图1 波纹龙虾呼吸系统组织学和组织化学 I Fig.1 Histologiy and histochemistry of the respiratory system in P. homaru I 1-1.丝鳃; 1-2. 鳃横切 23;1-3. 鳃横切模式图,示出鳃血管入鳃血管鳃轴和鳃丝整体图

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1 (Panulirus homarus) 2 Feulgen 6 (Panulirus homarus) Q Q A : (2006) (Panulirus homarus ) (Crustacea) (Decapoda) (Reptantia) (Palinuridae) (Panulirus) [1] Wright [2] Dehnel [3] [4] [5] [6] h 25~35 cm 200~400 g Carnoy's Bouin's 6~7µm H.E. [7] Olympus Carnoy's 6 [7 8] (1)Feulgen DNA, 60 1 mol/l HCl (2) - HgBpB (3) DMAB- (4) -Schiff -NH 2 (5)Adman -Alician (6)Million 2 [9 10] ( 1-1) ( ) sslyn@126.com ybcl@163.net 58 /2006 / 30 / 7

2 图1 波纹龙虾呼吸系统组织学和组织化学 I Fig.1 Histologiy and histochemistry of the respiratory system in P. homaru I 1-1.丝鳃; 1-2. 鳃横切 23;1-3. 鳃横切模式图,示出鳃血管入鳃血管鳃轴和鳃丝整体图; 1-4.入鳃血管局部横切图1-2 α部放大,示角质膜立方上皮结缔组织和扁平上皮 230; 1-5. 纵走隔膜图1-2β部放大,示鳃丝处纵走隔膜和入鳃血管裂开处 230; 1-6. 鳃腺图1-2γ部放大,示入鳃血管上方鳃腺 350 ;1-7. 微血管横切图1-2δ部放大,示4条微血管及x型结缔组织 230; 1-8. 鳃轴, 示鳃轴及鳃丝与鳃轴连接处 130; Av 入鳃血管 Cu 角质层 Ep 上皮 Ac 流入沟 Ga 鳃轴 Gf 鳃丝 Gg 腮腺 Ct 结缔组织 St 纵走隔膜 Cl 微血管 Ec 流出沟 As 入鳃血管裂开处 1-1. trichobranchia;1-2.transverse section of gill 23; 1-3. transverse section of gill; 1-4. transverse section of portion afferent vessel 230 ; 1-5. septum 230 ; 1-6. parotid 350; 1-7. transverse section of capillary 230; 1-8. axis 130;Av: afferent vessel ; Cu: cutin ; Ep: epithelial cell ; Ac: afflux channel ; Ga: gill axis ; Gf: gill filament ; Gg: gill bland ; Ct: connective tissue ; St: septum ; Cl: capillary vessel ; Ec: effluent channel ; As: afferent split Marine Sciences/Vol.30,No.7/

3 1 Tab.1 Branchial formula of P.homarus I II III I II III IV V -,- -,- -,- -,- -,- -,- -,- -,- -,- 1A,1Pl 1A,2Pl 1A,2Pl 1A,3Pl 1A,3Pl 1A,3Pl -,1Pl I~V n A Pl - (gill axis), 1-2, H.E ,1-3, , x 1-7 x , ,1-3 3 x , ~ , H.E. 60 /2006 / 30 / 7

4 也变深作用不明图2 波纹龙虾呼吸系统组织学和组织化学 II Fig.2 Histologiy and histochemistry of the respiratory system in P. homaru II 2-1. 鳃丝横切,示流出沟流入沟上皮和纵走隔膜 460 ; 2-2. 鳃丝末端纵切,示纵走隔膜不延续到鳃丝末端 230 ; 2-3. 鳃丝间转角处, 示流出沟过渡到流入沟的通路 230 ; 2-4. 特殊结构,示旋涡状结缔组织 transverse section of gill filament 460 ; 2-2. the end of filament 230 ; 2-3. transition between filaments 230 ; 2-4. unknow structure 组织化学与水之间因此气体交换就由其完成两层角质膜在组织化学测定结果见表 2 鳃丝处变薄内外层之间有空隙这样的结构既有利从表 2 可知角质膜的蛋白质含量较少而鳃丝于气体及离子等物质的交换又有利于鳃壁上皮细胞和血液中含量非常高除血液不含胱氨酸外其余几的自身保护[11] 血液从腹面的入鳃血管进入鳃丝通种结构中均含有酪氨酸色氨酸和胱氨酸过鳃丝上皮与鳃丝间流动的水进行气体交换鳃长轴 3 讨论与虾长轴垂直鳃丝在鳃轴两侧对生由于水流的 3.1 鳃丝功能提高了气体交换的效率这与其它甲壳动物的相同[4 方向是从背面流向腹面与血流方向相反从而大大每一条鳃丝由两层极薄角质膜扁平上皮和纵走 5] 且血液从入鳃血管到出鳃血管途中流经隔膜构成纵走隔膜和上皮层主要起分隔流入沟流条鳃丝大大延长了血液与水的相互作用时间这也出沟及支持鳃丝的作用由于其并非位于血液与水的增加了气体交换机会交界面估计不行交换气体的功能但上皮层还行使分泌角质膜功能鳃丝壁角质膜和扁平上皮处于血液 Marine Sciences/Vol.30,No.7/

