better than big brands and save... Western Blot 1 Sample Preparation Total Protein Extraction Kits, 3 ; denatured 1 (2-8 mg/ml), denatured native prot

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1 Western Blot 3 OK! 1 Sample Preparation Blocking / Hybridization ANTEC Bioscience Inc 台北 新竹 台中 台南 高雄 A040831

2 better than big brands and save... Western Blot 1 Sample Preparation Total Protein Extraction Kits, 3 ; denatured 1 (2-8 mg/ml), denatured native proteins Detergent-free buffer (P505/P506) 101Biototal protein kits RIPA buffer Procedure time 1 ~ 3 minutes 45 minutes No protein lost Cat. No. Description Sample type Protein Status Time Size P501 Total protein kit cells Denatured/Native 1 ~ 3 min. 20 Rxns P502 Total protein kit tissues Denatured/Native 1 ~ 3 min. 20 Rxns P505 Detergent-free kit cells Denatured/Native 5 ~ 8 min. 20 Rxns P506 Detergent-free kit Tissues Denatured/Native 5 ~ 8 min. 20 Rxns 2

3 Cytoplasmic & Nuclear Extraction Kit, 10 ( mg/ml) SDS-PAGE ELISA IP Protein Localization Gel Mobility Shift Assays (GMSA) Cat. No. Description Sample type Protein Status Time Size P504 Nuclear protein kit cells / tissues Native 10 min. 20 Rxns Plasma Membrane Protein Extraction Kit, 45 protein-protein interaction Buffer detergent EDTA, (1-50 millions / sample) 101Bio Qiagen Thermo Abcom Detergent-free Yield High Low Low Low Keep protein-protein interaction Disrupt Disrupt? Easy to handle Cat. No. Description Sample type Protein Status Time Size P503 Membrane protein kit cells / tissues Native & Detergent-free 20 ~ 45 min. 20 Rxns 3

4 better than big brands and save... Western Blot 1 Sample Preparation Mitochondria Isolation Kit, (5-40 millions / sample) detergent EDTA, ~30 Cat. No. Description Sample type Protein Status Time Size P507L Mitochondria kit cells / tissues Native & Detergent-free 25 ~ 30 min. 50 Rxns Histone/DNA Binding Protein Extraction Kit, ~10 (1~2.5 mg/ml) SDS-PAGE immunoblottings ELISA IP Protein Localization modification studies High efficient extraction of both histon and other DNA binding protein. Cat. No. Description Sample type Protein Status Time Size P Histone/DNA binding protein kit Cells Denatured < 10 min. 25 Rxns 4

5 Protein Extraction Kit from Gel Slices,10, Cat. No. Description Sample type Time Size P Protein gel slice kit Gel slices < 10 min. 20 Rxns 5

6 Western Blot 1 Sample Preparation Protease Inhibitor,,, Item Number Item Description Qty/Pk Protease Inhibitor Cocktail Set III, EDTA-Free 1ml / 1 set ( 5 x 1ml ) Phosphatase Inhibitor Cocktail Set V, 50X 1ml / 1 set ( 5 x 1ml ) MG mg / 5mg / 10mg / 20mg Protease Inhibitor Cocktail Set I 1ml / 10ml ( 10 x 10ml ) JNK Inhibitor II 5mg / 25mg / 50mg PD mg / 10mg / 25mg SB mg / 5mg / 10mg Autophagy Inhibitor, 3-MA 20mg / 100mg U0126 1mg / 5mg / 10mg / 20mg Y mg / 10mg / 100mg Western Blot 2 ProSieve TM EX Running buffer : 10X buffer : 18~35 separation : Tris-glycine or Laemmli system gel ProSieve TM EX running buffer Min OK 15 minutes run with ProSieve TM EX buffer 40 minutes run with Tris-Glycine buffer 6

7 Western Blot 3 ProSieve TM EX Transfer buffer : 10X buffer : 10~15 transfer ProSieve TM EX transfer buffer : PVDF, NC or Nylon membrane Min OK 10 minutes transfer with ProSieve TM EX buffer 60 minutes transfer with standard transfer buffer Western Blot 3 SE250 & SE260 : SE250 : 10 x 8 cm SE260 : 10 x 10.5 cm, 10 x 8 cm SE640 : 18 x 8 cm SE600 & SE660 : SE600 : 18 x 16 cm SE260 : 18 x 24 cm, 18 x 16 cm : : : 4 / TE22 : 4 9 x 10 cm gels TE62 : 4 15 x 21 cm gels 16 7 x 10 cm gels TE70X & TE77X TE70X : 14 x 16 cm 4 mini gels TE77X : 21 x 26 cm 12 mini gels PS200HC V, ma, 200 W PS300B V, ma, 90 W PS V, ma, 150 W 7

