Microsoft PowerPoint Tagged protein-1 [相容模式]

Ho t 金萬林企業股份有限公司 Http://www.kimforest.com Tagged purification 完整 His, GST, MBP, Strip(ll) 系統套組抗體純化生醫年會特別企畫蛋白純化領導品牌給您完整分生連結到蛋白質純化最優越的系統 1. 凡購買本系列產品享有任選三項加送一項之優惠 2. 凡購買本系列產品可享加價購得以下產品 : ( 加價購品項不得合併滿額贈 ) A. Ultrospec 10 pro 一台 B. Peristaltic Pump P-1 一台加購金額為 $25000 ( 原價 40000) 加購金額為 $5000 ( 原價 83000) Ultrospec 10 pro 細胞濃度測定儀可擕式袖珍細胞濃度測定儀, 特別適用於測定大腸桿菌和酵母可以放在培養箱, 無菌操作臺等使用具可充電電池, 充電一次可用一個月以上偵測區可滅菌, 消毒 OD600 濃度檢測最多可一次列印出 50 個檢測結果 C. Microfuge 16 一台加購金額為 $40000 ( 原價 55000) Microfuge 16 (Beckman) 桌上型微量離心機 Volume range 100-500μl 400μl to 2 ml 2-6 ml 5-20 ml Vivaspin 500 Vivaspin 2 Vivaspin 6 Vivaspin 20 附 24 孔 1.5/2 ml 轉子有各式轉接頭可選購離心機規格 : Max g-force:16,163 g Max speed: 14,800 rpm 採用無炭刷式馬達 HWD:17.6x22.6x26.6 cm E. Vivaspin 濃縮管柱全新系列產品可享特別加購價優惠 : 3 000 28-9322-18 28-9322-40 28-9322-93 28-9323-58 5 000 28-9322-23 28-9322-45 28-9322-94 28-9323-59 P-1 Pump 精準純化幫浦系統來電訂購既送來電禮! 訂購專線 :0800-009695 活動時間即日起 ~6/26 有任何問題請洽當區業務或來電詢問 Capture buffer 1. 溶解 DNA gel 2. 轉到 GFX 3.Wash Column 4.EluteDNA Column 操作簡易, 快速純化 : 15 分鐘完成 DNA band purification ( 自溶液只要 5 分鐘 ) 品質保證 : 回收率及純度高, >80% from solution, >60% from gels. 可去除 PCR 反應中, 99.5% 的 PCR primer & free nucleotides 10 000 28-9322-25 28-9322-47 28-9322-96 28-9323-60 純化 DNA, 快速又便捷 DNA gel MWCO value 30 000 28-9322-35 28-9322-48 28-9323-17 28-9323-61 60 0 C 50 000 28-9322-36 28-9322-57 28-9323-18 28-9323-62 100 000 28-9322-37 28-9322-58 28-9323-19 28-9323-63 25 25 25 12 3516 5514 7323 5224 驚爆 7 折可搭配 GE 專屬 Trap column 使用, 相當便利流速範圍 : 0.1-8 ml/min 轉盤非對稱性設計將正向流動的脈衝減至最低可配合多種不同的套管材質以耐受不同之化學溶劑遙控裝置, 可與紫外可見光檢測器以及分液收集器搭配, 達到同步監測樣品及收集之採用步進馬達簡單準確有不同口徑 1mm, 2.1mm, 3.1mm 的管線, 以達不同流速之要求壓力轉盤適合三種管徑的管線無須另行調整當外蓋合上時壓力轉盤會自動調整成正確壓力 D. GFX PCR & Gel Band Purification kit 限購一組 100rxns 加購金額為 $3800 ( 原價 53) 加購價 1800 2800 3600 2700 滿額贈滿兩萬贈凡購買本系列產品, 單筆金額滿兩萬或以上既可享有以下滿額贈凡購買本系列產品, 單筆金額滿伍萬即可抽 ipad 2 及 iphone4 滿兩萬既可任選以下 A.B.C,D 四選一滿伍萬贈滿伍萬既可選擇以下 A.B.C 三者 A. 電影票二張 A. 陶版屋二客 B. Pizza 卷二張 B. 離心過濾濃縮管柱 Vivaspin500 columns 一盒 C. 500 元金萬林商品折價券請選擇其一過濾膜孔徑 :3kD,5kD,10kD) D. 7-11 禮券二張 C. 2000 元金萬林商品折價券

His-Tagged Protein Purification Selection Guide Vector Expression Cell lysis Purification Precharged Ni Sepharose media 有無 automated puification system 如 : AKTA System 有系統高解析度可大量純化可擁有高流速無系統 Contains Ni Sepharose High Performance Contains Ni Sepharose 6 Fast Flow Contains IMAC Sepharose High Performance Contains IMAC Sepharose 6 Fast Flow Uncharged IMAC Sepharose media 有無 automated puification system 如 : AKTA 可自行依 sample 特性 System 接上 Cu2+ Zn2+ Co2+ +Fe2+ 等不同離子有系統無系統高解析度可大量純化可擁有高流速 His GraviTrap columns Pre-charged Ni Sepharose Binding capacity 17-5268-01/-02 