南方水产科学 52 篮子鱼的研究报道 [ 2-6] 第9卷, 笔者运用常规解剖学组织学和组织化学技术, 对黄斑篮子鱼消化道的形态组织学组织化学结构进行了观察研究, 旨在进一步了解该鱼的消化生理学特性, 充实黄斑篮子鱼的养殖生物学资料 1 材料与方

Size: px

Start display at page:

Download "南方水产科学 52 篮子鱼的研究报道 [ 2-6] 第9卷, 笔者运用常规解剖学组织学和组织化学技术, 对黄斑篮子鱼消化道的形态组织学组织化学结构进行了观察研究, 旨在进一步了解该鱼的消化生理学特性, 充实黄斑篮子鱼的养殖生物学资料 1 材料与方"

宣室韦
5 years ago
Views:

1 South China Fisheries Science Vol. 9, No. 5 Oct., 2013 doi: / j. issn ,,,, (,, ) : HE AB-PAS ( Siganus oramin),,,,,,, V, ,,,, AB-PAS 4 :,,,,, > >, : ; ; ; ; : S : A : ( 2013) Histological and histochemical study of digestive tract of Siganus oramin OU Youjun, LI Jia er, GOU Xiaowei, MA Zhiming, CHEN Yibiao ( Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; Key Lab. of Fishery Ecology Environment, Guangdong Province; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou , China) Abstract: We studied the histological structure and histochemistry in the digestive tract of Siganus oramin by using HE and AB-PAS in this paper. Results show that S. oramin has comparatively small orpharyngeal cavity, with slight teeth on maxillary, short and closely arranged teeth on mandible, no tooth on vomer, palatine bone and tongue, soft and slight upper pharyngeal teeth, slight oesophagus, and the V-shaped stomach. 5 6 pyloric caeca are found on the intestine. Ratio of intestine length to body length is The gastric gland is developed and pipe shaped, opening to the gastric pit. Height of mucosa membrane pleats lowers on pyloric stomach and villous processus-like structure occurs on the pleats. There are abundance mucosa pleats on the surface of foregut, and plenty of vacuolelike goblet cell. According to AB-PAS, 4 types of the mucous cells in the digestive tracts of S. oramin are observed: Typeis red, Typeis blue, Type is purple reddish, and Typeis blue purple. Most of mucous cells in oesophagus are of Type and Type and those in stomach, intestine and pyloric caeca are of Typeand Type. The density of mucous cells in hindgut are found to be Type> Type > Type. The ratio of Typeto mucous cells is the biggest in midgut and hindgut. Key words: Siganus oramin; digestive tract; anatomy; histology; histochemistry [ 1 ] ( Siganus oramin),,,,,, : ; : : ( A200901D01 ) : ( ),, ouyoujun@ 126. com

南方水产科学 52 篮子鱼的研究报道 [ 2-6] 第9卷, 笔者运用常规解剖学组织学和组织化学技术, 对黄斑篮子鱼消化道的形态组织学组织化学结构进行了观察研究, 旨在进一步了解该鱼的消化生理学特性, 充实黄斑篮子鱼的养殖生物学资料 1 材料与方法供试动物黄斑篮子鱼共 3 尾, 于 2006 年 7 月 8 日采集自广州市黄沙水

