8 2 Vol. 8 No. 2 2017 2 Journal of Food Safety and Quality Feb., 2017 * 谢瑜杰, 呼秀智, 孙晓铮, 薛占永 (, 056021) 摘要 :,,, -,,, 关键词 : ; ; Research progress of application of colloidal gold assay in determination of sulfonamides residues in aquatic products XIE Yu-Jie, HU Xiu-Zhi, SUN Xiao-Zheng, XUE Zhan-Yong * (Hebei University of Engineer, Handan 056021, China) ABSTRACT: In recent years, due to the appearance and extensive use of antibiotics especially the wide usage of broad-spectrum and cheap sulfonamides in aquaculture for the prevention and treatment of bacterial diseases, it caused sulfonamides residues in aquatic products. This paper introduced sulfonamides and their harm, and briefly reviewed the application of colloidal gold immunochromatographic assay in the detection of sulfonamides in aquatic products. Compared with enzyme-linked immunosorbent assay (ELISA), high performance liquid chromatography (HPLC), liquid chromatography tandem mass spectrometry (LC-MS/MS) detection method and so on, colloidal gold immunochromatography technology is inexpensive, easy to use, fast and accurate, but its application is not so extensive, so how to improve the sensitivity of colloidal gold should be studied in the future, so that it can be able to detect a variety of drug residues in aquatic products. KEY WORDS: colloidal gold assay; sulfonamides residues; aquatic products 1 引言,,,, [1] [2], [1],, [3] * 通讯作者 :,,,. E-mail:407961849@qq.com *Corresponding author: XUE Zhan-Yong, Associate Professor, Master Tutor, Hebei University of Engineer, Handan 056021, China. E-mail: 407961849@qq.com
376 8,,, [4],, [5-7],,,, [8], 100 μg/kg, 20 μg/kg [5, 9-11] 2002 235, 0.1 mg/kg [7,11-13], [5] (enzyme linked immunosorbent assay, ELISA) [8] (high performance liquid chromatography, HPLC) [5] - (liquid chromatography- tandem mass spectrometry, LC-MS/MS) [10] [5] 2 磺胺类药物介绍及其危害 2.1 磺胺类药物的作用原理 [11], [12],, [13],,,,, [7],,,, [11] 2.2 磺胺类药物残留的危害 [14], [5] [15],,, [5], [5] ;, [7] ;, 3, [16,17], [7] ;,, [6] ;, Wang [9], 3 磺胺类药物残留检测的研究现状,,, [18] 3.1 常用检测方法 1993, 100 ng/kg, [7] 20, [19-21],, [13],,, [7] - [7,22],,, ;, [23] (enzymelinked immunosorbent assay, ELISA) [13] [24], ELISA, 3.2 胶体金免疫层析技术, [25] 20 80, Faulk Taylor [26-28] 3, [29] ELISA,,,, (10~15 min),,,,,, Geoghegan Danscher Faulk Taylor [28],, [30],,
2, : 377 [28] [5] [6], 2, (T) (C) [31], T C, T C,, ( 100 ng/kg) [6] ; T C,, ; T,, T T C, [6], [32],, T C, ;, [33] 噁, 50 ng/ml, 5~10 min [34] SD 10 ng/ml, 500 ng/ml,, 100% [35],, 20 ng/ml [36] [11], [28],, 8, [37] [5], [30], [38],,, [39-43] 4 展望, [44-47],, [48-50],, [30],, 参考文献 [1],,,. [J]., 2016, 7(5): 1976 1983. Gao LX, Huang ZK, Feng M, et al. Research progress of rapid detection in aquatic product safety testing [J]. J Food Saf Qual Test, 2016, 7(5): 1976 1983. [2],,. [J]., 2013, 12: 56 61. Zhang XY, Liu ZQ, Li Q. Application of colloidal gold immunochromatographic strip in aquaculture [J]. Biotechnol Bull, 2013, 12: 56 61. [3],,,. [J]., 2005, (5): 416 419. Li YD, Zhang SE, Wu ZG, et al. Rapid detection of chloramphenicol residue by colloidal gold immunochromatographic assay [J]. Chin J Food Hyg, 2005, (5): 416 419. [4]. [D]. :, 2013. Cao YY. Study on the detection of veterinary drug residues in animal food and the market investigation of veterinary drug residues in animal products sold in Jinan [D]. Jinan: Shandong University, 2013. [5]. [D]. :, 2010. Luo M. Application of colloidal gold immunochromatographic strip for detection of sulfonamides residues in aquatic products [D]. Wuhan: Huazhong Agricultural University, 2010. [6]. ELISA [D]. :, 2013. Du YL. A preliminary study on the rapid detection kit for sulfonamides multi residues ELISA and colloidal gold immunochromatographic strip [D]. Urumchi: Xinjiang Agricultural University, 2013. [7]. [D]. :, 2015. Chen JZ. Study on the detection of sulfonamides residues [D]. Shanghai: Shanghai Jiao Tong University, 2015. [8],,,. [J]., 2004, 14(1): 9 11. Guan R, Gu M, Wei WG, et al. Establishment of an ELISA for the detection of sulfadiazine residues in animal tissues [J]. Inspect Quarant Sci, 2004, 14(1): 9 11. [9] Wang L, Wang S, Zhang J, et al. Enzyme-linked immunosorbent assay and colloidal gold immunoassay for sulphamethazine residues in edible animal foods: investigation of the effects of the analytical conditions and the sample matrix on assay performance [J]. Anal Bioanal Chem, 2008, 390(6): 1619 1627. [10] Gao J, Cui Y, Tao Y, et al. Multi-class method for the quantification 92 veterinary anti microbial drugs in livestock excreta, wastewater, and surface water by liquid chromatography with tandem mass spectrometry [J]. J Sep Sci, 2016, 39(21): 4086 4095. [11]. [D]. :, 2011. Han J. Study on colloidal gold immunochromatographic assay of sulfonamides [D]. Tianjin: Tianjin University of Science and Technology, 2011. [12]. [D]. :, 2009.
