(Brassica oleracea L.) 1 2 3 7mMNO3-1mMNH4+ 1. 128 2. 3. (1895) Mayo (Wright and Davison, 1964) (hemoglobin methemoglobin) (cyanosis) (secondary amines) (nitrosamines) (Maynark 1989) et al., 1970 (Shaner and Boyer, 1976 Nicholas et al., 1976 Campbell and Smarrelli, 1978)
Nitrification 1992 1986 1986 Vogtmann et al., 1984 (1993) 120,000 1,900,000 367,135 (Rufty et al. 1982 (Rufty et al. 1984) (Maynark et al.,1970) 1992 1989 1995 [Kale (Brassica oleracea L. var. acephala DC.)] Johnson (Johnson et al., 1957) Table 1. The composition of nutrient solution for vegetable growing in the experiment Compound concentration KNO 3 Ca(NO 3 ) 2.4H 2 O NH 4 H 2 PO 4 6mM 4mM 2mM
MgSO 4.7H 2 O 1mM KCl 50µM H 3 BO 3 25µM MnSO 4.H 2 O 2µM ZnSO 4.7H 2 O 2µM CuSO 4.5H 2 O 0.5µM (NH 4 ) 6 Mo 7 O 24.4H 2 O(81 MoO 3 ) 0.5/7µM Fe-EDTA 20µM 20 60 45 3cm 3 1991 10 21:00 3:00,9:00,15:00,21:00 KCl CaCl 2 K + Ca 2+ 9:00 55 1. 0.2g 50mL 1N HCl 5mL 40mL 0.7M 1mL MnSO 4 50mL 100mg 30 2mL 46mL 1mL (diazoting 1mL reagent) (coupling reagent) 30 50ppm KNO 3 0.04 0.16ppm 540nm ( 1986) -- 100mL 2.4N HCl 0.5g sulfanilamide -- 100mL 0.12N HCl 0.3g N-(1-naphthyl-ethylene)diamine MnSO 4 --10g MnSO 4 51mL 1,000mL 2. 1NHCl 1.
20 15 30 25 1.0g 2.0g 1.0g 2.0g 2. 1.0g 0.6g PVPP(poly vinyl poly pyrroli-done) 6mL 30,000g 4 15 0.5mL 0.5mL 30 1mL 1 Sulfanilamide 1mL 0.2 N-(1-naphthylet hylene)diamine 30 540nm 50mM tris-hcl ph 7.8 15 Glycerol 14mM B-mercaptoethanol 1mM EDTA
0.1 Triton X-100 25mM phosphate buffer ph 7.5 5mM L-cysteine 10mM KNO 3 0.2mM NADH
Table 2. Reduced nitrogen concentration in kale Plant part Insoluble nitrogen Soluble nitrogen (mg/g DW) (mg/g DW) Blade 21.1±8.2 1.7±0.7 Petiole 7.5±3.0 1.9±0.6 Stem 8.6±2.2 2.1±0.6 Root 34.2±2.4 1.6±0.5 0.5 1.5mg 5 10mg
3 5mg 1 3mg
21.1mg 7.5mg
20 15 30 25 1.2
7mM NO - 3 1mMNH + 4 1. 1992 pp.15-27 2. 1986 61:205-211
3. 1995 (Brassica chinensis L.) 33:125-133 4. 1986 5. 1991 pp.151-171 6. 1995 7. 1993 8. 1989 146:34-41
9. 1989 148:36-46. 10. 1986 61:219-222 11.Campbell, W. H. and J. Smarrelli, Jr. 1978. Purification and kinetics of higher plant NADH: nitrate reductase. Plant Physiol., 61:611-616. 12.Johnson, C. M., P. T. Stout, T. C. Broyer and A. B. Carlton:. 1957. Comparative chlorine requirements of different plant species. Plant Soil, 8:337-353. 13.Maynard, D.N., A.V. Barker, P.L. Minotti and N.H. peck. 1970. Nitrate accumulation in vegetables. Adv. Agron., 28:71-118. 14.Nicholas, J.C., J.E. Harper and R.H. Hageman. 1976. Nitrate reductase activity in soybeans (Glycine max [L.] Merr.) Energy. limitations. Pl ant Physiol., 58:736-739. 15.Rufty, T.W. Jr., R.J. Volk, P.R. McClure, D.W. Israel and C.D. Raper, Jr. 1982. Relative content of NO 3 - and reduced N in xylem exudates as an indicator of root reduction of concurrently absorbed 15 NO 3-1. Plant Physiol., 69:166-170. 16.Rufty, T.W.Jr., D.W. Israel and R.J. Volk. 1984. Assimilation of 15 NO 3 - taken up by plants in the light and the dark. Plant Physiol., 76:769-775. 17.Shaner, D.L. and J.S. Boyer. 1976. Nitrate reductase activity in maize(zea mays L.)leaves. I. Regulation by nitrate flux. Plant Physiol., 58:499-504. 18.Shaner, D.L. and J.S. Boyer. 1976. Nitrate reductase activity in maize (Zea mays L.)leaves.. Regulation by nitrate flux at low leaf water potential. Plant Physiol., 58:505-509. 19.Vogtmann, H., A.T. Temperli, U.Kunsch, M. Eichenberger and P.Ott. 1984. Accumulation of nitrate in leafy vegetable grown under constrasting agricultural system. Biol. Agric. Hortic., 2:51-68. 20.Wright, M.J. and K.L. Davison. 1964. Nitrate accumulation in crops and nitrate poisoning in animals. Adv. Agron., 16:197-247.