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1 3

1. 2. 3. 4. 5. 4

6. VAC Amp 1 100 0.375 2 115/120 0.75 3 220/240 3 230v 5

1. 2. 3. 4. 5. 1. HPLC 0.45 6

1.4! * PEEK (THF) (DMSO) PEEK 0.5%( ) 7

1. ( ) ( ) 2. * * 1 2 3 8

1 * * * (10-10 S/m ) ( ) 2 ( ) (1) 18 4 ( 3 3mm ) 3 * * ( 1M * * 9

4 2mm * 10 20 5 * ( ) (10-10 S/m ) * * 65% ( ) ( ) * HPLC ( 0.45µm ) * (SUS316) KCI NaCI NH 4 CI 10

* SPD-500 UV UV HPLC * * 2. 3. 4. 5. 6. 7. 8. 11

2 12

LC-500A 1. 7725i LC-500A 13

LC-500HPLC 14

2. 1. LC-500A LC-500HPLC 2. LC-500A LC-500A 7725i 15

LC-500A HPLC 0.8% (0.1ml/min~10ml/min) 6000pasi 42cm30cm20cm 8kg 110~240v47~63HZ80w 60 20 LC-500A GLP/GMP 16

1 1

2.3 LC-500A 2 2

2.3 LC-500A 1 2 3

1. LC-500A 1/8 PEEK Fitting 1.6F 10 Micron 1 1 1 1 1 1/8 *48 FEP 1 1/16 PEEK Fitting 9/64 T 2. 1 1 1 1 1/4 WK500-T-1401 1 1/2 () WK500-T-1201 1 9/64 WK500-T-9640 1 1 WK500-T-9640 1 4

2 WK500-T-501 1 WK500-T-502 1 WK500-T-503 1 1 1 3. 5 ul WK500-4000005 WK500-5003111 10 ul WK500-4000010 WK500-5003111 25 ul WK500-4000025 WK500-5003111 50 ul WK500-4100050 WK500-5003112 150 ul WK500-4100150 WK500-5003113 250 ul WK500-4100250 WK500-5003113 350 ul WK500-4200350 WK500-500-4113 500 ul WK500-4200500 WK500-500-5113 4 WK500-500-0010 WK500-500-0011 WK500-500-0012 WK500-500-0013 5

5 WK500-5003010 WK500-5001005 WK500-5001004 WK500-5001003 WK500-5001025 6 WK500-500-1601 ID1.6 WK500-500P-1601 PEEK PEEK WK500-500P-1602 ID1.6 7 WK500-500-1610 ID1.6 WK500-500-2001 ID2.0 WK500-500P-1603 PEEK ID1.6 6

8 316 WK500-GL316 PEEK WK500-GL01 OD1.6ID0.17 PEEK WK500-GL02 OD1.6ID0.12 WK500-GL03 OD1.6ID0.5 WK500-GL04 OD1.6ID0.5 WK500-GL05 OD1.6ID0.3 WK500-GL06 OD3.0ID2.0 7

3 8

6050cm 20kg 210~240v! 1 HPLC 2 HPLC 3 4! 5 6 7 * 4--35 * * * * 45-85% 9

:,,,! 60CM 10cm 10

210~240V VAC Amp 1 100 0.375 2 115/120 0.75 3 220/240 4 210 240v 1. * 1).0 2). 3). 4). 11

3.2 2.! * * * * *! OFF 1) 2) 3! 12

HPLC LC-500A LC-500A PEEK 1 316 OD:1.6mm ID:0.3m PEEK OD1.6 mm ID0.12m OD1.6mm ID0.17m OD3.0 mm ID2.0m 13

OD1.6 mm ID0.5m 1.6 3.0 PEEK 1.6 PEEK 3/16 32 PEEK PEEK 1/4 1/8 14

2 1. 2. 1. PEEK 2. a. 15

b. c. HPLC 3 ------ -- --------------------- ------------ 16

3.3 1. 2. 3. 1/4 1/8 PEEK PEEK 17

3.3 * SUS * PEEK 4 * PEEK *7725i 18

HPLC RHeodyne 7725i PEEK 6 1~4 2 3 56 7725i 7725i 5 1. ) 0 19

2. ) 7725i 3. ) LC-500AG LC-500A RS-232 A 230v 1 2 20

B C 21

D SUS 22

B 230v 1 2 B 23

C D SUS 24

4 25

1 LED 26

/ 1 Press XXXX Psi 2 Flow 01.000 ml/min 3 Pmin 0000 Pmax 5400 4 100 solvent H 2 O 5 Run 6 off 4.1 2 19 27

