,2017,37(12): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2017) 犱狅犻 : /j.issn "# 犘犘犗 $% &' ()*+ 1,2, 1,

,2017,37(12):2370-2377 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. :1000 4025(2017)12 2370 08 犱狅犻 :10.7606/j.issn.1000 4025.2017.12.2370 "# 犘犘犗 $% &' ()*+ 1,2, 1,3, 1, 1, 1, 2 (1 >, FG 471003;2,!"#$%&', HI 271018;3 JKLM, NO 466000), -: "PL2356QRS T U V W X Y(PPO) 4 Z 4 [ Z @ : \,] 2 3 PPO 4^_`a 犘犘犗 16 犘犘犗 29@ 犘犘犗 18,bc 7 "P3L4379 2 3 5 6 Q R4 Z :\[Z -:(1) 2AL $ 25 Z : 犘狆狅 犃 1 犪 ( PPO) 犘狆狅 犃 1 犫 ( PPO), : 51.5% 48.5% (2) 2DL $ 35 Z : 犘狆狅 犇 1 犪 ( PPO) 犘狆狅 犇 1 犫 ( PPO) 犘狆狅 犇 1 犪犫 ( ), : 57.8% 32.5% 9.8% (3) 2AL 2DL 4 $ Z 6 5 4 (: 犘狆狅 犃 1 犪 / 犇 1 犪 ( ) 犘狆狅 犃 1 犪 / 犇 1 犫 ( PPO) 犘狆狅 犃 1 犪 / 犘狆狅 犇 1 犪犫 ( ) 犘狆狅 犃 1 犫 / 犇 1 犪 ( PPO) 犘狆狅 犃 1 犫 / 犇 1 犫 ( ) 犘狆狅 犃 1 犫 / 犘狆狅 犇 1 犪犫 ( ),,@ PPO 4 ( 犘狆狅 犃 1 犫 / 犇 1 犪 4 25.6% (4)2 3 PPO Z " P L 4:\ - [Z, 犘狆狅 犃 1 犫 3L 3L4, 犘狆狅 犇 1 犪 J 3L 3L4, ( 犘狆狅 犃 1 犫 / 犇 1 犪 3L4, ( :;`a (MAS), ] 23ST 64ABCD 8 5./0: 23;"PL;PPO;^_`a;MAS 1 ( :Q789;S512.1 :A 犌犲狅犵狉犪狆犺犻犮犇犻狊狋狉犻犫狌狋犻狅狀狅犳犃犾犲犾犻犮犞犪狉犻犪狋犻狅狀狅犳犘狅犾狔狆犺犲狀狅犾犗狓犻犱犪狊犲 ( 犘犘犗 ) 犌犲狀犲犻狀犠犺犲犪狋犆狌犾狋犻狏犪狉狊犳狉狅犿犇犻犳犳犲狉犲狀狋犈犮狅犾狅犵犻犮犪犾犚犲犵犻狅狀狊 WANGLiming 1,2,GAO Huali 1,3,LIYongxia 1,DONGPuhui 1, WANGChunping 1,LIXingfeng 2 (1AgronomyColege,HenanUniversityofScienceandTechnology,Luoyang,Henan471003,China;2StatekeyLaboratoryof CropBiology,AgronomyColege,ShandongAgriculturalUniversity,Tai an,shandong271018,china;3huangfanqufarmin HenanProvince,Zhoukou,Henan466000,China) 犃犫狊狋狉犪犮狋 :StudyondistributionofPPO geneactivityindiferentwheatecologicalregioncultivarswould benefitscreeningelitegermplasmsforimprovingwheatquality.atotalof379wheatcultivarsweregeno typedbythefunctionalmarkers 犘犘犗 16, 犘犘犗 29and 犘犘犗 18.Theresultsindicatedthat:(1)Therewere twotypesofppogeneson2alchromosomes: 犘狆狅 犃 1 犪 (highppo)and 犘狆狅 犃 1 犫 (lowppo),andtheir frequencywere51.5%and48.5%,respectively.(2)therewerethreetypesofppogeneson2dlchromo :2017 09 01; :2017 10 23 : (U1304318);! " # $ % & ' (2008KF01); (162300410077); (!)*(172102410052)!:+,-(1976-),.,/0,1, 23456789:; 5! E mail:lmwang1@163.com! : <=,/0,, ">?@23ABCD4E @ E mail:lixf@sdau.edu.cn

