35 4 ( ' ) Vol.35No.4 204 2 JournalofYangzhouUniversity(AgriculturalandLifeScienceEdition) Dec.204 犐 狋 狅 犐 犓 狌 狉,,,, 2 (. /!"#$%, 22500; 2.& ', ( 430030)! ":)*+,-./0 234567897:;,- <( 犐 to)=>?@ab< <( 犐 Kur)CDE : 5~9=6~20 2 C 3, 犐 to 犐 Kur F G CHI JIKLM DE NO, P 犐 to 犐 KurQ R C S T U,V W X H I Y, < S C, Z[+50mV C <\ ] ^ 犐 toc S_`6JIabc C _`6LMab, CHI W=JI WCWU ], Y C YC GC ;,HI W=JI WCWU, F H YC,HI W=JI W ] 456789 犐 to 犐 KurC]=LMDE G #$%:45678; ; ;9;7;;./0 &' :R54.6 + ():A *+ :67 4652(204)04 004 05 犆 狅 狀 狋 狉 犻 犫 狌 狋 犻 狅 狀 狊 狅 犳 狋 犲 犿 狆 犲 狉 犪 狋 狌 狉 犲 狊 狋 狅 犐 狋 狅 犪 狀 犱 犐 犓 狌 狉 犻 狀 犺 狌 犿 犪 狀 犪 狋 狉 犻 犪 犾 犿 狔 狅 犮 狔 狋 犲 狊 犻 狊 狅 犾 犪 狋 犲 犱 犳 狉 狅 犿 狆 犪 狋 犻 犲 狀 狋 狊 狑 犻 狋 犺 犮 狅 狀 犵 犲 狊 狋 犻 狏 犲 犺 犲 犪 狉 狋 犳 犪 犻 犾 狌 狉 犲 犡 犝 犣 犺 犲 狀 犵 狓 犻 狀, 犣 犎 犝 犔 犻, 犣 犎 犈 犖 犌 犡 狌 犲, 犣 犎 犝 犔 犲 犻 2, 犑 犐 犖 犕 犪 狀 狑 犲 狀 (. 犑 犻 犪 狀 犵 狊 狌 犓 犲 狔 犔 犪 犫 狅 犳 犐 狀 狋 犲 犵 犜 狉 犪 犱 犆 犺 犻 狀 犲 狊 犲 犪 狀 犱 犠 犲 狊 狋 犕 犲 犱 犳 狅 狉 犘 狉 犲 狏 犪 狀 犱 犜 狉 犲 犪 狋 狅 犳 犛 犲 狀 犻 犾 犲 犇 犻 狊 / 犆 狅 犾 狅 犳 犕 犲 犱, 犢 犪 狀 犵 狕 犺 狅 狌 犝 狀 犻 狏,Yangzhou22500,China; 2. 犜 狅 狀 犵 犼 犻 犆 狅 犾 狅 犳 犕 犲 犱, 犎 狌 犪 狕 犺 狅 狀 犵 犝 狀 犻 狏 狅 犳 犛 犮 犻 犪 狀 犱 犜 犲 犮 犺,Wuhan430030,China) 犃 犅 犛 犜 犚 犃 犆 犜 :TostudythecharacteristicsoftransientoutwardK + current( 犐 to)andultra rapiddelayedrectifierk + current ( 犐 Kur)atdiferenttemperatureincongestiveheartfailure(CHF)patients, 犐 toand 犐 Kurwererecordedusingwhole celpatch clamptechnique.theresultsshowedthat 犐 toand 犐 Kurhaddistinctactivationorinactivationkineticpropertiesatdiferent temperature.compared withhighertemperature,theactivatingthresholdsof 犐 to and 犐 Kur atlowertemperature were lower,andthehalf maximalactivationpotentialsof 犐 toand 犐 Kur weresmaler,howevertheircurrent voltagerelationships werenotchanged.thecurrentdensitiesof 犐 to weresmalerwhendepolarizedto +50mV,andthatof 犐 Kur weresmaler eitherunderidenticalvoltage.butthevoltage dependentinactivationof 犐 to wasnotafectedbytemperature.activation timeconstantsorinactivationtimeconstantsof 犐 to weresmaleratlowertemperature,andtherelationshipsoftime voltagewerenotmodifiedobviously.however,theybecamegreaterwhentemperaturerose,anddecreasedwhenpoten tialincreased.wecoulddrawaconclusionfromthisexperimentthat 犐 toand 犐 Kurhaddiferentpropertiesat5-9 and 6-20. 