Leica CM1850 Leica CM1850 V2.6 C 2012 10 14 0471 86102 C

Leica Biosystems Nussloch GmbH () Leica Biosystems Nussloch GmbH Leica Biosystems Nussloch GmbH Leica Biosystems Nussloch GmbH Heidelberger Str. 17-19 D-69226 Nussloch Germany +49 (62 24) 143-0 +49 (62 24) 143-268 www.leicabiosystems.com Leica C M 1850 3

1....6 1.1... 6 1.2... 6 1.3... 6 1.4... 6 2....7 2.1... 7 2.1.1... 7 2.1.2... 8 2.2... 8 2.3... 9 2.4... 9 2.5... 10 2.6... 10 2.7... 11 3....12 4....14 4.1... 14 4.2... 14 4.3... 15 4.4... 16 5....17 5.1... 17 5.2... 17 5.3 Leica CM1850... 18 5.4... 20 5.5... 20 6....21 6.1 1... 21 6.2... 21 6.2.1... 21 6.2.2 ()... 22 6.2.3... 22 6.2.4 Peltier... 23 6.2.5... 23 6.2.6... 24 6.3... 24 6.4 2... 25 4 V 2.6 2012 10

7....26 7.1... 26 7.1.1... 26 7.1.2... 26 7.2... 27 7.2.1... 27 7.2.2... 27 7.3... 28 7.3.1... 28 7.3.2... 28 7.4 ( - C )... 29 7.5... 30 7.5.1... 30 7.5.2... 30 7.5.3... 31 7.6... 31 7.6.1... 31 7.6.2... 32 8....33 8.1... 33 8.2... 33 8.3... 34 9....39 9.1... 39 9.2 Leica Cryofect... 40 9.3... 41 9.3.1... 41 9.3.2... 42 9.3.3... 44 9.3.4... 44 9.3.5... 46 9.3.6... 46 10....48 10.1... 48 10.2... 50 10.2.1... 50 10.2.2... 50 11....51 12. ()...52 Leica C M1850 5

1. 1.1 (5) ( 5) 1.2 Leica CM1850 1.3 Leica CM1850 Leica CM1850 IVD () 1.4 1 6 V 2.6 2012 10

2. ( ) 2.1 / 2.1.1 2 1 (1) (2) 2 (1) 3 Leica C M1850 7

2. 2.1.2 CN CE CE-TC () 2.2 CE www.leicabiosystems.com 8 V 2.6 2012 10

2. 2.3 4 10 45 A 50 A 4 2.4 Leica C M1850 9

2. ( 31.12) 2.5 2.6 Leica Cryofect Leica Cryofect () 10 V 2.6 2012 10

2. 2.7 3 Leica C M1850 11

3. ()18 C 35 C 22 C 60% CM 1850 CM1850 CM1850-08 CM1850-01 CUL - - VDE (±10%) 120 V AC 220 V AC 230 V AC 240 V AC 60 Hz 60 Hz 50 Hz 50 Hz 1600 VA 1600 VA 1600 VA 1600 VA 5 45A eff. 45A eff. 45A eff. 45A eff. I I I I T12A T1 T12A T1 T10A T1 T10A T1 2 2 2 2 II II II II () 1600 J/s 1600 J/s 1600 J/s 1600 J/s IEC-1010UL 3101 50 Hz 60 Hz 0 C -35 C (+ 2 K / - 0 K) 0 C -35 C (+ 2 K / - 0 K) 1 / 24 1 / 24 690 W 690 W 3 3 300 g ±5 g R 404A* 300 g ±5 g R 404A* 0.6 l EMKARATE RL-22SICI * 0.6 l EMKARATE RL-22SICI * - 43 C (+ 3 K/- 2 K) - 43 C (+ 3 K/- 2 K) 10 10 Peltier -60 C (+5 K) -60 C (+5 K) 2 2* CECOMAF 45 C -25 C 12 V 2.6 2012 10

