,2018,38(11): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2018) 犱狅犻 : /j.issn "#$%& 犆犻犘犝犅 22 ' (,,,,,,!

,2018,38(11):1961-1967 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. :1000 4025(2018)11 1961 07 犱狅犻 :10.7606/j.issn.1000 4025.2018.11.1961 "#$%& 犆犻犘犝犅 22 ' (,,,,,,! (456 7 8, 9: ;-./, 9 < = >? @ A -./BC DE, 010018) ) *: FGH( 犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪 )IJ IK ILM,NOJ ),PQRSC 9 :TU,VW XY TU TU? ' FGHOJ Z[\$] ^_1` 犆犻犘犝犅 22 (plant U box22)tu cdna abc, 犆犻犘犝犅 22TU 1260bp, 419 T PCR,' L ABA ;1h 犆犻犘犝犅 22TU _,. X 12 35 7,OJ ; 12d _,X 2.5, 犆犻犘犝犅 22 Z[ 犆犻犘犝犅 22TU Z,, ZTU 9.,'150mmol/LNaCl 1μmol/LABA 400mmol/L ;,, 犆犻犘犝犅 22TU ' / L I +,-:E3 ; FGH; 犆犻犘犝犅 22; / ; ". /:Q786;Q789 01 :A 犆犾狅狀犻狀犵犪狀犱犉狌狀犮狋犻狅狀犪犾犆犺犪狉犪犮狋犲狉犻狕犪狋犻狅狀狅犳犆犻犘犝犅 22 犳狉狅犿犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪 YUE Wenran,YU Xiumin,YUEJunyan,YANG Qi, HAN Xiaodong,WANG Ruigang,LIGuojing (ColegeofLifeSciences,InnerMongoliaAgriculturalUniversity,InnerMongoliaKeyLaboratoryofPlantStressPhysiologyand MolecularBiology,Inner MongoliaScientificInnovation Team ofgeneticresource Utilizationand MolecularImprovementof StressResistantPlants,Hohhot010018,China) 犃犫狊狋狉犪犮狋 : 犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪 isanimportantsand fixingshrubinnorthwestaridareawithdrought,cold andsaline alkaliresistance.excelentstressresistantcandidategenesscreenedfromitcouldprovidegood generesourcesforgeneticengineeringofforestandforagespecies.inthisstudy,thefullengthcdnase quenceof 犆犻犘犝犅 22(plantU box22)hadbeenminedfromthetranscriptomelibraryconstructedinourla boratoryusingdroughtstressedseedlingsof 犆. 犻狀狋犲狉犿犲犱犻犪.The 犆犻犘犝犅 22genecontained1260bpopen readingframe(orf)whichencoding419aminoacids.quantitativerealtimepcranalysisshowedthat theexpressionlevelsof 犆犻犘犝犅 22weresignificantlyincreasedandreachedthehighestlevel,whichwas12, 35and7timesofthecontrolat1hafterdehydration,NaCland ABAtreatments,respectively.After droughttreatment,therelativeexpressionof 犆犻犘犝犅 22wassignificantlyincreasedandreachedthehighest levelat12d,whichwas2.5timesmorethanthatofthecontrol.theresultsindicatedthatthetranscrip :2018 08 18; :2018 11 13 :!"