165 DOI:10.13350/j.cjpb.190209 HQR)OST + 1,2,c 2,3,c 1,3, 1,3,! " 1,3 (1. /,-+, / V 810001;2. /,-+ %&';3. / ( #$%&') C2 34 7 DE" X FG(EmTspan)? @ NM NCBI EmTspanFG?X H I, 2 ProtParam ProtScale SOSUI DNASTAR SignalP Cel PLoc SOMPA NetPhos MotifScan Phyre2 TMHMM E 3456 7FGI?H BI Y6[ M X D ; M$ N O @, 2 ABCpred IEDB SYFPEITHI5 6 7 8 9 B 6 [ T 6 [? N M,M MEGA X 56@ Tspan? Q EmTspanFG 236 X HI, ^S C1163H1853N291 O315S21,] S 32.77,S F G; 4 X, M 6 [ ;N O @,α c 46.19%, β 20.34%, β 8.05%, 25.42%;EmTspanFG V? B 6 [ TCL 6 [ Th6 [? N M S7 15 8 ; Q 7 DE "? TspanD \ P '? TspanO a 3 4 8 9 EmTspanFG,- -? B6[ T 6 [ N M, B, S D E "? ( U V E @ A H > DE"; X FG; 34; NM R383.33!"#$ A %& 1673 5234(2019)02 0165 06 [.2019Feb;14(2):165-170,177.] 犅犻狅犻狀犳狅狉犿犪狋犻犮犪狀犪犾狔狊犻狊狅犳狋犲狋狉犪狊狆犪狀犻狀狊犳狉狅犿犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 YANG Yu 1,2,ZHANG Yao gang 2,3,ZHANGLing qiang 1,3,REN Li 1,3,FAN Hai ning 1,3 (1. 犙犻狀犵 犺犪犻犝狀犻狏犲狉狊犻狋狔犎狅狊狆犻狋犪犾, 犡犻狀犻狀犵, 犆犺犻狀犪 810001;2. 犔犪犫狅狉犪狋狅狉狔, 犙犻狀犵犺犪犻犝狀犻狏犲狉狊犻狋狔犎狅狊狆犻狋犪犾 ;3. 犓犲狔犔犪犫狅狉犪狋狅狉狔狅犳犈犮犺犻狀狅犮狅犮犮狌狊犻狀犙犻狀犵犺犪犻犘狉狅狏犻狀犮犲 ) 犃犫狊狋狉犪犮狋 犗犫犼犲犮狋犻狏犲 Tousebioinformaticsoftwaretopredictandanalyzethestructureandepitopesoftetraspanin proteinsfrom 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 (EmTspan). 犕犲狋犺狅犱狊 AminoacidsequencesofEmTspanproteins were downloadedfromthencbidatabase.protparam,protscale,sosui,dnastar,signalp,cel PLoc,SOMPA,Net Phos,MotifScan,Phyre2,TMHMM,andotherbioinformaticsoftwarewereusedtopredictandanalyzethephysico chemicalproperties,subcelularlocalization,transmembraneregions,phosphorylationandpost translationalmodification sites,andsecondaryandtertiarystructuresoftheprotein.b andt celepitopeswereanalyzedandpredictedusingthe softwareabcpred,iedb,andsyfpeithi.a molecularevolutionarytreewasconstructedfortspanusing MEGA X. 犚犲狊狌犾狋狊 EmTspanproteinconsistedof236aminoacids,itsmolecularformulawasC1163H1853N291O315S21,anditsinsta bilityindexwas32.77,soitisastableprotein.theproteinhasfourtransmembraneregionslocatedinthecelmem brane.α helicesaccountfor46.19% oftheprotein'ssecondarystructure, β sheetsaccountfor20.34%, β turnsaccount for8.05%,andrandomcoilsaccountfor25.42%.emtspanproteincontainsmanyphosphorylationandpost translation almodificationsites.theproteinhas7b celepitopes,15ctl celepitopes,and8th celepitopes.thetspanof 犈. 犿狌犾狋犻犾狅犮狌犾犪狉犻狊 iscloselyrelatedtothetspanofhymenolepismicrostoma. 犆狅狀犮犾狌狊犻狅狀 Accordingtobioinformatics, EmTspanproteinispredictedtohaveseveralB andt celepitopesandgoodantigenicity.thesefindingscanprovidea theoreticalbasisforimmunodiagnosisofechinococcosisandthedevelopmentofvaccineagainst 犈. 