中国科学院鹤山丘陵综合开放试验站

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1 中 国 科 学 院 鹤 山 丘 陵 综 合 开 放 试 验 站 论 文 汇 编 (2001 年 ) Scientific article collection of Heshan Hilly land Interdisciplinary Experimental Station, CAS (Yr. 2001)

2 目 录 CPPU 提 高 柚 树 苗 抗 旱 性 的 研 究 (1) Meta 分 析 中 几 种 常 用 效 应 值 的 介 绍 (7) UV-B 辐 射 对 香 蕉 光 合 作 用 和 不 同 氮 源 利 用 的 影 响 (11) 桉 树 人 工 林 地 土 壤 肥 力 灰 色 关 联 分 析 (19) 变 化 的 地 球 面 临 挑 战 全 球 变 化 开 放 科 学 大 会 简 介 (22) 不 同 光 强 下 生 长 的 厚 叶 木 莲 (Magnolietia pachyphylla) 光 合 作 用 光 响 应 的 变 化 (23) 长 期 气 温 波 动 对 鼎 湖 山 马 尾 松 种 群 生 产 力 的 影 响 (29) 大 气 CO 2 浓 度 升 高 对 香 蕉 光 合 作 用 及 光 合 碳 循 环 过 程 中 叶 氮 分 配 的 影 响 (34) 广 东 南 澳 岛 次 生 林 的 群 落 结 构 分 析 (40) 广 东 南 澳 岛 植 被 恢 复 过 程 中 的 群 落 动 态 研 究 (47) 贵 州 茂 兰 喀 斯 特 的 自 然 森 林 景 观 (55) 海 岛 退 化 生 态 系 统 的 恢 复 (59) 海 南 野 生 香 根 草 资 源 调 查 报 告 (64) 华 南 不 同 人 工 林 土 壤 铵 吸 附 特 征 及 其 吸 附 动 力 学 研 究 (68) 化 感 作 用 的 研 究 意 义 及 发 展 前 景 (75) 基 于 GIS 和 RS 的 广 东 陆 地 植 被 生 产 力 及 其 时 空 格 局 (82) 基 于 NOAA_AVHRRNDVI 和 GIS 的 广 东 植 被 光 能 利 用 率 及 其 时 空 格 局 (89) 几 种 数 量 综 述 方 法 的 介 绍 与 比 较 (95) 跨 越 边 界 的 生 态 恢 复 第 十 三 届 国 际 恢 复 生 态 学 大 会 综 述 (100) 两 种 山 黄 麻 属 植 物 在 近 一 世 纪 里 气 孔 密 度 和 潜 在 水 分 利 用 率 的 变 化 (101) 林 窗 与 生 物 多 样 性 维 持 (106) 马 占 相 思 (Acacia mangium) 与 湿 地 松 (Pinus elliotii) 人 工 林 枯 落 物 层 的 水 文 生 态 功 能 (112) 马 占 相 思 人 工 林 温 湿 效 应 的 时 空 动 态 (117) 梅 州 沙 田 柚 平 衡 施 肥 研 究 (124) 南 澳 岛 植 被 恢 复 过 程 中 不 同 阶 段 土 壤 的 变 化 (130) 南 亚 热 带 丘 陵 区 龙 眼 和 象 草 的 叶 片 气 孔 气 体 交 换 的 研 究 (135) 全 球 变 化 背 景 下 大 气 CO 2 浓 度 升 高 与 森 林 群 落 结 构 和 功 能 的 变 化 (141) 三 个 不 同 海 拔 梯 度 马 尾 松 种 群 的 遗 传 多 样 性 及 其 与 生 态 因 子 的 相 关 性 (149) 沙 田 柚 施 用 活 化 处 理 磷 肥 的 效 果 研 究 (156) 沙 田 柚 树 体 营 养 特 性 研 究 (163) 沙 田 柚 系 列 遗 传 关 系 的 RAPD 标 记 研 究 (170) 沙 田 柚 优 质 高 产 的 土 壤 改 良 与 施 肥 及 修 剪 控 梢 技 术 (175) 韶 关 市 生 态 旅 游 资 源 现 状 分 析 及 其 开 发 构 想 (181) 生 物 入 侵 及 其 影 响 (186) 树 木 年 轮 δ~13c 含 量 幼 龄 效 应 的 定 量 化 探 讨 (191) 水 分 胁 迫 对 长 期 UV_B 辐 射 下 柚 树 苗 生 理 特 性 的 影 响 (195) 通 过 树 木 年 轮 δ~13c 重 建 大 气 CO 2 碳 同 位 素 比 δa 的 可 靠 性 探 讨 (201) 退 化 森 林 生 态 系 统 恢 复 过 程 的 碳 同 位 素 示 踪 (206) 微 甘 菊 花 挥 发 油 成 分 分 析 (215) 薇 甘 菊 的 综 合 开 发 与 利 用 前 景 (217) 我 国 热 带 亚 热 带 几 种 人 工 林 体 内 营 养 结 构 特 征 (221) I

3 香 根 草 和 百 喜 草 对 铅 锌 尾 矿 重 金 属 的 抗 性 与 吸 收 差 异 研 究 (226) 应 用 正 交 设 计 方 法 优 化 香 蕉 外 植 体 直 接 出 芽 的 条 件 (235) 影 响 季 风 常 绿 阔 叶 林 幼 苗 定 居 的 主 要 因 素 (241) 柚 类 品 种 遗 传 相 互 关 系 的 RAPD 标 记 研 究 (247) 柚 类 种 质 资 源 分 类 鉴 定 初 步 研 究 (254) 柚 类 种 质 资 源 研 究 与 保 护 概 况 (261) 柚 品 种 的 等 位 酶 变 异 研 究 (267) 柚 树 (Citrus grandis) 叶 片 光 合 作 用 对 补 增 UV-B 辐 射 的 响 应 (275) 有 机 活 化 剂 对 磷 矿 粉 活 化 效 果 研 究 (280) 在 高 CO 2 浓 度 下 四 种 亚 热 带 幼 树 光 合 作 用 对 水 分 胁 迫 的 响 应 (285) 增 强 UV-B 辐 射 对 柚 树 苗 生 长 和 生 理 特 性 效 应 研 究 (294) 植 物 分 子 生 态 学 进 展 (Ⅰ) 遗 传 结 构 和 杂 交 ( 英 文 ) (302) 植 物 群 落 波 动 的 类 型 与 机 理 ( 综 述 ) (310) 植 物 群 落 的 演 替 理 论 (317) 植 物 生 理 生 态 指 标 对 大 气 CO 2 浓 度 倍 增 响 应 的 整 合 分 析 (326) 中 国 东 部 陆 地 农 业 生 态 系 统 与 全 球 变 化 相 互 作 用 机 理 研 究 取 得 重 要 进 展 (335) 种 种 的 多 样 性 及 退 化 生 态 系 统 功 能 的 恢 复 和 维 持 研 究 (338) II

