V7109 07/2001
K-7000.. 4 K-7000... 5 K-7000... 5 5 5 6 7 7 7 7 K-7000... 8 K-7000.. 8 K-7000.. 8 K-7000.. 9 9 9 10 10 10 11 11 11 13 13 13 13 13 14 14 15 15 15 15 400 mosmol/kg NaCl... 17 17 18 M< 500 g/mol 18 M> 500 g/mol 20 20 20 EuroOsmo 7000 Software K-7000 22 EuroOsmo 7000 22 CD ROM EuroOsmo 7000 22 EuroOsmo 7000 software 22 22 23 26 26 27 <500. 27 >500. 28 29 31 32 K 7000.. 33 34 KNAUER... 35 37
4 K-7000 K-7000 ± 10 3 C 1 T 20 130 6 1-4 2
5 K-7000 K-7000 K-7000 1 K-7000 85~264V 47~440Hz 3
6 K-7000 3 1 Electrical Connection Syringe Ports Cell Cover Observation Window 1 Monitor Window Syringe Ports Position Red Position Yellow Position 1-4 4 4 Electrical Connector Syringe Ports Cell Cover Teflon Seal Syringe Guides Teflon Support Thermistors Support Rods Glass Beaker
7 K-7000 K-7000 Head Thermostat Monitor Window Cell Cover Front Panel Mirror Thermistors Glass Beaker 2 A0429 6 20ml 1#
8 K-7000 6 K-7000 K-7000 / Knauer Osmometer 130 TEST 15 Test OK maintenance required( ) 3 K-7000 7 2 Display Start/Stop Taste Autozero Taste Autotest Taste Pfeiltasten /
9 K-7000 2 START/STOP / AUTOZERO TEST 2 20-30 Right Arrow Key Up Arrow Key Down Arrow Key K-7000 8 3 K-7000 Singal/Measurement Value / Page Selection Time Selectable Gain 1 Settings Selectable t t +/-4 30 0.1 1,2,4,8,16,32,64,128,256 6 1 2 t t T head T cell 35
10 K-7000 1. 2. 60 62 602 5 / / / 9 10 10 50% Osmol/Kg
11 K-7000 400Osmo/Kg NaCl 16 455 455 400 calibrating( ) 10 400. 11 100% 100% 100% Reset Bridge Voltage? Confirm by cursor.( ) 100% 1. ; 2. ; 3. 4. 5. 6. 7. 15
12 K-7000 4 mol/kg 56.2 37-45 0.005 81.6 37-60 0.001 80.2 25-60 0.001 156.2 60-130 0.005 149.5 37-90 0.003 126.1 45-90 0.005 46.3 25-37 0.001 76.6 25-45 0.001 131.7 60-90 0.005 61.2 25-45 0.003 80.8 37-60 0.005 Dichlorbenzene 179.5 90-130 0.005 34.6 * 25-30 0.005 N,N-Dimethyl Formamide 155 90-120 0.005 DMSO 100 60-90 0.005 101 60 0.005 78.3 45-60 0.002 77.1 37-60 0.002 38.3 25 0.005 131.6 60-90 0.005 83.6 25-60 0.003 100.5 37-60 0.005 Furane 32 25 0.001 98.3 37-60 0.005 68.8 37-60 0.005 164.6 90-130 0.005 64.7 45 0.005 96.5 37-60 0.005 79.6 37-60 0.005 Methyl-iso-Butyl Ketone 116.9 45-60 0.005 Methylen Chloride 40.6 * 25 0.003 n-propanol 97.2 45-60 0.002 115.5 60-90 0.001 82.6 45-60 0.010 THF 65.5 45 0.005 110.8 37-90 0.001 87 37-60 0.001 100 37-60 0.005 o-xylene 143 90 0.001 *) (d)
13 K-7000 12 A0433 4 3~5 1. 2. 5 3. AUTOZERO 14
14 1. 13 2. 13 3. 13 13 mv 13 2
15 1. 2. 0.9mm 6 4 5 5 Temperaure Stabilization Time min K-7000 K-7000. Kcalib kg/mosmol
16 15 Measurement Value MV mv Slope mosmol mosmol C in kg kg Table 6 Relation between osmolality, freezing point depression and molality gnacl R mosmol KgH 2O mmol T Kg Kg 100 0.1858 3.09 104.2 1.04 200 0.3716 6.24 213.6 1.07 300 0.5574 9.45 323.4 1.08 400 0.7432 12.69 434.3 1.09 500 0.9290 15.94 545.6 1.09 600 1.1148 19.18 656.4 1.09 700 1.3006 22.45 768.3 1.10 750 1.395 24.10 824.8 1.10 1200 2.23 38.76 1326.4 1.11 1800 3.35 58.01 1985.3 1.10 2500 4.65 79.97 2736.8 1.09 3000 5.58 95.40 3264.9 1.09 *)R (osmolality) molality P8 K-7000 P9 Cell Temperature TC: 60 60 Head Temperature TH: 2 2 Measurement Time: 1.5 min 1.5 Gain: 16 16 ( 15 5).