0-9 Enter Run Stop Flow P/min P/max Activate edit Purge / 180 1 I, O 2 I 28

3 29

1. (/) Pressure 0000psi Head Type ANALYTICAL Flow 00.000ml/min flow Local Pmin 0000 pmax 5400 121 1000 197 097 167 Solvent IPA p. Edit Mthd00 Step00 Link00 Flow 00.000ml/min Slvnt A IPA B H2O Too 000.2 A 100 B000V T01 000.4 A 000 B100V Modify Method 000 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods edit p. 30

1. 09.999ml/min 0.001ml/min 0ml/min 05400psi 1psi 0000psi 06000psi 1psi 5400psi 06000psi 1psi 0psi * 1kgf/cm 2 =0.098MPa=0.98bar=14.2psi 1 Head Type ANALYTICAL Local 121 1000 197 097 2 Pressure 0000 psi Flow 00.000 ml/min Pmin 0000 Pmax 5400 31

4.4 3 flow Pressure 0000 psi Flow 01.000 ml/min Pmin 0000 Pmax 5400 167 Solvent IPA 4 Pressure 0000 psi Flow 01.000 ml/min Pmin 0200 Pmax 3000 167 Solvent IPA 5 Pressure 0000 psi Flow 01.000 ml/min Pmin 0200 Pmax 3000 167 Solvent IPA Pressure 0000 psi Flow 01.000 ml/min Pmin 0200 Pmax 3000 167 Solvent IPA 32

4.4 Pressure 0000 psi Flow 00.000 ml/min Pmin 0000 Pmax 5400 167 Solvent IPA 1 Pressure 0000 psi Flow 00.000 ml/min Pmin 0000 Pmax 3000 167 Solvent IPA 2 Pressure 0000 psi Flow 00.000 ml/min Pmin 0000 Pmax 5400 167 Solvent IPA 33

4.4 1 Pressure 0000 psi Flow 00.000 ml/min Pmin 0000 Pmax 5400 167 Solvent IPA 2 Pressure 0000 psi Flow 00.000 ml/min Pmin 0100 Pmax 5400 167 Solvent IPA 34

LC-500A +/-2.5% +/-1.0% 1 H2O 100 9 NHPTN 200 2 IPA 167 10 BTNL2-2 190 3 Aceto 195 11 ISBTL 180 4 MeOH 185 12 BTNL1-1 190 5 THF 160 13 BTLAC 170 6 STD 125 14 DMTNS 167 7 PPNL2 2-167 15 CAN 180 8 AGN 138 16 S16-S29 35

1 Pressure 0000 psi Flow 00.000 ml/min Pmin 0100 Pmax 5400 167 Solvent IPA 2 Pressure 0000 psi Flow 00.000 ml/min Pmin 0100 Pmax 5400 185 Solvent 36

1 2 3 4 5 6 7 37

4.6 8 Pressure 0000 psi Flow 02.000 ml/min Pmin 0100 Pmax 5400 185 Solvent MeOH 9 10 11 1 38

4.6 2 3 4 5 39

1 2 Pressure 0000 psi Flow 01.000 ml/min Pmin 0100 Pmax 5400 185 Solvent MeOH 3 4 Pressure 0458 psi Flow 01.000 ml/min Pmin 0100 Pmax 5400 185 Solvent MeOH 5! 40

1 2 Modify Method 05 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods 3 Mthd02 Step05 Link00 Flow 01.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 42

Flow Flow Enter Enter Head Type ANALYTICAL Local edit Modify Method 00 Copy Method 00 to 00 Delete Method 00 Enter Mthd01 Step00 Link00 Flow 01.000 ml/min Slvnt A IPA B H2O Flow Enter Mthd01 Step00 Link00 Flow 01.000 ml/min Slvnt A MeoH B H2O Edit Mthd01 Step00 Link00 Flow 01.000 ml/min Slvnt A MeoH B H2O Enter Enter Mthd01 Step00 Link00 Flow 01.000 ml/min Slvnt A MeoH B H2O T00 010.0 A090 B010V Edit 43

