32 1 2013 2 Journal of Chinese Electron Microscopy Society Vol. 32 No. 1 2013-02 1000-6281 2013 01-0054-08 1 1* 2 1 1 1. 2. 710062 AFM SEM WPOJ-DS UPOJ-DS WPOJ-DS β- UPOJ-DS AFM WPOJ-DS UPOJ-DS SEM UPOJ-DS WPOJ-DS S567. 23 + 2 TG115. 21 + 5. 3 TG115. 21 + 5. 7 A doi 10. 3969 / j. 1000-6281. 2013. 01. 010 1 ~ 3 4 1. 1 1 DEAE-cellulose52 Whatman Sephacryl S-300 Pharmacia 95% 5 ~ 8 SPM-9500J3 TU-1810 - Avatar360E. S. P. FTIR RE-52AA FD-1A 9 10 Quanta 200 FEI WPOJ-DS UPOJ-DS 1. 2 1. 2. 1 60 4 95% 3 1 80 g WPOJ-DS UPOJ-DS 1 10 80 2 h 2 80 g 1 10 2012-08 - 04 2012-11 - 05 No. 10874108 No. SJ08A16. 1983 -. E-mail wxm19830427@ 163. com *. E-mail sunrunguang@ snnu. edu. cn
1 55 15 s 15 contact s 90 400 W Si 3 N 4 200 μm 0. 12 N / m 4 AFM 95% AFM WPOJ UPOJ 1. 2. 4 - WPOJ UPOJ 260 ~ 280 nm WPOJ-DS UPOJ-DS 1 cm 1 cm DEAE-cellulose52 20 kv SEM 6 s / 15 min / 2 WPOJ-DS UPOJ-DS - 490 nm SEM DEAE-cellulose52 2 WPOJ-D UPOJ-D WPOJ-D UPOJ-D 2. 1 Sephacryl S-300 1 WPOJ-DS UPOJ-DS 5 s / 15 min - WPOJ- DS UPOJ-DS 3 361 cm - 1 3 334 cm - 1 O H 1. 2. 2 2 936 cm - 1 2 935 cm - 1 1 mg WPOJ-DS UPOJ- DS 100 mg KBr cm - 1 1 658 cm - 1 N H 4 000 ~ 400 cm - 1 1 200 ~ 1 400 cm - 1 C 1. 2. 3 H 1 000 ~ 1 200 cm - 1 1 mg WPOJ-DS UPOJ-DS 50 μg / ml 820 cm - 1 822 4 h cm - 1 α- 400 ~ 600 cm - 1 25 μg / ml 10 μg / ml 5 μg / ml 1 10 μg / ml CH 2 C H 1 649 C O 934 cm - 1 935 cm - 1 5 μl WPOJ-DS 872 cm - 1 UPOJ-DS SPM WPOJ-DS β- 11 9500J3 1 Fig. 1 a WPOJ-DS b UPOJ-DS IR spectrum of WPOJ-DS a and UPOJ-DS b.
56 J. Chin. Electr. Microsc. Soc. 32 2. 2 90 nm WPOJ-DS UPOJ-DS 1 nm 1 AFM UPOJ-DS 2 25μg / ml AFM WPOJ-DS 30 ~ 70 nm 8 ~ 20 nm UPOJ-DS 3 10 μg / ml WPOJ- Fig. 2 2 25 μg / ml WPOJ-DS a ~ c UPOJ-DS a' ~ c' AFM a ~ c Bar = 5 μm 1 μm 200 nm a' ~ c' Bar = 5 μm 5 μm 200 nm AFM images of WPOJ-DS a - c and UPOJ-DS a' - c' on the concentration of 25 μg / ml. DS 1. 3 nm ~ nm UPOJ-DS 80 nm 10 nm β- 4 5 μg / m WPOJ-DS UPOJ-DS 2 ~ 4 AFM AFM WPOJ-DS 50 nm 1 mm 2. 3 5a 5b WPOJ-DS UPOJ- DS 50 nm WPOJ-DS nm 150 5a' 5b' UPOJ-DS
1 57 Fig. 3 3 10 μg / ml WPOJ-DS a ~ c UPOJ-DS a' ~ c' AFM a ~ c Bar = 5 μm 1 μm 500 nm a' ~ c' Bar = 5 μm 1 μm 200 nm AFM images of WPOJ-DS a - c and UPOJ-DS a' - c' on the concentration of 10 μg / ml. Fig. 4 4 5 μg / ml WPOJ-DS a ~ c UPOJ-DS a' ~ c' AFM a ~ c Bar = 5 μm 2 μm 500 nm a' ~ c' Bar = 5 μm 1 μm 200 nm AFM images of WPOJ-DS a - c and UPOJ-DS a' - c' on the concentration of 5 μg / ml.