Ni Sepharose HP 34 ~ 40 mg( His) 6-25 / 100 ml 13992 / 51304 tagged /ml 17-5318-06 /-01/-02 Ni Sepharose 6 FF gel 5 /25/100ml 3148 / 11427 / 43142 17-5247-01/-5248-02 HisTrap HP 34 5x1/5x5ml 95 / 34980 17-5319-01/-5255-01 HisTrap FF ~ 40/200 mg Histagged 5x1/5x5ml 7579 / 31482 11-0004-58/-5286-01 HisTrap FF crude 5x1/5x5ml 7812 / 33814 HiPrep columns 28-9365-51 HisPrep FF 16/10 His GraviTrap columns 800 mg His-tagged 20 ml 21780 11-0033-99 His GraviTrap 28-4013-51 His GraviTrap Kit ~ 40 mg His-tagged 10x 1 ml 9838 20x1ml HisGraviTrap 及 1 組 His Buffer Kit 20988 His MultiTrap 96-well filter plate 28-4009-89 His MultiTrap HP 34 1000μg/well 4 x 96-well plates 37419 28-4009- His MultiTrap FF 800μg/well 4 x 96-well plates 37419 His Spin columns 28-4013-53 His SpinTrap 34 ~750μg His-tagged 50x100 ul 16016

不需離心, 節省時間離心, 取上清液樣品 Screening 少量純化微量純化 HisTrap HP Ni Sepharose High Performance Ni Sepharose 6 Fast Flow HisTrap FF + His SpinTrap Empty Columns C column MultiTrap 96-well Filter Plates 不需離心, 節省時間離心, 取上清液 Ni Sepharose Empty Columns 6 Fast Flow + PD-10 or Micro Spin columns Syringe 針筒進行純化 His MultiTrap 96-well Filter Plates 大量純化 >200 mg 少量純化 His MultiTrap HP His MultiTrap FF Gravity-flow 重力純化 HisTrap FF Crude HisPrep FF 16/10 HisTrap FF HisTrap Column FPLC 系統或針筒進行純化 HisTrap FF Crude His GraviTrap column HisTrap IMAC HP IMAC Sepharose High Performance IMAC Sepharose 6 Fast Flow HisTrap IMAC FF HisTrap IMAC FF + Empty Columns C column HisPrep IMAC FF16/10 Un-charged IMAC Sepharose Binding capacity 17-0920-06 /-07 IMAC Sepharose HP 34 ~ 40 mg(ni 2+ ) 25/100 ml 13992 / 43142 25 mg(cu 2+ ) 17-0921-07/-08 IMAC Sepharose 6 FF 15 mg(zn 2+ ) 25/100 ml 9794 / 32648 17-0575-01 Chelating Sepharose 6 FF -tagged /ml gel 50 ml 13992 Cu 2+,Zn 2+, Co 2+,Ni 2+ 17-0920-03/-0920-05 HisTrap IMAC HP ~ 40/200 mg(ni 34 2+ ) 5x1/5x5ml 7299 / 24486 25/125 mg(cu 2+ ) 17-0408-01/-0409-03 HisTrap Chelating HP 15//75 mg(zn 2+ ) 5x1/5x5ml 5947/ 23320 -tagged /ml gel 17-0921-02/-0921-04 HisTrap IMAC FF 5x1/5x5ml 5480 / 19822 HiPrep columns 28-9365-52 HisPrep IMAC FF 16/10 Empty columns ~ 800 mg(ni 2+ ) 500 mg(cu 2+ ) 300 mg(zn 2+ ) -tagged /ml gel 20 ml 16940 體積 PD-10 & reservoir MicroSpin column 17-0435-01 Empty PD-10 columns 13 ml 50 6635 18-3216-03 LabMate PD-10 buffer reservoir 25 ml 10 1283 27-3565-01 Micro Spin columns 650 μl 100 13464 His Buffer Kit 11-0034-00 His Buffer Kit Anti-His Antibody 2x100ml phosphate buffer (8xstock,pH7.4) 1x100ml (2M imidazole) 4781 27-4710-01 Anti-His Antibody 700 ul(2.6ug/ul) 8470