3. * 1 60 µm 25 mm 59 mm 55 x 55 mm 8 (x y z ) 200 µm/s 700 µm/s 50 Hz Osram Dulux S 11 W/21 LUMILUX 60 Hz Osram Dulux S 13 W/21 LUMILUX IEC-1010UL 3101 CECOMAF 45 C -25 C () () ( ) 600 mm 730 mm 730 mm 1140 mm 135 kg 5.2 5-55 C < 85% CM 1850 F1 T0.25 A Fa. SchurterFST 6.3x32 mm T0.25 A Fa. Littlefuse313 6.3x32 mm F2 T0.6 A Fa. SchurterFST 6.3x32 mm T0.6 A Fa. Littlefuse313 6.3x32 mm F3 T1.6 A Fa. SchurterFST 6.3x32 mm T1.6 A Fa. Littlefuse313 6.3x32 mm F4 T6.25 A Fa. SchurterFST 6.3x32 mm T6.25 A Fa. Littlefuse313 6.3x32 mm F5 T4 A Fa. SchurterFST 6.3x32 mm T4 Fa. Littlefuse313 6.3x32 mm Leica C M 1850 13

4. 4.1 10 4.2 1.5 () 35 C 60% 16 4 15 14 (14) (15) ( 30 ) (15) ( ) 14 V 2.6 2012 10

4. 4.3 CM1850 CM1850 ()... 14 0471 31148 1...14 0471 42558 1...14 0471 30792 1 ()...14 0471 30793 1...14 0470 43550 4 25 mm...14 0416 19275 4 30 mm...14 0370 08587 1...14 0471 30787 1...14 0471 30789 1...14 0471 30790 1...14 0398 13088 1...14 0436 43463 1...14 0183 28642 1...14 0183 40426 1 1.5...14 0222 10050 1 2.5...14 0222 04137 1 3.0...14 0222 04138 1 4.0...14 0222 04139 1 4.0...14 0222 32131 1 5...14 0222 04140 1 5...14 0194 04760 1 6...14 0022 04141 1 13...14 0330 33149 1 16...14 0330 18595 1 40750 ml...14 0336 06098 1 OCT 125 ml...14 0201 08926 1 Leica CM1850 G/E/F/S...14 0471 80001 CM 1850 Leica C M 1850 15

4. 4.4 1 2 3 4 5 (1) (2) (4) (3) 16 (5 mm) (4) (5) () 5 () 16 V 2.6 2012 10

5. 5.1 45 A 50 A 5.2 ( ) Leica C M1850 17

5. 5.3 Leica CM1850 17 13 15 4 16 21 20 8 13 13 12 19 10 6 18 14 9 11 18 V 2.6 2012 10

5. 3 5 6 2 1 7 1 CM1850 2 3 1 4 2 5 6 7 8 9 10 Peltier 11 12 13 14 () 15 16 17 18 () 19 CE () 20 CN () 21 CE-TC () Leica C M1850 19

5. 5.4 ( 1) ( 0) 7 5.5 4 00:00 10:00 () Peltier PE 6.2.1 6.2.3 20 V 2.6 2012 10

6. 6.1 1 8 5 6.2 6.2.1 9 1 ( ) Leica C M1850 21

6. 6.2.2 () 10 6.2.3 11 24 LED 15 1 5 22 V 2.6 2012 10

6.2.4 Peltier 12 6.2.5 Peltier Peltier 40 PE(=peltier ) Peltier 10(Peltier 10 ) 10 Peltier 4 4 (4.) Peltier PE 6. () () 13 Leica C M1850 23

6. 6.2.6 () () 14 6.3 5 LED 15 24 V 2.6 2012 10

6. 6.4 2 1 LED (1) LED (1) LED (2) 2 Leica C M1850 25

7. 7.1 7.1.1 1 () ( 7.4 ) ( 175) 10 2 5 Peltier (4) () 40 ( 18) 17 7.1.2 4 3 2 (2) (1) 30 (3) 26 V 2.6 2012 10

7.2 7.2.1 7. (1) (3) (2) (1) 7.2.2 18 (4) (5) (4) Leica C M 1850 27

7. 7.3 7.3.1 / 4 6 (CN CE-TC ) 1 2 (CE ) / (CN ) (CE CE-TC ) 7.3.2 (1) 1 60 µm 0 10 µm1 µm 10 20 µm2 µm 20 60 µm5 µm 20 µm 28 V 2.6 2012 10

7.4 ( - C ) 7. -10 C -15 C -15 C -25 C -25 C -35 C Leica C M1850 29

7. 7.5 ( 222) Peltier 12-5 C 7.5.1 7.5.2 24 1 ( 6.2.1 6.2.2 ) ( 6.2.6 ) 30 V 2.6 2012 10

7. 7.5.3 ( 6.2.5 ) 7.6 7.6.1 95 98% 1 ( 8) Leica C M1850 31

7. 7.6.2 ( 9 ) / 32 V 2.6 2012 10

8.1 20 8. EO: XX 20 21 22 23 (-35 C +55 C) 24 25 26 27 8.2 (1) 60 C 40 C --> 10 --> --> ( 9.3.1 ) 21 (1) Leica C M 1850 33

