?基因抗虫棉?境影响研究的?研?告

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1 Bt

2 Bt CK CpTI FAO IPM GK Bt 1

3 1 Bt Bt 4 Bt 5 6 Bt 7 Bt 8 Bt Bt

4 Bt (Bacillus thuringiensis Berliner) % Bt Bt Bt (Helicoverpa armigera H bner) Bt l Bt 2 Bt 3 4 Bt Bt Bt 5 Bt

5 1 (Bt) Bt 2000 Bt Bt 35 ( 1) 1 Bt Bt Bt % Est Est.7100 Est Monsanto Bt Bt 1997 Bt ( 2) (Bt) ( GK-2 GKl2) (Bt+CpTI 321) CpTI - Cowpea trypsin inhibitor ( ) Bt (%) Est

6 3 Bt Bt 10 ( ) Bt Bt 2000 Bt FAO IPM Bt 1996 Bt

7 Bt Bt (Helicoverpa armigera H bner) Bt 33B,GK-2, GK Bt ( 1) (Coccinella septempunctata) (Propylaea japonica) (Erigonidium graminicolum) (Chrysopa sp.) 6

8 (Orius mimutus) (Geocoris pallidipennis) Bt (Microplitis sp ) (Campoletis chlorideae Uchida) (R93-4 R93-4 ) A B ( 1999a) ( 2) Bt (Propylaea japonica) 2000 ( 2001) 4 A 7

9 2001 Bt Microplitis mediator Haliday Helicoverpa armigera (H bner) Bt Bt CK Bt Bt Bt 3 Bt 3 Bt 2001 (%) (%) (%) (%) 321 Bt + CpTI Bt Bt + CpTI Bt ( 33B) Bt Bt CK ( 12) Bt Harmonia axyridis (Pallas) Bt % B 2001 Bt (Campoletis chlorideae Uchida) 4 4 Bt % % Bt ( 33B) Bt Bt 33B 35B Bt GK2 GKl2 (Bt+CpTl) Bt 8

10 A (beet armyworm Spodoptera exajua) Bt 70 ( 3) B (Lygus bugs Adelphocoris saturalis A. fasciaticolls, Lygus lucorum etc ) Bt Bt GK12 33B 7 8 ( 4 5)( ) 9

11 GK12 Bt 321 Bt + CpTI Bt Bt C (Cotton spider mites) Bt D (tobacco whitefly Bemisia tabaci) 2000 Bt Bt GK12 33B Bt 3 Bt 7 18 ( 5)( 2001 ) Bt ( 1998) Bt Bt + 10

12 Bt A (Cotton aphids Aphis gossypii) Bt Bt Bt 1 ( 6) B (Cotton spider mite Tetranychus cinnabarinus) 6 7 Bt Bt Bt 8000 ( 7)

13 C (Lindemon onion trips Thrips tabaci) Bt ( ) Bt Bt 800 Bt Bt ( 8) D (Cotton whitefly Trialeurodes vaporariorum) Bt 37.2 Bt ( 1998) E (Lygus luconum) Bt 163 Bt F (Cotton leaf hopper Empoasca biguttula) Bt 55 Bt G Bt Bt Bt Bt Bt Bt ( 9) ( 1997) 12

14 9 Bt Bt GK19 Bt GK Bt Bt 2000 A (Cotton Aphids Aphis gossypii) Bt 1840 Bt 1140 ( 10) 2000 Bt MD Bt + Bt B (Carmine spider mite Tetranychus cinnabarinus) Bt 13

15 5 22 Bt ( 11) C (Cottonwhitefly Trialeurodes vaporariorum) Bt Bt Bt ( 12) D (Cotton Leafworm, Prodenia litura Fabticius) Bt 8 ( 13) 14 / 8 E (Black-striped plant bug, Adelphocoris saturalis Jakovlev) Bt ( 14) 14

16 15

17 F (Trrips tabaci Lindem) (Frankiniella intomsa Trybom) Bt % Bt 33B 33B 2 60% Bt 35% Bt ( ) Bt ( ) Bt ( ) Bt ( 2000a 2000b) 15 16

