33 3 OOD & MACHINERY 217 3 食品与机械 Vol.33No.3 Mar.217 DOI:1.13652/j.issn.13-5788.217.3.29 Extractionprocessoptimizationandanticanceractivityofmaizeyelowpigment YU Hui LIU Mei-jia LIU Jian-li CAO Xiang-yu ( 1136) (Schoolof LifeScienceLiaoning UniversityShenyangLiaoning1136China) ultrasonicpower4 Wenzymolysistemperature35 themaxi- : celwerestudiedbycelmorphologicalobservationand MTTtest. (BGC-823) Conclusion:Theextractionofmaizeyelowpigmentbyultrasonicas- sisted with enzymatic method is a kind of efective extraction ; BGC-823 methodthe maizeyelow pigmentpreparedhasstronginhibitory efectonbgc-823cels. MTT Keywords:maizeyelowpigment;ultrasonic;enzymicmethod;anti- : cancer 1U/g 4 W 35 (72.64±.42) μ g/g; (Zea maysl.) MTT BGC-823 (28~22) BGC-823 53% [1] E : ; ; Abstract:Objective:Tostudythebestpreparationtechnologyof maizeyelowpigmentbyultrasonicassistedwithenzymaticmethod [2] andtheinhibitoryefectofthem onthegrowthofhumangastric cancercels(bgc-823).methods:theoptimumconditionsofmaize yelowpigmentwereextractedbyultrasonicassistedwithenzymatic methodbysinglefactorandresponsesurfaceexperiments.theinhi- bitionof maizeyelow pigmenton BGC-823cels wasdetectedby morphologicalobservationand MTTassaytoevaluatetheantitumor activity.results:themaizeyelowpigmentbestextractioncondition ofultrasonicwithenzymaticmethodis:enzymeactivity1u/g mumextractionyieldwas(72.64 ±.42) μ g/gunderthecondition themaizeyelowpigmenthasacertaininhibitoryefectonbgc-823 [1] [9] : ( : [11] BGC-823 31245); ( :21513); ( :LJQ2132); ( :L2147); : : (198 ) E-mail:xycaolnu@163.com :216 12 15 (CornColor) [3] β- [4] [5] [6] [7] [8] [9] [6] 139
217 3 (1) : 2.g 48 [12] 12162 U/g 4 ml 4h 3 6 ml (BGC-823) 2min 2 W 4r/min 5 min 446nm 3 (2) : 2.g 1 1.1 : 11 ; 6 ml 2 min 2 W :BGC-823 ; 4r/min 5min 446nm β- : 98% 3 ; (3) : 2.g : 2U/g 12U/g 4 ml ; 4h 4 6mL 3-(45- -2)-25- (MTT): 2 min :1234 98% Sigma 1.2 3 :BS223S 1.3.4 Box-Behnken Design-Expert8..6 ; Box-Behnken :JW-321HR ( ) ; ) ; 1.3.5 :721N (1) : ; [14] :823 4 1 5 6 :CHRIST ; 2mL 37 5%CO2 :RE-52CS-2 24h ( ; :Sunrise 1.3 (2) : 1.3.1 [15] :8233 1 3 96 [1] 12 2μL 24h (.25.51.mg/mL) 24hMTT 4r/min 5min 1.3.6 3 SPSS 19. : 1.3.2 β- [13] : β- 5.mg 12U/g 4 ml 4h :23456 5 W 4r/min 5 min 446nm 3 3 :OLYMPUS DP73 (.25.51.mg/mL) 24h 2 2.1 28~6nm β- 446nm β- 5mL 1.2.3.4.5.6.mL 446nm 1mL 1 446nm β - 446nm β - (R 2 =.9996) 1 2.2 1.3.3 2.2.1 2 3 : 14
33 3 : 1 β- Figure1 Standardcurveofβ-carotene 4 Figure4 Efectofultrasonicpowerontheyieldof maizeyelowpigment 4 W (65.99±.48) μ g/g 2.3 Box-Behnken 1 1 Box-Behnken 2 Table1 FactorsandlevelsinBox-Behnkendesign Figure2 Efectofenzymedosageontheyieldofmaize yelowpigment A / B / C / (U g -1 ) W 12U/g -1 1 12 35 4 35 4 1 14 45 45 12U/g 2 [16] 12U/g 2 Box-Behnken (64.78±1.285) μ g/g Table2 Box-Behnkendesignwithexperimentalresults 2.2.2 3 A B C /( μ g g -1 ) 4 1 1-1 58.8 (65.69±1.21) μ g/g 4 2 4 3 68.31 67.96 2.2.3 4 4-1 -1 72.64 5 1 4 W 6 1 59.7 68.13 7 1 1 6.92 8-1 1 62.35 9-1 1 7.84 1 68.3 11-1 -1 66.32 12 1-1 67.23 13 67.91 14 1 1 65.72 3 15 1-1 63.91 Figure3 Efectofenzymesolutiontemperatureonthe yieldofmaizeyelowpigment 16 17-1 -1-1 1 65.81 71.14 141
217 3 2.4 Design-Expert8..6 Table3 Analysisofvarianceforthefitedregressionmodel F Pr>F : 251.17 9 27.91 131.42 <.1 ** Y=68.-4.7A+1.28B-1.18C-.91AB-.37AC+ A 176.81 1 176.81 6534.66 <.1 **.49BC-1.14A 2-3.1B 2 +.22C 2 (1) B 13.3 1 13.3 481.58 <.1 ** 3 P<.1 P= C 11.19 1 11.19 413.43 <.1 ** AB 3.28 1 3.28 121.8.4223 <.1 ** AC.56 1.56 2.51.27 ** BC.95 1.95 35.13.6 ** A 2 5.46 1 5.46 21.88 <.1 ** (P<.1); B 2 38.25 1 38.25 1413.61 <.1 ** (P<.5) F C 2.21 1.21 7.77.27 * A>B>C R 2 =.19 7.27.9992.89 3.3 1.18.4223.1 4.25 2.5 251.36 16 5(a) ** (P<.1);* (P<.5) 3 5 Figure5 Responsesurfaceandcontourplotsfortheefectsofinteractionbetweenenzymeactivityandultrasonicpowerbe- tweenenzymeactivityandenzymesolutiontemperaturebetweenultrasonicpowerandenzymesolutiontemperature ontheyieldofextractionratio 142
33 3 :.1) [17] BGC- ; 1U/g 4 W 5(b) ; ** P<.1 5(c) 7 823 ; Figure7 Inhibitoryefectofmaizeyelowpigment on823cels ; 4 W 35 2.8 8 823 2.6 1. mg/ml Design-Expert8..6 22.1% 1U/g 413.99 W [9] 35 53.21μg/mL (ES-2) 72.9μg/g 5% 62.2161.9398.6μg/mL : (MDA-MB- 1U/g 4 W 35 3 231) (A549) (PC-3) (72.64±.42) μ g/g [18] (Hela) 2μg/mL 31.98% 2.7 6 7 1.mg/mL 47.2% (P< 823 ** P<.1;* P<.5 8 823 Figure8 Efectofmaizeyelowpigmentonviability of823cels 3 3 : 1U/g 4 W 35 ; (72.64±.42) μ g/g 6 823 MTT Figure6 Efectofmaizeyelowpigmenton823cels 143
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