12 +,-, :23 PPO Z4LX:\ 2371 somes: 犘狆狅 犇 1 犪 (lowppo), 犘狆狅 犇 1 犫 (highppo)and 犘狆狅 犇 1 犪犫 (intermediatetype),andtheirfrequen cywere57.8%,32.5% and9.8%,respectively.(3)thereweresixdiferentgenecombinationsonthe chromosomeof2aland2dl: 犘狆狅 犃 1 犪 / 犇 1 犪 (intermediatetype), 犘狆狅 犃 1 犪 / 犇 1 犫 (highppo), 犘狆狅 犃 1 犪 / 犘狆狅 犇 1 犪犫 (intermediatetype), 犘狆狅 犃 1 犫 / 犇 1 犪 (low PPO), 犘狆狅 犃 1 犫 / 犇 1 犫 (intermediatetype)and 犘狆狅 犃 1 犫 / 犘狆狅 犇 1 犪犫 (intermediatetype).thefrequencyof 犘狆狅 犃 1 犫 / 犇 1 犪 (low PPO)was25.6%.(4)The distributionfrequencyaboutdiferentvariationtypesofwheatppogeneinvariousecologicalregionsexis tedobviousdiferences:theproportionofthegenotypeof 犘狆狅 犃 1 犫 waslargerinnorthernwinterwheatre gionandsouth westernwinterwheatregion,and,theproportionofthegenotype 犘狆狅 犇 1 犪 waslargerin YelowandHuaifacultativewinterwheatareaandnorthernwinterwheatregion.Theproportionofgeno typecombination 犘狆狅 犃 1 犫 / 犇 1 犪 waslargerinnorthernwinterwheatregion.therefore,molecularmarker assistedselection (MAS)methodcouldbeconsideredforgeneticimprovementofappearancequalityof wheatgrain,andthroughintroducingfinegermplasmfromthenorthernwinterwheatregion. 犓犲狔狑狅狉犱狊 :commonwheat;ecotype;ppo;functionalmarker;mas 23 # 4!, U 4,23 K5, # 423 " 64, ; B 4 6 4 23 64# `, 23 6ABCD4# * ` [1] 2 3 4 U V W X Y (polyphenol oxidase,ppo)_ ( ) ", 4 [2] PPO 423 5( ) C D 4 [3 4] PPO 4ST PPO 43%, 4 50% ~ 70% [5] 23 PPO @, 4!, 23 5 PPO 4[ Z PPO 4 Z [6] 23 PPO 4!2 R", #,! 5 R " 6B 7D $ % PPO 4& [7] ' [8],% 23ST PPO 4 2AL 2DL, : 37.2% ~50.1% 25.1%~29.1%4 Z Sun [9] ] 2A 4 (GenBank ( AY596268) ) STS^_`a 犘犘犗 18,bc 犘狆狅 犃 1 $4 Z He [10] *+2D 4,-(GenBank ( AY515506) 2./ 4 STS(SequenceTagSite)^ _ ` a,: 犘犘犗 16 犘犘犗 29, L : 犘狆狅 犇 1 $ 4 Z 0(] 14 `a bc 2 356 PPO 4 Z [11 13],] 4 : ^ _ ` a [14] 8 2 [15 16] 3 [17 18] [19] J 3 L [20] 45 [21 23] 67 [24] U (8L)423 PPO Z 9, : ; U "PL( ), ] 1 4 :`a PPO $ bc, 0(] U ^_`a # U 23"PL 9 PPO Z9 :\4 < = 0 ( ] STS ^ _ ` a 犘犘犗 16 犘犘犗 29 犘犘犗 18 3 ^ _ ` a, 7 3L4 379 2356 :;bc,- > 犘狆狅 犃 1@ 犘狆狅 犇 1? $4 Z, "PL4:\@A :B, PPO 2356QR4C, D D2356 EE 1 :; 1.1 2 3 E 23 5( ) 7 23 L, ) 379 :;( 1) :;F R > 23GH, 2014~2016 5 > M,I J E F K L @ 1.2 4 5 1.2.1 犇犖犃 67 M E :; N 3 T O 45;, N 8 4, P CTAB [25] N DNA, Q R S T U V W >?bc DNA 6 1.2.2 犛犜犛 8 ] 23 PPO 2A 2D $4 STS ^_`a 犘犘犗 16 犘犘犗 29 犘犘犗 18bc "PL23 4