18 (Diversity Index) H = - PLnP (Everness Index) J = H/LnS (Dominant Concentration Index) C = p i Pi = ni/n s i=1 i i s i=1 2 ni N S 2 Bt (MD-80) ( 6) Bt Bt 1999 Bt ( 7) 7 Bt 1999 Bt B Monsanto 30 33B %~16.3% 71.0%~78.3%

19 8 Bt 1998 Bt Bt 33B

20 Bt 1 Bt 2 (Helicoverpa armigera) ( 1 ) Bt Bt GK ( 16) 50 ( 2000 ) 16 Bt 43.3 Bt 19

21 2000a Bt Bt Bt Bt 16 3 Bt Bt >Bt > Bt Bt Bt Bt 7 3 Bt Bt 2000b Bt CIylAc Bt Bt 6 9 Bt 8-10 ( 1998) Bt Bt 41 IC 50 50% µg/ml µg/ml, µg/ml µg/ml µg/ml µg/ml (IC99) 3 0% %, Cry1Ac Wu et al, Bt Cry1Ac F 19 LC g.L -1 F 2 LC 50 (0.2972g.L -1 ) Bt Cry1Ac LC g.L g.L -1 LC Bt 4 6.7% Bt % % 3.8% 1.6% Bt Bt Bt 33B % % 75% 4 57% 78% 1995 Bt ( 1998) 5 Bt Bt 17 Bt

22 ( ) Bt Bt Bt Bt 33B CK CK CK CK Bt Bt Bt RR 1 Bt ELISA 3 Bt 33B GK12 GK2 > > > Bt 2001 Bt Bt Bt Bt ( ) Bt Bt ( ) Bt Bt Bt 21

23 2 Bt S Bt R SR RR Bt ( ) 21 ( ) ( ) 2-3 ( ) Bt Bt ( 1998) 3 Bt Bt+CpTI Cowpea trypsin inhibitor ( 321) Bt Bt (GKl2) ( 2000 )( 16) CpTI (blank cutworm Agrotis ypsilon Rottemberg) ( 2001 ) Bt + CpTI Beet armyworm Bt Bt Bt Bt 22

24 3 4 Bt 3 4 Bt Bt ( 1998) Bt 5 Bt LC 50 50% IC 50 50% µg/ml µg/ml (Wu et al 1999) Cotton pink bollworm, Pectinophora gossypiella Saunders Bt 23

25 Bt Vol. 31(4): , Vol.26(1): Bt Vol. 10(5): a Bt Vol. 1(2): b Bt Vol. 33(1): a Bt R93-6 Vol.43 (1): b Bt Vol.20(5): (Bt+CpTl) 2001 Bt (Cry1Ac) 2001 Bt (Cry1Ac) 1998 Bt Bt Vol. 41(1): Bt Wu, Kongming, Guo, Yuyuan and Nan LV, 1999, Geographic variation in susceptibility of Helecoverpa armigera (Lepidoptera: Noctuidae) to Bacillus thuringiensis lnsecticidal Protein in China, Journal of Economic Entomology, 92(2): Wu, Kongming, Yuyuan Guo, Nan Lv, John Greenplate, and Randy Deaton, 2002, Resistance 24

26 monitoring of Helicoverpa armigera (Lepidoptera: Noctuidae) to Bt insecticidal protein in China, submitted Bt (2000 ) 2001 Bt Vol.28 (1) : a Bt Vol 43 ( ) : b Bt Vol.33 (4) : Bt No.2:1-2( ) ( ) 2001 Bt Vol.12 (4) :

洋香瓜完稿.indd

洋香瓜完稿.indd 台南區農業改良場技術專刊 98-2 (NO.137) 台南區農業改良場編印 22 Contents 1 1 1 3 3 3 4 7 8 10 10 10 10 11 11 11 12 12 12 12 13 13 13 13 15 15 16 16 17 17 18 18 18 19 19 20 20 21 21 21 22 1086 95 6,241 14,655 96 4,432 13,467