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Transcription:

理 CNIC9503 3 行 95 年 1 1 95 年 12 31 林 96 年 02 27

料 來 理 (1) (2) (3) 率 立 了 率 不 不 率 DPPH 力 TEAC OH 率 老 行 2 年 7% 度 GDP 率 兩 來 (1) 力 類 來 (2) 力量 行 略 (3) 理 行 - 1 -

來 老 行 老 年 領 類 利 類 老 流行 金 都 都 類 降 罹 率 不 了 率 - 2 -

留 率不 年 了 利 不 A. 1 DPPH 力 DPPH 藍 力 DPPH 來 力 若 DPPH DPPH 行連 DPPH 力 DPPH 力 不 度 利 度 (ELISA reader) OD 540 nm - 3 -

IC 50 力 DPPH 力 ( ) = [1- A540 nm, sample/a540nm, blank)] 100 2 TEAC 力 ABTS ABTS. + 離 藍綠 若 ABTS. + 離 力 降 力 trolox TEAC 力 力 力 不 度 利 度 OD 734 nm IC 50 力 力 ( ) = [1-(A734 nm, sample / A734 nm, blank)] 100-4 -

3 冷 力 I II III IV (a) (b) (c) (d) (e) (f) (g) 50µL I 量 1 ml II 量 1.6 ml III 量 100µL IV 量 量 數 量 冷 冷 數 10µL 冷 (h) 冷 量 冷 度 降 數 - 5 -

冷 ( A A intial - A sample ) 冷 (A intial ) 論 DPPH system TEAC system 力 DPPH system 度 0.088 µg/ml CF GR RR 率 85.6 % 83.9% 51.6% TEAC system 度 0.04 µg/ml GR CF TH 率 94.4 % 80.9% 76.0%( 1) DPPH system TEAC system GR CF 力 ME 力 GR CF sample sample - 6 -

離 行 力 Trolox EGCG Ascorbic acid 1. 力 度 率 DPPH system 0.088 µg/ml CF 85.6% GR 83.9% RR 51.6% TEAC system 0.04 µg/ml GR 94.4% CF 80.9% TH 76.0% 力 力 2 DPPH system HGR AGR EGCG 力 ACF Trolox 力 HME AME 力 TEAC system 類 來說 EGCG 力 HGR AGR EGCG 力 ACF 不論 DPPH system - 7 -

TEAC system 力 HCF 來 力 Trolox 2. DPPH system TEAC system IC 50 (ppm) DPPH system TEAC system HGR 1.44 1.66 AGR 1.45 1.75 HCF 6.81 4.07 ACF 4.42 3.36 HME 16.03 6.67 AME 16.55 5.01 Trolox 4.89 4.33 EGCG 1.58 1.77 Ascorbic acid 8.67 3.89 HGR AGR OH 力 EGCG Ascorbic acid 力 HCF Trolox OH 力 HME AME OH - 力 O 2 Trolox 數 參 H 2 O 2 力 HGR ACF HME 力 HCF AME 力 - 8 -

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