2372 37 91 :;"# ( ) < ()=> Table1 WheatcultivarscolectedfromdiferentecologicalregionsofChina OY Code 23" L Wheatecologicalregion 5( ) Wheatcolection Ⅰ J 3L Huanghuaiwinterwheatregion(HHWWR) 251 Ⅱ 3L Northernwinterwheatregion(NWWR) 41 Ⅲ 3L Southwesternwinterwheatregion(SWWWR) 53 Ⅳ 3L Changjiangmiddle lowerreacheswinterwheatregion(cmwwr) 25 Ⅴ Z 3L Qingzangspringwinterwheatregion(QSWWR) 4 Ⅵ Z3L Northeasternspringwheatregion(NESWR) 3 Ⅶ Z3L Northwesternspringwheatregion(NWSWR) 2 92 "#2 犃?2 犇 @ABC 犘犘犗 &DE 8 Table2 PrimersforidentificationofPPOgenesonchromosomes2Aand2Dincommonwheat `a Marker [,- Primersequence(5 3 ) \] Forward ^] Reverse _` a Fragmentsize/bp *` Z Targetedalele 犘犘犗 16 TGCTGACCGACCTTGACTCC CTCGTCACCGTCACCCGTAT 713 犘狆狅 犇 1 犪 犘犘犗 29 TGAAGCTGCCGGTCATCTAC AAGTTGCCCATGTCCTCGCC 490 犘狆狅 犇 1 犫 犘犘犗 18 AACTGCTGGCTCTTCTTCCCA AAGAAGTTGCCCATGTCCGC 685/876 犘狆狅 犃 1 犪 / 犘狆狅 犃 1 犫 Z `a4[b c" " >? ( ( 2) 1.2.3 犘犆犚 F G H I J PCR ^ 20 μl,20mmol L -1 Tris HCl(pH8.4),20mmol L -1 KCl,200μmol L -1 dntps,1.5 mmol L -1 MgCl 2,M [0.01μmol,TaqDNA (Y l.5u, DNA80ng ^ T100 TM Thermal Cycler (Bio Rad), Touchdown PCR,, 95 5min;95 30s,66 ~54 30s (M 0.3 ),72 1 min,40 ;72 5min;4 _` 0.08kg L -1 4 TUV W: bc, 1 TBE,110V V VW3h(*+_` a 2 9 VW# ),, Tanon Gis 2010 TU *+M :;4bc PPO, :B 2 @:B 2.1 KL < " # 犘狆狅 犃 1 M ' & N () 犘犘犗 18 ` a 379 E : ;,) b c 犘狆狅 犃 1 犪 ( PPO ) 犘狆狅 犃 1 犫 ( PPO ) 25,_` : 685bp 876bp a( 1),, 犘狆狅 犃 1 犪 $ 4 : ; 195,51.5%; 犘狆狅 犃 1 犫 4:;184, 48.5%,b,E :; PPO 4 : ; U( 3) b 2,A @ 3, 犘狆狅 犃 1 犪 犘狆狅 犃 1 犫 4 Z "PL4:\,,J 3L Z 犘狆狅 犃 1 犪 (55.0%)4:; U Z 犘狆狅 犃 1 犫 (45.0%); 3L Z 犘狆狅 犃 1 犪 (41.5%)4 : ; Z 犘狆狅 犃 1 犫 (58.5%) @ PPO Z 犘狆狅 犃 1 犫 3L 3L4,: 58.5% 60.4%; 3L Z 3L4:;,@ PPO 4 Z 犘狆狅 犃 1 犪,: 56.0% 75.0% 2.2 KL < O " # 犘狆狅 犇 1 M ' & N () 犘犘犗 16`a 犘狆狅 犇 1 犪 ( PPO ) 4:; _` 713bp a, 犘狆狅 犇 1 犫 ( PPO ) 4:; PCR _` a( 3,A); ^, 犘犘犗 29 ` a 犘狆狅 犇 1 犫 4 : ; _ ` 490bp 4 a, 犘狆狅 犇 1 犪 4 : ; PCR _ ` a ( 3,B), 2 ` a b c #,./,. & 379 :; 2DL $ % PPO Z:B,b c 犘狆狅 犇 1 犪 犘狆狅 犇 1 犫 犘狆狅 犇 1 犪犫 3 5 Z, PPO 犘狆狅 犇 1 犪 4:;) 219 U,