4 2001, 18 (1) :90 95 Chinese Bulletin of Botany CPPU ( ), RWC W P n, MDA SOD,CAT,APX AsA GSH, CPPU RWC P n AsA GSH, SOD APX, MDA,,CPPU CPPU,, Enhancement of Drought Resistance in Pomelo Seedlings by CPPU NIE Lei LIU Hong- Xian PENG Shao-Lin ( South China Institute of Botany, The Chinese Academy of Sciences, Guangzhou ) Abstract Under water stress, leaf RWC, W, P n,soluble protein and chlorophyll contents in pome2 lo seedlings were decreased, the contents of MDA and free proline, SOD activity were increased, CAT activity increased at first, then decreased. And activity of APX, the contents of AsA and GSH were declined too. The activated oxygen decomposing capacity in the drought- resistant variety was higher than that in the drought- sensitive one. CPPU treatment increased leaf RWC, P n, chloro2 phyll,free proline,asa, GSH contents, SOD,APX activities, and decreased the accumulation of MDA, therefore the drought resistance of pomelo seedlings was improved. These results indicated that CPPU treatment increased protective capacity against membrane lipid peroxidation, therefore re2 sulted in the increase of drought resistance of pomelo seedlings. Key words CPPU, Pomelo seedlings, Drought resistance CPPU, N- -N - (2-4- ), 1000 CPPU, (, 1993) (1997) CPPU KT 6-BA,,, CPPU,, CPPU, (No ) :,,1973,,,1933,,,, 70 : : :

5 1 : CPPU , ( Citrus grandis Osbeck. ),, 9, 6, 3 1d, 20 mg l - 1 CPPU,, CPPU ( 8 ) ( 14 ),, Hsiao (1973) :RWC 10 % 20 %,RWC 20 % 8 :00 9 :00, ( W ) (RWC) (1980) (MDA) Raeri (1993) Bradford (1976), Arnon (1949) ( P n ), nm, 3 (SOD) Giannopolitics Ries (1977), NBT 50 % ( CAT) Klapheck (1990) (APX) ( GSH) (1990) (AsA) Tanaka (1985) CPPU MDA, CPPU,5d MDA,5 50 mg L - 1 CPPU, MDA, 20 mg L - 1 CPPU, CPPU, 20 mg L - 1 CPPU 1,,2 RWC, RWC,,, MDA,, RWC MDA [y = x,y MDA,r = ;y = x,y MDA, r = ] RWC CPPU,,MDA CPPU CPPU

6 92 18 Cultivars and treatment 1 CPPU MDA Table 1 Effects of CPPU on leaf water potential, relative water content and MDA content in pomelo seedlings under water stress Suanyou Normal irrigation Moderate stress Control CPPU Severe stress Control CPPU Shatianyou Normal irrigation Moderate Stress Control CPPU Severe stress Control W (MPa) RWC( %) MDA( mol g - 1 FW) a a e b b c b ab d cd d a c c b a a d b b c b b c d d a CPPU c c b 3, 5 % Mean within columns followed by the same letter are not sig2 nificantly different at 5 % level. 212 CPPU,,,CPPU CPPU, 7. 9 %,10. 1 %,5. 4 % 4. 3 %,7. 1 %,5. 1 %, 7. 3 %,8. 0 %,5. 7 % 6. 5 %,7. 5 %,8. 6 %, %, % %,56. 1 % CPPU ( 2) 213 CPPU, SOD, ( 3) CAT, CPPU CAT

7 1 : CPPU 93 APX AsA GSH,, SOD APX AsA GSH CPPU SOD APX AsA GSH, 2 CPPU Table 2 Effects of CPPU on leaf P n and the contents of chlorophyll,soluble proteins, free proline in pomelo seedlings under water stress ( P n ) Cultivars and treatment ( mol m - 2 s - 1 ) Suanyou (mg g - 1 FW) a a a Normal irrigation Moderate stress Control CPPU ( mol g - 1 DW) ( g g - 1 FW) b bc b c b b b c Severe stress Control c d cd b CPPU Shatianyou b bc c a a a a Normal irrigation Moderate stress Control CPPU b bc c c b b b ab Severe stress Control d d d ab CPPU c bc c a 3, 5 % Mean within columns followed by the same letter are not sig2 nificantly different at 5 % level. 3,, ABA (Dhindsa, 1982 ; Leshem,1981), (Smirnoff,1993) (1991),,CPPU,,

8 94 18 (MDA) MDA CPPU MDA,, 3 CPPU Table 3 Effects of CPPU on leaf protective systems against membrane lipid peroxidation of pomelo seedlings under water stress SOD CAT APX Cultivars and (Unit mg treatment - 1 protein) ( mol mg - 1 min - 1 )( mol mg - 1 min - 1 ) Suanyou AsA ( mol g - 1 DW) GSH ( mol g - 1 DW) d c a a a Normal irrigation Moderate stress c a c bc c Control CPPU b ab b b ab Severe stress b d d d d Control CPPU Shatianyou a d c c c d c a a a Normal irrigation Moderate stress bc a bc bc bc Control CPPU b ab b b b Severe stress ab d d d de Control CPPU a cd c c d 3, 5 % Mean within columns followed by the same letter are not sig2 nificantly different at 5 % level.,, Pro (Veeranjaneyulu,1989),CPPU SOD,CAT,APX AsA,GSH SOD,CPPU SOD,CAT APX AsA GSH H 2 O 2,CAT,,CPPU CAT, CPPU CAT, (,1998),

9 1 : CPPU 95 APX AsA GSH, CPPU APX AsA GSH, CPPU H 2 O 2 CPPU, :,1 2,,,, PU-30.,23 : ,1993. CPPU.,29 : ,,,,1998..,40 : ,,,1991..,17 :80 84,,1990..,16 :37 42 Arnon D,1949. Copper enzymes in isolated chloroplasts :Polyphenoloxidase in Beta vulgris. Plant Physiol,24 :1 15 Bradford M,1976. A rapid and sensitive method for the quantitation of microgram quantities of protein ultilizing the of protein- dye binding. Anal Biochem, 72 : Dhindsa R S, Dhindsa P P, Reid M,1982. Leaf senescence and lipid peroxidation : Effects of some phytohormones,and scavengers of free radicals and singlet oxygen. Physiol Plant, 56 : Giannopolitics C N, Ries S K, Superoxide dismutase.. Occurrence in higher plants. Plant Physiol, 59 : Hsiao T C, Plant responses to water stress. Ann Rev Plant Physiol,24 : Klapheck S, Zimmer I,Cosse H,1990. Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxi2 dase. Plant Cell Physiol,11 ; Leshem Y Y, Wurzburger J, Grossman S, Cytokinin interaction with free radical metabolism and senescence : Effects of endoge2 nous lipoxygenase and purine oxidation. Physiol Plant,53 :9 12 Raeri A, Lencioni L, Schenone G, Glutathione-ascorbic acid cycle in pumplein plants grown under polluted air in open- up chambers. J Plant Physiol,142 : Smirnoff N, The role of active oxygen in the responses of plants to water deficit and desiccation. New Phytol,125 :27 58 Tanaka K, Suda Y, Kondo N, Nakano Y, Ozone tolerance and the ascorbate- dependent hydrogen peroxide decomposing system in chloroplasts. Plant Cell Physiol, 26 : Veeranjaneyuiu K, Ranjita K B D, Kar M, Proline metabolism during water stress in mulberry. J Exp Bot,214 : , 20 90, 3, :, : : ; ; ; ; ; 2001,,,, : 0137,, ( ), 3. 70, , : ISSN , :CN / Q, :M320, :2-506,,, 2. 00, , : (010)

10 20 1, Ecologic Science Vol120 No11, 2 Jun Meta Ξ, (, ) : Meta,, 3 : ; Meta : Q-332 : A : (2001) 01, Meta,, 20 90, [1 Meta ], [2 ] Meta Meta Meta, Meta, ( ),,,, 3 : ; 2 2 ; t- F- 3 1,,,,, 111 Glass = ( X e - X c ) / S c (1) V = N c + N e 2 + N c N e 2 ( N c - 1), X e, X c, S c, N e, N c [3 Glass ],, (2) Ξ : ( ) ; ( K2951- B1-110) ; (980952) ; (003031) : ; : ( ),,.

