32 9 OOD & MACHINERY 216 9 食品与机械 Vol.32No.9 Sept.216 DOI:1.13652/j.issn.13-5788.216.9.34 Optimizationonultrasonic-microwavecombinedextractionof gypenosidesandsynergeticefectanalysis CHENG Yi-qun JI Hang-yan ZHANG Fan HUANG Yi ZHOU Shou-biao HE Rong-rong JI Ya-li CHEN Chun-hua ( 2413) (Colegeof EnvironmentalScienceand EngineeringAnhui Normal UniversityWuhuAnhui2413China) : peratureat62. extractiontimewas12.8min.undertheoptimalex- Box-Be- tractionconditiontheyieldofgypenosideswas24.8±.37 mg/g.the hnken experimentresultsshowedthattheinteractionbetweenultrasonicandmi- 4 crowavewassignificantandplayedamajorroleintheextractionprocess. Keywords:Gynostemma pentaphylum;saponins;ultrasonic-micro- wavecombinedextraction;synergeticefect (Gynostemma pentaphylum) : (5 W) 613 W [1-2] [3] 145s 62. 12.8 min [4] [5-6] (24.8±.37)mg/g [7-8] [9] [1] [11] [12-13] : ; ; ; [14-15] CO2 Abstract:Optimizationonultrasonic-microwavecombinedextraction ofgypenosidesandtheanalysisonthesynergisticefectbetweenul- trasonicandmicrowavewerestudied.theoptimalextractioncondi- tionofgypenosideswasobtainedbyresponsesurface methodology. Fourfactorsefectontheyieldofgypenosidessuchas microwave powermicrowaveprocessingtimeextractiontemperatureandex- tractiontimewereinvestigated.thesynergisticefectofultrasonic [16-17] andmicrowavewasanalyzedbytheyieldofgypenosidesunderdifer- entextractionconditions.theoptimalextractionconditionofgype- nosidesbyultrasonic-microwavewasultrasonicopen(5 W)micro- wavepowerat613 Wmicrowaveprocessingfor145sextractiontem- 1 : ( :214xmpy14) 1.1 : (1987 ) E-mail:cheng.sheng515@gmail.com 1.1.1 :216-6-27 : ; 135
216 9 Rb1 : 98% ; : 1.1.2 :CW-2 245kHz ~1 W 4kHz 5 W ; :722G ; 1 :DZF-62A Figure1 Efectofmicrowavepoweronyieldofgypenosides ; :FA14 1.2 1.2.1 6 6 2.1.2 2 1.2.2 1min 2min (5 W) 3 [18-19] 2.g 7% 1 2(g/mL) 25mL (1) : 1min 7 2.1.3 3 15 min (2468 3 6 1 W) (2) : 6 W 7 15min (12345 min) (3) : 6W 2min 15min (34567 ) (4) : 6W 2min 6 (5115225 min) 1.2.3 2 DesignExpert8..6 Box-Behnken Figure2 Efectofmicrowaveprocessingtimeonyield ofgypenosides 4 1.2.4 [3] Rb1 : y(od55nm)=2.9275x-.119(r 2 =.9992).4~.2 mg 2 2.1 7 2.1.1 1 3 2 W 6 W Figure3 Efectofextractiontemperatureonyield ofgypenosides 136