(KCBJ2018012)!:#$%(1990-),&,',()*+,-./ E mail:yuewenran@126.com 01 :,, 2, 3,()*+,-./ E mail:liguojing@imau.edu.cn

1962 38 tionof 犆犻犘犝犅 22wasinducedbyabioticstress.Furthermore,expressionvectorpCanG 犆犻犘犝犅 22wascon structedandtransformedintothe wild type 犃狉犪犫犻犱狅狆狊犻狊.Homozygoustransgenic 犃狉犪犫犻犱狅狆狊犻狊 overex pressing 犆犻犘犝犅 22 weregeneratedforfurtheranalysis.seedgerminationassayshowedthat 犆犻犘犝犅 22 transgeniclinesgerminatedslowerthanthatofwild typeon mediumcontaining150 mmol/l NaCl,400 mmol/l mannitolor1μmol/l ABA.Theseresultsindicatedthat 犆犻犘犝犅 22decreasedtoleranceofthe transgenic 犃狉犪犫犻犱狅狆狊犻狊 tosaltandosmotictreatmentsduringseedgermination. 犓犲狔狑狅狉犱狊 :E3ubiquitinligase; 犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪 ; 犆犻犘犝犅 22;seedgermination;osmoticstress /26S (ubiquitinproteasome pathway,upp)n ), Q [1], N ), :,' ATP ',,,;,26S, [2] UPP ( ) (Ub), (E1) (E2) (E3) 26S \,R, E3 ' Q!_() A [3] E3 () N" )#,,$, % & ' (Lys),* Z _ ', E3 T U 1400 [4], 1300 [5] "()*+, E3-. 5 [6] :HECT (homologoustoe6 APC terminus) RING (realyinterestingnew gene)/u box APC (anaphasepromotingcomplex,. / 0 ) SCF (Skpl/CulinorCDC53/F boxprotein)0 CUL3 BTB U box N 1 E3, 1* UFD2(ubiquitinfusiondeg radationprotein2), 70 2 T,N 3 4 b U / [7] U box N B 5 RING,R 2\ 67,0./ A89 :; / "(,' 64 T U U box E3 [8], )* 77 [9] ), U box E3 - b <=>?@A [10] %BC,) D? 犘犝犅 22 R E? T U 犘犝犅 23 2 E? U box E3 PUB22 PUB23- RPN12a VW, RPN12a, C O J < = [11] ( 犆犪狆狊犻犮狌犿犪狀狀狌狌犿 )CaPUB1 (putative U box protein1),- PUB22 PUB23E?. X 51% - 52%,,CaPUB1 - RPN6,0, RPN6 C OJ L <= [12] SeoD H > [13] 犘犝犅 22 犘犝犅 23' O J < = N F G ' ABA,HNR IFJK ChoSK > [14] PUB22 PUB23, RNP6 ZhaoJF> [15], PUB22 PUB23 0, ABA PYL9 (pyrabactinresistance like)l OJ I M N O,PUB22 