398, 2 [1], AIDS,, 404% 376%, 52 24%,33%,41% [2] 52,,,,,,, [3],, 1 11 实 验 材 料 ( ),JY92 Ⅱ ( ),MHA ( ),Baird Parker ( ) , 3 (Moniliaalbica

37 / 4 / 2013 7 / / JOURNALOFHEBEINORMALUNIVERSITY/NaturalScienceEdition/ Vol37No4 Jul2013,,, (, 050024) : ( ), 2, ph ;,, 96h 29 220r/min ph71 : ; ; ; :S476 :A :1000-5854(2013)04-0397-05 TheScreeningofAntibacterialAntagonisticActinomycetesand theoptimizationoffermentationprocess ZHANG Hui, YANGJunqing, ZHOU Tianhui, ZHAOBaohua (ColegeofLifeScience,HebeiNormalUniversity,HebeiShijiazhuang 050024,China) Abstract:Actinomycetesthatareantagonistictopathogenicfungi(especialyCandidaalbicans)were screenedfromthesoilinlandthathadbeenplantedcotonperennialyandhadbecomehardenedinshiji- azhuangsuburb,andfromsoilinheavy metal-contaminatedareas,laboratorychemicals-contaminatedare- as,andindustrialsewagedistrictshigh-throughputscreeningwasperformedthroughstilsuspension mi- cro-culturetechnologytheimpactoftime,temperature,androtationalspeedoffermentationinincubator shaker,andtheph,carbonsource,nitrogensource,andotherphysicalandchemicalfactorsoffermentation mediaontheinhibitoryefectofantagonisticactinomyceteswereinvestigatedtheoptimumconditionof antagonisticactinomycetesfermentationinincubatorwasalsoexploredtheresultsindicatedthatthefer- mentationproductsofantagonisticactinomyceteshadinhibitoryefectoncalbicans,staphylococcusau- reusandothermicrobes;theoptimumconditionofantagonisticactinomycetesfermentationwasdetermined tobefermentatedat29 and220r/minfor96h,withtheph ofthefermentation medium adjustedto 71,andwithstarchandcornsyrupasthebestcarbonsourceandnitrogensource,respectively Keywords:antagonisticactinomycetes;screening;Candidaalbicans;physicalandchemicalfactors :2013-03-26; :2013-05-20 : (0760621234) : (1990- ),,, : (1967-),,,, E-mail:zhaobaohua86178@sohucom

398, 2 [1], AIDS,, 404% 376%, 52 24%,33%,41% [2] 52,,,,,,, [3],, 1 11 实 验 材 料 111 752 ( ),JY92 Ⅱ ( ),MHA ( ),Baird Parker ( ) 112 72, 3 (Moniliaalbican Candidaalbicans) (Staphyloccocusau- reus) (Bacilussubtilis), 113 :1L 25g,40g,08g (NH 4 ) 2SO 4,3gCaCO 3,2gNaCl,pH70~72 : 20g,KNO 31g,K 2HPO 405g,MgSO 4 7H 2O05g,NaCl05g,FeSO 4 7H 2O001g, 20g,pH74~76 :1L 3g, 10g,NaCl5g,pH 74~76YPD :1L 10g,20g,20g 12 实 验 方 法 121, [4] ( 1),, 24, 2mL 30 4d, 12000r/min 5min, 122 MHA YPD, YPD 12h,,, 10 6 CFU/mL, 1 5μL, 3~5 MHA, min, 37 12~24h 123, ph [5-6] ( 1),

399 1 /h 12,24,36,48,60,72,96,120 / 26,265,27,275,28,285,29,295,30,305,31,315,32,325 /(r min -1 ) 150,160,170,180,190,200,220 ph 68,69,70,71,72,73,74,,,, + ( 1 1), + ( 1 1), + ( 1 1),,,NaNO3,NH4NO3 124, 20g/L (D 600nm=06~08), 50mL 250mL,37, 180r/min 1 750μL, 2 500μL, 3, 1h 1, D 600nm 2 21 静 止 悬 浮 微 培 养 技 术 筛 选 拮 抗 放 线 菌 的 效 果,, ( 2) 22 拮 抗 放 线 菌 的 筛 选 2 a ;b3d 72, 2 (7 10 ), 5,10mm, 10 :, ( 3a);, ( 3b); ( 2) a ;b 3 10

400 2 23 10 号 菌 株 抑 菌 活 性 检 测 结 果, 10, 4 24 理 化 因 子 对 抑 菌 效 果 的 影 响 a ;b 4 10, 5, 96h 29 220r/min ph71 5 10

401 25 发 酵 产 物 对 靶 试 菌 的 抑 制 效 果 2~4h, ; 2~4h, ; 4~6h,, 6 a ;b ;c 6 3,,, [7-8] 72, 2, 3 [9] ph, 3, 10, : [1], [J],2010,35(11):873-876 [2],, 225 [J],2003,13(4):380-381 [3], [M] :,2004:102-321 [4],,, :CN,101709268A [P]2009-12-23 [5],,, [J],2005,33(1):59-63 [6],, [J],2004,24(4):12-14 [7],, [J],2007,9(32):513-519 [8],, [J],2005,24(2):26-31 [9],,, [J],2006,25(1): 11-15 ( )