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1 碧云天网站 微信公众号 碧云天生物技术 /Beyotime Biotechnology 订货热线 : 或 订货 技术咨询 网址 : BCA 蛋白浓度测定试剂盒 产品编号产品名称包装 P0011 BCA 蛋白浓度测定试剂盒 5000 次 产品简介 : BCA 蛋白浓度测定试剂盒 (BCA Protein Assay Kit) 是根据目前世界上最常用的两种蛋白浓度检测方法之一 BCA 法研制而成, 实现了蛋白浓度测定的简单 高稳定性 高灵敏度和高兼容性 灵敏度高, 检测浓度下限达到 25µg/ml, 最小检测蛋白量达到 0.5µg, 待测样品体积为 1-20µl 在 µg/ml 浓度范围内有较好的线性关系 BCA 法测定蛋白浓度不受绝大部分样品中的化学物质的影响, 可以兼容样品中高达 5% 的 SDS,5% 的 Triton X-100,5% 的 Tween 但本试剂盒受螯合剂和略高浓度的还原剂的影响, 需确保 EDTA 低于 10mM, 无 EGTA, 二硫苏糖醇 (DTT) 低于 1mM,β- 巯基乙醇 (β-mercaptoethanol) 低于 0.01% 不适用 BCA 法时建议试用碧云天生产的 Bradford 蛋白浓度测定试剂盒 (P0006) BCA 蛋白浓度测定试剂盒对样品中各种物质的详细的兼容性, 请参见碧云天如下网页 : Chart For BCA Kit.pdf 每个试剂盒可以检测 5000 个样品 包装清单 : 产品编号 产品名称 包装 P BCA 试剂 A 500ml 2 P BCA 试剂 B 30ml P 蛋白标准 (BSA) 30mg 2 P 蛋白标准配制液 5ml 说明书 1 份 保存条件 : 室温保存 蛋白标准配制成溶液后 -20ºC 冻存 注意事项 : 需酶标仪一台, 测定波长为 nm 之间,562nm 最佳 需 96 孔板 如果没有酶标仪, 也可以使用普通的分光光度计测定, 但测定时, 需根据比色皿的最小检测体积, 适当加大 BCA 工作液的用量使不小于最小检测体积, 样品和标准品的用量可相应按比例放大也可不变 使用分光光度计测定蛋白浓度时, 每个试剂盒可以测定的样品数量可能会显著减少 如发现样品稀释液或裂解液本身就有较高背景, 请试用碧云天生产的 Bradford 蛋白浓度测定试剂盒 (P0006) 为了加快 BCA 法测定蛋白浓度的速度可以适当用微波炉加热, 但是切勿过热 EDTA 浓度必须小于 10mM, 不兼容 EGTA 不适用 BCA 法时, 请试用碧云天生产的 Bradford 蛋白浓度测定试剂盒 (P0006) 本产品仅限于专业人员的科学研究用, 不得用于临床诊断或治疗, 不得用于食品或药品, 不得存放于普通住宅内 为了您的安全和健康, 请穿实验服并戴一次性手套操作 使用说明 : 1. 蛋白标准品的准备 a. 取 1.2ml 蛋白标准配制液加入到一管蛋白标准 (30mg BSA) 中, 充分溶解后配制成 25mg/ml 的蛋白标准溶液 配制后可立即使用, 也可以 -20ºC 长期保存 b. 取适量 25mg/ml 蛋白标准, 稀释至终浓度为 0.5mg/ml 例如取 20µl 25mg/ml 蛋白标准, 加入 980µl 稀释液即可配制成 0.5mg/ml 蛋白标准 蛋白样品在什么溶液中, 标准品也宜用什么溶液稀释 但是为了简便起见, 也可以用 0.9% NaCl 或 PBS 稀释标准品 稀释后的 0.5mg/ml 蛋白标准可以 -20ºC 长期保存 2. BCA 工作液配制根据样品数量, 按 50 体积 BCA 试剂 A 加 1 体积 BCA 试剂 B(50:1) 配制适量 BCA 工作液, 充分混匀 例如 5ml BCA 试剂 A 加 100µl BCA 试剂 B, 混匀, 配制成 5.1ml BCA 工作液 BCA 工作液室温 24 小时内稳定 3. 蛋白浓度测定 a. 将标准品按 µl 加到 96 孔板的标准品孔中, 加标准品稀释液补足到 20µl, 相当于标准品浓度分别为 mg/ml

2 b. 加适当体积样品到 96 孔板的样品孔中 如果样品不足 20µl, 需加标准品稀释液补足到 20µl 请注意记录样品体积 c. 各孔加入 200µl BCA 工作液,37ºC 放置 分钟 注 : 也可以室温放置 2 小时, 或 60ºC 放置 30 分钟 BCA 法测定蛋白浓度时, 颜色会随着时间的延长不断加深 并且显色反应会因温度升高而加快 如果浓度较低, 适合在较高温度孵育, 或适当延长孵育时间 d. 用酶标仪测定 A562, 或 nm 之间的其他波长的吸光度 e. 根据标准曲线和使用的样品体积计算出样品的蛋白浓度 常见问题 : 1. 测定标准曲线时发现随着标准品浓度的增加吸光度或颜色没有明显变化 可能的原因是样品中含有严重干扰 BCA 法测定蛋白浓度的物质, 详细的 BCA 法的兼容性列表请参考碧云天如下网页 : Chart For BCA Kit.pdf 2. 是否每次测定时都需要做标准曲线? 建议每次测定时都做标准曲线 因为 BCA 法测定时颜色会随着时间的延长不断加深, 并且显色反应的速度和温度有关, 所以除非精确控制显色反应的时间和温度, 否则如需精确测定宜每次都做标准曲线 相关产品 : 产品编号 产品名称 包装 P0006 Bradford 蛋白浓度测定试剂盒 1000 次 P0006C Bradford 蛋白浓度测定试剂盒 ( 去垢剂兼容型 ) 800 次 P0007 蛋白标准 (5mg/ml BSA) 1ml P0009 BCA 蛋白浓度测定试剂盒 ( 增强型 ) 5000 次 P0010 BCA 蛋白浓度测定试剂盒 ( 增强型 ) 500 次 P0010S BCA 蛋白浓度测定试剂盒 ( 增强型 ) 200 次 P0011 BCA 蛋白浓度测定试剂盒 5000 次 P0012 BCA 蛋白浓度测定试剂盒 500 次 P0012S BCA 蛋白浓度测定试剂盒 200 次 使用本产品的文献 : 1. Liu MJ, Wang Z, Li HX, Wu RC, Liu YZ, Wu QY. Mitochondrial dysfunction as an early event in the process of apoptosis induced by woodfordin I in human leukemia K562 cells. Toxicol Appl Pharmacol Jan 15;194(2): Zheng CH, Gao JQ, Zhang YP, Liang WQ. A protein delivery system: biodegradable