33 7 207 7 Vol.33No.7 Jul.207 DOI:0.3652/j.issn.003-5788.207.07.00 Comparisononanti-microbialactivitiesofsixkindsofteasprocessed bythesameraw materials 2 PENG Ying-qi LONG Jun 2 YUAN Dong-yin LIN Ling ZHOU Yang GONG Zhi-hua 3 XIAO Wen-jun 3 (. 40282. 450003. 4028) (.Key Labof Tea Scienceof Ministryof EducationHunan Agricultural UniversityChangshaHunan4028 China2.Qi-liBridgeStreetOficeof Liu-yeLake TouristResortChangdeHunan45000China3.Hunan ColaborativeInnovation Centerfor Utilizationof BotanicalFunctionalIngredientsChangshaHunan4028China) : freshleavesgreenyelowdarkwhiteoolongandblacktea. 2 Theanti-microbialactivityagainstStaphylococcusaureus (S.au- 7 reus)escherichiacoli (B.subtilis)Escherichiacoli(E.coli) (Staphylococcusaureus) Aspergilusniger(A.niger)Saccharomycescerevisiae (S.cere- visiae)of7samplesweredetermindedbytheoxfordcup method. (Bacilussubtilis) (Escherichiacoli)3 TheMinimuminhibitoryconcentration(MIC)and Minimumbacteri- (Aspergilus niger ) cidalconcentration (MBC)of7samples weredetermined bythe (Saccharomycescerevisiae)2 96 methodoftwotimesdilutionusingthemicroplateofthe96hole.ex- 7 perimentalresultsshowedthat7sampleshadinhibitionefectofbac- (MIC) (MBC) :7 3 teriaandhadnoobviousefectofa.nigerand S.cerevisiae.Thean- 2 7 tibacterialcirclesizeof7samplesofs.aureusb.subtilisande. colishowedthatthetrendofantimicrobialactivitywas:fixationof > > > > > > freshleaves>green>yelow>dark>white>oolong>blacktea.the > > MICshowedthatthetrendofantimicrobialconcentrationwas:black > tea>oolongwhitetea>darkyelowgreenteafixationofleaves. TheMBCshowedthatthetrendofantimicrobialconcentrationwas: > blacktea>oolongwhitedarkyelowtea>greenteafixationof (P<0.05) leaves.theantimicrobialactivityofgreenteaandblackteawassig- : nificantlydiferentfromotherteas(p<0.05). Keywords:teaanti-microbialactivitiessixkindofteasminimum Abstract:Inordertostudytheanti-microbialefectofthesixkinds inhibitoryconcentrationminimumbactericidalconcentration ofteaunderthesameprocessingraw materialsthespringteafresh leavesofc.sinensiscv.bixiangzao wereprocessedintofixationof [] : ( :206NK202) : : (969 ) [2] [3] E-mail:xiaowenjun88@sina.com :207 05 02 [4] 47
207 7 [5] :LMQ.J3870C :SY-2000 [6] :0~200mm :LC-20A [7].2 [8].2. 2 3 [59-0] [] () : (2) : (250 ) (25 min) (20 ) (90 ) (60 ) [2]50 (3) : (250 ) (25min) (20 ) (60 ) [2]86-87 (4) : (250 ) (25 min) ( ) (33h) (25min) (60 ) [2]6 ( )2 ( ) (6) ( ): (20min) ( 5 ) (250 ). (20 ) (30 min) (80 ) (70 ).. : (7) : (5h) LB (40min) (30 3h) (85 ) :.2.2 : () :GB/T8304 203 (2) :GB/T8305 203 : Tedia : Tedia (epigalocatechingalate.2.3 GB/T8303 2002 EGCG) (galocatechingalate 3g 450 ml 00 45 min GCG) (epicatechingalateecg) 0min 500 ml (epecatechinec) (galocatechingc) (epigalocatechinegc) (cafeine ATCC6633(Bacilussubtilis) CICC305(Sac- charomycescerevisiae) CICC2039(Aspergilusni- ger):..2 0 7 CFU/mL :6CHP-94 :40.2.5 [8] :VD-650 0.mL :SPX-250BS- 3 0. ml 60 mg/ml :ZHWY-C20 37 24h 28 48h (5) : (48 h) (45 ) [2]204-207 (3) :GB/T8304 203 (4) : [3] [4] (5) : (55 0.9MPa)25mL 20mg/mL CA): >98%.2.4 ATCC25922(Escherichiacoli) ATCC25923 (Staphylococcusaureus) 37 8~24h 48 28 44~48h 4 :