犿狌犾狋犻犾狅犮狌犾犪狉犻狊. 犓犲狔狑狅狉犱狊 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 ;tetraspanin;bioinformatics;epitope % D E " ( 犈犮犺犻狀狅犮狅犮犮狌狊 犿狌犾狋犻犾狅犮狌犾犪狉犻狊 )?- >R?% K [1]?>R Y V W / UE [2], "#!N(? `? [3],b %? ^[4], ;, U b,^ ' 2017 # $ ( " ( " (No.2017YFC0909900); / 2 ( (No. 2014ZJ719); / (No.2016 wjzd 04) D,E mail:renliweimin_xn@126.com () P (1992- ),, %,- \ ( ( ScFG E mail:2296651604@qq.com
166 8?HI, D E" /, J [5-7], $,?, 2 34 7?, b [8] ( 2 34 5 6 8 9 7 D E " X F G ( 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 tetraspanin,emtspan)?h BI,N O @ B 6[ T 6[ NM E,S%??( @AH> *+, 1 犈犿犜狊狆犪狀 2'-./[;'./ NCBI K DE" Tspan? HIDX HI,FG J S LN902404.1 D CDS43046. 2 2 犈犿犜狊狆犪狀 201 2 EXPASY(htps://www.expasy.org/)? - 756 ProtParam(htps://web.expasy.org/ protparam/) 7 EmTspanFG?H BI( ^ I T M E _ $ E );C 2 ProtScale (htps://web.expasy.org/protscale/)5 6 7 EmTspanFG?O B ; 2 SOSUI(htp:// harrier.nagahama i bio.ac.jp/sosui/)8 9 EmTspan FG? ; 2 DNASTAR 5 6 7 EmTs panfg?o B bb ab? N ( B a b? ;C 2 SignalP4.1 Server(htp://www.cbs.dtu.dk/services/SignalP/) - _ H 8 9 7 EmTspan F G? 3 J K; 2 Cel PLoc2.0,Euk mploc2.0(htp://www.csbio. sjtu.edu.cn/bioinf/euk multi 2/)_H 7 EmTspan FG?Y6[ M;M SOMPA(htps://npsa pra bi.ibcp.fr/cgi bin/npsa_automat.pl?page=npsa_ sopma.html)56 7 EmTspanFG?NO @; 2 NetPhos3.1 Server(htp://www.cbs.dtu.dk/ services/netphos/)8 9 EmTspan F G? M $;C 2 MotifScan(htps://myhits.isb sib.ch/cgi bin/motif_scan) 7 EmTspan ;? M $; C 2 ABCpred(htp://crdd.osdd.net/raghava/ab cpred/)diedb,mini(htp://tools.iedb.org/bcel/) NM89561 89 7 EmTspanFG? B 6[ NM; 2 SYFPEITHI(htp://www.syf peithi.de/bin/mhcserver.dl/epitopeprediction.htm) 7 HLA A 02 01 D HLA DRB 0401 M $? T 6[NM; 2 Phyre2(htp://www.sbg.bio. ic.ac.uk/phyre2/html/page.cgi?id=index)- _ H@ FG? O @ ; 2 TMHMM Serverv. 2.0(htp://www.cbs.dtu.dk/services/TMHMM/) 56 EmTspanFG?X QR89; NCBI 6 E " ( 犈犮犺犻狀狅犮狅犮犮狌狊犵狉犪狀狌犾狅狊狌狊, CDS15929.1) L M ; ( 犆犪犲狀狅狉犺犪犫犱犻狋犻狊犲犾犲 犵犪狀狊,CTQ87024.1) \ P ' ( 犎狔犿犲狀狅犾犲狆犻狊 犿犻犮狉狅狊狋狅犿犪,CUU97506.1) ;1' ( 犜犪犲狀犻犪狊狅犾犻 狌犿,AQQ11629.1) c ` ( 犉犪狊犮犻狅犾犪犺犲狆犪狋犻犮犪, PIS91274.1)D % N ` ( 犃狀犮狔犾狅狊狋狅犿犪犱狌狅犱犲 狀犪犾犲,KIH49009.1)Tspan X H I, 2 MEGA X 56@ Q,QR) B 7 1 犈犿犜狊狆犪狀 2;'./ M NCBI K, EmTspan F G? S EmuJ_001077400(LN902404.1), 3967bp, V6,5 V, 236 X H I EmTspan(CDS43046.2)?X HIS:mvhftnpdn srvivtrrmalscggkclkflv fnaivfigggivagygvflivktkaags vsvtlpafitfglfligflgcfgacynntcmlktfavivailvaeivcgiv lvyrheemqreikdltahgrnasdplksiyklqeeleccggvgptdws kpypasccksgkenctqpyqqgcavamyeqikdsslafglilivcliqig