GST-Tagged Protein Purification Selection Guide 高解析度 Purification 有系統可大量純化, 可擁有高流速 Vector Expression Cell lysis Glutathione Sepharose media 有無 automated purification system 如 : AKTA System E.Coli BL21 無系統樣品 Screening 微量純化 Contains Glutathione Sepharose High Performance Contains Glutathione Sepharose 4 Fast Flow Contains Glutathione Sepharose 4B Glutathione Sepharose Cleavage enzymes Cleavage site Factor Xa protease: Mr 28~30 kd Thrombin protease: Mr 37 kd PreScission Protease: Mr 46 kd Syringe 針筒進行純化 Gravity-flow 重力純化 Expression Purification Cleavage Enzymes Enzymatic cleavage Expression - pgex Vectors products Removal of glutathione &protease Coding region for Enzymes cleavage Detection E.Coli 27-1542-01 E. Coli BL21 1 vial 4070 Glutathione S-transferase Gene Fusion Vectors 27-4805-01 pgex-1lt EcoRI/BAP Thrombin protease 918-935 5ug 22000 27-4801-01/-4587-01 pgex-2t / pgex-2tk Thrombin protease 918-935 25ug 22000 27-4580-/-4581/-4583-01 pgex-4t-1 /-2 /-3 Thrombin protease 918-935 25ug 20000 27-4803-01 pgex-3x Factor Xa Protease 921-935 25ug 22000 27-4584-/-4585-/-4586-01 pgex-5x-1 /-2 /-3 Factor Xa Protease 921-932 25ug 22000 27-4597-/-4598-/-4599-01 pgex-6p-1 /-2 /-3 PreScission Protease 918-938 25ug 22000 Sequencing Primer 27-1410-01 pgex 5 Sequencing Primer 5 -d[gggctggcaagccacgtttggtg]-3 0.05A 260 unit 7370 27-1411-01 pgex 5 Sequencing Primer 5 -d[ccgggagctgcatgtgtcagagg]-3 0.05A 260 unit 7370 Enzymatic cleavage 27-0846-01 Thrombin protease 500 units 3608 27-0849-01 Factor Xa Protease 400 units 20275 27-0843-01 PreScission Protease 500 units 9747 Detection - Anti-GST Antibody 27-4577-01 Anti-GST antibody 0.5ml,50 detections 11644 RPN1236 Anti-GST HRP Conjugate 75 ul 10098 RPN1237 ECL GST Western Blotting Detection Kit(Anti-GST HRP conjugate, 75 µl; blocking reagent, 20 g.positive recombinant GST control, 10 µl.ecl Detection Reagents 1 and 2, 125 ml each) 1 Kit 17952 27-4592-01 GST 96-Well Detection Module 5 plates 22154 27-4591-01 GST Detection Module 50 detections 15158

GSTrap HP Glutathione Sepharose High Performance 大量純化,>50 mg GSTPrep FF 16/10 Glutathione Sepharose 4 Fast Flow 少量純化,<50 mg GSTrap FF GSTrap 4B GSTrap Column Glutathione Sepharose 4 Fast Flow Glutathione Sepharose 4 B GST MultiTrap FF MultiTrap 96-well Filter Plates GST MultiTrap 4B 離心 GST SpinTrap Purification Module FPLC 系統或針筒進行純化 GSTrap FF GSTrap 4B RediPack GST Purification Module Bulk GST Purification Module Purification - Glutathione Sepharose Binding capacity 17-5279-01/-02 Glutathione Sepharose HP 34 ~ 10 mg recombinant GST/ml medium 25 / 100ml 30316/1100770 17-5132-01/-02 Glutathione Sepharose 4 FF ~ 10 mg recombinant GST/ml medium 25 / 100 ml 27984/97944 17-0756-01 