: 1. Modify Method 00 1. edit Copy Method 00 to 00 Delete Method 00 Clear ALL Methods 2. edit Modify Method 01 Copy Method 00 to 00 Delete Method 00 2. 1~59 enter Clear ALL Methods 1 Enter 2. Mthd01 Step00 Link00 Flow 1. Step 01.000 ml/min Slvnt A IPA B H2O T00 004.0 2. enter 0 A100 B000V Mthd01 Flow Slvnt Step00 01.000 A IPA Link01 ml/min B H2O 1.0ml/min 10 10ml/min T00 004.0 A100 B000V 44

3. 1. link field Mthd01 Step00 Link01 Flow 02.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 2.enter 4. 1. flow 2.enter Mthd01 Step00 Link01 Flow 02.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 5. 1. Slvnt A field 2. 3. Slvnt B field 4. * A B 45

5. 20 T00~T19 A% B% A% B% 100% A% B% B%=400-A% T00 1. 0~999min enter 10 1.0min 2. T00 A enter B 3. T00 T1. 4. 2 3 4 46

1. edit 3. 4. enter Modify Method 05 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods 5. enter 1. edit 2. clear all methods Modify Method 05 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods 3. enter 4.enter 47

1. edit 2. 3. Modify Method 05 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods enter 4. 5. 6. enter 7. enter 8 1. edit Modify Method 05 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods 2. enter 48

3. 4. enter Delete Method 00 Clear ALL Methods 1 1). activate 2). 00~59 3). enter 4). run enter Mthd02 Step05 Link00 Flow 01.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 2 Modify Method 04 Copy Method 00 to 00 49

edit 1. edit Modify method 00 00 Copy method 00 to 00 00 00 Delete method 00 00 Clear all methods 00 0-59 60 enter 1. 1. edit 2. enter 3. 4. enter enter 2. 1. edit 2. 3. enter 4. 5. 6. enter 7. enter 50

4.8 3. 1. edit 2. LC-500HPLC 3. enter 4. enter 4. 1. edit 2. clear all methods 3. enter 4. enter 2. 1. 1. edit 2. edit 3. 1~59enter 2. 1. stop field 2. enter 0 1.0ml/min 10 10ml/min 51

4.8 3. 1. link field LC-500HPLC 2. enter 4. 1. flow rate 2. enter 5. 1. Slvnt A field 2. 3. Slvnt B field 4. * A B 5. 20 T00~T19 A% B% A% B% 100% A% B% B%=100-A% T00 2. 0~999min enter 10 1.0min 2. T00 A enter B 52

4.8 3. T00 T1. 4. 2 3 4 6. 1. activate 2. 00~59 3. enter 4. run enter R232 4.3 7725i 53

1 Mthd01 Step00 Link02 Flow 01.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 2 3 Mthd01 Step00 Link01 Flow 00.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 4 Mthd01 Step05 Link02 Flow 00.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 54

1 2 Modify Method 01 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 3 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A MEOH B H2O T00 004.0 A100 B000V 4 5 55

1 Modify Method 01 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods 2 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A IPA B H2O T00 004.0 A100 B000V 3 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T00 004.0 A100 B000V 4 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T00 000.0 A099 B001V 57

Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T01 085.0 A070 B030V Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T02 095.0 A030 B070V Mthd01 Flow Step05 01.000 Link02 ml/min Slvnt A H2O B MEOH T03 100.0 A098 B002V 9 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T04 110.0 A099 B001V 58

1 Modify Method 01 Copy Method 00 to 00 Delete Method 00 Clear ALL Methods 2 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A IPA B H2O T00 000.2 A100 B000V 3 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T00 000.2 A100 B000V 4 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T00 000.0 A090 B010V 59

Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T01 002.0 A090 B010V Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T02 070.0 A055 B045V Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T03 085.0 A055 B045V Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T04 086.0 A010 B090V 10 Mthd01 Step05 Link02 Flow 01.000 ml/min Slvnt A H2O B MEOH T04 093.0 A005 B095V 60

6 1. 1. flow 2. 0.001~10.00m/min 3. enter 4. run 5. enter 2. 1. 1/2 2. flow 5.00 3. run LED 4. purge, 10ml/mim 5. 6. stop 3.Pmax 62

