3. HEPA III III III HEPA HEPA III HEPA HEPA HEPA HEPA 4 HEPA HEPA 4. III www.influenza.org.cn 22
ph 5. 6. 4 6. 4 1 ABSL 1 2 ABSL 2 ABSL 1 I II BSC 3 ABSL 3 ABSL 2 BSC 4 ABSL 4 ABSL 3 III BSC www.influenza.org.cn 23
ABSL BSC 1 2 3 4 4 1 1 2 2 1. 1 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 14 12. I II HEPA 13. 14. www.influenza.org.cn 24
15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 3 3 1. 1 2 2. 3. 4. 5. HEPA 6. 7. 8. 3 9. 10. 11. 12. 4 4 5 1. 1 2 3 www.influenza.org.cn 25
2. 3. 4. 5. 4 4 6. 7. HEPA 8. 9. 10. 11. 12. 4 4 13. 14. 15. aschelminthes 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. www.influenza.org.cn 26
www.influenza.org.cn 27 13. 1 6 9 7. 5µm 5 10µm BSC BSC 5 BSC BSC 1 3 I II III 4 III 4 I II II III IIB1 IIA2 I IIB2 III BSC HEPA HEPA 99.97 0.3µm 99.99 HEPA BSC HEPA BSC 5
I 3.I A B C HEPA D 3 I BSC 0.38m/s BSC HEPA a b c HEPA BSC I BSC I BSC BSC II II BSC www.influenza.org.cn 28
II BSC A1 A2 B1 B2 HEPA I II BSC 2 3 II BSC 4 II A1 BSC 4.IIA1 BSC A B C HEPA D E HEPA F II A BSC 4 0.38m/s HEPA 6 18cm 70 HEPA 30 II A1 BSC BSC 5 www.influenza.org.cn 29
II A2 BSC B1 B2 BSC IIA2 IIB1 5 IIB2 BSC IIA1 6 I III BSC BSC 5 BSC IIA IIB BSC 10 11 5.IIB1 BSC A B C HEPA D HEPA E F G HEPA 6.I II III BSC BSC m/s www.influenza.org.cn 30
I a 0.36 0 100 IIA1 0.38 0.51 70 30 IIA2 a 0.51 70 30 IIB1 a 0.51 30 70 IIB2 a 0.51 0 100 III a NA b 0 100 a b NA III BSC 6 BSC 4 HEPA HEPA 124.5Pa III BSC HEPA III BSC III BSC 3 4 www.influenza.org.cn 31
6.III BSC A B C HEPA D HEPA E F BSC IIA1 IIA2 2.5cm BSC IIB1 IIB2BSC BSC BSC BSC BSC 1 4 www.influenza.org.cn 32
5 BSC BSC I IIA1 IIA2BSC IIB1 BSC IIB2 BSC BSC BSC 0.45m/s BSC 30cm 30 35cm BSC BSC BSC 1 II BSC 70 BSC 24 IIA1 IIA2 BSC IIB1 IIB2 BSC 5 BSC BSC BSC www.influenza.org.cn 33
BSC BSC BSC HEPA / BSC BSC 5 BSC BSC BSC 1 2 3 4 BSC BSC BSC www.influenza.org.cn 34
BSC 1 10 19 8. 9 7 7. * * * * BSC * BSC * 30 BSC www.influenza.org.cn 35
HEPA BSC BSC O 30 BSC BSC O Warburg 9. BSC 7 www.influenza.org.cn 36
www.influenza.org.cn 37 8 10 8. I BSC II BSC III BSC 0.45vm
HEPA HEPA 3 4 BSC BSC www.influenza.org.cn 38
3 BSC BSC BSC Bunsen 3 BSC 9 9. www.influenza.org.cn 39
BSC HEPA BSC 12 20 21 www.influenza.org.cn 40
10. 12 BSC 1. 2. 3. 4. 5. www.influenza.org.cn 41