11 82 20,, Meta, 112 Hedges g [4 Hedges Glass ], S, Hedges g S = V g g = ( X e - X c ) / S (3) ( N e - 1) ( S e ) 2 + ( N c - 1) ( S c ) 2 N e + N c - 2 = N c + N e g 2 + N c N e 2 ( N c + N e - 2), S e, 113 Hedges d Hedges g Glass,, [5, Hedges 1985 J ], Hedges d J = 1 - V d d = ( X e - X c ) J (6) S 3 4 ( N c + N e - 2) - 1 = N c + N e d 2 + N c N e 2 ( N c + N e ) 5, d 114 (response ratio, ln R) Hedges 1999, (ln R) [6 ], : ln R = ln X e / X c = ln X e - ln X c (9) V ln R = S e 2 N e X e 2 + (4) (5) (7) (8) S 2 c 2 (10) N c X c ln R Hedges d,, 4 - +, 0, ; 0, ; , 2 2 ( 1),

12 1, 2 : Meta A B A + B C D C + D N t = A + C N c = B + D N = A + B + C + D P t = A/ N t (11) P c = B/ N c (12) P t P c, (rate difference, RD) [7 ], : RD = P t - P c (13) V RD = P t (1 - P t ) N t + P c (1 - P c ) N c (14), P t P c, P t P c 0, 1, RD, 015 [8, RD, ] 212 (relative rate, RR) [7 ], RD RR = P t / P c (15) V RR = (1 - P t) N t P t + (1 - P c) N c P c (16) RR = 0, ; 0 < RR < 1, ; RR > 1, 3 Meta, 2 ( r), t F 2 t 2 F r = r = r =, F + df t 2 + df 1) ; F - t - 2, t-, df = N c + N e - 2; [9 ], F-, df = N c + N e - 1 1) 2 N e + N c [10 Fisher z- ]

13 84 20 z = 1 2 ln 1 + r 1 - r (17) V z = 1 ( N e + N c ) - 3 z - +,,, 0, Hedges d ln R, Meta,,, CO 2 Meta, ln R d, ln R : [1 ],. Meta : [J ].,1999,18(6) : [2 ],. Meta [J ].,1999,19(4) : [3 ] GLASS G V. Primary, secondary and meta-analysis of research[j ]. Educational Researcher,1976,5 :3-8. [4 ] HEDGES L V. Distribution theory for Glass s estimator of effect size and related estimators[j ]. J Educational Statistics,1981, 6 : [5 ] HEDGES L V, OLKIN I. Statistical methods for Meta-analysis[M]. New York : Academic Press,1985. [6 ] ROSENBERG M S, ADAMS D C, GUREVITCH J C. MetaWin : statistical software for Meta-analysis with resam2 pling tests[m]. Verion1. 0. Sinauer Associates, Sunderland Massachusetts [7 ] NORMAND S L T. Meta - analysis :formulating, evaluating, combining, and reporting[j ]. Statistics in Medicine, 1999, 18 : [8 ] FRIEDMAN H. Magnitude of experimental effect and a table for its rapid estimation[j ]. Psychological Bulletin, 1968, 70 : [9 ] FLEISS J L. Measures of effect size for categorical data[a]. In :COOPER H,HEDGES L V, eds. The handbook of research dynthesis[m]. New York :Russell Sage Foundation, [10 ] FISHER R A. Statistical methods for research worders[m]. 2nd ed.london : Olover and Boyd,1932. (18) Introduction to Connonly Used Effect Sizes in Meta-analysis ZHENG Feng- ying, PENG Shao- lin (South China Institute of Botany, Academia Sinica, Guangzhou , China) Abstract : Effect size is the statistic in quantitative Meta- analysis, the calculation of which depends on primarily the data available from the original studies. Because the data from original studies are pre2 sented in different forms, methods for calculating effect sizes were developed for these different types of primary data. These effect sizes suited to three different types of data were introduced and compared. Keywords : effect size ; Meta- analysis

14 2001, 25 (3) A cta Phytoecolog ica S in ica UV-B Ξ (, ) (, ) NO - 3 2N N H + 4 2N N H 4NO 32N, UV 2B UV 2B N H + 4 2N V cm ax Jm ax UV 2B N H + 4 2N V cm ax J m ax NO - 3 2N N H 4NO 32N N H 4 + 2N UV 2B UV 2B UV 2B N H 4 + 2N NO 3-2N N H 4NO 32N UV 2B, UV 2B R ubisco,,, UV 2B, N H 4 + 2N, UV 2B N H 4NO 32N UV 2B UV 2B INFL UENCE OF UV-B RAD IAT ION ON PHOTOSY NTHESIS AND N ITROGEN UT IL IZAT ION OF M USA PARAD IS IACA GROW N IN D IFFERENT N ITROGEN SOURCES SUN Gu2Chou ZHAO P ing and ZEN G X iao2p ing (S ou th Ch ina Institu te of B otany, the Ch inese A cad emy of S ciences, Guang z hou ) PEN G Shao2L in (Guang z hou B ranch, the Ch inese A cad emy of S ciences, Guang z hou ) Abstract A pp roxim ately m axim um pho to synthetic rate w as attained in leaves of M usa p arad isiaca grow n in NO N, N H 4 2N and N H 4NO 32N. UV 2B radiation induced decreases of pho to synthetic rate, apparent quantum yield and efficiency of energy conversion in p lants grow n w ith all three nitrogen sources, but to different degrees. Expo sure to UV 2B radiation resulted in low er levels of leaf dry m ass per area and leaf nitrogen content per unit leaf dry m ass and a slightly elevated level of chlo rophyll content. H igher levels of V cm ax and J m ax w ere found in + p lants grow n w ith N H 4 2N relative to either NO 32N, o r N H 4NO 32N. A stronger decrease in V cm ax and J m ax w as + observed in p lants grow n in N H 4 2N w ith expo sure to UV 2B radiation, indicating a greater sensitivity to UV 2B radiation. UV 2B radiation induced a change in the carbon: hydrogen and carbon: nitrogen ratio s and m ay induce a change in n itrogen u tilization, carbon and n itrogen m etabo lism and acid2base regu lation. D ecreases in the partitioning coefficient fo r leaf nitrogen in R ubisco and in bioenergetics w ere found w ith increasing expo sure to UV 2B radiation w hich m ay cause a decrease in the synthesis of these compo sitions and in pho to synthetic rate. T he results show that p lants grow n in different nitrogen2sources had the ability to vary their responses to UV 2B + radiation. N H 4 2N supp ly m ay act to increase relative pho to synthetic param eters and m ay be capable of being subjected to mo re severe UV 2B radiation impact. L ow er sensitivity to UV 2B radiation m ay occur fo r nitrogen lim ited p lants o r tho se w ith N H 4NO 32N as a m ajo r nitrogen2source. Key words M usa p arad isiaca, D ifferent nitrogen sources, UV 2B radiation, Pho to synthetic param eters, N itrogen partition ing : : : ( ) (980952) (KZ9512B12110)