犓 犈 犢 犠 犗 犚 犇 犛 :congestiveheartfailure;temperature;potassiumchannels;human;myocardium;patch clamptechniques <( 犐 to)=>?@ab< <( 犐 Kur) 97:;,- C < [ 4], <]=LMDE G,- Z ab=! C7 J [,5 8], C,-./:204 09 0 0 : ' (3077) : (964- ),, 9,, 75 = 犈 犿 犪 犻 犾 :xuzhengxin405@26.com
>4? @AB: X ] 犐 to 犐 Kur 5, 犐 to 犐 Kur [9] Beuckelmann [0]!", 犐 to #$%&', ( )*+35,,-./0../ Feng [] 2, 犐 Kur 3$4 5,06 789,37,:;"-./. 4 Li [2] <,36, 犐 to,= 3 $>?96 7@5 &ABCD, E./,F&GAHI JK LM NO,P @QRS TUV TU,W, <5~0 - X &Y LM W, Z[\\EX]^_` _abec_ [6 8],FA Yc_, Y, OG A W, ) 5~9 ( TU, )6~20 2 M, X ] 犐 to 犐 Kur, " ] B 7, TUV, 6, [3 4]H 2 (./ < EPC 9,. <00 MΩ,. <3.5~5.0 MΩ < X,././ >? V nifedipine (5μmol L - ) tetraethylammoniumchloride (TEA,0mmol L - ) BaCl 2 (mmol L - ), L./( 犐 Ca) /./( 犐 K) /./;./ (. (-80mV) -40mV 3$ 犐 Na,?./ 3!" V! " 7: T U V (mmol L - )< NaCl27 KCl20,MgCl 2.5 HEPES5 Glucose274,pH7.0;#$V(mmol L - )< NaCl40 KCl5.4 CaCl 2.8 MgCl 20.5 HEPES5.0 Glucose5.0,pH7.4;% #$ V < # $ V & CaCl 2 ' " ;. V (mmol L - )< Potassiumaspartate20 KCl20 MgCl 2.0 KH 2PO 4.0 Na 2ATP5.0 EGTA 5.0 HEPES5.0, ph7.2 4 #$%& SigmaPlot [,!= (94 狋 ),D* ) +< 犘 <0.05 2 ' & 2 ()*+,-./0- 犐 狋 狅 犐 犓 狌 狉 2. <-80mV -40 mv 20 ms,#,- +50 mv 300 ms &, :;" 犐 to 犐 Kur +5~96~20 2 M 7, O. /O,M Z[7 犐 to%&0 #$5,=./ 572 3456 %&,= 7 F89 :+; D 0 5~9, 犐 to8 9, 6~20, 犐 to 4 [,=:5~9 <(0.88±0.)ms;6~20 <(6.6±0.5)ms, 狀 = 2, 犘 <0.05],./ 4 [ +50 mv,:;"./ :5~9 <(6.5±0.9)pA/pF; 6~20 <(9.0±2.9)pA/pF, 狀 =2, 犘 <0.05], 72 3$4 [3$,=:5~ 9 <(2.3±0.8)ms;6~20 <(24±4)ms, 狀 =2, 犘 <0.05] 894, 犐 Kur 4 4S (5~9 ),./ 6< 4S[5~9 <(2.6 ± 0.6)pA/pF;6~20 <(3.5 ± 0.6)pA/pF, 狀 =2, 犘 <0.05], '7<8 (# $ 5,M3$^3$,./ 3*=4)