378 8 Zhang JY. Development of a rapid test strip for the detection of sulfonamide veterinary immune colloidal gold [D]. Tianjin: Tianjin Univ Sci Technol, 2009. [13],. [J]., 2012, 03: 292 296. Zhao XZ, Li MY. Research progress on detection of sulfonamides residues in animal foods [J]. Chin J Food Hyg, 2012, 03: 292 296. [14] Li WL, Zhuang S, Lin H, et al. Matrix effects of apostichopus japonicus and its elimination methods in colloidal gold immunochromatographic assay for furazolidone metabolite [J]. Chin Fish Qual Stand, 2015. [15],,,. [J]., 2015, 26: 330 332, 335. Long J, Li PP, Long W, et al. Study on the determination of sulfonamides residues in animal derived food by chromatography [J]. Anhui Agric Sci, 2015, 26: 330 332, 335. [16],,. [M]. :, 2007. Wu YJ, Shao B, Shen JZ. Veterinary drug residue detection and monitoring technology [M]. Beijing: Chemical Industry Press, 2007. [17]. [J]., 2010, (2): 92 93. Wang RS. Harm and control of sulfa drugs residues in meat [J]. Vet Trib, 2010, (2): 92 93. [18]. [J]., 2011, (9): 29 30. Guo SJ. Research progress on detection methods of sulfonamides residues in aquatic products [J]. Heilongjiang Anim Husb Vet Med, 2011, (9): 29 30. [19] Cheng G, Wu X, Jin Z, et al. Simultaneous determination of three sulfonamide residues in modified milk by ultra performance liquid chromatography-tandem mass spectrometry [J]. Bull Kyushu Inst Technol Math Nat Sci, 2015, 31(45): 39 43. [20] Chen YP, Zhang SQ, Li LQ, et al. Imporvement of determination of sulfa drugs residues in aquatic products by UPLC method [J]. Hebei Fish, 2014, 2: 1. [21],,,. Determination of 7 kinds of sulfa drugs in a pork sample by hplc using a biphenyl core-shell stationary-phase column [J]. Bunseki Kagaku, 2016, 65(2): 87 92. [22] Guillén I, Guardiola L, Almela L, et al. Simultaneous determination of nine sulphonamides by LC-MS for routine control of raw honey samples [J]. Food Anal Methods, 2016, 1(2): 1 12. [23],,,. - [J]., 2007, 04: 508 510, 514. Li ZQ, Ni ML, Yu XJ, et al. Tandem mass spectrometry in aquatic products of sulfonamides and quinolones residues by liquid chromatography [J]. J Anal Test, 2007, 04: 508 510, 514. [24]. ELISA [D]. :, 2006. Liu BH. Development of rapid detection kit for sulfonamides residues ELISA [D]. Wuhan: Huazhong Agricultural University, 2006. [25] Mey JD. Colloidal gold probes in immunocytochemistryimmunocytochemistry 6 [M]. Wikipedia: Immunocytochemistry, 1983. [26]. [J]., 2011, (12): 22 24. Ren J. Research status and development of colloidal gold immunochromatographic assay [J]. Anim Husb Xinjiang, 2011, (12): 22 24. [27],. [J]., 2014, (10): 99 103. Fan SH, Wang YL. Progress in application of colloidal gold immunochromatography assay [J]. Adv Anim Med, 2014, (10): 99 103. [28],,,. [J]., 2004, 08: 33 35. Chen FM, Li J, Qu YJ, et al. Application and research progress of immune colloidal gold technique [J]. Chin J Vet Drug, 2004, 08: 33 35. [29],,,. [J]., 2016, 37(7): 1 4. Zhao YH, Liu HY, Ding ZF, et al. Development of colloidal gold immunochromatographic assay for the detection of residues in aquatic products [J]. Aquaculture, 2016, 37(7): 1 4. [30],,,. [J]. ( ), 2005, S1: 46 49. Wu J, Liu YG, Jia Q, et al. Application and research progress of immune colloidal gold technique in detection of livestock and poultry diseases [J]. J Southwest Univ Nation (Nat Sci Ed), 2005, S1: 46 49. [31],,. [J]., 2013, 08: 75 79. Zhang M, Li Q, Sheng HP. Preparation and application of the rapid detection reagent strip of colloidal gold for sulfonamides [J]. Chin Agric Sci Bull, 2013, 08: 75 79. [32],,,. [J]., 2011, 05: 603 606, 539. Han J, Liu EM, Wang S, et al. Determination of sulfonamides residues in foods by colloidal gold immunochromatographic assay [J]. Mod Food Sci Technol, 2011, 05: 603 606, 539. [33],,,. 