7 12 Vol. 7 No. 12 2016 12 Journal of Food Safety and Quality Dec., 2016 陈笑笑 1, 赵芸 2, 柳爱春 2, 陈飞东 2, 胡叶军 1*, 叶聪莹 (1., 310051; 2., 310024) 1 摘要 :,,,,,,,,, 关键词 : ; ; ; Research progressof detection methods for streptomycin residue in food CHEN Xiao-Xiao 1, ZHAO Yun 2, LIU Ai-Chun 2, CHEN Fei-Dong 2, HU Ye-Jun 1*, YE Cong-Ying 1 (1. Hangzhou Nankai Biotech Co., Ltd., Hangzhou 310051, China; 2. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China) ABSTRACT: Streptomycin is a kind of broad-spectrum aminoglycoside antibiotics. Because of its good antibacterial effect and low price, it is often used as a feed additive, treatment agent, fruit and vegetable disease control agent which are usually used in animal husbandry, apiculture, aquaculture and agriculture. The streptomycin residue in animals and plants increases by the extensive use of streptomycin in agriculture and animal husbandry. Then the streptomycin residue enters human body by the food chain and causes great harm to human health. Many countries have paid great attention to streptomycin residue in food, and set up streptomycin residue limit standards. Considering food safety, it is of great significance to establish a method for the detection of streptomycin in food. This paper provided a comprehensive overview of the research status of streptomycin detection technologies in food, and focused on the microbiological assay, immunoassay, chromatography, and spectrophotometric method for the detection of streptomycin. The advantages and disadvantages of these detection methods were also analyzed as well as the development trend. KEY WORDS: streptomycin; residue; food; detection 1 引言 (streptomycin, STR) 1944 Selman Abraham Waksman,, [1], 基金项目 : 2013 (20131812A05) Fund: Supported by the Hangzhou Major Scientific and Technological Innovation Project in 2013 (20131812A05) * 通讯作者 :,, E-mail: hyj664@163.com *Corresponding author: HU Ye-Jun, Assistant Engineer, Hangzhou Nankai Biotech Co., Ltd., Hangzhou 310051, China. E-mail: hyj664@ 163.com
4816 7, [2], [3],, [3], [4],,, [5] ;, [6] ;, [7], [8] ;, [9] (EC) No 470/2009, 1000 μg/kg, 200 μg/kg, 500 μg/kg [10], 2000 μg/kg, 500 μg/kg [11] 2002, 200 μg/kg, 600 μg/kg, 1000 μg/kg [12],,,, 2 微生物法, [13] Swab Test on Premises(STOP) [14] Fast Antibiotic Test(FAST) [15] TTC [16] [17] [18],, 0.1 μg/ml, 200 μg/kg [19] TTC,, 660 μg/kg,, [20], 80 μg/kg,,,,,, [19,21,22], 3 免疫分析法, 3.1 酶联免疫分析法 (enzyme-linked immunosorbent assay, ELISA),, [23] [24] ELISA 2.7 ng/ml 2.8 ng/ml, [25] ELISA, 4 μg/kg, 0.1 μg/kg, [25] ELISA, 0.5~128 μg/l, 30 μg/kg ELISA [26],, 3.2 放射免疫测定法 (radioimmunoassay, RIA) [27], [28] Charm Ⅱ, 25~500 μg/l,, 200 μg/kg [29] Charm Ⅱ, 10 μg/kg,,,, [29], [30], 3.3 胶体金免疫层析技术 (gold immunochromatography assay, GICA) 3 [31] [32], 10 ng/ml, 40 ng/ml [33],, 8 μg/l, 100 μg/l,,,,, [32-34],,
12, : 4817 4 色谱分析法,,, [35] - 4.1 液相色谱法,, (high performance liquid chromatography, HPLC),,, [36] [37] - [36] [37], -, 0.005 mg/kg [38],, 10 μg/kg,,,, [36],,,, 4.2 色谱 - 质谱联用法 (hydrophilic interaction chromatography, HILIC), 玥 [39],, 0.5 μg/kg 1 μg/kg [40],, - (UPLC-MS/MS), 10 μg/l 5 化学发光法 (chemiluminescence, CL),, [41] [42] 100 g/l, C 18, ph, 50 μg/kg,, [43], 6 分光光度法, [44], (o-phthalaldehyde, OPA),,, -NH 2, 1,2- -4-,, [45],,,,, [46], 7 共振瑞利散射法 (resonance Rayleigh scattering, RRS),, [47,48], [49] Triton X-100,, RRS,,,, [50] 8 存在的问题与发展前景,, -,,,,,
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