8. 8.3 ( ) / / ( 31 2) / / / / 34 V 2.6 2012 10

8. / / / / / / / / Leica CM1850 35

8. / / (c d ) / / 36 V 2.6 2012 10

8. / / - - 5.2 Leica CM1850 37

8. 5.2 10.3.1 38 V 2.6 2012 10

9. 9.1 ( 9.3.6 ) Leica Cryofect ( ) (1) 2 3 (1) (2) (3) 1 22 Leica C M 1850 39

9. 9.2 Leica Cryofect Leica Cryofect 1. -20 C 2. 3. 4. 5a. 5b. 6. 15 7. 8. 9. 40 V 2.6 2012 10

9.3 9.3.1 1 9. 3 23 4 2 ( 265) (1) 5 (T ) (2) (3) (4) 24 Leica C M 1850 41

9. 9.3.2 (5) ( 30) 9.3.6 42 V 2.6 2012 10

2 4 9. (4 mm) (3) (7) (1) (2) 3 1 8 8 25 7 5 (5) 26 Leica C M 1850 43

9. 9.3.3 8 40 C 50 C 8 8 8 (8) 27 9.3.4 (5) (5) (4) 44 V 2.6 2012 10

9. 2 (5) (6) (3) (1) (2) 4 5 6 1 3 29 28 Leica C M 1850 45

9. 9.3.5 3 5 29 F1 T 0.25 A F2 T 0.6 A F3 T 1.6 A F4 Peltier T 6.25 A F5 T 4 A 9.3.6 46 V 2.6 2012 10

9. 1 (2) (1) 3 2 30 4 5 3 6 (3) (4) (5) (6) 31 ( 32) 32 Leica C M 1850 47

10. 10.1...14 0419 26140 CN...14 0419 33993...14 0419 19426...14 0419 19427 50 mm 100 µm...14 0419 33981 50 mm...14 0419 33816 CE...14 0419 33990 CE...14 0419 33991 CE...14 0419 33992 B ()22...14 0502 29553 S ()22...14 0502 29551 70 mm 100 µm ()...14 0419 33980 70 mm 50 µm...14 0419 37258 70 mm 150 µm...14 0419 37260 70 mm...14 0419 33813 ( + 70 mm 100 µm)...14 0419 35693 CE-TC...14 0419 32073 ø 20 mm...14 0370 08636 ø 25 mm...14 0416 19275 ø 30 mm...14 0370 08587 ø 40 mm...14 0370 08637 ø 55 mm...14 0419 26491 50x80 mm...14 0419 26750 19x75x25 mm...14 0416 38207...14 0458 26771 () *...14 0471 30792 *...14 0471 30793 *...14 0443 26836...14 0398 18542 Miles-Adapter TissueTek...14 0436 26747 48 V 2.6 2012 10

10. CE...14 0800 37739 CE...14 0800 37740...14 0201 39115 18 mm...14 0201 39116 24 mm...14 0201 39117 30 mm...14 0201 39118 /...14 0201 39119 4 x 18 mm...14 0201 39120 4x 24 mm...14 0201 39121 3x 30 mm...14 0201 39122 28 mm...14 0201 39123 36 mm...14 0201 39124...14 0201 39125...14 0201 39126 8...14 0201 39127 CM1850...14 0201 39128...14 0201 39129...14 0712 40150...14 0712 40151...14 0712 40152...14 0712 40153...14 0712 40154...14 0712 40161 M...14 0340 29011 OCT 125 ml...14 0201 08926 407250 ml...14 0336 06100 Leica Cryofect4 x 250 ml...14 0387 36193 Leica C M1850 49

10. 10.2 10.2.1 33 10.2.2 (8) 8 5 6 7 (9) (5) (6) (7) 20 (10) 9 10 34 50 V 2.6 2012 10

11. Leica Biosystems Nussloch GmbH / Leica C M1850 51

12. () CJDBSECWD / KAT A B 1 / A B 2 * * 52 V 2.6 2012 10

12. () 3 / / RAN-/RGA SU (RGA) BU (RAN) / Leica Biosystems Nussloch GmbH Heidelberger Str. 17-19 69226 Nussloch, Germany ++49 (0) 6224 143 0 ++49 (0) 6224 143 268 www.leicabiosystems.com Leica C M1850 53

54 V 2.6 2012 10