1. Pmax 2. Psi 3. enter 4. Pmax 5. 90% Pmax Psi Psi 5400 90% 6000 2500 90% 2775 1200 90% 1300 9000 90% 10000 4. Pmin 1. Pmin 2. Psi 3. enter 4. 5. Pmin 63

1 2 1 2 3 64

1) 2) 3) 4) 5) 6 65

7) 8 9 10 11 12 13 66

1 2 3 67

1 68

2 69

5 70

1. 2. 3. 0.45 m 4. 71

WARNING!!! 1 1/16 SST ¼ ( 1). 1 () 72

. 1 () ( 9/64 ) ( 2) 2 73

5.2 (4) 8-32 X 1-1/2 2 500-1004 10ML T (4) 8-32 X 5/8 500-1250 74

(4) 8-32 X 1-1/2 1 75

(4)6-32 2 / 4 ½ ½ ½ Figure 4 Purge Valve Removal 500-3010 PEEK ½ 3 O 4 P-500 76

5 ul 400-0005 500-3111 10 ul 400-0010 500-3111 25 ul 400-0025 500-3111 50 ul 410-0050 500-3113 150 ul 410-0150 500-3113 250 ul 410-0250 500-3113 350 ul 420-0350 Not Required 500 ul 420-0500 Not Required ( ) 77

6 78

3 HPLC 0.5~1MPa () HPLC 0.45µm 02 1 2 3 4 10~15 peek HPLC 1 100% 3~5ml/min 2 3 595 / 1ml/min 30~60 5% 4 100% 1ml/min 2 81

() 30~40 HPLC HPLC 0.45µm 1 2 10-20 3 4 manual 5 manual purge start 10 ml/min 6 2000 injure on 7 file se-lect users nd methods new method protocol a. 2-5 b.c. loading-inject d. 8 start postrun 9 10 82

4 0.2ml/min 30 5 1 2 10 50 15 83

1 0.45 2 HPLC 045u 2ml/min [3] 84

7 85

LC-500A EECO 10 5~28DCV 2 1 LC-500A 10 6 / 11 5v 5v 28VDV T00 T00 T00T01 V T00 0.000 A000 B000 Venter V 1 00 2 00 3 00 4 00 1 V1 20 T00~T19 86

T00 1 001.0 1min 000.5=0.5min 30s 87

SPD-500 / 1...3 1.1...3 1.2...3 1.3 SPD-500 /...4 2...7 3...7 3.2...8 4...9 4.1...9 4.2...11 4.3...13 4.3.1...14 4.3.2...15 4.3.3...16 4.3.4...17 4.3.5 GLP...17 5...19 5.1 1...19 5.2 2...20 5.3 3...22 5.4 4...23 5.5 5...24 5.6 6...25 6...26 7...29 2

SPD-500 / SPD-500 / MODEL 501/500 SPD-500 1. SPD-500 / SPD-500 log I1 A = ε L log I 0 I 1 I 0 L 3

SPD-500 / 1) : 2) 3) 4) SPD-500 / 2. SPD-500 1- ( ) 2-3- 4-5- 6-7- 8-9- 123 56 4

SPD-500 / 7 1 2 3 4 5 6 7 8LCD(12265B) 9LCD(12265B) 10 11 () 12 13 14 15() 16 17 18DC-DC 5

SPD-500 / RS232 SPD-500 RS232. +5V,2A(15V,0.5A) 14 6

SPD-500 / 3.1 SPD-500 a. b. c. d. e. f. 100cm g. A) 4~35, B) C) D) E) 45~85% 7

SPD-500 / 3.2 SPD-500 1. SPD-500 / 2. 3. 4. RS-232 (SPD-500 ) 5. 4 1 8

SPD-500 / 9

SPD-500 / 4. SPD-500 4.2 Menu. 1530 1 2 3 4.5 SPD-500 : 1(Model:16265B),. 2. 3. 4. 10

SPD-500 / SPD-500 4 16 SPD-500 6 GLP-GLP- 09 A/Z 2 01 4.3.1 11

SPD-500 / Fig. 12

SPD-500 /.6 3 DH DH 01 Enter. 2).7 / ON OFF 01 Enter 3) WLnm Enter 13

SPD-500 / 190700nm 1nm.8 1) RANGE(Au) 09 1Au/mV 2000mV 1Au 10.00 5.000 2.000 1.000 0.500 0.200 0.100 0.050 15 14 13 12 11 10 9 8 0.020 0.010 0.005 0.002 0.001 00005 0.0002 0.0001 7 6 5 4 3 2 1 0 1: RANGE TIME CON. 0.1,0.2,0.5,1,2,5 10 1 2 14