33 7 : >20 mm 5~20 mm 0~5 mm 7~9mm <7mm 3.2.6 (MIC) [5] 96.2.7 (MBC) [6] 3.3 MicrosoftExcel SPSS22.0 2 82 00μL ~7 2 2. 20 mg/ml 00μL 2 00μL 3 3 5 3 00μL 2~2 0. ml [6] ~2 60305 3 0.70.0590mg/mL 37 (P<0.05) 24h 28 48h : > > : > > > 3 : > > >.2.6 96 3 00μL (P<0.05) 37 24h 28 48h 5 Table Anti-microbialcirclediameterofdiferentteasamples mm 6.05±0.22 b 7.04±0.55 b 6.22±0.78 b - - 5.35±0.8 c 6.80±0.2 b 6.5±0.42 b - - 4.7±0.7 d 5.40±0.72 c 4.42±0.27 c - - 3.29±0.8 e 4.86±0.24 c 4.35±0.37 e - - 3.00±0.32 e 3.30±0.33 d 4.08±.30 e - - 3.45±0.43 e 4.37±.30 c 4.78±0.9 c - - 8.00±0.00 f 9.80±.4 e 9.08±0.69 d - - CK( ) 23.00±0.42 a 34.77±0.67 a 28.52±0.2 a - - (P<0.05) - 7 (MBC) Table2 MICand MBCofdiferentteasampleson diferentmicroorganisms mg/ml [7] 0 mg/ml MIC MBC MIC MBC MIC MBC 00mg/mL.88.88 0.94 0.94 7.50 7.50 7 2.88.88 0.94 0.94 7.50 7.50.88 3.75 0.94 0.94 7.50 5.00 2.2 (MIC) (MBC).88 3.75.88.88 7.50 5.00 3.75 3.75 3.75 7.50 5.00 5.00 2 3.75 3.75 3.75 7.50 5.00 5.00 MICMBC 7.50 7.50 5.00 30.00 30.00 30.00 2 (MIC) 49
207 7 0.94.887.50 mg/ml 7.55 30 mg/ml 307.530 mg/ml.94 MIC : > >.26 5 MIC : > > EGCG GCG ECG > 3 MBC : > > (P<0.0) MBC : > > > 3 MIC MBC EGCG [9] [20-2] 7.8 [8] 2.3 [7] 4.63%3.57%3.37%5.49%3.78%3.94% 3 4 2.78 Table3 3 Mainbiochemicalcomponentsofdiferent teasamples % EGC DLC EC EGCG GCG ECG 6.56 28.35 3.92 40.60 6.23 26.23 3.85 4.99 6.88 26.09 4.40 39.92 6.38 24.92 3.84 39.45 5.65 22.95 4.52 38.7 5.54 23.5 4.70 38.4 3.55 0.20 4.82 29.00 5 4 Table4 Contentsofcatchinsandconstituentsin diferentteasamples % 0.452 0.45 0.25 9.882 4.840 2.347 8.87 0.492 0.298 0.207 9.248 5.537 2.393 8.76 0.458 0.262 0.325 8.94 5.08.909 6.66 0.425 0.36 0.76 6.33 4.29.388 2.63 0.59 0.080 0.4 6.54 2.665.72.28 0.365 0.25 0.84 7.2 3.682.869 3.427 0.0 0.05 0.97 0.265 0.32 0.340.058 Table5 Thecorrelationbetweenbiochemicalcomponentsandantibacterialcirclediameter EGC DLC EC EGCG GCG ECG 0.94 * * 0.978 * * -0.723 0.967 * * 0.79 * 0.793 * 0.74 0.990 * * 0.930 * * 0.967 * * 0.980 * * 0.886 * * 0.968 * * -0.72 0.979 * * 0.738 0.740 0.040 0.97 * * 0.90 * * 0.958 * * 0.956 * * 0.95 * * 0.955 * * -0.87 * 0.948 * * 0.83 * 0.88 * 0.25 0.956 * * 0.934 * * 0.924 * * 0.960 * * * 0.05 ( ) ** 0.0 ( ) 3 [2]. [J]. 206():8-365. [3]. (P<0.0) [J]. 2003(2):9-937. [4]. EGCG [J]. 20():0-2. [5]. [D]. : 203:37-39. []. [J]. 2009(3): 50 43-44. [6]. [J]. 2008 ():9-9462. ( 76)
207 7 4 ADAMS 8 Figure8 Displacementvelocityandaccelerationcurve ofmanipulatorliftingmovement 9 2009:3-4. Figure9 Contactforceandcontacttorquecurveof globoidalcam mechanism 0 [0]. ADAMS [J]. 2004(29):220-222. Figure0 Contactforceandcontacttorquecurveof liftinggloboidalcam mechanism []. [J]. 2003(2):32-33. [2]. [J]. 20632(6):77-82. [3]. [D]. : 2007:8-9. [4]. [D]. : [5]. VB6.0Pro/E [J]. 202(9):4-44. [6]. Pro/E [J]. 20640(7):97-00. [7]. [J]. 200220(5):30-33. [8]. Pro/E ADAMS[J]. 2008(4):35-36. [9]. [J]. 20733(2):77-80. []. [D]. : 204:43-60. ( 50) [7]. [6]. [J]. 200(2):63-67. [8]. [J]. 20(6):08-2. [9]. 996(4):86-88. [J]. 2002(2):35-3946. []. [M]. : 997:5. [2]. [M]. : 997. [J]. 2009(5):63-66. [J]. 205(5): 3-35. [7]. [J]. [8]. [0]. [J]. [J]. 203(7):737-74. 2009(5):26-267. [9]XIONGLi-guiCHEN Yi-junTONGJie-wenetal.Teapoly- phenolepigalocatechingaleteinhibitsescherichiacolibyin- creasing endogenous oxidative stress[j].food Chemistry [3]. 20727:96-204. [J]. 203(20):3-6. [20]. [4]. [M]. : [J]. 20632(9):54-60. 989:453-454. [2]. [5]. [J]. 20733(3):45-49206. 76