32 9 : 2 Table2 Experimentaldesignandresults [2] A B C D /(mg g -1 ) 2.1.4 4 1-1 -1 17.78 5min 2-1 1 18.832 1 min 3 4 1-1 19.686 23.732 5 1-1 19.51 6 1-1 2.687 7 1-1 19.129 8 1-1 21.596 9 1 1 23.224 1 1-1 21.357 11 23.176 12-1 1 2.573 13 23.319 14-1 -1 2.164 15-1 1 21.778 4 16-1 -1 17.927 Figure4 Efectofextractiontimeonyieldofgypenosides 17 1 1 23.119 2.2 18 1 1 23.863 19-1 -1 17.82 ( 1) 2 1 22.364 2 21 1 1 22.93 2.2.1 DesignExpert8..6 22 1 1 22.821 3 23-1 -1 18.785 3 P <.1 24 1 1 21.25 P =.9193>.5 25-1 -1 19.428 R 2.9793 26 23.253 R 2 Adj.9487 27-1 1 21.137 28-1 1 2.469 : 29 1 (P<.1) 1 2.681 (P=.14); 3 2.2.2 (1) 3 ; (P<.1) (2) 4-2 -1 1 2 ( ): 4 Y=23.47+.69A+1.33B+.11C+1.78D-1.5AB- 烄 Y1=23.47+.69A-1.7A 2 ;.49AC +.16AD +.77BC +.12BD +.28CD - Y2=23.47+1.33B-1.6B 2 ; 烅 1.7A 2-1.6B 2-1.1C 2-1.61D 2 (1) Y3=23.47+.11C-1.1C 2 ; (2) 1 烆 Y4=23.47+1.78D-1.61D 2 5 Table1 Factorsandlevelsofresponsesurface methodologyexperiments A B C D 5 4 1 /W /min / /min 1 8 3 7 15 2.2.3 6 2 6 1-1 4 1 5 5 : 137
216 9 3 Table3 ANOVAanalysisfortheresponsesurfacemodified F P A 5.75 1 5.75 113.26 <.1 * * B 21.21 1 21.21 417.8 <.1 * * C.15 1.15 3.2.14 D 38.3 1 38.3 749.15 <.1 * * AB 4.37 1 4.37 86.13 <.1 * * AC.96 1.96 18.84.7 * * AD.1 1.1 2.3.1761 BC 2.39 1 2.39 47.15 <.1 * * BD 5.52E-4 1 5.52E-4.1.9184 CD 3.19E-3 1 3.19E-3.6.856 烄 dy/da=.69-3.4a; dy/db=1.33-3.2b; 烅 dy/dc=.11-2.2c; 烆 dy/dd=1.78-3.22d (3) 6 (3) A 2 18.82 1 18.82 37.73 <.1 * * B 2 16.56 1 16.56 326.18 <.1 * * C 2 7.89 1 7.89 155.37 <.1 * * D 2 16.83 1 16.83 331.46 <.1 * * 112.15 14 8.1 157.82 <.1.71 14.5.33 1.3.35.9193.38 4.1 112.86 28 ** (P<.1);* (P<.5) R 2 =.9793R 2 Adj=.9487 5 Figure5 Singlefactorefectcurve Figure6 6 Marginalefectofasinglefactorcurve 6 (3) ( ) 4 3 4 : A=.23 B=.416 C=.5 D=.553 ; 2.2.4 [21] [22] 7~9 3 7 7 Figure7 Efectofmicrowavepowerandmicrowaveprocessingtimeonyieldofgypenosides 138
32 9 : 8 Figure8 Efectofmicrowavepowerandextractiontemperatureonyieldofgypenosides 9 Figure9 Efectofmicrowaveprocessingtimeandextractiontemperatureonyieldofgypenosides 8 4 Table4 Analysisofultrasonic-microwave 9 synergisticextraction 2.2.5 DesignExpert : 612.91 W / (mg g -1 ) 613 W 2.44min 62. 12.8 min 1 145s 24.8±.37 a 24.2961mg/g 62 12.8min 613 W 145s 613 W 62. 12.8min 3 2 145s 62 17.24±.37 c (24.8±.37)mg/g min 613 W 2.3 3 145s 62 21.31±.21 b 12.8min 613 W 4 145s 13.89±.17d 62 12.8min 4 spss18. Duncan s (P<.5) P<.5 4 ; 1 2 3 12.8 min (P<.5) 1 2 145s 88.5% 71.6% ; 3 4 139
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