SPD-500 /.9 1) 1.10 15

SPD-500 / mv 2.11 S: R: GLP GLP (CMC) (System time)(lamp time) 16

SPD-500 /.12 GLP 17

SPD-500 / 1 N2000, 254nm 2s 1AuFS 012345 Enter N2000 C holmium d)~e) C blank, C holmium C blank, 3 1 2 3 18

SPD-500 / 2 1 3.13 361nm,446nm 536nm. 4.2.3 2 19

SPD-500 /.14 200ml 0.13% A 200ml B 3ml/min : 254nm : 2AuFS : 2s 30 B 2 A/Z A read_data (mau) B A 112mAu read _ data gain _ value = old _ gain _ value 112 : gain_value Old_gain_value 0.1 The read_data B A :mau 4.2.3 d) 2 20

SPD-500 / 3.15.16 15 3 3 4 GLP () 21

SPD-500 / 4.2.1 4.16 15 2 2 4 GLP () 4.2.1 22

SPD-500 / 5.17 3 2.19 1ml/min 80:20, 15 23

SPD-500 / SAM/REF 5 6 100ml 1Mol 3ml/min 15 ph 15 24

SPD-500 / ( 1. ) () +5V () E 2 ROM, CPLD (12265B) 2. 3. Key board Error 4. RAM E 2 PROM (AT28C64 AT28C64 Check the connector & cable 82C79 from main board to keyboard And then restart the instrument If the trouble still occur, please contact the local representation of the factory RAM 5. Deuterium lamp D D does not start 6. 7. H H 25

SPD-500 / OVERFLOW 8. SAM/REF 5,6 MAX203 9. 2 SAM 1 2s 1 2 10. 26

SPD-500 / 27

SPD-500 / 1

1 LC-500 2 LC-500 3 4 4.1 2 LC-500A Rheodyne 7725i SPD-500 -N2000 4.2 4.2.1 0.45m 20min 4.2.2 4.2.3 0.45m 4.2.4 4.3 N2000 4.4 4.4.1 [ ] [ ] 4.4.2 [Flow] 1ml/min[Enter] 4.4.3 4.5.1 4.5.2 180 4.5.3 [purge]run 10.0ml/min 3min STOP 4.5.4 4.5.5 4.5.6 5ml/min[RUN] 3min [STOP] 4.6 4.6.1 N2000 [] 1

4.6.2 4.6.2.1 [Run]RUN 6 4.6.2.2 4.6.2.3 15psi 4.5.6 4.6.2.4 <0.1mV/min<0.01mV 4.6.3 4.4.4.1 A [edit] 4.4.4.2 [Enter]FLOW TOO B [Enter][Enter] 4.4.4.3 edit 2. edit Midify method 00 00 copy method 00 to 00 00 00 Delete method 00 00 Clear all methods 01 0-59 60 enter 1. 1. edit 2. enter 3. 4. enter enter 2. 1. edit 2. 3. enter 4. 5. 2

6. enter 7. enter 3. 1. edit 2. 3. enter 4. enter 4. 1. edit 2. clear all methods 3. enter 4. enter 2. 1. 1. edit 2. edit 3. 1~59enter 2. 1. stop field 2. enter 0 1.0ml/min 10 10ml/min 3. 1. link field 2. enter 4. 1. flow rate 2. enter 3

5. 1. solvent A field 2. 3. solven B field 4. *A B 5. 20 T00~T19 A% B%A% B% 100% A% B%B%=400-A% T00 3. 0~999minenter 10 1.0min 2. T00 A enter B 3. T00 T1. 4. 234 6. 1. activate 2. 00~59 3. enter 4. run enter R232 4.5 4.5.1 A/B 4.5.2 A/B 180 4.5.3 A/B [purge]pump 10.0ml/min 3min 4.5.4 4

4.5.5 4.5.6 5ml/min[pump] 3min [pump] 4.6.3.1 4.6.3.2 1~2 A [Activte] [run] RUN OFF 4.7 4.7.1 [zero] [] 4.7.2 5

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