PUB23 I - PAMP (pathogen associatedmolecularpaterns)1 [16] MPK3 (mitogen activatedproteinki nase3)! <= [17] F G H ( 犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪 ); PQ FGH; B RS,T IJ I K I U ILM>,NOJ V WX Y SC ;MNO, FGHI A A, N? 5 Z [ [18 19] \ ' F G H O J Z [ \ $ ] ^ _ 1 ` E3 T U a b cdnac, 1]. R; U box E3 PUB22 @ A PCR ^ 犆犻犘犝犅 22' O J L ABA > ;. 0, 犆犻犘犝犅 22', Z T U ' / L I _. X ` 犆犻犘犝犅 22 TU' FGH<=9: a Tb 1 Z[ c 1.1 2345678 FGH / /, (1 2), 22 16h /8h U Q1 b NaCl ABA ; *,,. 300mmol/LNaCl 100μmol/LABA

11. #$%,>: FGH 犆犻犘犝犅 22TU -. 1963 ;,. ' ; 0 1 3 6 12 24 48h, 3.,,-80 ; 3 0 OJ ;: b1, 1d X,O, 3d 1,OJ16d 0,0 2d,. X0 3 6 9 12 15 17d (16F), c E ( 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 )Colum bia 0 (Col 0) \, pcang HA 8<=-,6 DH5a 4 GV3101 \ 1.2 7 8 1.2.1 9 犚犖犃 : ; @ A Trizol FGH RNA A ( Quawel. Q5000) RNA,A RNA, Q RNA cdna 3, (a :,AT311 03) 1.2.2 <56=>?@ ( Z[\ ] TUc,@A Primer5 F 犆犻犘犝犅 22 Q (TCAGAAAAGGTAACAA AAGCGACA) R 犆犻犘犝犅 22 Q (TTCAGCTCT TCCCTCAGCACA) A SYBR GreenⅠ [,@A LightCycler480 PCR (Roche ) = X:10 μl SYBRPremixExTaq(TaKaRa ), 0.4μL(10μmol/L), 5μL,DEPC 4.2μL, 20μL = cx:95 30s,95 5s,60 15s,72 4 30 s,40, A2 -ΔΔCt c A X q 犆犻犈犉 1α F (CAAAAAGTC CCCTCGTTGTCTC) q 犆犻犈犉 1α R (AGCAATC GTTCTTCCTAATGATCTAA );q 犃狋犈犉 1α F (AGAA GGGTGCCAAATGATGAG ) q 犃狋犈犉 1α R (GGA GGGAGAGAGAAAGTCACAGA) 1.2.3 '?@ABCD W FGH 1 cdna X, pcang HA, 犛犪犾 Ⅰ 犛犪犮 Ⅰ & F 犆犻犘犝犅 22 (GCGTCGACAT GGATGAGATCGATGTTCCT, X 犛犪犾 Ⅰ ) R 犆犻犘犝犅 22(GCGAGCTCTTTCACA CATAATTAGGGTACGA, X 犛犪犮 Ⅰ ) PCR, ` X:98 3 min,98 15s,57 30s,72 4 1 min,30 ;72!410min @A "# ($ )"#" %&, ' peasy BluntSimple ( a : ),Z,6 DH5a, R PCR \ /, c, ' pcang HA, R PCR, \ Z,4 GV3101,( ) PCR - c,\ A c * ^+, -. 1.2.4 E 0 /P \ pcang 犆犻犘犝犅 22Z, [20],0 # / ' 25mg/L 1 1/2MS T PQ),P Q _ T 3 RNA, R Z[ cdna,@a PCR ZTU,Q 3 1.2.5 <56= FGHIJK / A75% 2 10 min, A 3 2 10 min,4 / 5 O. ' 150 mmol/l NaCl 1μmol/L ABA 400 mmol/l 1/2MS T, 556 /,4 E, ;3d,, 22 16h /8h U,24h 7 2 -. 2.1 犆犻犘犝犅 22 '> L?@ABCD. 89 FGHOJZ[\$] E3 TU,T.9. UTR c UN, pcang HA 犛犪犾 Ⅰ 犛犪犮 Ⅰ & F 犆犻犘犝犅 22 R 犆犻犘犝犅 22,@ A F G H cdna X, PCR, _" %&(: 1,A) _ c % & ' peasy Blunt Simple, c c A 犛犪犾 Ⅰ 犛犪犮 Ⅰ ' pcang HA, \ pcang 犆犻犘犝犅 22 Z, 6,( ) R,A 犛犪犾 Ⅰ 犛犪犮 Ⅰ (:1,B), %&, \ \ Z, 4 GV3101,( ) PCR, ;:1,C 9

