Research on the Mycorrhizal Community of Pinus Massoniana Lamb in Wuyishan Nature Reserve Abstract Mycorrhizae is the symbiosisal body of fugus and bo

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(pinus massoniana Lamb) 27 o 33. o 54, 117 o 27 117 o 51 1

Research on the Mycorrhizal Community of Pinus Massoniana Lamb in Wuyishan Nature Reserve Abstract Mycorrhizae is the symbiosisal body of fugus and botanial nutritive root It is the common phenomena of botany in nature Mycorrhizaes are divided into three types:endomycorrhizae,endo-ectomycorrhizae and ectomycorrhizae by the difference of morphological structure In this paper, researches on ectomycorrhizae of Pinus massoniana Lamb in Wuyishan Nature Reserve are done Wuyishan Nature Reserve is located in 27 2

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(Pinus massoniana Lamb.) 1. [1] 1.1 () 27 33-27 54 117 27-117 51 22km 52km 56527ha 1200m 2158m 2500m 110 500 km 1979 1987 8.5 18 C -15 C 1486-2150mm 3000mm 1000mm 78-84% 253-272 700m 700-1050m 1050-1900m 1900m 350-1400m 6

500-1800m 1700-2158m [2] 1.2 (Pinus massoniana Lamb.) 20-25 1 2 12-20 4-7 1-1.5 2-4 4-7 2.5-4 3 4-7 2.5-3 13-16 10.8(9.8-13.8) 92000 85(74-95)% 1100 1000-1300 1300 7

200-1100m 4 - - - - - - - - - 1.3 (mycorrhiza) 1885 Frank 19 Monotropa hypopitys L. 1881 Kamienski Halket(1903) [3],Osborn(1909) [4], Kidston Lang(1921) [5] 100 19 20 Maeda(1954) [6] Gerdmann(1968) [7] 200 1000 Trappel(1962) [8] 100 30 81 535 280 3% [9] (Hymenomyceles) (Gasteromyceles) 8

(Boletus), (Lactarius), (Russula) (Tuber), (Cenococcum) ( VA ) 90% (Endogo naceae) (Glomus,Tul.), (Gigaspora Gerdemannet Trappe), (Acaulospora Gerdeman et Trappe), (Acaulospora Gerdeman et Trappe) (Sclerocystis Berk et Br.) (Pinueae), (Rhodoelendreae) (Monotropaceae) (Pyrolaceae) 1 3 1 50 1 3 1 1 (1983) [11] 23 (1983) [12] 25 (1985) [13] (1986 )[14] (1989 )[15], (1990) [16] (1988)[ 17] (1989) [18] 27 (1991) [19] 11 (1991) [20] (1993)[ 21] 115 (1993) [22] (1994) [23] 11 (1994) [24] 35 (1994) [25] (1997) [26] [10] 9

(1999) [27] 16 51 (1999) [28] (1996-1999) [29] 45 1 3 1 2 (1959) [30] (1959) [31] (1964) [32] (1981) [33] (1983) [34] (1985) [35] (1983) [36] (1988) [37] (1986) [38] (1992) [39] (1991) [40] (1992) [41] (1981) [42] (1981) [43] (1994) [44] (1984) [45] (1989) [46] (1991) [47] (1991) [48] N P K (1989) [49] (1994) [50] (1994) [51] (1997) [52] (1997) [53] 1 3 3 3 (1983) [54] (1991) [55] (1994) [56] (1997) [57] Pt 10

80 VA VA 70 70 1994 200 1989 (MIC) 14, 1 3 2 11

Mycorrihae (NACOM) (ACOM) (ECM)1995 (MIC) 1 3 2 1 Frank1885 Melin(1927) [58] Trappe(1967) [59] Dominik(1969) [60] Zak(1973) [61] 12

Pseudotsuga menzielii( )+Rhizopogon vinicolor Agerer (1987-1997) [62-67] (Picea abies)19 113 (Russula) (Lactarius) 40 80 Agerer CD(DEEMY)Haug I,pritsch K.(1992) [68] (Picea abies)38 12 Kough (1983) [69],Wilson (1983) [70] (FA) Alchwell(1985) [71] (ELISA) VA Sen Hepper(1986) [72] 6 VA VA Wright (1987) [73] (MCABS)VA Henrion (1992) [74] rrna Bianciotto Bonfante(1992) [75] VA DNA Bruns Gerdes(1993) [76] (Suillus) Ianfrance (1993) [77] DNA Wyss Bonfante(1993) [78] VA DNA Erland (1994) [79] (RFLP) 1 2 13

3 Agerer 4 [80] DNA (RFLP) PCR (PCR) (RFLP) PCR DNA(rDNA) PCR (ITS) (IGS) IGS RFLP RFLP DNA(RAPD) DNA 5 ELISA( ) DNA (PCR)ITS IGS RFLP RAPD, DNA 14

DNA 1 3 2 2 Harley&McCready(1950) [81] 32P 32P 2.3-8.9 Melin,E.& Nilsson(1957) [82] 14CO2 14C Moser(1959) [83] 23 Hyppl(1968) [84] 42 85 40% Palmer&Hacskyla(1970) [85] Nye&Zak(1979) [86] [87] Gardaye Allem Benthlen 15

[88] 1 3 2 3 1 [89] 1974 1975 86% 100%97% [87] 1928 27 30 1955 1 (Briscoe,1959) [90] (Mikola,1965) [91] 1 80-100% 50-80% Pt 7 16

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