http://www.autoprep.jp ELISA ELISA ELISAenzyme-linked immunosorbent assay
ELISA ELISA GC-ECD ELISA
ELISA ELISA ELISA ELISA ELISA ELISA
ELISA ELISA Antibody coated microwells) (Mycotoxin standard ( (non-coated dilution microwells) ELISA ELISA (Mycotoxin conjugate) (Substrate and Stop solution) 200150 DON 100 No.1 (TMB) ELISA 100L 200L1L 100-200L (450nm) ELISA
28 4 30 18
DON L w/v L 1
L 3
MP Romer Labs Aflatoxin (1-20ppb) QUANTITATIVE KIT Data Reduction Worksheet Operator: TLM Date: 6-Nov-02 Assay ID: Analysis of soy samples Plate Lot#: SIN-02-0053 Conj. Lot: SIN-02-0054 Stds Lot: SIN-02-0046, 0055-0059 Substrate Lot: SIN-02-0078 Stop Lot: SIN-02-0079 BioRad Reader Section I: Calibration Curve Std. Level Abs. B/Bo Log(Conc.) Logit B/Bo 0 ppb = 2.274 1.0 ppb = 2.039 0.90 0.94 R^2 = -0.9949 2.5 ppb = 1.749 0.77 0.40 0.52 Slope = -1.1915 5.0 ppb = 1.363 0.60 0.70 0.17 Intercept = 0.9615 10.0 ppb = 0.727 0.32 1.00-0.33 20.0 ppb = 0.501 0.22 1.30-0.55 1.20 1.00 0.80 0.60 0.40 0.20 0.00-0.200.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40-0.80-0.60-0.40 logit B/Bo Avg. ppb 1 0 ppb std 1.0 ppb std 2.5 ppb std 5.0 ppb std 10.0 ppb std 20.0 ppb std Sample 3 mixed Sample 3 mixed Sample 1 Sample 2 Sample 3 Sample 4 Sample 1 spiked 10 ppb total Afla Sample 2 spiked 10 ppb total Afla Sample 3 spiked 10 ppb total Afla Sample 4 spiked 10 ppb total Afla Sample 1 Sample 2 Sample 3 Sample 4 Sample 1 spiked 10 ppb total Afla Sample 2 spiked 10 ppb total Afla Sample 3 spiked 10 ppb total Afla 2.274 #DIV/0! #DIV/0! No. ABS SAMPLE ppb Stdev 3 1.749 0.52 2.34 2 2.039 0.94 1.05 4 1.363 0.17 4.57 6 0.501-0.55 18.52 5 0.727-0.33 12.09 7 8 1.584 0.36 3.19 10 9 2.405 #NUM! #NUM! 11 1.881 0.68 1.72 12 2.207 1.52 0.34 13 2.178 1.36 0.47 14 1.741 0.51 2.37 15 0.547-0.50 16.83 16 0.605-0.44 15.03 17 0.597-0.45 15.26 19 18 0.532-0.52 17.35 20 2.181 1.37 0.45 21 2.261 2.24 0.08 22 2.213 1.56 0.31 23 1.462 0.26 3.91 24 0.617-0.43 14.69 25 0.546-0.50 16.86 26 0.643-0.40 14.01 Section II: Sample Calculations Log(Conc.) Logit(B/B0)
Afla Afla Aflatoxin B1 B2 G1 G2 GIPSA Afla 4/40 (Lot# SIN(A)01-0001) 104 50g Aflatoxin B1, B2, G1, G2 (ppb)20 31 1 100 11.6527.4610042.4% Afla Afla 41 4 GIPSA 414) 1 2 50g 1 1. 3 2. 4421371 Afla 41 4 GIPSA 414) 1 2 50g 1 1. 3 2. 4421371 Afla GIPSA 1 (Lot# SIN(A)01-0001) 6 10 50g 3 31 334 13
Afla Afla DON ELISA TLC 0.50.7 GC-ECDAOAC 0.9 HPLC 0.9 DON DON CV 0.9976 0.9983 0.9978 5.7 1.2 4.3 n=105 n=81 48.1 40.3 44.7 1 20.2 16.7 18.7 17.1 9.5 14.2... 80.1 78.4 79.4 105 105 105 99.4 98.2 99.5
n=178 DON CV 1 18.8 23.2 31.7 21.4 16.6 19.6 25.9 13.0 CV 1 11.7 24.8 16.1 11.7 23.4 15.5 17.0 11.0 n=159 n=85... 91.3 96.0 84.9 93.0 97.3 77.2 73.2 96.8 91.4 85.0 105 94.2 97.1 73.0 83.0 106 n=40 n=15 ND False posi. 3.4 0 False posi. 9.4 0 False nega. 13 1.2... n=159 n=85 ND False posi. 2.5 0 False posi. 0.0 0 False nega. 10 0 n=40 n=15 n=83 false negative CV 13.2 A B C 15.2 10.5 15.2 11.0 16.2 9.1 12.8 11.1 11.6 10.3 13.5 A B C 118 124 110 A B C ND False positive 0 6.7 0 2.3 False positive 0 0 0 0 False negative 0 0 0 0 False negative 0 0 0 0 118 107 112 105 108 121 117 99.9 113 CV A B C 5 3 2 4.1 19.6 7.4
CV 1 16.5 21.9 19.4 21.1 25.0 40.6 13.8 18.8 17.2 19.1 20.9 32.4 n=80 9 19 Afla Afla 4mL 30 0.6mL MycoSep #112 ELISA // //