8 Western Blot SNAP i.d. 2.0 : blocking / incubation : 30, WB :, : 4 membrane : Overblocking,, :, (4.5 x 8.4 cm). ( 7.5 x 8.4 cm ) ( 8.5 x 13.5 cm ) Holder 4, membrane substrate 4 Blocking / Hybridization 3.5~4 SNAP i.d. 2.0 : Application Cat No. Description Qty/Pack SNAP2MINI SNAP i.d. 2.0 System-Mini (7.5 x 8.4 cm) 1 SNAP2MIDI SNAP i.d. 2.0 System-Midi (8.5 x 13.5 cm) 1 SNAP2MM SNAP i.d. 2.0 System-Mini and Midi (7.5 x 84 cm and 8.5 x 13.5 cm) 1 SNAP2MB1 SNAP i.d. 2.0 System-Mini and MultiBlot 1 WB SNAP2MB2 SNAP i.d. 2.0 System-Midi and MultiBlot 1 SNAP2BHMD0100 SNAP i.d. 2.0 Midi Blot Holders, 100/pk 100 SNAP2BHMN0100 SNAP i.d. 2.0 Mini Blot Holders, 100/pk 100 SNAP2BHMB050 SNAP i.d. 2.0 MultiBlot Holders (50 pack) 50 SNAP2BHMB-K NAP i.d. 2.0 MultiBlot Holders-Bulk (200 pack) 200 8

9 Bløk Noise Cancelling Reagents Blocking Western blotting,, /, : 1., 2., Bløk, WB CH,FL,PO, :Coomassie Blue,, 2, ReBlot TM Plus Mild/Strong Antibody Stripping Solution,, PVDF re-blot, ReBlot TM Plus Western Blot Recycling, 2,900 ReBlot TM Plus Western Blot ReBlot TM Plus - ReBlot TM Plus NC PVDF ReBlot TM Plus, β-,, 15min,, ( Edman ) 9

10 Western Blot 4 Blocking / Hybridization SignalBoost TM Immunoreactions Enhancer Kit, SignalBoost TM, Western blot ELISA 10% Western Blot 4 Blocking / Hybridization Affinity Purification 20 ul binding mg IgG / ml,, 100 rxns Antibody-binding protein specificity for Protein A, Protein G, and Protein A/ G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Cat. No. Description Binding Capacity Size PAG SEPHAROSE PROTEIN A/G mg IgG 2 ml PA SEPHAROSE PROTEIN A 35 mg IgG 2 ml PG SEPHAROSE PROTEIN G 10 mg IgG 2 ml 10

11 Western Blot FluorChem 5 Detection FluorChem R FlourChem M FluorChem E FluorChem Q FluorChem HD2 IR Infrared Fluorescence 3-Color Fluorescence Chemiluminescent Blots Protein Gels DNA Gels X-ray Film Colony Counting Colorimetric Detection Light Sources IR Fluorescent LED Epi-Excitation RGB Fluorescent Epi-excitation 365/302 nm UV Transilluminator Epi White Lights Trans White Lights Filter Positions 10 - motorized 10 - motorized 6 - motorized 6 - motorized 5 - motorized Emission Filters 5 included : 835/70 nm 710/40 nm 607/36 nm 593/40 nm 537/26 nm 4 included : 710/40 nm 607/36 nm 593/40 nm 537/26 nm 1 included : 590/50 nm 620/40 nm (opt) 537/35 nm (opt) 460/40 nm (opt) 4 included: 537/35 nm 606/62 nm 699/62 nm 595/55 nm 1 included : 595/55 nm 完整的應用滿足多種影像擷取定量需求 Infrared Fluorescence UV Fluorescent DNA and Protein Gels Multiplex Westerns Chemiluminescent Westerns Chemiluminescent Macroarrays Colony Plates 2D Gels Animal Imaging Colorimetric Protein Gels 11

12 Western Blot 5 Detection Infrared Imaging Systems Western blot,, 6, JBC, 6 Odyssey Western blot,,,, substrate 6,000,Data! (Near-Infrared detection),, HRP substrate IR Fluorescent Direct Detection,,, substrate,, Accurate Quantification,,, ON-SCREEN Display Antigen on membrane 1 Ab binds to antigen IRDye labeled 2 Ab binds to 1 Ab 12