15 318 25, CO 2, CO 2, B (UV 2B, nm ) UV 2B PA R (150 Λm o l m - 2 s - 1 ) (Caldw ell et a l., PA R (700 Λm o l m - 2 s - 1 ) A n, 1998),UV 2B CO 2 (P i< 1918 pa) A n2p i UV 2B, (, DNA ), (R d, Λm o l m - 2, UV 2B s - 1 ) CO 2 (# 3, pa) PA R (1100 Λm o l m - 2 s - 1 ) DNA (Stride et a l., Ca A n2p i 1994; M azza et a l., 1999) von Caemm erer Farquhar (1985),,, PA R CO 2 R ub isco CO 2, (A nc) : A nc = V c m ax (P i - # 3 ) UV 2B P i + K c (1 + O gk o) - R (1) d, UV 2B V c m ax, O O 2 ( pa) (Jo rdan & O gren, UV 2B UV 2B 1984), K c K o R ub isco, ( 4014 pa pa) (L ong, 1991) CO 2 (6115 pa) PA R,,, R ubp, R ubp, (A nj), A nj = J (P i - # 3 ) 4 (P i + 2# 3 ) - R (2) d, J, Farquhar W ong (1984), J 1 (J m ax), ΗJ 2 - (ΑPA R + J m ax)j + ΑPA R J m ax = 0 (M usa p a rad isiaca) (3), 1, Η, 0195 Α, [m o l m o l ( ) ], Λm o l m - 2 s - 1 ( ), UV 2B C CO 2, ( ) (6115 pa) A n2pa R UV 2B 415 ΛW cm - 2, 3,, (ΑA ), ΑA 50 d,, - (, 1 ) (Kellom ak i &W ang, 1997) mm o l L - 1 N KNO 3 (N H 4) 2SO ΑA 4 N H 4NO 3, = (1 - # 3 gp i) 4 (1 + 2# 3 (4) gp i) 011 mm o l L - 1 KH 2PO 4, 1 mm o l L - 1, CaC l2, 1 mm o l L - 1 K 2SO 4, 015 mm o l L , (M A, g M gso 4, 50 mm o l L - 1 KC l ph m - 2 ), 80%, d L ICOR Beckm an D u27 (C c, CO 2 (Ca, 6116 pa) mm o l ch l g - 1 ),, (PA R, Λm o l m - 2 s - 1 ) (A n,, Perk in E lm er 2400 CHN SgO Λm o l m - 2 s - 1 ), A n2pa R N m

16 3 : UV 2B 319 (g g - 1 ), R ub isco (P R ) (F riend, 1995) :, NO 3-2N, PA R V cm ax P R = (5) 450 Λm o l m - 2 s - 1, A n Λm o l 6125V crgm A gn m m - 2 s - 1, NO 3-2N A n2pa R 6125 (g ( R ub isco (g N in R ub isco ) - 1 ), V cr R ub isco 1),, R ub isco R ubp UV 2B, (Λm o lco 2 (g R ub isco) - 1 s - 1 ) (F riend, 1995) 01005, 15%, UV 2B NO 3-2N (ΑA, m o lco 2 m o l - 1 ) N H f, NAD P (H arley & P B = J m ax 8106 J m cgm A gn m (6) J m c m o l m o l Λm o l 0112 g N, N H 4 + 2N NO 3-2N 8106 Λm o l, N, UV 2B m N H 4NO 32N (P L ) (H iko saka & T erash im a, 1995) : 1, PA R A n PA R N, T enhunen, 1990) NO 3-2N (01022) UV 2B NO 3-2N ( 9% ), (P B ) (Evan s & Seem ann, 1988) : N H 4NO 32N NO 3-2N,UV 2B ( 4), 212 UV 2B C c P L = (7) (V cm ax) N m g CB (J m ax) C c (mm o l ch l g - 1 ), CB 2, CO 2 (Ca, pa), 5179 CO mm o l ch l (gn ) (P i, pa), A n (Kellom ak i &W ang, 1997) NO 3-2N A n2p i 3,, , UV 2B SD 32%, N H 4 + 2N 2 57%, UV 2B, 211 UV 2B N H 4NO 32N, NO 3-2N N H 4 + 2N F ig. 1 UV 2B 1 T he response of pho to synthetic rate (A n) to the incident intensity (PA R ) in leaves of M usa p arad isiaca grow n in different nitrogen sources under supp lem entary UV 2B radiation Contro l 222 UV 2B UV 2B radiation a: NO 3-2N b: N H 4 + 2N c: N H 4NO 32N

17 F ig. 2 UV 2B CO 2 2 T he response of pho to synthetic rate (A n) to intercellular CO 2 partial p ressure (P i) in leaves of M usa p arad isiaca grow n in different nitrogen sources under supp lem entary of UV 2B radiation 2 1 T he legends of F ig. 2 are the sam e as tho se in F ig. 1 a, b, c: 1 See F ig. 1, A n2p i, R d # 3 ( 2) (Λm o l (CO 2 ) m - 2 s - 1 pa - 1 CO 2) N H 4NO 32N ( 2), UV 2B,, CO 2 N H 4 + 2N (A nc), (1) 1, PA R (700 Λm o l m - 2 s - 1 ) P i, NO 3-2N, N H 4 + 2N, N H 4NO 32N A n2p i (P i< 1918 pa) PA R (150 Λm o l m - 2 s - 1 ) N H 4 + 2N, 46% (R d, Λm o l m - 2 UV 2B s - 1 ) CO 2 (# 3, pa), UV 2B NO 3-2N N H 4 + 2N R d # 3, N H 4 + 2N UV 2 PA R (700 Λm o l m - 2 s - 1 ) P i (V c m ax) 2N V c m ax B, # 3 PA R, R d, PA R,, R ubp NO 3 - V c m ax ( 2) UV 2B V c m ax, N H 4NO 32N, UV 2B V c m ax P i PA R, R ubp 1 UV-B A n-p i (P i< 1918 pa) T able 1 T he relationsh ip betw een A n and P i (P i< 1918 pa) in leaves of M usa p arad isiaca grow n in different nitrogen sources and supp lem entary UV 2B radiation under higher and low er light conditions N 2sources NO 3-2N N H 4 + 2N N H 4NO 32N T reatm ent Contro l UV 2B UV 2B radiation Contro l UV 2B UV 2B radiation Contro l UV 2B UV 2B radiation PA R (Λmo l m - 2 s - 1 ) A n= ap i+ b r H igher PA R 700 A n= P i Low er PA R 150 A n= P i H igher PA R 700 A n= P i Low er PA R 150 A n= P i H igher PA R 700 A n= P i Low er PA R 150 A n= P i H igher PA R 700 A n= P i Low er PA R 150 A n= P i H igher PA R 700 A n= P i Low er PA R 150 A n= P i H igher PA R 700 A n= P i Low er PA R 150 A n= P i