2 南方水产科学 52 篮子鱼的研究报道 [ 2-6] 第9卷, 笔者运用常规解剖学组织学和组织化学技术, 对黄斑篮子鱼消化道的形态组织学组织化学结构进行了观察研究, 旨在进一步了解该鱼的消化生理学特性, 充实黄斑篮子鱼的养殖生物学资料 1 材料与方法供试动物黄斑篮子鱼共 3 尾, 于 2006 年 7 月 8 日采集自广州市黄沙水产市场, 体长范围 cm, 体质量 g 将试验鱼置于实验室的水槽中暂养 24 h, 待其排空消化道中的食物后解剖, 取出消化道, 测量肠长比 ( 肠长与体长之图1 黄斑篮子鱼口咽腔的水平剖面比), 从食道胃( 贲门部盲囊部幽门部) 幽 A. 背面 ; B. 腹面; 1. 上颌齿 ; 2. 下颌齿 ; 3. 舌 ; 门盲囊和肠道等部位切取组织块, 放入 10% 中性 4. 鳃耙 ; 5. 鳃弓 ; 6. 上咽骨齿甲醛溶液中固定; 经乙醇脱水二甲苯透明以及石 Fig. 1 Cross-section of oral-pharyngeal cavity in S. oramin A. dorsal side; B. ventral side; 1. incisor; 2. molar; 蜡包埋系列处理后用 Leica 切片机连续切片, 切片 3. tongue; 4. gill raker; 5. branchial arch; 厚度为 5 μm, 常规 HE 染色; 采用 AB-PAS 反应显 6. upper pharyngeal tooth. 示粘液细胞在 Leica 显微镜下进行观察和拍照, 使用目微尺对随机取的 15 张切片样本进行测量; 细胞密度以每一视野中 100 μm 100 μm 范围内细胞的总数 100 换算所测数据采用 SPSS 软件进行统计分析 2 结果消化系统外部形态 2. 1 图2 黄斑篮子鱼口小, 口咽腔不宽阔上颌具 1 行细长尖锐齿, 下颌齿短, 排列比上颌齿紧 7. 后肠; 8. 肝 ; 9. 幽门盲囊 ; 10. 胆囊密舌细长, 前段有极小部分游离犁骨腭骨和舌上无齿上咽骨齿细长, 柔软鳃耙齿分黄斑篮子鱼的消化道 1. 食道 ; 2. 贲门; 3. 胃盲囊 ; 4. 幽门 ; 5. 前肠 ; 6. 中肠 ; Fig. 2 Digestive tract in S. oramin 1. oesophagus; 2. cardia; 3. stomach caeca; 4. pyloric 叉, 非骨质, 较柔软 ( 图 1) 食道细长, 内壁有 stomach; 5. foregut; 6. midgut; 7. hindgut; 8. liver; 明显的纵行褶皱胃部稍膨大, 呈 V 字型, 幽 9. pyloric caeca; 10. gallbladder. 门盲囊 5 6 条, 肠道盘绕于体腔内, 肠道周围及肠道团外侧都覆盖有大量脂肪肠长比为则, 细胞核大, 位于细胞基部, 其间可见杯状细肝脏发达, 棕黄色, 覆盖于肠道团上方胞固有膜与粘膜下层之间的分界不明显, 很难辨胆囊椭圆形, 深蓝色( 图 2) 别, 故将固有膜和粘膜下层合并在一起测量, 厚度消化道组织学特征为 60 μm 粘膜下层由疏松结缔组织构成, 其间有 2. 2 黄斑篮子鱼的食道位于其口咽之丰富的血管和神经细胞; 在粘膜下层还发现有许多后, 贲门之前, 组织结构由内向外分为粘膜层粘纵行的平滑肌纤维束肌层为横纹肌, 厚度为膜下层肌层浆膜 4 层食道内表面分布有许多 μm, 内层环肌的分布断续, 外层纵肌的厚较为细长的粘膜褶, 约 10 个, 无分支粘膜上皮度不均浆膜层均比较薄, 厚度为 7. 1 μm, 由间为多层细胞结构, 厚度为 μm, 表层为单层皮细胞和结缔组织构成, 容易脱落( 图 3 - a) 柱状上皮, 在单层柱状上皮下存在正染色胞质呈蓝色的嗜酸性杯状细胞; 其下多层细胞排列不规织同样由 4 层组成贲门部的粘膜上皮为排列紧密食道胃胃呈 V 形, 胃盲囊不发达, 胃组

3 5 : 53 3 ( HE ) a. ; b. ; c. ; d. ; e. ; f. ; g. ; h. ; BV. ; CL. ; CM. ; GG. ; GP. ; GC. ; LM. ; ME. ; MFB. ; NC. ; S. ; SM. Fig. 3 Cross section of digestive tract of S. oramin ( HE staining) a. oesophagus; b. cardiac gastric pit and gastric gland; c. gastric caecum; d. mucous fold at pylorus; e. foregut; f. midgut; g. hindgut; h. pyloric caeca; BV. blood vessel; CL. central lacteal; CM. circular muscle; GG. gastric gland; GP. gastric pit; GC. goblet cell; LM. longitudinal muscle; ME. mucosal epithelium; MFB. muscular fibre beam; NC. nerve cell; S. serosa; SM. submucosa,,, ;, ;, ;,,,, ; ; mm - 2,,,, ;,, 1 2,, ( 3 - e),, ( 3 - b),, ; ; ( 3 - c ),,,, mm - 2 ;,, ( 3 - f) ;, ; ( 3 - d), ,, , mm - 2,, 35. 4

54 9,,,,,,, 245. 0 mm - 2, ( 3 - g) ( 3 - h) 2. 2. 6,,, 1 1 ( X SD) Tab. 1 Histological features of digestive tracts of S. oramin section foregut midgut hindgut pyloridc caeca / mucosa number 36. 6 4.