13 2013 LI-COR Frost & Sullivan NIR,,,, stripping re-probing! blot target, rabbit anti-erk1 primary Ab mouse anti-phospho-erk primary Ab, IRDye, goat anti-rabbit IRDye 680 ( ) secondary Ab goat anti-mouse IRDye 800CW ( ) secondary Ab, blot targets, targets ( ERK ( ) phospho-erk ( )), phospho-erk1 phospho-egfr Odyssey C-DiGit Quantitative Infrared Western Blots Multiplex Western Assays Chemiluminescent Western Blots Coomassie-Stained Protein Gels Nucleic Acid Gel Imaging SYTO 60 ODYSSEY CLx ODYSSEY Fc C-DiGit Infrared Imaging System Imaging System Imaging System Ethidium Bromide SYBR Family In-Cell Western Assays On-Cell Western Assays ELISAs Protein Arrays EMSA/Gel Shift Assays Small Animal Imaging Tissue Section Imaging Whole Organ Imaging 13

14 NanoPhotometer NP80,,, : 7 Quad Core 1 GHz 8 GB Linux, Windows Android ios vortex 0.3µl Sample Compression Technology TM Battery Powered Flexible Unit Control True Path Technology TM NP80 N60 N50 C40 (nm) dsdna : 1-16,500 ng/µl BSA : mg/ml dsdna : 1-16,500 ng/µl BSA : mg/ml dsdna : 5-7,500 ng/µl BSA : mg/ml 2 dsdna : ng/µl BSA : mg/ml - - dsdna : ng/µl BSA : mg/ml (mm) 0.07, 0.67, , , Vortex :C40 Implen Submicroliter Cell, + 2: :, : 14

15 ForteBio BLItz system 個人化生物分子交互作用分析系統 用範Buffer exchange 緩衝液置換, 如 : 層析前後緩衝液體系的改變 交互作用 (KD ) 新一代設計, 外型輕巧, 功能與 ForteBio Octet 系統相同的單通道系統 使用生物膜干涉技術 ( BLI ) 進行偵測, 儀器無任何流體管路, 樣品亦無需繁複的前處理工作 可使用多樣化生物感測探針 ( Biosensor ), 滿足不同的實驗需要 樣品直接測量, 無需任何標示, 且可直接使用未純化樣品 ( Crude Sample ) 即時數據呈現, 立即了解樣品結合特性 樣品體積需求最低只要 4µl 使用 USB 介面連接電腦, 安裝簡便, 使用容易 儀器尺寸 : 17.4 cm H x 15.3 cm W x 22.2 cm D 樣品只要 4µl!! 儀器重量 : 3.3 公斤 1 Drop. 2 Read. 3 Done. 滴上樣品 (4µl) 裝上感測探針, 測量 得到數據, 完成 量樣品 Labscale 實驗室小型切向流系統 Clarification 澄清, 如 : 分離細胞 / 細胞碎片 ( 細胞收集 / 細胞去除 ) Concentration 濃縮, 如 : 蛋白濃縮, 去除水 / 緩衝溶液 Desalting 脫鹽圍分子核酸 抗體應Frationation/purification 分離 / 純化, 如 : 蛋白 多肽 多醣 病毒 內病毒顆粒, Depyrogenation 除熱原, 如 : 中藥注射劑的熱原去除 極高的樣品回收率 零殘留樣品體積 最小起啟工作體積僅 15 ml, 系統管路殘留體積 <1.5 ml 可加接除菌過濾器, 避免細菌污染樣品 樣品處理量 樣品槽容積為 500 ml, 當需要處理更大體積樣品時, 可利用真空虹吸進行連續補液, 無需額外的幫浦作為動力 同一系統可以同時安裝 3 組膜包, 加大樣品處理能力 三種不同材質的濾膜適合不同的應用需求 第二代改良聚醚碸膜 - 提供更高流速及可耐更高壓力 ( KDa ) 第二代再生纖維素膜 - 提供更低的吸附量 ( KDa ) 聚偏二氟乙烯膜 - 優良的通透性及低吸附率, 可滿足菌體收集及粗產品的澄清 (0.1µm 0.65µm) 15

16 ANTEC Bioscience Inc 台北 新竹 台中 台南 高雄