5 2 Tab.2 Histochemistry on respiratory system of P.homarus DNA Feulgen mol/lhcl HgBpB NH 2 Schiff Million DMAB Adman -Alician [9] Carnoy's Bouin's [4 5] 3.4 [4 5] [4 5], [4 5] DNA DNA [1]. [J] [2] Wright D E. The structure of the gills of the Elasmobranch, Scyliorhinus canicula [J]. Z Zellforsch,1973,144: [3] Dehnel P A. Gill-tissue respiration in crab Eriocheir sinensis 62 /2006 / 30 / 7

6 [J]. Can J Zool,1974,52: [4]. [J]., [5]. [J]., 1995, [6]. [J]., [7]. [M]. : [8]. [M]. : [9]. [M]. :, [10]. [M]., [11]. [J]., Histological and histochemical studies on the respiratory system of Panulirus somaru SHI Shang-li, LI Chang-ling, CAO Fu-jun, HUANG Xiang-hu, LIU Chu-wu, LI Juan (Fisheries College, Guangdong Ocean University, Zhanjiang China) Received: Nov., 24, 2004 Key word : Panulirus homaru ; respiratory organ ; histology ; histochemistry Abstract: The respiratory organ of Panulirus homaru was studied with histological and histochemical methods. The results show that the superficial layers of gill are composed of two layers of cuticle membrane and simple cuboidal epithelium cells. Two lumens, which are respectively efferent branchial vessel above and afferent branchial vessel below are arranged in reverse side of gill. While gill axis that is formed by connective tissue is located at ventral side. Gill filament contains afferent channel,efferent channel and septum that is formed by connective tissue. Six methods had also been used to describe the functions of cutin membrane, epithelium, gill filament, gill axis, efferent branchial vessel and afferent branchial vessel. ( : ) Marine Sciences/Vol.30,No.7/

3 : 73 li rus hom arus ( Crustacea) (De2 capoda) ( Reptantia) ( Palinuridae) Panuli rus White [2 ],,,, [3 12 ],,,, 1 111, 25 35cm, g, 6 1h,,,,,

3 : 73 li rus hom arus ( Crustacea) (De2 capoda) ( Reptantia) ( Palinuridae) Panuli rus White [2 ],,,, [3 12 ],,,, 1 111, 25 35cm, g, 6 1h,,,,, 2 7 3 2 0 0 8 5 J OU RNAL OF TROPICAL OCEANO GRA P H Y Vol1 27,No. 3 May, 2 0 0 8,,, (, 524025) : Panuli rus homaru, ;,,, : Panuli rus homarus ; ; ; : Q174 ; S917 : A : 100925470 (2008) 0320072207 Histological

图1 波纹龙虾呼吸系统组织学和组织化学 I Fig.1 Histologiy and histochemistry of the respiratory system in P. homaru I 1-1.丝鳃; 1-2. 鳃横切 23;1-3. 鳃横切模式图,示出鳃血管 入鳃血管 鳃轴和鳃丝整体图

图1 波纹龙虾呼吸系统组织学和组织化学 I Fig.1 Histologiy and histochemistry of the respiratory system in P. homaru I 1-1.丝鳃; 1-2. 鳃横切 23;1-3. 鳃横切模式图,示出鳃血管入鳃血管鳃轴和鳃丝整体图