4 54 9,,,,,,, mm - 2, ( 3 - g) ( 3 - h) ,,, 1 1 ( X SD) Tab. 1 Histological features of digestive tracts of S. oramin section foregut midgut hindgut pyloridc caeca / mucosa number / m mucosa height / m mucosa width / mm - 2 goblet cell density / mm - 2 mucusa cell density / m mucosal epithelium thickness / m lamina propria + submucosa thickness / m muscular coat thickness ,,,,,,, +,, ( 4 - b, c, d) , 2. 3 [ 7] AB- PAS,,, 4 : AB-PAS 96. 2%, (, PAS ; 4 - f), AB ; , PAS,, AB ; 91. 5% ( 4 - g), AB PAS, % ( 4 - h) ( ) ( ),,,,, ( 4 - a) 2, 84. 1% ( 4 - e) , > >, ( 2)

5 5 : 55 4 ( AB-PAS ) a. ; b. ; c. ; d. ; e. ; f. ; g. ; h. ;. ; Fig. 4. ;. ;. Cross section of digestive tract of S. oramin ( AB-PAS staining) a. oesophagus; b. cardiac stomach; c. undic stomach; d. pyloric stomach; e. foregut; f. midgut; g. hindgut; h. pyloric caeca;. type mucous cell;. typemucous cell;. type mucous cell;. typemucous cell 2 ( X + SD) Tab. 2 Density of mucous cells in digestive tract of S. oramin mm - 2 section type type mucous cell type type foregut midgut hindgut pyloridc caeca total ,,, HE, [ 9],,, [ 3 ],,, V, 2. 73, ( Sparus aurata) ( Chrysophrys, major), 2,,, [ 1 0], ; ( Cyprinus carpio) [ 8],,,

6 56 9,, [ 11] ( Cromileptes altivelis) [ 12 ] ( Trachinotus ovatus) [ 13 ], +,,,,, [ 10] [ 1 7], [ 14 ],, KITZAN SWEEN [ 18 ], PAS [ 10] ( Protopterus annectens), HID AB-PAS 3 :,, SIBBING URIBE,,,, [ 7 ],, AB-PAS 4 ( MURRAY [ 15] ( Hippoglossus hippoglossus) ( Pleuronoctes feeruginea) ( Pleu-, rooctes americanus),,,, ;,,,, 3. 3,,, [ 1 2],,, SINHA [ 20 ], [ 16 ], HE ( Labeo calbasu) AB-PAS 4 AB-PAS,,,,,, AB-PAS AB, PAS, 2,, 3 SIBBING URIBE [ 1 9] ),,

5 : 57 : [ 1],,. [ M]. : [ 13],,., 1995: 898-900. [ 2]. Siganus oramin ( Bloch & Schneider) [ J]. : [ 14],,,., 1998( ) : 4-9. [ J]., 1999, 14( 4 ) : 1-16.

7 5 : 57 : [ 1],,. [ M]. : [ 13],,., 1995: [ 2]. Siganus oramin ( Bloch & Schneider) [ J]. : [ 14],,,., 1998( ) : 4-9. [ J]., 1999, 14( 4 ) : tological and histochemical study of the post-gastric alimentary ca- [ 4],,. nal from three species of pleuronectid, the Atlantic halibut, the [ J]., 2007, 42( 4 ) : [ 5],,,. 1996, 48 ( 2) : [ J]., ( 3 ) : [ 6],,,. [ J]., 2001, 47( ) : [ J]., 2010, 34 ( 2) : [ 7],,. 1999, 23 ( 4) : [ J]., 1997( ) : [ 8] KAPPOR B G, SMITH H, VERIGINA I A. The alimentary canal and digestion in teleosts[ J]. Adv Mar Biol, 1976, 13: [ J]., 2006, 30( 5) : [ 10],,. [ J]. Netherlands J Zool, 1985, 35( 3 ) : [ J]., 1997, 21 ( 2) : [ 11],,,. [ M]. :, 1990 : [ J]., ( 3 ) : [ 12],,. 1977, 23 ( 3) : [ J]., 2012, 27( 1) : [ J]., 2007, 31( 3) : [ 3],,,. [ 15] MURRAY H M, WRIGHT G M, GOFF G P. A comparative his- yellowtail flounder and the winter flounder [ J]. J Fish Biol, [ 16],,. [ 17],. [ J]., [ 18] KITZAN S M, SWEEN P R. A light and electron microscope study of the structure of Protopterus annectens epidermis.. mucus production[ J]. Can J Zool, 1968, 46 ( 4) : [ 9],,,. [ 19] SIBBING F A, URIBE R. Regional specializations in the ero-pharyngeal wall and food processing in the carp ( Cyprinus carpio L. ) [ 20] SINHA G M. Functional histology of the different regions of the oesophagus of a freshwater major carp Labeo calbasu ( Hamilton) during the different life history stages [ J]. Zoologische Beitrage,

Microsoft Word - 13-区又君.doc

Microsoft Word - 13-区又君.doc 热带海洋学报 JOURNAL OF TROPICAL OCEANOGRAPHY 海洋生物学 doi:10.3969/j.issn.1009-5470.2012.06.013 2012 年第 31 卷第 6 期 : 83 89 http://www.jto.ac.cn 波纹唇鱼消化道的形态学和组织学区又君 1, 廖光勇 1,2, 李加儿