噁 [J]., 2006, 40(4): 17 19. Zhang M, Wu GJ, Shen H, et al. Detection of sulfamethoxazole residues by immune colloidal gold [J]. China J Vet Med, 2006, 40(4): 17 19. [34],,,. [J]., 2008, 28(8): 76 76. Wang XL, Jin XE, Li K, et al. Colloidal gold strip semi-quantitative detection of residues in egg sulfadiazine [J]. Chin J Vet Sci, 2008, 28(8): 76 76. [35],,. [J]., 2009, 25(5): 577 580. Wu GH, He XL, Zhang SE. Rapid detection of sulfadiazine residue by colloidal gold immunochromatographyic assay [J]. Mod Food Sci Technol, 2009, 25(5): 577 580. [36]. [J]., 2014, (15): 7. Guo JJ. Application of colloidal gold immunochromatographic assay in the detection of drug residues in aquatic products [J]. Agric Beijing, 2014, (15): 7. [37],. [J]., 2001, 10(24): 2422 2423.
2, : 379 Zhan XQ, Qin XY. Application and development of colloidal gold labeling in immunoassay [J]. Mod J Integr Tradit Chin Western Med, 2001, 10(24): 2422 2423. [38] Du L, Zhu F, Li N. Colloidal gold immunochromatography assay for simultaneous detection of sulphonamides residue in pork [J]. J Chin Inst Food Sci Technol, 2014, 14(3): 151 160. [39],,,. [J]., 2013, (12): 179 182. Xue X, Rong X, Ye ZX, et al. Application of colloidal gold immunochromatographic assay in detection of veterinary drug residues in livestock and poultry products [J]. Adv Anim Med, 2013, (12): 179 182. [40],,,. [J]., 2014, 5(8): 2302 2307. Liu H, Li JC, Wu LD, et al. Application and development of rapid detection in the field of pesticide residues in aquatic products [J]. J Food Saf Qual Test, 2014, 5(8): 2302 2307. [41] Meng K, Sun W, Zhao P, et al. Development of colloidal gold-basedimmunochromatographic assay for rapid detection of Mycoplasma suis in porcine plasma [J]. Biosensors Bioelectr, 2014, 55: 396. [42]. [J]., 2014, (7): 46 50. Lu BK. Application and development prospect of colloidal gold immunochromatographic assay in the detection of veterinary drug residues [J]. Feed Expo, 2014, (7): 46 50. [43],,,. [J]., 2015, 40(2): 128 131. Zhou LY, Lv BX, Ren BR, et al. Application of colloidal gold immunochromatography assay in food safety detection [J]. China Condim, 2015, 40(2): 128 131. [44],,,. [J]., 2015, (5): 28 30. Jiang X, Li QZ, Qiu YH, et al. Research progress of colloidal gold immunochromatography assay [J]. Livestock Poultry Ind, 2015, (5): 28 30. [45] Li Q. Application of colloidal gold-based immunochromatographic lateral flow assay in aquaculture field [J]. Biotechnol Bull,2013, (12): 56 61. [46] Fan SH, Wang YL. Progress on colloidal gold immmunochromatographic assay [J]. Prog Vet Med, 2014, (10): 99 103.. [47] Wang YK, Shi YB, Zou Q, et al. Development of arapid and simultaneous immunochromatographic assay for the determination of zearalenone and fumonisin B 1 in corn,wheat and feedstuff samples [J]. Food Control, 2013, 31(1): 180 188. [48] Zhou C, Zhang X, Huang X, et al. Rapid detection of chloramphenicol residues in aquatic products using colloidal gold immunochromatographic assay [J]. Sensors, 2014, 14(11): 21872 21888. [49]. [D]. :, 2015. Cao S. Development of colloidal gold immunochromatography strip for sulfonamides [D]. Xiamen: Jimei University, 2015. [50]. [D]. :, 2015. Sun XK. The application of malachite green with gold immunochromatography and development in aquatic products [D]. Xiamen: Jimei University, 2015. 作者简介 ( 责任编辑 : 姚菲 ) 谢瑜杰, 硕士, 主要研究方向为食品安全与检测 E-mail: 1443908316@qq.com 薛占永, 副教授, 研究生导师, 主要研究方向为食品安全检查与动物健康养殖 E-mail: 407961849@qq.com