12 +,-, :23 PPO Z4LX:\ 2373 M.DL2000;1. 58;2. 3217;3. 1(;4. 3202;5. 6(;6. 311(;7.I06 16;8.3301; 9. 312(;10. 8(;11.332;12. 64 1 犘犘犗 18bc "PL : 23 犘狆狅 犃 1 $4 Z M.DL2000;1.Aikang58;2.Bainong3217;3.Heyu1;4.Dengmai202;5.Huapei6;6.Fengdecunmai11; 7.An06 16;8.Guomai301;9.Fengdecunmai12;10.Huapei8;11.Guomai32;12.Bainong64 Fig.1 Alelicvariationof12wheatcultivarsfromdiferentecologicalregionsbythemarkerof 犘犘犗 18for 犘狆狅 犃 1 93 "#KL 犘狆狅 ' & ( ) KL < () Table3 Distributionofwheatdiferent 犘狆狅 genes(combinations)indiferentwheatecologicalregions Genetype )*Itern "PL Wheatregion Ⅰ Ⅱ Ⅲ Ⅳ Ⅴ Ⅵ Ⅶ F Mean 犘狆狅 犃 1 犘狆狅 犇 1 犘狆狅 犃 1 / 犘狆狅 犇 1 犃 1 犪犃 1 犫 ( Total 犇 1 犪犇 1 犫犇 1 犪犫 ( Total 犃 1 犪犇 1 犪犃 1 犪犇 1 犫犃 1 犪犇 1 犪犫犃 1 犫犇 1 犪犃 1 犫犇 1 犫犃 1 犫犇 1 犪犫 ( Total 5( ) NO.ofcultivars 138 17 21 14 3 1 1 195 Frenquency/% 55.0 41.5 39.6 56.0 75.0 33.3 50.0 51.5 5( ) NO.ofcultivars 113 24 32 11 1 2 1 184 Frenquency/% 45.0 58.5 60.4 44.0 25.0 66.7 50.0 48.5 5( ) NO.ofcultivars 251 41 53 25 4 3 2 379 Frenquency/% 100 100 100 100 100 100 100 100 5( ) NO.ofcultivars 146 29 25 14 2 1 2 219 Frenquency/% 58.2 70.7 47.2 56.0 50.0 33.3 100 57.8 5( ) NO.ofcultivars 74 10 26 10 2 1 0 123 Frenquency/% 29.5 24.3 49.1 40.0 50.0 33.3 0.0 32.5 5( ) NO.ofcultivars 31 2 2 1 0 1 0 37 Frenquency/% 12.4 4.0 3.7 4.0 0.0 33.3 0.0 9.7 5( ) NO.ofcultivars 251 41 53 25 4 3 2 379 Frenquency/% 100 100 100 100 100 100 100 100 5( ) NO.ofcultivars 88 12 9 10 2 0 1 122 Frenquency/% 35.1 29.2 17.0 40.0 50.0 0.0 50.0 32.2 5( ) NO.ofcultivars 33 4 12 3 1 0 0 53 Frenquency/% 13.1 9.8 22.7 12.0 25.0 0.0 0.0 14 5( ) NO.ofcultivars 17 1 0 1 0 1 0 20 Frenquency/% 6.8 2.4 0.0 4.0 0.0 33.3 0.0 5.3 5( ) NO.ofcultivars 58 17 16 4 0 1 1 97 Frenquency/% 23.1 41.4 30.2 16.0 0.0 33.3 50.0 25.6 5( ) NO.ofcultivars 41 6 14 7 1 1 0 70 Frenquency/% 16.3 14.6 26.4 28.0 25.0 33.3 0.0 18.5 5( ) NO.ofcultivars 14 1 2 0 0 0 0 17 Frenquency/% 5.6 2.4 3.8 0.0 0.0 0.0 0.0 4.4 5( ) NO.ofcultivars 251 41 53 25 4 3 2 379 Frenquency/% 100 100 100 100 100 100 100 100 :Ⅰ~Ⅶ 1, Note:Ⅰ-Ⅶ sameastable1,thesameasbelow