18 3 : UV 2B UV-B (V cm ax) T able 2 T he m axim um rate of carboxylation (V cm ax) in leaves of M usa p arad isiaca grow n in different nitrogen sources under supp lem entary UV 2B radiation T reatm ent A nc (Λmo l m - 2 s - 1 ) P i (pa) # 3 (pa) R d (Λmo l m - 2 s - 1 ) V cm ax (Λmo l m - 2 s - 1 ) NO 3-2N Contro l UV 2B UV 2B radiation N H 4 + 2N Contro l UV 2B UV 2B radiation N H 4NO 32N Contro l UV 2B UV 2B radiation A nc: R ubisco Rate of pho to synthesis lim ited by Rubisco P i: CO 2 Intercellular partial p ressure of CO 2 # 3 : CO 2 CO 2 compensation po int in absence of resp iration in the ligh t R d: Resp iration rate in the ligh t V c m ax: L eaf m axim um rate of RuBP carboxylation PA R (J, Λm o l m - 2 s - 1 ) (J m ax) ( N H 4 + 2N, (3) ), N H 4 + 2N (N m, g g - 1 ) (C c, mm o l ch l J m ax, NO 3-2N N H 4NO 32N g - 1 ), UV 2B, J m ax UV 2B J m ax, NO 3-2N N H 4 + 2N, UV 2B UV 2B NO 3-2N N H 4 + 2N, ( 6), J m ax N H 4NO 32N (, UV 2 3) B, N H 4 + 2N UV 2B N H 4NO 32N (, electron s quan ta - 1 ) ( 4) 2N NO 3-2N N H 4 + 2N, UV 2B UV 2B C: H, C: H, 15% 23% 213 UV 2B, N H 4 + 2N C: N, NO - 3 2N N H 4NO 32N UV 2 5, NO 3-2N B (M A g m - 2 ) N H 4 + 2N N H 4NO 32N UV 2B M A, NO 3-2N NO 3 -, UV 2B 3 UV-B (J m ax) Table 3 The m axim um rate of pho to synthetic electron transpo rt in leaves of M usa p arad isiaca grow n in different nitrogen sources under supp lem entary UV 2B radiation A nj P i PA R J Jm ax N 2sources T reatm ent (Λmo l m - 2 s - 1 ) (pa) (Λmo l m - 2 s - 1 ) (Λmo l m - 2 s - 1 ) (Λmo l m - 2 s - 1 ) NO 3-2N Contro l UV 2B UV 2B radiation N H 4 + 2N Contro l UV 2B UV 2B radiation N H 4NO 32N Contro l UV 2B UV 2B radiation A nj: R ubp Rate of pho to synthesis lim ited by RuBP regeneration PA R : Incident intensity J : R ate of electron transpo rt Jm ax: M axim um rate of electron transpo rt P i: 2 See T able 2

19 UV-B 5 UV-B T able 4 T he effect of UV 2B radiation on the apparent quantum yield and the efficiency of light energy conversion in leaves N 2sources NO 3-2N N H 4 + 2N N H 4NO 32N of M usa p arad isiaca grow n in different nitrogen sources under supp lem entary UV 2B radiation T reatm ent 1) 2) Contro l ) UV 2B Contro l ) UV 2B Contro l ) UV 2B ) A pparent quantrum yield (ΑA, Λmo l CO 2 Λmo l pho tons - 1 g 2) Efficiency of ligh t energy conversion (, electrons quanta - 1 ) 3) UV 2B radiation (M A) (N m ) (Cc) T able 5 T he effects of UV 2B radiation on dry w eigh t per N 2sources NO 3-2N N H 4 + 2N N H 4NO 32N leaf area (M A ), nitrogen content (N m ) and T reatm ent chlo rophyll content (C c) 2) 3) 1) Contro l UV 2B 4) Contro l UV 2B 4) Contro l UV 2B 4) ) L eaf dry m ass per area (M A, g m - 2 ) 2) L eaf nitrogen content per unit leaf dry m ass (N m, g g - 1 ) 3) Ch lo rophyll content (Cc, mmo l ch l g - 1 ) 4) UV 2B radiation 6 UV-B T able 6 Changes of content of carbon, hydrogen and carbon2nitrogen ratio in leaves of M usa p arad isiaca grow n in different nitrogen sources and expo sed to UV 2B radiation N 2sources T reatm ent Carbon (% ) H ydrogen (% ) C: H C: N NO 3-2N N H 4 + 2N N H 4NO 32N Contro l UV 2B UV 2B radiation Contro l UV 2B UV 2B radiation Contro l UV 2B UV 2B radiation UV 2B UV 2B P L, UV 2B 7, 2N N H 4 + 2N NO 3 -, R ub isco, 2N N H 4NO 32N, 20% 3 18%, R ub isco NO 3-2N N H 4 + 2N P R UV 2B R ub isco, R ub isco, 2N N H 4 + 2N N H 4NO 32N N H 4 + 2N NO 3-2N N H 4 + 2N Y in R aven (1997) NO 3-2N N H 4 + 2N, NO 3-2N N H 4 + 2N N H 4NO 32N N H 4NO 32N UV 2B, P B UV 2B P B P R UV 2B M ep sted (1996) UV 2B R ub isco UV 2B NO 3-2N P L, (M azza et a l., 1999) UV 2B N H 4 + 2N N H 4NO 32N, UV 2B N H 4 + 2N N H 4NO 32N P L NO 3 -, NO 3 - R ub isco

20 3 : UV 2B UV-B T able 7 T he effect of UV 2B radiation on the nitrogen partition in components of pho to synthetic cycle in leaves of M usa p arad isiaca grow n in different nitrogen sources under supp lem entary UV 2B radiation N 2sources NO 3-2N N H 4 + 2N N H 4NO 32N Coefficient UV 2B UV 2B UV 2B Contro l UV 2B radiation Contro l UV 2B radiation Contro l UV 2B radiation P R (100) (8511) (100) (7510) (100) (8311) P B (100) (6614) (100) (5716) (100) (8418) P L (100) (12916) (100) (13613) (100) (13319) P R: R ubisco Partitioning coefficient fo r leaf nitrogen in Rubisco PB : Partitioning coefficient fo r leaf nitrogen in bioenergetics PL : Partitioning coefficient fo r leaf nitrogen in thylako id light - harvesting components, UV 2B R bcs R ub isco V cm ax J m ax R d N H 4 + 2N (bca) (Cheng et a l., 1997),, V cm ax J m ax R ub isco, UV 2B NO 3-2N N H 4NO 32N, UV 2B R ub isco N H 4 + 2N V cm ax J m ax,, NO 3-2N N H 4NO 32N N H 4 + 2N NO 3-2N N H UV 2B NO 3-2N N H 4NO 32N N N H 4NO 32N UV 2B, UV 2B, UV 2B UV 2B (Searles et a l., 1995) UV 2B (M ep sted et a l., 1996; Scheib le et a l., 1997),, N H 4NO 32N, UV 2B, R ubp R ubp N H 4 + 2N, Farquhar (1980) N H 4 + 2N, N H 4NO 3,, V cm ax R ubp (Geiger et a l., 1999),, V cm ax R ub isco (Farquhar & von Caemm erer, 1982),, UV 2B C: H C: N, UV 2B R ub isco, R ub isco, UV 2B C: N, UV 2B R ub isco UV 2B, UV 2B, # 3 P i, N H 4 + 2N, UV 2B N H 4 + 2N Caldw ell, M. M., L. O. B jo rn, J. F. Bo rnm an, A. H. T eram ura & M. Tevini Effects of increased so lar ultravio let

