2546 Jounal of Chinese Medicinal Mateials 40 11 2017 11 UPLC-DAD 28 * 310052 - UPLC-DAD 28 70% Agilent Eclipse Plus C 18 100 mm 2. 1 mm 1. 8 μm -0. 05 mol /L DAD 28 0. 001~ 0. 008 mg /g 88. 2% ~ 101. 5% RSD 3. 9% 28 R282. 5 A 1001-4454 2017 11-2546-05 DOI 10. 13863 /j. issn1001-4454. 2017. 11. 012 104656 B 40816 1-4 41028 G 20124 O D. Ehenstofe GmbH 1- V209G005 G 1128G005 S Z315G005 2G 9A59G005 CNW Technologies GmbH 21 50970050 22 O 41670085 2 35620050 73 O 860322 1 ~ 3 LL80P62 UPLC-DAD 21 7 20 10 1 1 1. 1 LC-20AD Coydalis yanhusuo W. T. Wang SHIMADZU XPE205 Cathamus tinctoius L. Mettle Toledo Milli-Q Millipoe Elmasonic P 2 Elma 5424 Eppendof 2. 1 Agilent Eclipse Plus C 18 100 mm 2. 1 mm 1. 8 μm A 0. 05 1. 2 114377 2 41109 73 99860 2G 91792 28 30711 110659 40903 210 ~ 700 nm 2 μl 92153 31113 10 000 50602 Ⅰ 41126 Ⅱ 112208 Ⅲ 50306 Ⅳ 31224 7B 41112 G mol /L B 0 ~ 20 min 5% ~ 70% B 20 ~ 25 min 70% ~ 95% B 25 ~ 32 min 95%B 0. 25 ml /min 35 DAD 2. 2 2. 2. 1 1 21 22 2 73 2017-05-10 1981- Tel 0571-87180345 E-mail luolei33988@ 163. com * Tel 0571-86459425 E-mail mk033@ excite. com
Jounal of Chinese Medicinal Mateials 40 11 2017 11 2547 1 20 10 1 ml 10 ml 70% 1 16 21 2 2 17 2 O G S 3 18 2G 70% 4 19 0. 03724 mg /ml 2 5 20 0. 04536 mg /ml 0. 05216 mg /ml O 6 21 0. 01940 mg /ml G 0. 04716 mg /ml S 7 22 0. 04420 mg /ml 2G 0. 05556 8 23 mg /ml 7 1 9 24 10 25 ml 10 ml 70% 7 11 26 12 27 2. 2. 2 2 g 70% 13 28 20 ml 250 W 40 khz 20 min 14 29 0. 22 μm 15 30 2G 28 2. 3 21 22 Ⅰ Ⅱ Ⅲ 2 73 2G 28 Ⅳ 7B G 1- Ⅰ B G 70% Ⅱ Ⅲ Ⅳ 7B G 0. 04416 mg /ml 21 0. 04084 mg / ml 22 0. 04228 mg /ml 0. 04584 mg / ml 2 0. 04220 mg /ml 73 0. 04188 mg /ml 2G 0. 04332 mg /ml 28 O G S 0. 04484 mg /ml 0. 04152 mg /ml 2G 7 0. 04096 mg /ml 0. 04612 mg /ml 430 nm 7 0. 03964 mg /ml 0. 02480 mg /ml 430 nm Ⅰ 0. 02148 mg /ml Ⅱ 0. 03212 mg /ml 2. 4 2. 2. 1 /1 Ⅲ 0. 02232 mg /ml Ⅳ 0. 01716mg /ml 21 2 μl 2. 2. 1 /2 7B 0. 01844 mg /ml G 0. 02244 mg /ml 1-0. 04508 mg /ml B 0. 05340 mg /ml G 21 1- B G 21 500 nm 21 500 nm 2 7 2 μl 2. 1 21 1 500 nm 21 HPLC 1. 2. 3. G 4. 5. 2G 6. 7. 8. 73 9. 28 10. 2 11. 12. 21 13. B 14. 22 15. 1-16. G 17. Ⅰ 18. Ⅱ 19. Ⅲ 20. 7B 21. Ⅳ