avicacclakkvneyekv 2 犈犿犜狊狆犪狀 289:3 M ProtParam 56 7 EmTspanFG? ^S C 1163H 1853N 291O 315S 21 ; ITS25.62553 10 3,H E _ $ (TheoreticalpI)S 8.44; : 30h(mammalian),>20h(yeast),>10h(Escherichi a);] 32.77,S F G;8 B S 109.45,Z[ S18420 3 犈犿犜狊狆犪狀 2 ( ) : 2 ProtScale 5 6 7 EmTspan F G? O ( ) B, 1 EmTspan F G N, O SOSUI 7 EmTspanFG? S0.630, S FG 4 犈犿犜狊狆犪狀 2 :[ :ca6 [ 6: 2 DNASTAR 5 6 7 EmTspaF G? O B S:3-14,57-61,89-96,121-143,149-156,162-189,196-201;bB S:5-11,23-25, 42-43,55-61,125-132,137-146,151-154,160-170,176-189,197-202; a b? :5-14,16-19,22-26,55-62,89-94,122-145,149-189,196-203,226-236;N( Bab? S:5-10,55-58,121-131,136-141,147-153,164-170,177-187,196-201,229-236( 2)
167 `: O NX?M, O NO( ) B(7 N B, NO B) 1 犈犿犜狊狆犪狀 2 ( ) : Notes:Thehorizontalaxisstandsforthepositionofaminoacidsinthisproteinandverticalaxisstandsforthehydrophicity.Thepositivevalue ofverticalaxisrepresenthydrophobicityandthenegativevaluerepresenthydrophilicity. 犉犻犵.1 犎狔犱狉狅狆犺犻犾犻犮犪狀犱犺狔犱狉狅狆犺狅犫犻犮犿犪狆狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 2 犈犿犜狊狆犪狀 2 :[ :ca6 [ 6:<= 犉犻犵.2 犎狔犱狉狅狆犺犻犾犻犮, 犳犾犲狓犻犫犾犲狉犲犵犻狅狀, 犪狀狋犻犵犲狀犻犮犻狀犱犲狓犪狀犱狊狌狉犳犪犮犲狆狉狅犫犪犫犻犾犻狋狔狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 5 犈犿犜狊狆犪狀 2 B 6CDE SigalP 7 EmTspan F G a 3 J K, S X FG( 3) M Cel PLoc2.0D Euk mploc2.0 7 EmTspanFG M 6[ 25.42%( 4) FG V Tab, / 4 犈犿犜狊狆犪狀 2 F@<= 犉犻犵.4 犛犲犮狅狀犱犪狉狔狊狋狉狌犮狋狌狉犲狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 3 犈犿犜狊狆犪狀 2 B 犉犻犵.3 犛犻犵狀犪犾狆犲狆狋犻犱犲狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀犪狀犪犾狔狊犲犱 6 犈犿犜狊狆犪狀 2 F@ M SOMPA 7 EmTspanFG?NO @,α c 46.19%, β 20.34%, β 8.05%, 7 犈犿犜狊狆犪狀 2 9[ M NetPhos3.1Server 7,EmTspanFGV 8 X M $ M 21 62 141 167 173 177 201 202MX ;7 X M$ M 15 56 57 66 99 134 183MX ;1 X M $ M 233 M X ( 5) Motif Scan 7,EmTspan F G 13 ; M $, 3 L M$(92-95,139-142, 181-184),2 F G > Ⅱ M $(5-8,177-180),5 N-. M $(42-47,85-90,160-165,189-194,218-223) 3 F G > C M$(15-17,56-58,177-179)
168 5 犈犿犜狊狆犪狀 2 9 <= 犉犻犵.5 犘狉犲犱犻犮狋犻狅狀狅犳狆犺狅狊狆犺狅狉狔犾犪狋犻狅狀狊犻狋犲狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 8 犅 DE: <= ABCpred5689? B 6 [ N M L a b (S S0.80)?HI :3-16(0.87),27-40(0. 82),43-56(0.86),79-93(0.91),89-98(0.82),94-103(0.81),118-131(0.80),130-141(0.82),136-149(0.89),151-164(0.84),168-183(0.84),217-232(0.83);IEDB 5 6 8 9 EmTspan F G? B NMS:5-10,121-131,135-141,147-154,164-170,177-187,194-201 5689 % B FG?O B ab? bb B E1 7, L 7 B 6[ NM(N1) 9 犜 DE <= 2 SYFPEITHI- _H? HLA A 02-01 7,23MS,EmTspanFG 15 CTL 6[NM; 2 SYFPEITHI- _ H? HLA DRB 0401 7, 22 M S,EmTspan F G 8 Th 6 [ N M (N 2) N EmTspan HLA A 02-01,^ / HLA DRB 0401 F H Order 1 犈犿犜狊狆犪狀 2 犅 DE <= 犜犪犫犾犲 1 犘狉犲犱犻犮狋犲犱犅犮犲犾犲狆犻狋狅狆犲狊狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 X M Positionofaminoacid NMHI Sequencesofepitope 1 5-10 TNPDNS 2 121-131 YRHEEMQREIK 3 136-141 HGRNAS 4 147-154 SIYKLQEE 5 164-170 TDWSKPY 6 177-187 SGKENCTQPYQ 7 196-201 EQIKDS X M HJ Positionof Order aminoacid HLA A 02-018? NMHI EpitoperecognizedbyHLA A 02-01 2 犈犿犜狊狆犪狀 2 犜 DE <= 犜犪犫犾犲 2 犘狉犲犱犻犮狋犲犱犜犮犲犾犲狆犻狋狅狆犲狊狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 Score X M HJ Positionof Order aminoacid HLA DRB1 04018? NMHI EpitoperecognizedbyHLA DRB1 0401 1 206-214 GLILIVCL 30 1 48-62 YGVFLIVKTTKAAGS 28 2 202-211 SLAFGLIL 29 2 27-41 LKFLVFFFNAIVFIG 26 3 76-84 LLLFLIGFL 28 3 208-222 ILIVCLIQIGAVIC 26 4 143-151 PLLKSIYKL 26 4 1-15 MVHFTNPDNSRVIVT 22 5 209-217 ILIVCLIQI 26 5 26-40 CLKFLVFFFNAIVFI 22 6 104-112 VAILLVAEI 24 6 45-59 VAGYGVFLIVKTTKA 22 7 147-155 SIYKLQEEL 24 7 97-111 LKTFAVIVAILLVAE 22 8 207-215 LILIVCLI 24 8 192-206 VAMYEQIKDSSLAFG 22 9 19-27 ALSCGGKCL 23 10 44-52 IVAGYGVFL 23 11 72-80 TTFGLLLFL 23 12 101-109 AVIVAILLV 23 13 108-116 LVAEIVCGI 23 14 111-119 EIVCGIVLL 23 15 112-120 IVCGIVLLV 23 Score 10 犈犿犜狊狆犪狀 2KF@6>?cA<= 2 Phyre2 - _H@? EmTspanFG? O @ 6 2 TMHMM 5689 EmTs pan 4 X, N C S:32-54, 67-89,99-121,201-223( 7) 11 RS9@I, M MEGA X @? TspanFG? N JQ 8 DE"? TspanFGD\P '?
169 TspanFG b`),- a, c ` TspanFG) B a 6 犈犿犜狊狆犪狀 2KF@<= 犉犻犵.6 犜犲狉狋犻犪狉狔狊狋狉狌犮狋狌狉犲狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 7 犈犿犜狊狆犪狀 2>?cA<= 犉犻犵.7 犘狉犲犱犻犮狋犻狅狀狅犳狋狉犪狀狊犿犲犿犫狉犪狀犲犻狀犈犿犜狊狆犪狀狆狉狅狋犲犻狀 8 ' 犈犿犜狊狆犪狀 ;'./ RS9 犉犻犵.8 犆狅狀狊狋狉狌犮狋犻狅狀狅犳犿狅犾犲犮狌犾犪狉犲狏狅犾狌狋犻狅狀狋狉犲犲犫犪狊犲犱狅狀犈犿犜狊狆犪狀犪犿犻狀狅犪犮犻犱狊犲狇狌犲狀犮犲 V TspanFG 4) X F G K? % L, N. 6[ [9] ChenE [10-13] E(,TspanFG-?N T,^? E Tspan F G F G 2, 6[N( /D3J [14-15], T 6 [ ",! D E _ Tspan FG- DE" 3 _ I.? 2D] C FGI P S? AFGI 5 )?; QE [16] (, ;?FGIT 3J N 6[ E " ( M NetPhos 3.1 Server 7 EmTspanFGV 8 X M$,7 X M $,1 X M $ Motif Scan 7,EmTspan F G V 13 ; M $, 3 L M$;2 FG >Ⅱ M$;5 N. M$;3 FG > C M$ N EmTspanFG- DE"'? 3 _ #? 2 NM AMB?AJ, M NM R16[? /,
170 R16[, [17] -? _,T 6[? TCR D B 6[? BCR 8 NM /RS B6[NMD T 6[NM B6[NM% 5~15 X ZL B6[? @F6[?],a8?NM M N( [17] αc D β @S, M FG? ",] NM, β D @ T,,- F GN(, NM [18] C2 SOMPA 56 7 EmTspanFG?NO @, β D 8.05% D 25.42% B 6[ NM O B b B a b N ( B a b [19] O B O B X, X X X E?, NM; bb FGI `a?, NO @? ; U? X S B?\,% S ab? -? NM; B FGI X V? B?\, NM? ( 6 ( IEDB ABCpred 5 6 7 89 EmTspanFGV 7 B6[ B NM T 6[ N M % 10~20 X @ B 6[ NM])?,T 6[NM5 @ F6[?] / T 6 [ 8 T 6 [ N M S CTL 6[NM( HLAⅠ 8? NM)D Th6[NM( HLAⅡ 8? NM)? HLAⅠ HLA A 02-01 [20] M SYFPEITHI56? 