21 radiation on terrestrial eco system. Journal of Pho tochem istry and Pho tobio logy, 46B: Cheng, S. H., D. M o rre & J. R. Seem an Effects of sho rt2 and long2term elevated carbon dioxide in the exp ression of ribulo se 1, 52bispho sphate carboxylasegoxygenase genes and carbohydrate accum ulation in leaves of A rabidop sis thaliana (L. ) H eynh. P lant Physio logy, 116: Evans, J. R. & J. R. Seem ann The allocation of p ro tein nitrogen in the pho to synthetic apparatus: co sts, consequences, and contro l in pho to synthesis. In: B riggs, W. R. ed. P roceedings of the C. S. F rench sympo sium on pho to synthesis held in Standfo rd, Califo rnia, July 17223, P lant B io logy 8. N ew Yo rk: A lan R. L iss. Inc Farquhar, G. D. & S. C. W ong A n emp irical model of stom atal conductance. A ustralian Journal of P lant Physio logy, 11: Farquhar, G. D. & S. von Caemm ereṙ M odeling of pho to synthetic response to environm ental condition. In: L ang, O. L. ed. Encyclopedia of p lant physio logy. H eidelberg: Sp ringer2v erlag. V o l. 12B Farquhar, G. D., S. von Caemm erer & J. A. Berry A biochem ical model of pho to synthetic CO 2 assim ilation in leaves of C3 species. P lanta, 149: F riend, A. D PGEN : an integrated model of leaf pho to synthesis, transp iration, and conductance. Eco logical M odelling, 77: Geiger, M., V. H aake, F. L udew ig, U. Sonnew ald & M. Stitṫ The nitrate and ammonium nitrate supp ly have a m ajo r influence on the response of pho to synthesis, carbon m etabo lism, nitrogen m etabo lism and grow th to elevated carbon dioxide in tobacco. P lant, Cell and Environm ent, 22: H arley, P. C. & J. D. Tenhunen M odeling the pho to synthetic response of C3 leaves to environm ental facts. In: Boo te, K. ed. M odeling crop pho to synthesis2from biochem istry to canopy. CSSA special publication N o. 19. M adison, W I: Am erican Society of A gronom y and C rop Science H iko saka, K. & İ Terashim a A model of the acclim ation of pho to synthesis in leaves of C3 p lant to sun and shade w ith respect to nitrogen use. P lant, Cell and Environm ent, 18: Jo rdan, D. B. & W. L. O gren The CO 2gO 2 specificity of rubulo se 1, 52bipho sphate carboxylasegoxygenase, dependence on ribulo se bipho sphate concentration, ph and temperature. 161: P lanta, Kellom aki, S. & K. Y. W ang Effects of elevated O 2 and CO 2 concentration on pho to synthesis and stom atal conductance in sco ts p ine. P lant, Cell and Environm ent, 20: Long, G. P M odification of the response of pho to synthetic p roductivity to rising temperature by atmo spheric CO 2 concentration: has it impo rtance been under estim ated: op inion. P lant, Cell and Environm ent, 14: M azza, C. A., D. Battista, A. M. Zim a, M. Szw arcber2 B racchitta, C. V. Gio rdano, A. A cevedo, A. L. Scopel & C. L. Ballare The effects of so lar ultravio let2b radiation on the grow th and yield of barley are accompanied by increase DNA dam age and antioxidant responses. 22: P lant, Cell and Environm ent, M ep sted, R., N. D. Paul, J. L. Stephen, J. C. Co rlett, S. N ogues, N. R. Baker, H. G. Jones & P. G. A yres Effects of enhanced UV 2B radiation on pea (P isum sativum L. ) grow n under field conditions in the U K. 2: Global Change B io logy, Scheible, W. R., M. L anerer, E. D. Schulze, M. Cabbehe & M. Still A ccum ulation of nitrate in the shoo t acts as signal to regulate shoo t2roo t allocation in tobacco. The P lant Journal, 11: Searles, P. S., M. M. Caldw ell & K. W inteṙ The response of five trop ical species to so lar ultravio let2b radiation. Am erican Journal of Bo tany, 82: Stride, A., W. S. Chow & J. M. A nderson UV 2B dam age and p ro tection at the mo lecular level in p lants. Research, 39: Pho to synthesis von Caemm erer, S. & G. D. Farquhaṙ Som e relationship betw een the biochem istry of pho to synthesis and gas exchange of leaves. P lanta, 165: Yin, Z. H. & J. A. Raven A comparison of the impact of various nitrogen sources on acid2base balance in C3 T ritieum aestivum L. and C4 Z ea m ay s L. p lants. Journal of Experim ental Bo tany, 307: : :

22 2001, 10(3): 198~200 Soil and Environmental Sciences X S158 A Gray relation grades analyses of soil fertility of eucalyptus plantation in Zixing, Hunan province LI Yue-lin 1 LI Zhi-hui 2 LI Zhi-an 1 REN Hai 1 1: South China Institute of Botany, Academia Sinica, Guangzhou , China; 2: Central South Forestry University, Zhuzhou , China Abstract: By gray relation analysis, soil fertility was evaluated according to the nutrient contents and the enzyme activities of Eucalyptus plantation soils. The applicable range must be further investigated before soil enzyme activities are used as appraisal indictors for soil fertility in Eucalyptus plantation. Key words: gray relation analysis; soil fertility; eucalyptus plantation soil yuelin@scib.ac.cn

23 Vol.10 No /m ph 290~ ~ ~ ~ ~ ~ ~ ~6.4

24 200 Vol.10 No.3

25 Ξ ( ) ( ), ( IGBP) : ( IHDP), (WCRP) (DIVERSITAS) ; CO ; C ; ;, 80 ( ), ; ; IGBP Moore ; : ; 12,800, : ;,, : ; ;, ; : ;, ; 50 4 : ;,, ;,, : Ξ, :

26 2001,7 (3) : Chin J Appl Environ Biol = ISSN X ( M agnolietia pachyphylla) ( ) ( ), ( A/ mol mol - 1 ) , 40 % 20 %, A % ( / mol mol - 1 ) , 20 % % %.,40 %. 20 %,. 40 % 20 % 3 R d. 40 %. 20 % R d. 40 % 20 V cmax J max. J max V cmax, V cmax J max. 40 % J max. 20 %, CO 2 40 %..,40 % ; ; CLC Q Q VARIATION IN L IGHT RESPONSE OF PHOTOSY NTHESIS IN L EAVES OF MAGNOL I ETIA PACHYPHYLLA GROWN ALONGLIGHT GRADIENTS 3 SUN Guchou, ZHAO Ping, ZEN G Xiaoping & PEN G Shaolin ( South Chi na Instit ute of Botany, Chi nese Academy of Sciences, Guangzhou ) ( 1 Guangz hou B ranch, Chi nese Academy of Sciences, Guangzhou ) Abstract Apparent photosynthetic quantum yield in leaves of M agnolietia pachyphylla grown at 40 % daily light intensity was mol mol - 1. However, the values of and mol mol - 1 were found in leaves of saplings grown at full daily light intensity and 20 % daily light intensity, respectively. The efficiency of light energy conversion was mol mol - 1 for saplings grown at 40 % daily light intensity, % and % higher than that of saplings grown at the full daily and at 20 % daily light intensities. It may be suggested that light condition with 40 % daily light intensity will enhance the efficiency of light energy conversion. On the contrary, the shady condition caused t he decrease of light energy conversion. 40 % daily light intensity can increase mi2 tochondrial respiration in light and the CO 2 compensation point in the absence of mitochondrial respira2 tion. And higher V cmax and J max were found in leaves of saplings grown at 40 % daily light intensity in comparison with those of saplings grown at the full or 20 % daily light intensity. Light condition may modify the balance between V cmax and J max. 40 % daily light intensity can promote the increase of photo2 synthetic electron transport rate. And the saplings grown at daily or 20 % daily light intensity had higher sensitivity of response to CO 2 concentration and relative humidity in atmosphere in comparison than those : : (No : ) ( KZ9512B12110) (No :980952) 3 3 (Corresponding author)