1964 38 2.2 犆犻犘犝犅 22 'MN ( _ 犆犻犘犝犅 22 T U cdna c, 1260bp,!7 / ATG, / TGA, 419 T (:2) T c ' NCBI BlastP, RT U box,- ( 犕犲犱犻犮犪犵狅狋狉狌狀犮犪狋狌犾犪 )PUB23 (XP_013450220.1) < ( 犆犻犮犲狉犪狉犻犲狋犻狀狌犿 ) PUB22 (XP_004494254.1) ( 犆犪犼犪狀狌狊犮犪 犼犪狀 )PUB22 (XP_020225250.1) PUB22 (NP_190813.1) PUB23 (OAP08906.1)=>. X84% 84% 81% 58% 58%, _ T U ; U box T U,U N R 7? X 犆犻犘犝犅 22 @A MEGA5 @ % =,A (: 3),CiPUB22 - PUB23 < PUB22 B _!,c 89 c =>. X84%,CD? % 2.3 犆犻犘犝犅 22 '?@ ( @A PCR 犆犻犘犝犅 22TU'FE ;.,OJ ;12d 犆犻犘犝犅 22TU _, XE ; 2.5,15d (: 4,A) ;1h 犆犻犘犝犅 22 _. 9 / :2 犆犻犘犝犅 22TUcDNA c T c.indicatesterminationcodon Fig.2 ThecDNAanddeducedaminoacid sequencesof 犆犻犘犝犅 22 A. 犆犻犘犝犅 22,M.DL10000;1 2. 犆犻犘犝犅 22cDNA;B.pCanG 犆犻犘犝犅 22 \ ; 1. \ ;2. 犛犪犾 Ⅰ 犛犪犮 Ⅰ ;M.DL5000;C.pCanG 犆犻犘犝犅 22 R PCR ;1~4. ;M.DL5000 :1 pcang 犆犻犘犝犅 22 A. 犆犻犘犝犅 22,M.DL10000;1,2. 犆犻犘犝犅 22cDNA.B.IdentificationofpCanG 犆犻犘犝犅 22digestedbyrestrictionenzyme. 1.Vectorcontrol;2.Digestwith 犛犪犾 Ⅰ and 犛犪犮 Ⅰ;M.DL5000;C.IdentificationofpCanG 犆犻犘犝犅 22bycolonyPCR; 1-4.Randomsinglecolony;M.DL5000 Fig.1 TheconstructionofpCanG 犆犻犘犝犅 22recombinantvector :3 CiPUB22-R =?,. Fig.3 PhylogeneticanalysisofCiPUB22andotherrelatedproteins

11. #$%,>: FGH 犆犻犘犝犅 22TU -. 1965, _E ; 12 F,3h 7,12h, E ;,24hT \G0_E ; ' NaCl ;, 犆犻犘犝犅 22TU'1h XE ; 35 F,3h H I ' ABA ;1h, 犆犻犘犝犅 22TU XE ; 7,H3h,T\-E ;,6h E ; (: 4,B) J K 犆犻犘犝犅 22 Z[,L 犆犻犘犝犅 22 V - F G H M./ 2.4 犆犻犘犝犅 22 'O B?@ ( \ pcang 犆犻犘犝犅 22Z',P Q, T 1 T 3 ) 8, JK RNA, Z[ cdna,@atu & F 犆犻犘犝犅 22 R 犆犻 犘犝犅 22 PCR +,; : 5,A 9,W cdna X N," `Y, FGH 8 ZTU," `Y, 犆犻犘犝犅 22T U ' Z T U,@ A qrt PCR 8 ZTU,;: 5,B 9,Q 3 T 3 6 T 3 19 T 3 24. 