17 400mOsmol/kg NaCI 1. 400mOsmol/kg NaCI 2. 1 3. 2 4. 5. 3 4 6. 5 7. Autozero 20-30 0 8. 3-5 9. START OVL 1.5 HOLD 3 0.5% Table 7 mosmol/kg 1 400 43.37 2 400 43.51 3 400 43.47 4 400 43.61 5 400 43.54 0.09% Kcalib= 0.1088 kgmosmol
18 3-5 3 8 1 34.43 2 34.61 3 34.50 34.51 0.03% *), 7 M<500g/mol n = m = M = 500g/mol mol/kg / kg/mol: K calib =MV/c C g/kg Kg/g K calib
19 16 ( ) 9 10 9 ( ) mol/kg 1 0.042 22.35 2 0.042 22.19 3 0.042 22.24 22.26 0.04% K calib 530kg/mol 10 g/kg 1 18.45 31.41 2 18.45 31.52 3 18.45 31.45 32.46 0.02% K meas 1.71kg/g M 530/1.71 309.9g/mo
20 M>500g/mol >500g/mol 1. 2. 3-5 3. 4. C mol/kg 5. C=0 K calib 17 M 500g/mol 6. 3-5 7. 8. C mol/kg 9. C=0 K calib
21 18 >500g/mol 11 PS10100 C mol/kg mv MV/C Kg/mol 1 0.001 0.71 710 2 0.002 1.28 640 3 0.003 1.78 593 C=0 Kcalib 527.6kg/mol 12 C g/kg mv MV/C Kg/g 1 11.50 0.71 0.0617 2 20.12 1.25 0.0621 3 28.79 2.07 0.0719 C=0 Kmeas 0.054kg/g M 527.6/0.054 9770g/mol
22 EuroOsmo 7000 Software K-7000 EuroOsmo 7000 Software K-7000 K-7000EuroOsmo 7000 1.2 35 K-7000 and the 7000 EuroOsmo EuroOsmo Pentium 130 MHz 32 MB 16 MB Windows NT 4.0 Windows 95 O/S Service Pack 3 (or later) Service Pack 1 20 MB CD ROM EuroOsmo 7000 1. 2. 30 3..... EuroOsmo Setup.exe 7000/Disk 4.. EuroOsmo 7000 1. 2. 3. A 4. Setup.exe 5.. K-7000
23 EuroOsmo 7000 Software K-7000 / /Eurosomo 19 19 EuroOsmo7000 13 File Edit View Run Help File Edit View Run Help EuroOsmo7000 Windows Osmo MW<500 500g/mol MW>500 500g/mol
24 EuroOsmo 7000 Software K-7000 20 14 START T SCROLL/ / / <AND> 4 1. 2. 3. 4.
25 EuroOsmo 7000 Software K-7000 20 4 21 < > 24 20 <500g/mol (B), A 2 C M 22. Setup 23
26 EuroOsmo 7000 Software K-7000 23 K-7000 EuroOsmo 20~130 0-6 t t t Apply Calibration Sample List AutoZero AUTOZERO
27 EuroOsmo 7000 Software K-7000 Osmol Start Calibration t osmogram 5 4/1.1 Kcalib 3 Measure View Sample List 1 NaCI Start 20 # <500g/mol MW<500 24 <500 mol/kg 24
28 EuroOsmo 7000 Software K-7000 25 3 25 26 <500 # >500g/mol MW>500 osmogram 20 17
29 EuroOsmo 7000 Software K-7000 27 2 Kcalib c=0 M/V 21 18 28 5 20 Kcalib Kmeas EuroOsmo 1. 2. 3. 4..osm Windows
30 EuroOsmo 7000 Software K-7000 EuroOsmo.cls.cll <500.clg >500 29
31 5 0.9cm 8
32 15 A3701 A3703 A0807 A0429 A0433 Y1277 Y1026 V7109 20-130 10 / 7 10g l 110 V or 220 V 16 A0428 A0430 12 Y1241 10 NaCl 400mOsmol/kg A3702 EuroOsmo 7000 Software Package for Windows 95 / 98 / NT, (RS232 cable ncluded)
33 EuroOsmo 7000 K 7000 EuroOsmo7000 10,000g/mol 40-40,000g/mol 0.001-15molal -5 mol/kg 3.3x10 1.7x10-4 mol/kg 1x10 20-130 T 6 400.5h 60 1.0h 100 1.5h 130 2.0h 1ml 20ml 1-256 85-264V/47-440Hz,60VA RS232PC 1V 160x182x340mm 5.4kg