2374 37 2 23 犘狆狅 犃 1(A)@ 犘狆狅 犇 1(B)9 犘狆狅 犃 1/ 犘狆狅 犇 1(C) ( "PL4:\ Fig.2 Distributionofthegenesof 犘狆狅 犃 1(A), 犘狆狅 犇 1(B)and 犘狆狅 犃 1/ 犘狆狅 犇 1(C)indiferentwheat ecologicalregioncultivars M.DL2000;1. 58;2. 3217;3.I06 16;4.3301;5.332;6. 1(;7. 64;8. 3202; 9.K35(;10. 5(;11. 6(;12.K38( 3 犘犘犗 16(A) 犘犘犗 29(B)bc "PL : 23 犘狆狅 犇 1 $4 Z M.DL2000;1.Aikang58;2.Bainong3217;3.An06 16;4.Guomai301;5.Guomai32;6.Heyu1;7.Bainong64; 8.Dengmai202;9.Fanmai5;10.Huapei5;11.Huapei6;12.Fanmai8 Fig.3 Alelicvariationof12wheatcultivarsfromdiferentecologicalregionsbymarkers of 犘犘犗 16(A)and 犘犘犗 29(B)for 犘狆狅 犇 1 57.8%; PPO 犘狆狅 犇 1 犫 4:;) PPO 4 犘狆狅 犇 1 犫 3L 123, 32.5%; # bc 37 Z #, 70.7%, - 8 L # 2 3 ( 犘狆狅 犇 1 犪犫 4:;, 9.8%;@ 犘犘犗 18 bc, 犘犘犗 16+ 犘犘犗 29 b c PPO b 2,B @ 3 : B,@ 64ABCD; J 3L 3 L:\ $,: 58.2% 56.0%; ( 犘狆狅 犇 1 犪犫 J 3L:\ U,) 31

12 +,-, :23 PPO Z4LX:\ 2375 :;, ( :;483.8% 2.3 KL <O "# 犘狆狅 犃 1? 犘狆狅 犇 1 M' & N () 3 STS ^ _ ` a 犘犘犗 16 犘犘犗 29 犘犘犗 18 379 :;4:B, "PL23 PPO 2A 2D 4 $ Z,) 6 5 Z (,: : 犘狆狅 犃 1 犪 / 犇 1 犪 ( PPO ) 犘狆狅 犃 1 犪 / 犇 1 犫 ( PPO ) 犘狆狅 犃 1 犫 / 犇 1 犪 ( PPO ) 犘狆狅 犃 1 犫 / 犇 1 犫 ( PPO ) 犘狆狅 犃 1 犪 / 犘狆狅 犇 1 犪犫 ( PPO ) 犘狆狅 犃 1 犫 / 犘狆狅 犇 1 犪犫 ( PPO ), : 32.2% 14.0% 25.6% 18.5% 5.3% 4.5% C 3 366 64 332 PPO $ 犘狆狅 犃 1 犫 / 犇 1 犪 (456QR 3@ 2,C,PPO Z ( "PL:\ [Z,,J 3L 3L23,PPO Z ( 犘狆狅 犃 1 犪 / 犇 1 犪,: 35.1% 40.0%; 3L 3L23 PPO Z ( 犘狆狅 犃 1 犫 / 犇 1 犪,: 41.5% 30.2% 3 23ST PPO 23 4#, "PL23 5( )PPO Z 9 :\, 23 PPO = (4 9 O PPO 4 =,E 2 3 : ; PPO 犘狆狅 犃 1 犫 犘狆狅 犇 1 犪 犘狆狅 犃 1 犪 / 犘狆狅 犇 1 犪 4 : 48.5% 57.8% 25.6% [26] -, 2 3 犘狆狅 犃 1 犫 犘狆狅 犇 1 犪 2 3 5 ( ) F,: 58.2% 59.8%, PPO ( 犘狆狅 犃 1 犪 / 犘狆狅 犇 1 犪 $ 32.5%? [ Z _ %& :; R9,= 56 b R 7 "PL4 423 59U 5 C 4 O, [26] :;b 4 23"PL4 5@ 5205 9 ( ) E4L =106, 17 U D 6 2 3 8 5, E4L D6 = 23 PPO 4# = PPO $ 23 "P L:\@A :B, "PL PPO $4:\ [Z-, "PL $ :\ $ [Z, @ [11,20,25] ;=, Z 3L 3L423 5 PPO $ -, 5[ _@ 84"P, "PL 423 5( )PPO 8"P 4 5 ; 3L 3L423 5( ) PPO $( () F, "PL23 5( )4 PPO _ 8 8 4 ;,$ 23 5! # 23 CD4, 5 (E 5*`,C DZ = (E (, *+ 2 3 5 " P L ( [2016]1( ), 8 PPO 23 5( )4 = ] `a 犘犘犗 16 犘犘犗 29 犘狆狅 犇 1 $bc,e :; 犘狆狅 犇 1 犪 犘狆狅 犇 1 犫 犘狆狅 犇 1 犪犫 ) 3 5 Z, 犘狆狅 犇 1 犪犫 8, 4, :;) 37, 31 J 3L; 4 _@ 5 4 5 ( 5 5 (, :;`a ( *4,23 PPO 2 3 2A 4 犘狆狅 犃 1 @ 2D 4 犘狆狅 犇 1 $%, 2B 4 U QTL $ 4 [27 32], :;`a C PPO 423 5 ( ), 0(] PPO 4^_ `a = ] PPO 4 ^ _ ` a 犘犘犗 16 犘犘犗 18 犘犘犗 29 E : ; 犘狆狅 犃 1 犘狆狅 犇 1 $4 Z,C U PPO $( ()4D6 5( ), 23 CD E K4:;, `a :;`a 5 4 #,! _ ( 23 O:;4 PPO " c, #_$ 4% @ 54&> ;', @ 4 9 @8X U 40(

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