27 214 7 of saplings grown at 40 % daily light intensity. Light condition under which saplings grow may modify t he stomatal response to environmental factors. The environmental condition wit h 40 % daily light inten2 sity may be the best selection for transplantation of M agnolietia pachyphlla. Fig 2, Tab 5, Ref 15 Keywords CLC Q M agnolieta pachy phylla ; light gradient ; p hotosynt hetic response Q , CO 2 C a, CO 2. P AR (840 mol m - 2 s - 1 ) P AR (200 mol m - 2. s - 1 ) P n. CO 2 ( C i < 180 L, L - 1 ) P n - C i, /., ( R d / mol m - 2 s - 1 ). CO 2 ( 3 / mol m - 2 s - 1 ). P AR (840 mol m - 2 s - 1 ) CO 2 P n 2. Niinements, Kull Tenhunen (1998) C i, CO 2 P AR ( 200 mol m - 2 s - 1 ) P [1 ] n 2 P AR... de Pury Farquhar (1997) [4 ],, ( P n ) Rubicos ( P nc ). [4,5 ( P (Magnoliaceae) nj ) ]. [2 ] P n = min{ P nc, P nj } - R d., P nc = [ V cmax ( C i - 3 ) / ( C i + K ) ] - R d (1)., [3. ] K = K c (1 + O/ Ko) P nj = [ J ( C j - 3 ) / 4 ( C i ) ] - R d (2) 39 ( R d ( mol m - 2 s - 1 ), ),. C i CO 2. 3, CO, 2 ( L L - 1 ). O. O 2 (210 mmol mol - 1 ). K Rubico ( mol m - 2 s - 1 ). K c K o Rubisco 1 c - Ha/ R Tk 1. 1 K c(o) = e ( M agnolietia pachyphylla) C, K c K o , 1,, H a, R 1/ 2 Hoagland,. ( KJ mol - 1 K - 1 ), K c K o H a %, kj mol - 1[7,8 ]. t/ C, T/ 20 %. k t/ C, V cmax ( mol m - 2 CK. Li - 188B s - 1 ),J ( mol m - 2 s - 1 ), J 3, 0. 4g0. 2g J 2 - ( P A Rle + J max ) J + P ARle J max = 0 (3) 60 d, 5. [6, ] :. ( J max / mol - 2 s - 1 ) [9 ] : P A Rle = [ (1 - f ) / 2 ] P A R 1. 2, Licor ( P AR / 0. 9, P ARle P AR mol m - 2 s - 1 ) ( P n / mol m - 2 ( mol - 2 s - 1 ). f (0. 15). s - 1 ), P n 2 P AR., (0. 85) [10 ].

28 3 : ( Magnolietia pachyphylla) 215 P n - P AR R d. R d ( A/ mol mol - 1 ). ( / mol mol - 1 ) : = 4 A ( / C i ) (1-3 / C i ) (4), CO 2,Nijs (1997) [11 ] Ball (1987) [12 ]. Ball, Woodrow Berry (1987) [12 ] Harley (1992) [13 ], g s P n ( R H ) CO 2 ( C a ) : g s = g o + g i [ P n ( R H / C ) ] (5) 1 R d g o P AR = 0, P n = 0 CO 2. 3, Fig. 1 Photosynthetic response to light intensity in leaves of, g i 1 ( P n ) CO 2 ( C i < 180 L L - 1 ) 1,, Tabel 1 The relationship between P n and C i ( C i < 180. P n 2 P AR along light gradients measured at tow light levels., ( A ) mol mol % 20 % mol mol %, ( P > 0. 05), 20 %. 20 % ( P < 0. 01). 2.2 (840 mol m - 2 s - 1 ) CO 2 ( V cmax ), P n C i. RuBP, ( 840 ( P nc ), (1) mol m - 2 s - 1 ) (200 mol m - 2 s - 1 ) ( V cmax ). 2, 40 % P n C i ( C i < 180 L L - 1 ) ( ( V cmax ) mol 1). m - 2 s - 1, 40 % %, ( R d / mol m - 2 s - 1 ) 20 % %. CO 2 ( 3 / L L - 1 ),, 40 %, mol m - 2 s L L - 1 ( 2),. 20 %, 40 % 20 %, R d mol M agnlietia pachyphylla grown at different light conditions L f full daily light intensity L % daily light intensity L 0. 2 daily light intensity L L - 1 ) in leaves of M agnolietia pachyphylla grown Light Light level gradient ( L l / mol m - 2 s - 1 ) y = ax + b ( P n = ac i + b) L f 840 P n = C i P n = C i L P n = C i P n = C i L P n = C i P n = C i ( P < 0. 05). m - 2 s - 1, mol m - 2 s , R d 40 % CO 2 ( C a > 450 L L - 1 ). Villar (1995) [14 ], CO 2 RuBP,.,, P n, P AR, P n. R d RuBP ( P nj ), ( H a. (2) ( J / mol r 2

29 ( V cmax ) Table 2 Maximum rate of carboxylation in leaves of M. pachyphylla grown along light gradients P n / C i / ( 3 / R d / V cmax / Light gradient mol m - 2 s - 1 L L - 1 L L - 1 mol m - 2 s - 1 mol m - 2 s - 1 L f L 0. 4 L CO 2 Table 3 The rate of photosynthetic electron transport in leaves of M. pachyphylla grown along light gradients 2, P AR = 0, P n = 0, P AR / P n / C i / J/ Light gradient mol m - 2 s - 1 mol m - 2 s - 1 L L - 1 mol m - 2 s - 1 L f mol m - 2 s - 1, 40 % L mol m - 2 s - 1,., L % 4,, Table 4 Maximum rate of photosynthetic electron transport in leaves of M. pachyphylla,. grown along lighe gradients P AR / P ARle / J max / Light gradients mol m - 2 s - 1 mol m - 2 s - 1 mol m - 2 s - 1 L f L 0. 4 L m - 2 s - 1 ) ( 3). 4, g 0, , g 0,. g i C a (CO 2 ) R H. ( ) (20 % ), g i 40 %, C a (CO 2 ) R H. 3 ( P AR le / mol m - 2 s - 1 ) 3. 1, ( J max / mol m - 2 s - 1 )., 40 %,, ( J)., 20 % % 41. ( McMurtric, 1992) [15 ]. 4 %, ( J max ), 20 %. 40 %. ( P < 0. 01)., (20 % ),,.. J max., 2. 4,. 5, 40 % 20 %, 40 % 20 %. 40 %,,. 20 %. 40 %,.. 5 ( A) ( ) Table 5 The apparent quantum yield ( A ) and efficiency of light energy conversion( ) in leaves of M agnolietia pachyphylla grown along light gradients P AR / P n / C i / A / / Light gradients mol m - 2 s - 1 mol m - 2 s - 1 L L - 1 mol mol - 1 mol mol - 1 L f L 0. 4 L