58 J. Chin. Electr. Microsc. Soc. 32 UPOJ-DS WPOJ-DS WPOJ-DS UPOJ-DS 6 a ~ d WPOJ-DS 5 WPOJ-DS a b UPOJ-DS a' b' SEM a b a' b' Bar = 20μm Fig. 5 SEM images of mica samples of WPOJ-DS a b and UPOJ-DS a' b'. UPOJ-DS WPOJ-DS WPOJ-DS 6 a' ~ d' UPOJ-DS β- UPOJ-DS WPOJ-DS UPOJ-DS 3 5 6 β-
1 59 Fig. 6 6 WPOJ-DS a ~ d UPOJ-DS a' ~ d' SEM a ~ d Bar = 200 50 20 10 μm a' ~ d' Bar = 200 50 20 10 μm SEM images of powder samples of WPOJ-DS a - d and UPOJ-DS a' - d'.
60 J. Chin. Electr. Microsc. Soc. 32 japonicus J. Carbohydrate Polymers 2011 83 2 749-754. 4 Ying Zhi Han Xiaoxiang Li Jianrong. Ultrasoundassisted extraction of polysaccharides from mulberry β- leaves J. Food Chemistry 2011 127 1273-1279. 5. J. 1999 18 1 103-105. 6. J. 2006 64 24 2467-2472. 7 Camesano T A Wilkinson K J. Single molecule study of xanthan. conformation using atomic force microscopy 1 Li Y N Zhu D N Qi J et al. Characterization of homoisoflavonoids in different cultivation regions of Ophiopogon japonicus and related antioxidant activity J. Journal of Pharmaceutical and Biomedical Analysis 2010 52 5 757-762. 2 Xiong Shuangli Li Anlin Huang Ni et al. Antioxidant and immunoregulatory a ctivity of different polysaccharide fractions from tuber of ophiopogon japonicus J. Carbohydrate Polymers 2011 86 3 1273-1280. 3 Chen X M Jin J Tang J et al. Extraction purification characterization and hypoglycemic activity of a polysaccharide isolated from the root of ophiopogon J. Biomacromolecules 2001 2 1184-1191. 8 Ding Xiang Feng Su Cao Mei et al. Structure characterization of polysaccharide isolated from the fruiting bodies of Tricholoma matsutake J. Carbohydrate Polymers 2010 81 942-947. 9. J. 1990 1 60-66. 10. Bel7402 J. 2003 6 467. 11 Ding Xiang Tang Jie Cao Mei et al. Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake J. International Journal of Biological Macromolecules 2010 47 2 271-275.
1 61 Comparative study on the structure and aggregation behavior of ophiopogon japonicus polysaccharides extracted by two methods WANG Xiao-mei 1 SUN Run-guang 1* ZHANG Jing 2 HAO Chang-chun 1 ZHANG Li-ni 1 1. College of Physics and Information Technology 2. College of Food Engineering and Nutritional Science Shaanxi Normal University Xi'an Shaanxi 710062 China Abstract In this paper different extraction methods including hot water and ultrasound were used to extract polysaccharides in ophiopogon japonicus. The functional group distribution and surface morphology of polysaccharides extracted by the two methods were studied by FT-IR spectroscopy atomic force microscopy AFM and scanning electron microscopy SEM. The results showed that the difference of functional group between ophiopogon japonicus polysaccharides extracted by hot water WPOJ-DS and ultrasound UPOJ-DS was obvious. β-glucopyranoside was detected in WPOJ-DS using infrared spectroscopy but not in UPOJ-DS. The results of AFM showed that the dispersion of UPOJ-DS was significantly higher than that of WPOJ-DS. Single molecule chain and helical structure were observed in UPOJ-DS by AFM indicating that intermolecular and intramolecular hydrogen bonds of polysaccharides were affected by ultrasound. The results of SEM showed that less of bigger schistose structures were observed in UPOJ-DS compared to WPOJ-DS and a lot of rod-shaped and small globular aggregates were observed in UPOJ-DS indicating that supersonic treatment created degradation on ophiopogon japonicus polysaccharides. Therefore ultrasonic extraction has an effect on molecular structure and aggregation behavior of ophiopogon japonicus polysaccharides. Keywords ophiopogon japonicus polysaccharides ultrasound atomic force microscope AFM scanning electron microscope SEM * Corresponding author