B IO TECHNOLOGY BULL ETIN K562 DNA 1 1, 2 1 1 2 ( 1, 710062; 2, 710062) : UVB UVC DNA,, DNA [ 1 ],, UVB, UVA K562 DNA, UVB K562 DNA /, DNA, DNA UVC K562 DNA UVB UVC DNA, ;,, : UVB UVC DNA DNA Observation of the DNA Extracted from K562 Cells Exposed in Ultraviolet L ight by Atom ic Force M icroscopy L iu Ying 1 Q i Hao 1, 2 Sun Runguang 1 Chen W enfang 1 Guo W ei 2 ( 1 Shanxi N orm al University, Departm ent of Physics, B iophysics Laboratory, X i an 710062; 2 Shanxi N orm al University, Departm ent of B iology, X i an 710062) Abstrac t: The partner of the laboratory that the author stayed use the comet assay detect UVB, UVC radiation cause the broken of DNA in a short period. But after a long time the comet assay could not detect the phenomenon, we suppose that results was due to the DNA molecules cross2linked. In order to exp lain this phenomenon much more directly, and also give a correct model of DNA observa2 tion, DNA from the K562 cells was extracted that exposed in the UVB, UVC. The result indicatd that DNA damage increase as the dose of UVB, UVC increasing. In a short time, some of DNA of K562 cells exposed in UVB appeared to break to fracture. After a long time, majority of DNA appeaed to be cross2linking. UVB affected on DNA was much greater than UVB. Majority of DNA of K562 cells exposed in UVC for a short time appear to break and cross2link. Thus, it p roves that long time exposure cause DNA molecules appear to winding and condensation, with other DNA damage. Key wo rds: A tom ic force m icroscopy UVB UVC B reak of DNA Cross2link of DNA (AFM ), DNA [ 2 9 ] 1992, Bustamante [ 10 ] DNA, DNA, DNA,, AFM DNA [ 11 13 ],, Peter [ 14 ] AFM DNA : 2008212215 : (2006C 2 34) : (19812),,,, ; E2mail: 8110131846@ sina. com : (19582),,,,, ; E2mail: sunrun@pub. xaonline. com
142 B iotechnology B u lletin DNA DNA, DNA DNA [ 15 ] APS2, DNA, AFM [ 16 ] DNA, 3 DNA, DNA AFM,, [ 17 ] AFM DNA,, DNA [ 18 ] DNA, DNA, 1 111 11111 ( ), : SPM 29500J3 (W ET CN II), Si3N4 AFM, 200 m, 0. 12 N /m; (N ikon ), : N ikon TE20002S; : Eppendorf ( 5417C) ; ; UV : ; ZSZ 20, 20 W, 11112 K562 RPM I 1640 + 10% ( ) + 100 IU ( ) ; Hepa126 DMEM + 10% ( ) + 100 IU ( ) ; 80%W il2 liam s E + 20% ( ) + 100 IU ( ) + (2. 5 g/m l) + (0. 4 g/m l) + 2 mmol/l L2, 5% CO 2 37 ; 3 5 11113 DNA, 112 11211 DNA K562, DNA DNA, 11212 DNA DNA : 10 0. 33 0. 03 g/m l, 10 l DNA,,,,,, contact, 1. 0 1. 5 Hz 11213 UVB UVC K562 UVB, UVC, 40 W / cm 2, 25 s, 100 s, 5 m in,, DNA DNA, DNA 30 ng/ m l, 2 211 DNA AFM 12A 12B 12C 10 g/ m l, 0. 33 g/m l, 0. 03 g/m l DNA AFM DNA 3 : 10 g/m l, DNA, AFM ( 12 A) ; 0. 33 g/m l, DNA ( 12B ) ; 0. 03 g/m l, DNA ( 12C) 212 AFM UV DNA 21211 UVB K562 DNA 2 3 K562 UVB 25 s, DNA, ( 22B, 22C) ; UVB 100 s, DNA ( 22C), DNA DNA ( 32C) ; UVB 5 m in,,
: K562 DNA 143 DNA ( 22D ), DNA ( 32D) 21212 UVC K562, DNA 4 5 K562 UVC 25 s, DNA, ( 42B, 52B ) ; UVC 100 s, DNA, DNA ( 42C, 52C),, ( 52C) ; UVC 5 m in,,dna, ( 42D), DNA, ( 52D), DNA A. DNA 10 g/m l; B. DNA 0. 33 g/m l; C. DNA 0. 03 g/m l 1 D NA AFM ( : 5 m 5 m ) A. ; B. UVB 25 s; C. UVB 100 s; D. UVB 5 m in 2 UVB K562 D NA AFM ( : 15 m 15 m ) A. ; B. UVB 25 s; C. UVB 100 s; D. UVB 5 m in 3 UVB K562 D NA AFM ( : 3. 75 m 3. 75 m )
144 B iotechnology B u lletin A. ; B. UVC 25 s; C. UVC 100 s; D. UVC 5 m in 4 UVC K562 D NA AFM ( : 15 m 15 m ) A. ; B. UVC 25 s; C. UVC 100 s; D. UVC 5 m in 5 UVC K562 D NA AFM 3 ( : 3. 75 m 3. 75 m ) AFM DNA, DNA 0. 03 g/m l ( 12C) DNA, DNA DNA UVB, (100 s ), DNA, DNA, DNA AFM DNA, UVB K562 DNA /, DNA UVB,, AFM UVB DNA, UVB DNA / UVC UVB,, UVC DNA UVB UVC 100 s, AFM DNA, 100 s DNA 5 m in UVC, AFM DNA, DNA, 52C, DNA, DNA, UV DNA,UVB DNA, DNA ; UVC, DNA,,UVC UVB, UV 4 DNA, DNA, 0. 03 g/m l DNA, DNA UVB K562 DNA /
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