30 3 : ( Magnolietia pachyphylla) 217 Fig. 2 The relationship among stomatal conductance, CO 2 concentration and relative humidity in atmosphere. Regression equation : g s = g 0 + g 1 [ P n ( R H / C a ) ] L f L 0. 4 L % J max V cmax. J max RuBP., J max V cmax., RuBP, 40 % J max. 20 %, Rubisco. 40 %. 3. 3,..., 40 %. g % CO 2... CO 2,. 40 %,.,DW g m - 2,,. 40 % 2 CO 2.. : g s = g 0 + g i [ P n ( R H / C a ) ] 1 Niinements U, Kullo, Tenhunen JD. An analysis of light ef2 fects on foliar morphology, physiology, and light interception in temperate deciduous woody species of contrasting shade - tolerance. T ree physiology. 1998, 18 : % 2 Liu Yuhu( ),Xia Nanhe( ), Yang Huiqiu ( ( P > 0. 05), 20 % ). The origin evolution and phytogeography of magnoli2, 40 % aceae. J T rop & S ubt rop Bot ( )., 1995,3 (4) :1 12,40 %. 3 Fu Liguo ( ). The red book of plant in China rate and 3 endangered plants. Beijing : Science Press,1992 C i, 4 de Pury D GG, Farquhar BD. Simple scaling of photosynthesis V cmax J max R d, from leaves to canopies without the errors of big - leaf mod2 3 els. Plant, Cell & Environment. 1997,20 : V cmax J max R d. 40 % 5 Harley PC, Tenhunen JD. Modeling the photosynthetic re2 20 % sponse of C 3 leaves to environment factors. In : ed. Boote KJ. V cmax J max,j max V cmax 2, Modeling crop photosynthesis from biochemistry to canopy,

31 218 7 CSSA special publication No. 19. Madison : American Society of Agronomy and Crop Science Society of America, Ninements U, Tenhunen JD. A model separating leaf struc2 tural and physiological effects on carbon gain along light gradi2 ents for the shade - tolerant species Acer saccharum. Plant, Cell & Environ, 1997,20 : Jordan DB, Ogren WL. The CO 2 / O 2 specificity of ribulose 1, 5 - bisphosphate carboxylase/ oxygenase. Dependence on ribu2 lose bisphosphate concentration, p H and temperature. ta. 1984,161 : Plan2 8 Nolan WG, Smillie RM. Multi - temperature on Hill reaction activity of barley chloroplasts. Biochim Biophys Act. 1976, 440 : Farquhar GD, Wong SC. An empirical model of stomatal conductance. A ust r J Plant Physiol. 1984,11 : Medlyn BC, Badeck FW, de Pury D GG Barton CVM, Broadmeadow M, Ceulemans R, de Angelis P, Forstreuter M, Jach ME, Kellomaki E, Laitat E, Marek M, Philippot S, Rey A, Strassemeyer J, Laitinen K, Liozon K, Portier B, Roberntz P, Wang K, Jarvis PG. Effects of elevated CO 2 on photosynthesis in European forest species : a meta - analysis of model parameters. Plant, Cell & Environ. 1999,22 : Nijs I, Ferrris R, Blum H, Hendrey G, Impens I. Stomatal regulation in a changing climate : a field study using free air temperature increase ( FA TI) and Free Air CO 2 Enrichment ( FACE). Plant, Cell & Environ. 1997,20 : Ball J T, Woodrow L E, Berry JA. A model predicting stom2 atal conductance and its contribution to the control of photo2 synthesis under different environmental conditions. In : ed. Giggins I. Progress in Photosynthesis Research : Proceed2 ings of the International Photosynthesis Congress. Netherlands : Martinus Nifhoff Dordrecht, Harley DC, Thomas RB, Reynolds J F, Strain BR. Model2 ing photosynthesis and water relations of cotton grown in el2 evated CO 2. Plant, Cell & Environ. 1992,15 : Villar R, Held AA, Merino J. Dark leaf respiration in light and darkness of an evergreen and a deciduous plant species. Plant Physiol. 1995, 107 : Mcmurtrie RE, Leuning R, Thompson WA, Wheeler AM. A model of canopy photosynthesis and water use incorporat2 ing a mechanistic formulation of leaf CO 2 exchange. Forest Ecol & M anag. 1992, 52 :

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37 CHIN ESE JOURNAL OF APPL IED ECOLO GY,J un. 2001,12 (3) CO (, ) (, ) CO 2 ( l L - 1 ) 1, ( Pn, mol m - 2 s - 1 ) , CO 2 (356 30l l L - 1 ) %, CO 2 8, Pn CO %, CO 2. CO 2 (Rd), CO 2. (Vcmax) (J) CO % %, (,mol CO 2 mol - 1 ), CO , CO CO 2 44 %. (electrons quanta - 1 ) CO 2 Rubicos ( P R ) ( P B ) ( P L ) CO 2, CO 2 (8 ),. CO 2 N (2001) Q949 A Influence of elevated atmospheric CO 2 concentration on photosynthesis and leaf nitrogen partition in process of pho2 tosynthetic carbon cycle in Musa paradisiaca. SUN Guchou,ZHAO Ping,ZEN G Xiaoping ( South China Institute of Botany, Chinese Academy of Sciences, Guangz hou ),PEN G Shaolin ( Guanz hou B ranch, Chinese Academy of Sciences, Guangz hou ). 2Chin. J. A ppl. Ecol.,2001,12 (3) : The photosynthetic rate ( Pn) in leaves of M usa paradisiaca grown under elevated CO 2 concentration ( l L - 1 ) for one week was mol m - 2 s - 1,22. 1 % higher than that under ambient CO 2 concentration,while under elevated CO 2 concentration for 8 week,the Pn decreased by %. It can be inferred that the photosynthetic acclimation to elevated CO 2 concentration and the Pn inhibition occurred in leaves of M. paradisiaca. The respiration rate in light (Rd) was lower in leaves under higher CO 2 concentration,compared with that under ambient CO 2 concen2 tration. If the respiration in light was not included,the difference in CO 2 compensation point for the leaves of both plants was not significant. Under higher CO 2 concentration for 8 weeks,the maximum carboxylation rate(vcmax) and electron transportation rate (J) in leaves decreased respectively by % and %,compared with that under am2 bient CO 2 concentration. The calculated apparent quantum yield ( ) in leaves under elevated CO 2 concentration accord2 ing to the initial slope of Pn/ PA R was reduced to molco 2 mol - 1 quanta,compared with the value of molCO 2 mol - 1 quanta in the control. The efficiency of light energy conversion also decreased from to electrons quanta - 1 in plants under elevated CO 2 concentration. A lower partitioning coefficient for leaf ni2 trogen in Rubisco,bioenergetics and thylakoid light2harvesting components was observed in plants under higher CO 2 concentration. The results indicated that the multi2process of photosynthesis was suppressed significantly by a long2term (8 weeks) higher CO 2 concentration incubation. Key words M usa paradisiaca, Higher CO 2 concentration, Photosynthetic rate, Leaf nitrogen partition. CO 2, [ CO 2 ]/ [ O 2 ], 1 [21 Rubisco ],. CO l L - 1, CO 2,, CO 2 1. CO 2 [18 ]. CO 2,, C 3 1, 5 2 (Rubisco), CO 2, [2,4 ]. CO 2 Rubisco CO 2, 3 ( ) (980952) ( KZ9512B2110) ,