Glutathione Sepharose 4B ~ 5 mg recombinant GST/ml medium 25 ml 10811 17-5281-01/-02 GSTrap HP 34 ~ 10 / 50 recombinant GST 5x1/5x5ml 16324/52470 17-5130-01/-5131-02 GSTrap FF ~ 10 / 50 recombinant GST 5x1/5x5ml 11310/47806 28-4017-45/-48 GSTrap 4B ~ 5 / 25 recombinant GST 5x1/5x5ml 15158/54802 HiPrep columns 28-9365-50 GSTPrep FF16/10 200 mg recombinant GST 20 ml 32780 GraviTrap columns 27-4570-02 RediPack GST Purification Module ~ 5 mg recombinant GST/ml 2 x 2 ml 22154 27-4570-01 Bulk GST Purification module ~ 5 mg recombinant GST/ml 10 ml (Kit) 22389 Spin columns 27-4570-03 GST SpinTrap purification Modules 400μg GST-tagged 50 columns 18656 GST MultiTrap 96-well filter plate 28-4055-01 GST MultiTrap FF 0.5 mg GST-tagged x96-well 4 plates 38478 28-4055-00 GST MultiTrap 4B 0.5 mg house liver,gst x96-well 4 plates 38478 Removal of glutathion & proteases Desalting, Benzamidine(high sub) Binding capacity 管柱 17-0851-01 PD-10 Desalting columns 85~260 1.0~2.5 ml 30 x 8.3 ml 10144 Gravity 17-1408-01 HiTrap Desalting <1.5 ml 5x5ml 7812 HiTrap column Affinity column 去除 Serine protease 17-5123-10 Benzamidine Sepharose 4 FF (high sub) >35 mg/ml 25 ml 11660 XK or Spin column 17-5143-01/-5144-01 HiTrap Benzamidine FF >35/175 mg trypsin 5x1/1x5ml 11077 / 10494 HiTrap column

Strep-tag II s & MBP-tagged s StrepTactin Sepharose Strep(II)-technology -Strep(II)-tag, 8 aa, 1 kd (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) -Often no cleavage of tag is necessary -Placement of tag both N-or C-terminal -StrepTactin 對 Strep (II)-tag 的結合效能約為 Streptavidin 的 100 倍 - 高結合量 : ~ 6 mg/ml medium Regeneration of StrepTactin Sepharose 0.5 M NaOH, 14 min GE 他牌 2 mm HABA, 22 min -Strep (II)-tag 之應用目的分子量小不影響蛋白質結構不需切除節省實驗時間高專一性結合, 可結合 His-tag 純化進行 Dualtag 純化技術, 產生超高專一度及高純度產物 - 省時 - 容易操作 - 以 NaOH 再生 ; 步驟簡單 - 經濟耐用 Dextrin Sepharose MBP-technology -MBP (maltose binding )-tag, 42.5 kd - 可用專一性酵素切除 Ex: Factor Xa, Thrombin protease -Expression in E. coli - 純化帶有 maltose binding 之重組蛋質 - 高結合量 : 7~ 16 mg/ml medium -MBP-tag 之應用目的提高表現量增加重組蛋白質的溶解度協助蛋白質正確 folding Easy scale-up Dextrin Sepharose HP MBPTrap HP 1 ml Dextrin Sepharose HP XK 26/20 Strep Tactin / Dextrin Sepharose Binding capacity 28-9355-99/-00 StrepTactin Sepharose HP ~ 6 mg/ml 10/50 ml 18656/80454 34 28-9355-97/-98 Dextrin Sepharose HP 7~16 mg/ml 25/100 ml 23320/73458 28-75-46/-48 StrepTrap HP ~6/30 mg Strep(II)-tagged 5x1/5x5ml 32648 / 85118 34 28-9187-78/-80 MBPTrap HP ~10/50 mg Strep(II)-tagged 5x1/5x5ml 12826 / 50139 金萬林企業股份有限公司台北 / 新竹 / 台中 / 台南 / 高雄 / 台北市忠孝東路五段 510 號 15 樓免付費專線 :0800-009-695 Mail:Kimforest@gmail.com TEL:02-27260768 FAX:02-27275689