6. 7. 18 24 8. BSC 9. 10. 1. 2 3mm 6cm 2. Bunsen 3. 4. 5. 12 1. BSC 2. BSC 3. BSC 4. BSC 5. BSC Bunsen 6. 7. 8. www.influenza.org.cn 42
9. 10. BSC 11. 5 5 BSC BSC 7 1. 2. 3. 4. 5. 1. 2. 3. a b 4. 5. 1. 2. 3. 4. 18 5. 6. www.influenza.org.cn 43
1. 2. 3. 4. 5. 6. BSC 7. 8. 9. 10. 3 4 11. 12. 13. 14. 15. 16. I II BSC III BSC 1. 2. 3. PTFE 4. BSC 5. BSC 6. www.influenza.org.cn 44
1. PTFE 2. BSC 1. BSC 2. 3. 4. BSC 1. 2. 3. 4. 5. 6. www.influenza.org.cn 45
1. 1 2 3 4 12 5 2. 1 2 2 3 3. 1 I II BSC 2 3 4 4. 1 2 5. 6. 1 2 3 7. 1 2 20 o C 8. 1g/l 10g/l www.influenza.org.cn 46
TSEs Creutzfeldt-Jakob CJD Gerstmann-Str.ussler-Scheinker BSE CJD TSEs BSC 1. 2. 3. 4. 5. BSC 6. 7. 8. 9. 10. 2mol/l 1 11. BSC 12. BSC 2mol/l HEPA 13. 2mol/l 1 14. 2mol/l 1 12 22 23 www.influenza.org.cn 47
11. DNA DNA GMOs 1975 Asilomar DNA 25 DNA DNA DNA DNA DNA GMOs DNA GMO puc18 K12 puc18 pbr322 K12 K12 K12/pUC18 1 DNA DNA DNA DNA www.influenza.org.cn 48
www.influenza.org.cn 49 DNA DNA DNA
DNA DNA GMOs GMOs 25 27 12. 2002 12 2002 12 2002 12 2003 WHO 13. 3 4 3 4 www.influenza.org.cn 50
1. 2. 3. BSC 1 24 4. www.influenza.org.cn 51
30 24 5. 30 30 24 60 6. BSC 7. 8. 9. / www.influenza.org.cn 52
www.influenza.org.cn 53 10. 3 4 12 28 14.
a b / w/v 10 10. 0.1 1g/l 0.5 5g/l 5% 20ml/l 100ml/l 70 1.4g/l 7.0g/l 60 1.7g/l 8.5g/l 1 /l 4 /l 1.5g 2520g/l 20g/l www.influenza.org.cn 54
NaOCl 1g/l 5g/l 5g/l Hanta 50g/l 1:50 1:10 1g/l 5g/l 120g/l 70 1.4g/l 7.0g/l 1.0g/l 5g/l 2mg/l NaDCC 60 NaDCC 1.7g/l 8.5g/l 1.0g/l 5g/l NaDCC 1.5g 4 1.0g/l 5g/l NaDCC NaDCC 10 25 20g/l 1 2g/l HCl NaClO2 20 o C 70 370g/l 100ml/l BSC www.influenza.org.cn 55
OHC CH2 3CHO 20g/l 2 1 4 triclosan Triclosan triclosan C2H5OH 2- CH3 2CHOH 70 70 v/v 100g/l 2g/l 70 v/v BSC 10s 3 www.influenza.org.cn 56
/ 4 10 o C H2O2 3 5 5 10 3 6 BSC / / CH3COOOH NaOCl 1g/l 5g/l 3 H2O2 21 o C 70 BSC 8 BSC 0.8 BSC I II BSC BSC www.influenza.org.cn 57
10 BSC 70 BSC 1 BSC BSC 1 10s 160 o C 2 4 134 o C 3 126 o C 10 121 o C 15 115 o C 25 1 7 HEPA 2 HEPA www.influenza.org.cn 58
3 134 o C 3 80 o C 7. www.influenza.org.cn 59
800 o C 1000 o C 3 / Creutzfeldt-Jakob 2mol/l NaOH 4.0mol/l HNC NH2 2HCl NH2 2HNCO NaOCl >2 132C 4.5 10 / www.influenza.org.cn 60
15. MSDS 1 2 3 4 www.influenza.org.cn 61
www.influenza.org.cn 62
www.influenza.org.cn 63 11. 2-
