1
2 Southern MAA SBD SBD Western SBD
3
4 48 2.5
5
6
7
8
9 α
10 µ α µ µ µ µ µ µµ µ µµ
11 β
12 µ µ µ µ µ µ
13 µ µ µ µ µ
14
µ 15
16 5 148 4 973
17 α β α β β αβ ααβ β ββ β α βα β
18 α λ µ µµµ µ µ µ λµ µ µ
µ µ 19 µ µ µ µ µ µ µ µ µµ µ µ µ µ
20
21 λ λ
22 µ λ µ µ µ µ
23 λ
24 β β
25 µ µ
26
27 µ µ
28 µ 28s rrna 18s rrna µ µ µ µ µ
29 µµ
30 µ µ µ µ
31 µ µ µ µµ µ µ µ µ µ µ µ µµ µ µ µ µµ
32 µ
33 µ µ µ µ
34
35 µ µ µ µ µ µ µ
36 µ µ
37 β β
38 µ µ µ µ
39 β
40 µ µ µ µ µ µ µ µ
41
42
43
44 µ
45
46 Southern Southern Southern Southern nitrocellulose filter membranenc Southern Southern Southern Southern α
47 µµµ
48 µ
49 µ µ µ µ µ α µ µ µ µ µ µ µ Ficoll
50 Southern µµ µµ µµ µµ µµ
51 Southern Southern
52 MAA MAA-DNA 1. (2004) : PCR, pp90-92,.
53 SBD : 18 ptrchis B(Bacillus circulans strain 251) (cyclodextrin glycosyltransferase, CGTase) (starch-binding domain, SBD) E.coli Top10 DNA T4 DNA (CGTase) (SBD) ptrchisb Top10 DNA ptrchisb/sbd DNA 1. (2004),. 2. Lawson, C.L., van Montfort, R., Strokopytow, B., Rozeboom, H.J., Kalk, K. H., de Vries, G.E., Penninga, D., Dijkhuizen, L. and Dijkstra, B. W. (1994) Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form. J. Mol. Biol. 236, 590-600. 3. Ji, Q.,l Vincken, J.-P., Suurs, L.C.J.M. and.visser, R.G.F. (2003) Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis. Plant Mol. Biol. 51, 789-801. 4. http://www.invitrogen.com.cn Invitrogen, ptrchis B
54 SBD 18 (PhastSystem)(ProBond Resin ) (RainBow),NAP-25 ()12.5%SDS-PAGEIPTG ( -β-d-) SBD SDS-PAGE SBD 1. (2004) :, pp93-97,. 2. Qin Ji, Jean-Paul Vincken, Luc C.J.M. Suurs, and Richard G.F.Visser (2003) Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis. Plant Mol. Biol. 51, 789-801. 3. http://www.invitrogen.com.cn Invitrogen, ptrchis B, ProBond Resin
55 Western 6 Western (PhastSystem), 12.5% SDS-PAGE (50mm 50mm 3mm), ()SBD Hybond ECL SBD () IgG () SBD SDS 1. (2004) :, pp98-101,. 4. Qin Ji, Jean-Paul Vincken, Luc C.J.M. Suurs, and Richard G.F.Visser (2003) Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis. Plant Mol. Biol. 51, 789-801.
56 6 Western (PhastSystem), 12.5% SDS-PAGE (50mm 50mm 3mm), () SBD SBD (Trypsin)Hybond ECL SBD () IgG () SBD, SDS 1. (2004) :, pp98-101,. 5. Qin Ji, Jean-Paul Vincken, Luc C.J.M. Suurs, and Richard G.F.Visser (2003) Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis. Plant Mol. Biol. 51, 789-801.
57 8 1. J.[] () () 22001 DNA. 3 http://wheat.pw.usda.gov/nsf/ 4. T Nakamura; P Vrinten; M Saito; M Konda / Rapid classification of partial waxy wheats using PCR-based markers//genome; Dec 2002; 45, 6; ProQuest Biology Journals, pg. 1150
58 18 DNA PCR DNA PCR, 1. J.[] () () 22001 DNA. 3 http://wheat.pw.usda.gov/nsf/ 4/ 2005
59 Å µ µ µ µ µ µ µ µ µ µ µ µ µ µ
60
61
62
63 ºµµ ºµµ ºµµ ºµµ ºµµ ºµµ µ
64
65
66
67
68
69 µ
70