6 ZZ ([\0_] Z^)( _`[ZaZ ) >35b 2 2 *+,-./0- 犐 狋 狅 犐 犓 狌 狉 犐 犞 < +5~96~20 2 M Z[7,). < -80 mv,-40 mv 20 ms, #,- -40~ +50 mv C D 0 mv,? 300ms HE,#, :;" 犐 to 犐 Kur 犐 犞 O 2. 2/O,+2 M Z[7, 犐 to 犐 Kur 犐 犞 < FGHI,F 4M 0 5~ 9,89,6~20, 犐 to 犐 Kur+4A,#. 7J"2(5~9,, 犐 to 犐 Kur N+-30mV,#. 7 / D; +6~20,. N 3 ()0-456 789 犐 狋 狅 犐 犓 狌 狉 :;3 G -20 ^ 0 mv F J / " 2 犐 to^ 犐 Kur), 犐 犞 犉 犻 犵. 犚 犲 犮 狅 狉 犱 犻 狀 犵 狊 狅 犳 犱 犲 狆 狅 犾 犪 狉 犻 狕 犪 狋 犻 狅 狀 犻 狀 犱 狌 犮 犲 犱 KLM. N N (5~9, KL8 狋 狉 犪 狀 狊 犻 犲 狀 狋 狅 狌 狋 狑 犪 狉 犱 狆 狅 狋 犪 狊 犻 狌 犿 犮 狌 狉 犲 狀 狋 ( 犐 狋 狅 ) 犪 狀 犱 狌 犾 狋 狉 犪 狉 犪 狆 犻 犱 XO*).W G,, 犐 to 犐 Kur # $ 犱 犲 犾 犪 狔 犲 犱 狉 犲 犮 狋 犻 犳 犲 狉 狆 狅 狋 犪 狊 犻 狌 犿 犮 狌 狉 犲 狀 狋 ( 犐 犓 狌 狉 ) 犳 狉 狅 犿 犺 狌 犿 犪 狀 (:;" 犐 to 犐 Kur. )N,,M;. N,. / F G 4 5,. /. 犪 狋 狉 犻 犪 犾 犿 狔 狅 犮 狔 狋 犲 狊 犪 狋 犱 犻 犳 犲 狉 犲 狀 狋 犲 犿 狆 犲 狉 犪 狋 狌 狉 犲 A.5~9 ;B.6~20 N? 32 ) 犐 狋 狅 犐 犓 狌 狉 犐 犞 => 犉 犻 犵.2 犆 狌 狉 狉 犲 狀 狋 狏 狅 犾 狋 犪 犵 犲 狉 犲 犾 犪 狋 犻 狅 狀 狊 犺 犻 狆 狊 犳 狅 狉 犐 狋 狅 狅 狉 犐 犓 狌 狉 犪 狀 犱 狋 犲 狊 狋 狆 狅 狋 犲 狀 狋 犻 犪 犾 犲 狓 犪 犿 犻 狀 犲 犱 犪 狋 犱 犻 犳 犲 狉 犲 狀 狋 狋 犲 犿 狆 犲 狉 犪 狋 狌 狉 犲 狊 A.,#H :;" 犐 to (F <5~9 ;7 <6~20 );B. 犐 to 犐 Kur I V GJ ( 犐 to< P Q; 犐 Kur<RS P Q; 2.5~9 ;3 4.6~20 ; 狀 =2) 2 3 *+,-./0- 犐 狋 狅 犐 犓 狌 狉 6? <,#H 2.2T * 犐 to 犐 Kur./ U <.;( 犌 ), V. 7.; X8. W,X!34#$ ( 3) BoltzmannH? 犌 =/{+exp[-( 犞 - 犞 /2)/ 犽 ]} XV,Y" #$. ( 犞 /2)KL( 犽 )( 犞 <. ). 3/O,2 M Z[7 犐 to^ 犐 Kur#$ 8,F M 894,5~9, 犞 /24 [ 犐 to:5~9 <(4.4±0.8)mV,6~20 <(29±4)mV, 狀 =2, 犘 <0.05; 犐 Kur:5~9 <(7±3)mV,6~20 <(35±5)mV, 狀 =2, 犘 <0.05], 犽 4 [ 犐 to:5~ 9 <(7.8±0.8)mV,6~20 < (±3)mV, 狀 =2, 犘 <0.05; 犐 Kur:5~9 < (7.±.)mV,6~20 <(0.±.9)mV, 狀 =2, 犘 <0.05] 2 4 *+,-./0- 犐 狋 狅 @? <. <-80mV,-80~0mV CD<0mV,?<2000ms Z[,- +60mV,?<300ms, :;" 犐 to X X8 Z[. W,X 343$ ( 4) BoltzmannH?
>4? @AB: X ] 犐 to 犐 Kur 7 犐 =/{+exp[( 犞 - 犞 /2)/ 犽 ]} XV,Y" 3$. ( 犞 /2) KL( 犽 ) + 2 M (5~9 6~ 20 )7. 4/O,+M Z[7,2Z 4 8, " 犞 /2^ 犽 8 [5~9 < 犞 /2=-(46.2±.9)mV, 犽 =(2.9±0.3)mV;6~ 20 < 犞 /2=-(44±5)mV, 犽 =(.7±0.6)mV], 89%D* (( 犘 >0.05, 狀 =2) 33 犐 狋 狅 犐 犓 狌 狉 A ) BCD,6? 2 5 *+,-./0- 犐 狋 狅 E CD 犉 犻 犵.3 犞 狅 犾 狋 犪 犵 犲 犱 犲 狆 犲 狀 犱 犲 狀 狋 犪 犮 狋 犻 狏 犪 狋 犻 狅 狀 犮 狌 狉 狏 犲 狅 犳 犐 狋 狅 犪 狀 犱,6? < 犐 犓 狌 狉 犪 狋 犱 犻 犳 犲 狉 犲 狀 狋 犲 犿 狆 犲 狉 犪 狋 狌 狉 犲 +2 M (5~96~20 )Z[7,) 犐 to. ^H P Q; 犐 Kur.ARS ^crs P Q; 2.5~9 ;3 4.6~20. <-80mV,-40 mv 20 ms, #,- -40~ +60 mv C D 0 mv, = 300ms L2 Hz H E, #, ( 犐 to! H? 