2.5 犆犻犘犝犅 22 'E FGHIJK 3 /,. ' 0 150 mmol/l NaCl 1μmol/L ABA 400mmol/L 1/2MS T, / O '1/2MS T,3 - (: 6,A E),1μmol/L ABA ;4$, X 97%,3. X90% 75% 82%(:6, B F) ' 150mmol/LNaCl T, 5$ X94%,3. X 86% 61% 66% (: 6,C G) '400mmol/L ;,3 (:6,D H) W :&? 犆犻犘犝犅 22 T U / & L ABA I 8 :4 qrt PCR ;` 犆犻犘犝犅 22TU Fig.4 Theexpressionof 犆犻犘犝犅 22underdiferenttreatmentsdetectedbyqRT PCR A. 犆犻犘犝犅 22ZTU RT PCR + :M.DL2000;C -. ( cdna);c +.) ( FGHcDNA); B.qRT PCR + 犆犻犘犝犅 22ZTU :5 犆犻犘犝犅 22ZTU - A.RT PCRidentificationof 犆犻犘犝犅 22transgenic 犃狉犪犫犻犱狅狆狊犻狊 lines:m.dl2000;c -.Negativecontrol( 犃狉犪犫犻犱狅狆狊犻狊 cdna); C +.Positivecontrol( 犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪 cdna);b.qrt PCRanalysisof 犆犻犘犝犅 22expressionin 犆犻犘犝犅 22transgenic 犃狉犪犫犻犱狅狆狊犻狊 lines Fig.5 Identificationandexpressionanalysisof 犆犻犘犝犅 22transgenic 犃狉犪犫犻犱狅狆狊犻狊 lines

! １９６６ " # $ % & ３８ º Ｌ ＡＢＡ １５０ｍｍｏ Ｌ ＮＣ Ｌ ÔÕÖ±YÇ?È Ａ Ｄ Ç?ÈkÄ ³ÎÏØÒÙ+Ü Ｅ Ｈ»¼ １μｍｏ k ４００ｍｍｏ kä ³ÎÏ? yz X7? ( ６ 6}¼ X ６ pi±y ÎÏEÇ?ÈØÒÙåØ Ａ Ｄ Ｓ ｄｇ ｍ ｎ ｏｎ ｏ ｈｗ ｄ ｎ ｃ ｎ Ｅ Ｈ Ｐｈｎｏ ｏ ｈｗ ｄ ｙｐｎｄ犆犻犘犝犅２２ ｇｎ ｙｐ ｙｐｎｄ犆犻犘犝犅２２ Ｌ ＮＣ Ｌ ＡＢＡｎｄ４００ｍｍｏ Ｌ ｍｎｎ ｕｍｕｐｐ ｍｎ ｄｗ ｈｏ ｗ ｈｏｕ １５０ｍｍｏ １μｍｏ ｏ ｎ ｃ ｎ ｏｎ１ ２ＭＳｍｄ ｇｎ Ｐｈｏ ｏｇ ｐｈｗ ｋｎ６ｄｙ ｈｎｄｏ ｃ ｏｎ Ｆ ６ Ｇ ｍ ｎ ｏｎ ｙ ｏ ｈ ｎｇｎ ｃ ｎ ｎｄｗ ｄ ｄ ｎ ｍｎ ｇ ｙｐｕｎｄ ３ ~ ² Ý 犆犻犘犝犅２２ ô E # $ _ W õ c @ & j 犘犝犅２２ p )X Y y t v w RJ${ ªk ] E ` a y t k v Ûb Z ` î #$ ² 1ì ＡＢＡ ±Y Á 2 １２ \ ] Ò Ó 犆犻犘犝犅２２ 9 ＡＢＡ ±Y １ｈ ² ³ª > i ±Y { ７ ½ o Ï?YúaÄ g a» ã ä D Ä x p ３ｈ ² ³ª Þ Ei±Y{ ³ª ì ²?$Ëmõ{ ú Ð Þ û Æ ' è é { Ä? $ ６ｈ ²Û Ü i ± Y { ³ ª Ý 犆犻犘犝犅２２ kà?$ Á 2 ã ä D Ä Ò ù ô : ôn ＡＢＡ Á 2 t ì «E@ & j pì ² s Ｅ３ ãäåæç 9 ú Ð Þ û / è é n Ò Z A J õrüÿì)=> e x» Ｅ３ ãäåæç{=>ab2 ß [ ＡＢＡ 9#$? Ò ^ k ` a W X Ä n Aõ\]< É Ê Ë 犘犝犅２２ k 犘犝犅２３ & Ò Z A J e # à G Ò ^ E % à G Ø ¹ Ò ２１ A õ = > < h N Ｕ ｂｏｘ È Ｅ３ ã ä å æ À?$ Á 2 r x» ＡＢＡ p ; １２ 9 \ ç]eàgøòä Y ＡＰＵＢ１８ k ＡＰＵＢ１９ x ]n HÄ Ｂ FÑÒÓ ２ i c Ð { è é 犃狋犘犝犅１８ K 犃狋犘犝犅１９ È Ｅ３ ãäå æ ç 犘犝犅２２ ì ic Ｕ ｂｏｘ È Ｅ３ ã ä å æ ç YÜ ｐ ｎ Ｕ ｂｏｘ 犆犻犘犝犅２２ ytkvb {pi Û{Á2E&j{«ì $ ² E Ç? È a \» ＡＢＡ k v % { à G Ø Ò x õ $ D r x

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