2548 Jounal of Chinese Medicinal Mateials 40 11 2017 11 2 430 nm 7 HPLC 1. 2. S 3. 2G 4. 5. O 6. 2 7. G 500 nm 7 430 μl 2 4 8 12 16 24 h nm 1 2 28 RSD 1. 5 2. 5 1 2. 2. 1 / 2. 8 1 21 1 2 5 μl 21 6 1 2 5 μl 2. 2. 2 2. 1 500 nm 1 2 2. 9 LOD 3 1 2 2 2. 2. 1 /2 7 1 2 5 μl 7 0. 008 mg /g 1 2 5 μl 2. 1 430 nm 2. 10 3 2. 6 21 2. 11 20 7 2 μl 2. 1 10 2. 2. 2 2. 1 6 28 2 O RSD 2. 0% 28 O 2. 0% 24 h 2 3 28 LOD 3 O 2. 7 2 4 O 200 ~ 700nm 3 430 nm O a HPLC
Jounal of Chinese Medicinal Mateials 40 11 2017 11 2549 4 430 nm O HPLC 5 O O 6 DAD 5 2 O DAD 6 O DAD 21 500 nm /ng / mg /g 1 21 4. 42~ 220. 8 Y = 10937X-7909 1. 0000 0. 002 96. 2±1. 3 2 22 4. 08~ 204. 2 Y = 21899X-9374 1. 0000 0. 001 96. 6±1. 3 3 4. 23~ 211. 4 Y = 7777X+173 1. 0000 0. 002 98. 5±1. 2 4 2 4. 58~ 229. 2 Y = 10858X-2835 1. 0000 0. 002 96. 1±1. 3 5 73 4. 22~ 211. 0 Y = 8904X-1343 1. 0000 0. 002 97. 4±1. 3 6 2G 4. 19~ 209. 4 Y = 5482X-665 1. 0000 0. 003 101. 1±1. 6 7 28 4. 33~ 216. 6 Y = 10409X-5487 1. 0000 0. 002 95. 4±1. 3 8 4. 48~ 224. 2 Y = 6543X-5701 1. 0000 0. 002 98. 7±1. 2 9 4. 15~ 207. 6 Y = 5673X-935 1. 0000 0. 004 97. 6±1. 3 10 4. 10~ 204. 8 Y = 8920X-1100 1. 0000 0. 002 100. 0±3. 0 11 4. 61~ 230. 6 Y = 8874X-454 1. 0000 0. 002 101. 5±1. 9 12 3. 96~ 198. 2 Y = 7809X-1407 1. 0000 0. 002 99. 7±1. 8 13 Ⅰ 2. 48~ 124. 0 Y = 11310X-2125 1. 0000 0. 003 96. 9±1. 3 14 Ⅱ 2. 15~ 107. 4 Y = 10757X-2329 1. 0000 0. 003 96. 5±1. 3 15 Ⅲ 3. 21~ 160. 6 Y = 13222X-28158 0. 9990 0. 003 90. 5±2. 0 16 Ⅳ 2. 23~ 111. 6 Y = 11004X-14984 0. 9991 0. 005 91. 2±1. 5 17 7B 1. 72~ 85. 8 Y = 10540X-5793 0. 9997 0. 004 92. 1±2. 3 18 G 1. 84~ 92. 2 Y = 12851X-1171 1. 0000 0. 003 96. 6±1. 3
2550 Jounal of Chinese Medicinal Mateials 40 11 2017 11 2 /ng / mg /g 19 1-2. 24~ 112. 2 Y = 4925X-860 1. 0000 0. 008 96. 5±1. 3 20 B 4. 51~ 225. 4 Y = 7837X-432 1. 0000 0. 003 97. 6±1. 2 21 G 5. 34~ 267. 0 Y = 7106X-757 1. 0000 0. 002 99. 4±1. 4 3 7 430 nm /ng mg /g 1 2 3. 72~ 186. 2 Y = 12860X-2533 0. 9991 0. 005 88. 2±3. 9 2 4. 54~ 226. 8 Y = 13200X+233 1. 0000 0. 002 98. 0±1. 0 3 O 5. 22~ 260. 8 Y = 23834X-30308 0. 9999 0. 002 96. 4±0. 8 4 G 1. 94~ 97. 0 Y = 9914X-1712 1. 0000 0. 006 93. 6±1. 0 5 S 4. 72~ 235. 8 Y = 12343X-3195 1. 0000 0. 003 92. 1±0. 8 6 2G 4. 42~ 221. 0 Y = 7187X+219 1. 0000 0. 004 90. 2±0. 6 7 5. 56~ 277. 8 Y = 16445X-22314 0. 9998 0. 002 98. 8±0. 9 3 UPLC Agilent E- clipse Plus C 18 100 mm 2. 1 mm 1. 8 μm 0. 25 ml /min HPLC 1. J. 2 2011 30 9 698-699. 2. J. 2014 20 18 7 95-98. 3. HPLC-DAD - 70% 20 J. 2015 34 5 655-657. 21 500 nm 4. HPLC-PAD 500 nm J. 7 430 nm 2010 20 11 2724-2725. 430 nm