O M 1,2- O 1,3 P 1,4 www.influenza.org.cn 64
Na2CO3 NaHCO3 1. 2. 3. 4. 5. 6. www.influenza.org.cn 65
41 45 16. H2O CO2 12 12. a www.influenza.org.cn 66
42 17. CO2 12 46 48 18. www.influenza.org.cn 67
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. www.influenza.org.cn 68
1 2 19. www.influenza.org.cn 69
BSC 3 4 3 BSL4 1. 2. 3. 4. 5. 6. 7. www.influenza.org.cn 70
www.influenza.org.cn 71 8. 9. 20. / / /
GLP 1. 2. 3. 1. 2. 3. 1. 2. 3. BSC 4. 5. 1. 2. 3. 1. 2. 3. 4. 1. 2. www.influenza.org.cn 72
3. 1 GMT BSL-1 BSL-2 3 1. 2. 3. 4. 5. 6. 7. BSC I II III BSC 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 2 2 1. 2. 3. 4. BSC 5. www.influenza.org.cn 73
6. 7. 1. 2. 3. 4. 5. 6. 7. 8. 3 GLP 1. 2. 3. 4. 5. 1 2 6. BSC 2 7. 3 8. 9. / 10. www.influenza.org.cn 74
4 GLP 5 1. 2. 3. 4. 5. 6. 7. 8. 9. HEPA 10. 11. BSC 12. 13. 14. 15. 16. 17. 18. 19. 20. ; 21. 22. 5 3 4 GLP 3 4 4 www.influenza.org.cn 75
3 4 1. 3 4 2. 3 4 3. 3 4 4. I II BSC BSL-3 3 5. III BSC BSL-4 6. 7. III BSC 8. 9. 10. 49 51 21. 1. 2. 3. 4. 5. 6. 4 7. 8. 9. 10. 11. www.influenza.org.cn 76
12. 13. 14. 15. 1. 2. 1. 2. 3. WC 4. 5. 6. 7. 8. 9. 1. 2. 3. 4. HEPA 5. BSC 1. 300 400lx 2. 3. www.influenza.org.cn 77
4. 5. 1. 2. 3. 4. 5. 1. 2. 3. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. www.influenza.org.cn 78
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 1. 2. 3. 4. 5. 6. 1. 2. 3. 4. 5. 6. 7. 1. www.influenza.org.cn 79
2. 3. 4. 5. 6. 7. 8. 9. HEPA 10. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 1. 2. 3. BSC www.influenza.org.cn 80
4. 5. 6. 7. 8. 9. 1. 2. 3. BSC 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. BSL-3 BSL-4 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 1 www.influenza.org.cn 81
1. B BCG WHO BSC WHO 652 BCG Geneva WHO 1999 WHO/V&B/99.23 2. A B A 3 3 2 WHO * WHO Lyon CSR Lyon Devourg 58 69007 http://www.who.int/csr/ *WHO Novle V.g18 S-171 82 Solna http://www.smittskyddsinstitutet.se/english/english.htm *WHO Tunney Pasture 0700A1 Ontario K1A 0L2 http://www.hc-sc.gc.ca/hpb/lcdc/biosafty *WHO Mailstop F05 Clifton 1600 30333 http://www.cdc.gov/ *WHO MD20892-5760 Bethesda MSC 5760 13/3K04 13South Drive http://www.nih.gov/ *WHO Wreckyn 10 Nth Melbourne 3051 Locked Bag815 PO Carlton Sth 3053 http://www.vidrl.org.au/ www.influenza.org.cn 82