犐 = 犐 max[-exp(- 狋 /τ)]+ 犮 X *. /( 犐 ), =( 狋 ),Y ". 7 # $, = P (τ) V. 7 τ X8. W, /X #$,=P. GJ ( 5). 5 / O,+ 4 (6~20 )Z [ 7,+ -20~ +0 mv.,m ;, #. N, τ,89 2 < 8 G(8 G J < -0.9733), N., τ M D F + 4 S (5~9 )7,τ 8X3*,M M, #. _ D 894, 4S, τ 4 2 6 *+,-./0- 犐 狋 狅 E CD,@? < F,# H : ; " 犐 to, V. 7 M, =./ H? 犐 = 犐 maxexp(- 狋 /τ),y". 7 犐 to 3$,=P (τ) V. 7 τ X 8. W, / X 3$ LP. GJ ( 6) 34 犐 狋 狅 A ) BCD,@? 犉 犻 犵.4 犞 狅 犾 狋 犪 犵 犲 犱 犲 狆 犲 狀 犱 犲 狀 狋 犻 狀 犪 犮 狋 犻 狏 犪 狋 犻 狅 狀 狅 犳 犐 狋 狅 犪 狋 犱 犻 犳 犲 狉 犲 狀 狋 狋 犲 犿 狆 犲 狉 犪 狋 狌 狉 犲 狊.5~9 ;2.6~20 ; 狀 =2 35 犐 狋 狅 E CD,6? 犉 犻 犵.5 犜 犻 犿 犲 犱 犲 狆 犲 狀 犱 犲 狀 狋 犪 犮 狋 犻 狏 犪 狋 犻 狅 狀 狅 犳 犐 狋 狅.5~9 ;2.6~20 ; 狀 =2. 6/O,+4S (5~9 )=47,V. 7 3$,=P τ % D F G (6~20 ),M;,#. N,τ,89 2< 8G(8GJ <-0.9750) 894, 4S, τ 4 3, _ 3P.. V./, a, 3P _, *&./ < < +4S 7,,A._a &/E. :,+ 6Z[7,
8 ZZ ([\0_] Z^)( _`[ZaZ ) >35b G (5~9 FG 6~20 ),,8. 7 犐 to 犐 Kur./ <M N, 犐 犞 4 _,#$ N,./. N? #$. <N, 犐 to,= #$3$>? M (6~20,,M;,#. N,# $, = P 3 $, = P,F + 5~ 9,, F MM,#. _ D ).W,+M Z[7, 犐 to 犐 Kur+./ 犐 犞 # $, = # $ ^ 3 36 犐 狋 狅 E CD,@? $>? H I < : + ; D ( X 犐 to 犉 犻 犵.6 犜 犻 犿 犲 犱 犲 狆 犲 狀 犱 犲 狀 狋 犻 狀 犪 犮 狋 犻 狏 犪 狋 犻 狅 狀 狅 犳 犐 狋 狅 犪 狋 犪 狀 犱 犇 犪 狋 犪 犐 Kur %&.5~9 ;2.6~20 ; 狀 =2., =4. F8G [9,2,5] W9 <, /X c_ W, LM /E& 74 :. E B, XE c_ _, 0E &G >?, W, / E _, X,# 2, F:+;@N &, A & >? 46 >? & >?/ =4, 6 >?, W, E ATP M; _8, =M 3 M, F &? /E:+ (,.W X + M 7 _ 4,, E _,A + F ; > E,./ Z>? [2] &,M ; G (23 36 ), 犐 to # $ N 5 F +, 5~9 6~20,:;" 8. 7 #$,=P 94,+4S 7./ #$ 4 5,0 8 /E :. 5~9 P = 4, W,+ S 7, 犐 to _,X.,# M _ 2 4S, _, 犐 to AP Z7 3 4S, 犐 to _,: = _8 4.,S =47 F / _,.W " 5 5~9 6~20, 02~23 37,M,A, B &,. /X"A : 06~20 89,5~9, %& NB 7 W9 A, + W M Z[7 7 8 F:!"#$%&' $, ()*+! GHIJ: [] FirekL,Giles W R.Outwardcurrentsunderlyingrepolarizationinhumanatrialmyocytes [J].CardiovascRes, 995,30():3 38. [2] Xu W H,LiW,YueH W,etal.CharacteristicsoftransientoutwardK + currentinhumanatrialcardiomyocytes [J].ActaPharmacolSin,998,9(5):48 485. [3] WangZG,FerminiB,NatelS.Sustaineddepolarization inducedoutwardcurrentinhumanatrialmyocytes [J]. CircRes,993,73(6):060 076. (,-.36/)