7 EmTspanFG V 15 HLA A 02-01? N M HLA DRB 0401- HLAⅡ,/ % [21-22] SYFPEITHI56? 7 EmTspanFG V8 HLA DRB 0401? NM X αc β as, FGI @?? # [23] ( 89 EmTspanFG V 4 X, S 7 8 EmTspanFG? @ M $ B "M@A " > 7 EmTspan F G 3 J K, @ EmTspan F G S F G M Cel PLoc 2.0D Euk mploc2.0 7 EmTspanFG M 6 [,@ FG-6[.3J? _ # 2 % & @ " EmTspan? O @, F GIbO @, S"c FGI?AB?( 2M$E@A"H> Q 7 DE"? Tspan F G \ P '? Tspan FGO a 1.,EmTspan DE"? 3 #? 2,^ B 6[ T 6[ N M, B, S DE"??( UVE( @A"H> Z! [1] SchurerJM,GesyKM,ElkinBT,etal. 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌 犾犪狉犻狊 and 犈犮犺犻狀狅犮狅犮犮狌狊犮犪狀犪犱犲狀狊犻狊 inwolvesfrom westerncanada [J].Parasitology,2014,141(2):159-63. [2] TorgersonPR,MacphersonCNL.Thesocioeconomicburdenof parasiticzoonoses:globaltrends[j].vetparasit,2011,182(1): 79-95. [3] JenkinsDJ.WHO/OIEmanualonEchinococcosis,inhumansand animals:apublichealthproblemofglobalconcern[j].intjpar asit,2001,31(14):1717-8. [4] W*,XY, C * H,E.& B & B b c % [J]. A Z 9 :,2011,10 (4):296-8. [5] KouguchiH,MatsumotoJ,KatohY,etal.Thevaccinationpo tentialofemy162antigenagainst 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 in fection[j].biochemlbiophysrescommun,2007,363(4):915-20. [6] V,;. DE' Em18( Q [J].,2009,25(1):67-9. [7] P, V. DE' # Bb EmⅡ/3 Em14-3-3 \ 6 [? ( [J].6 [ 9 :, 2008,24(8):781-4. [8] QinC,SunY,DongY.A New MethodforIdentifyingessential proteinsbasedonnetworktopologypropertiesandproteincom plexes[j].plosone,2016,11(8):e0161042. [9] JiangX,ZhangJ,HuangY.Tetraspaninsincelmigration[J]. CelAdhesMigr,2015,9(5):406-15. [10] ChenL,YuanD,ZhaoR,etal.SuppressionofTSPAN1by RNAinterferenceinhibitsproliferationandinvasionofcoloncanc ercels 犻狀狏犻狋狉狅 [J].TumoriJ,2010,96(5):744-50. [11] HoltersS,AnackerJ,JansenL,etal.Tetraspanin1promotes invasivenessofcervicalcancercels[j].intjoncol,2013,43 (2):503-12. [12] ChenY,Peng W,LuY,etal.MiR 200aenhancesthemigra tionsofa549andsk MES 1celsbyregulatingtheexpressionof TSPAN1[J].JBiosci,2013,38(3):523-32. [13] ChenL,ZhuY,LiH,etal.KnockdownofTSPAN1byRNA silencingandantisensetechniqueinhibitsproliferationandinfiltra tionofhumanskinsquamouscarcinomacels[j].tumorij, 2010,96(2):289-95. [14] Yanez MoM,Mitelbrunn M,Sanchez MadridF.Tetraspanins andintercelularinteractions[j].microcirculation,2001,8(3): 153-68. [15] Levy S,Shoham T.Protein proteininteractionsinthetet raspaninweb[j].physiology,2005,20(4):218-24. [16] Q,,,E.FGI ; ( Q [J]. A4 9:,2014,16(9):989-91. [17]. [M]. Z,2016:1-464. (()177*)
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