alginate-chitosan-poly(lactic-co-glycolic acid) composite microspheres. Biochem Biophys Res Commun Oct 29; 323(4): Wu RC, Wang Z,Liu MJ,Chen DF,Yue XS. Yue. β 2-integrins mediate a novel form ofchemoresistance in cycloheximide-induced U937 apoptosis. Cell. Mol. Life Sci. 2004;61(16): Liu MJ, Yue PY, Wang Z, Wong RN. Methyl protodioscin induces G2/M arrest and apoptosis in K562 cells with the hyperpolarization of mitochondria.cancer Lett. 2005;224: Wu ZQ, Li M, Chen J, Chi ZQ, Liu JG. Involvement of camp/camp- dependent protein kinase signaling pathway in regulation of Na+,K+-ATPase upon activation of opioid receptors by morphine. Mol Pharmacol Mar;69(3): Yi ZC, Liu YZ, Li HX, Yin Y, Zhuang FY, Fan YB, Wang Z. Tellimagrandin I enhances gap junctional communication and attenuates the tumor phenotype of human cervical carcinoma HeLa cells in vitro. Cancer Lett Oct 8;242(1): Ma JF, Guo JL, Peng LW, Chen CY, Zhang LX. Decrease of photosystem II photochemistry in Arabidopsis ppt1 mutant is dependent on leaf age. J Integr Plant Biol.2006;48(12): Huang YY, Deng JY, Gu J, Zhang ZP, Maxwell A, Bi LJ, Chen YY, Zhou YF, Yu ZN, Zhang XE. The key DNA-binding residues in the C-terminal domain of Mycobacterium tuberculosis DNA gyrase A subunit (GyrA). Nucleic Acids Res. 2006;34(19): Wu ZQ, Chen J, Chi ZQ, Liu JG. Involvement of dopamine system in regulation of Na+,K+-ATPase in the striatum upon activation of opioid receptors by morphine. Mol Pharmacol Feb;71(2): Yi Z, Wang H, Zhang G, Xia B. Down regulation of connexin 43 in nasopharyngeal carcinoma cells is related to promoter methylation. Oral Oncol Oct;43(9): Dong S, Li C, Wu P, Tsien JZ, Hu Y. Environment enrichment rescues the neurodegenerative phenotypes in presenilins-deficient mice. Eur J Neurosci Jul;26(1): Wang Q, Liang C, Song J, Chen X. HA2 from the Helicoverpa armigera nucleopolyhedrovirus: a WASP-related protein that activates Arp2/3-induced actin filament formation. Virus Res Jul; 127(1): Pan YQ, Ding D, Li DX, Chen SQ. Expression and bioactivity analysis of Staphylococcal enterotoxin M and N. Protein Expr Purif Dec;56(2): Wang YL, Wang CY, Zhang BJ, Zhang ZZ. Shenfu injection suppresses apoptosis by regulation of Bcl-2 and caspase-3 during hypoxia/ reoxygenation in neonatal rat cardiomyocytes in vitro. Mol Biol Rep Feb;36(2): Nie H, Li Z, Lukas RJ, Shen Y, Song L, Wang X, Yin M. Construction of SH-EP1-alpha4beta2-hAPP695 Cell Line and Effects of Nicotinic Agonists on beta-amyloid in the Cells. Cell Mol Neurobiol Jan;28(1): Zhang H, Deng JY, Bi LJ, Zhou YF, Zhang ZP, Zhang CG, Zhang Y, Zhang XE. Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase. FEBS J Feb;275(4): Liu C,Wang Q, Guo H, Xia M, Yuan Q, Hu Y, Zhu H, Hou M, Ma J, Tang Z, Ling W. Plasma S-adenosylhomocysteine is a better biomarker of atherosclerosis than homocysteine in apolipoprotein E-deficient mice fed high dietary methionine. J Nutr Feb;138(2): hang GG, Shi RZ, Jiang DJ, Chen YR, Jia-Chen, Tang ZY, Bai YP, Xiao HB, Li YJ. Involvement of the endothelial DDAH/ADMA pathway in nitroglycerin tolerance: the role of ALDH-2. Life Sci Mar 26; 82(13-14): Fu Y, Zhang Z, Zhang G, Liu Y, Cao Y, Yu J, Hu J, Yin X. Adenovirus-mediated gene transfer of tissue factor pathway inhibitor induces apoptosis in vascular smooth muscle cells. Apoptosis May; 13(5): Tian B, Wang Z, Zhao Y, Wang D, Li Y, Ma L, Li X, Li J, Xiao N, Tian J, Rodriguez R. Effects of curcumin on bladder cancer cells and development of urothelial tumors in a rat bladder carcinogenesis model. Cancer Lett Jun 18;264(2): He X, Zhang Z, Zhang H, Zhao Y, Chai Z. Neurotoxicological evaluation of longterm lanthanum chloride exposure in rats. Toxicol Sci Jun; 103(2): Wan F, You J, Sun Y, Zhang XG, Cui FD, Du YZ, Yuan H, Hu FQ. Studies on PEG-modified SLNs loading vinorelbine bitartrate (I): preparation and evaluation in vitro. Int J Pharm Jul 9;359(1-2): Chen M, Li Y, Yang T, Wang Y, Bai Y, Xie X. ADMA induces monocyte adhesion via activation of chemokine receptors in cultured THP-1 cells. Cytokine Aug;43(2): Hu X, Zhou J, Zhang N, Tan H, Gao C. Preparation and properties of an injectable scaffold of poly(lactic-co-glycolic acid) microparticles/ chitosan hydrogel. J Mech Behav Biomed Mater Oct;1(4): Zhu M, Gu F, Shi J, Hu J, Hu Y, Zhao Z. Increased oxidative stress and astrogliosis responses in conditional double-knockout mice of Alzheimer-like presenilin-1 and presenilin-2. Free Radic Biol Med Nov 15;45(10): Li W, Liu Y, Zhang JW, Ai CZ, Xiang N, Liu HX, Yang L. Anti-androgenindependent prostate cancer effects of ginsenoside metabolites in vitro: mechanism and possible structure-activity relationship investigation. Arch Pharm Res Jan;32(1): Yuan Q, Li PD, Li BH, Yang XZ, Xu SB, Liu XH, Zhou FX, Zhang WJ.Differential IL-4/Stat6 activities correlate with differential expression of regulatory genes SOCS-1, SHP-1, and PP2A in colon cancer cells. J Cancer Res Clin Oncol / 8 P0011 BCA 蛋白浓度测定试剂盒 / 碧云天 /Beyotime

3 Jan;135(1): Liu XS, Jiang J, Jiao XY, Wu YE, Lin JH, Cai YM. Lycorine induces apoptosis and down-regulation of Mcl-1 in human leukemia cells. Cancer Lett Feb 8;274(1): Zhang LF, Peng SQ, Wang S. Decreased aortic contractile reaction to 5-hydroxytryptamine in rats with long-term hypertension induced by lead (Pb(2+)) exposure. Toxicol Lett Apr 25;186(2): Dong C, Wang Y, Zhu YZ. Asymmetric synthesis and biological evaluation of Danshensu derivatives as anti-myocardial ischemia drug candidates. Bioorg Med Chem May 1;17(9): Xiao X, Liu A, Wen H, Tian Y, Ni J, Liu G. Expression and localization of transcription factor Ets-1 in the rat ovary during the estrous cycle and pregnancy. Fertil Steril May;91(5 Suppl): Cheng B, Song J, Zou Y, Wang Q, Lei Y, Zhu C, Hu C. Responses of vascular smooth muscle cells to estrogen are dependent on balance between ERK and p38 MAPK pathway activities. Int J Cardiol May 29;134(3): Ding D, Huang P, Sun H, Xue Q, Pan Y, Chen S. Identification of protein components and quantitative immunoassay for SEC2 in staphylococcin injection. J Pharm Biomed Anal Aug 15;50(1): Liu X, Shi S, Ye J, Liu L, Sun M, Wang C. Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappaB/COX-2 activation and apoptosis in HaCaT cells. J Photochem Photobiol B Aug 3;96(2): Ma K, Hu M, Qi Y, Qiu L, Jin Y, Yu J, Li B. Structure-transfection activity relationships with glucocorticoid-polyethyl-enimine conjugate nuclear gene delivery systems. Biomaterials Aug; 30(22): Liao Z, Huang C, Zhou F, Xiong J, Bao J, Zhang H, Sun W, Xie C, Zhou Y.Radiation enhances suicide gene therapy in radioresistant laryngeal squamous cell carcinoma via activation of a tumor-specific promoter. Cancer Lett Sep 28;283(1): Chen Y, Wang J, Yao Y, Yuan W, Kong M, Lin Y, Geng D, Nie R. CRP regulates the expression and activity of tissue factor as well as tissue factor pathway inhibitor via NF-kappaB and ERK 1/2 MAPK pathway. FEBS Lett Sep 3;583(17): Chen WL, Qian Y, Meng WF, Pang JY, Lin YC, Guan YY, Chen SP, Liu J, Pei Z, Wang GL. A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in vitro. Biochem Pharmacol Oct 15;78(8): Lin Y, Chen Y, Yang X, Xu D, Liang S. Proteome analysis of a single zebrafish embryo using three different digestion strategies coupled with liquid chromatography-tandem mass spectrometry. Anal Biochem Nov 15;394(2): Xu Y, Ge R, Du J, Xin H, Yi T, Sheng J, Wang Y, Ling C. Corosolic acid induces apoptosis through mitochondrial pathway and caspase activation in human cervix adenocarcinoma HeLa cells. Cancer Lett Nov 1; 284(2): Chen S, Chen Y, Hu C, Jing H, Cao Y, Liu X. Association of clinicopathological features with UbcH10 expression in colorectal cancer. J Cancer Res Clin Oncol Mar;136(3): Yuan D, Pan Y, Zhang J, Shao C. Role of nuclear factor-kappab and P53 in radioadaptive response in Chang live cells. Mutat Res Jun 1;688(1-2): Li F, Gong QH, Wu Q, Lu YF, Shi JS. Icariin isolated from Epimedium brevicornum Maxim attenuates learning and memory deficits induced by d-galactose in rats. Pharmacol Biochem Behav. 2010;96(3): Wu GJ, Zhou LZ, Wang KW, Chen F, Sun Y, Duan YR, Zhu YJ, Gu HC.Hydroxylapatite nanorods: An efficient and promising carrier for gene transfection. J Colloid Interface Sci May 15;345(2): Lin Y, Xu J, Cao L, Han Y, Gao J, Xie N, Zhao X, Jiang H, Chi Z. Mitochondrial base excision repair pathway failed to respond to status epilepticus induced by pilocarpine. Neurosci Lett. 2010;474(1): Zhao L, Wang X, Chang Q, Xu J, Huang Y, Guo Q, Zhang S, Wang W, Chen X, Wang J. Neferine, a bisbenzylisoquinline alkaloid attenuates bleomycin-induced pulmonary fibrosis. Eur J Pharmacol. 2010; 627(1-3): Yan J, Sun XB, Wang HQ, Zhao H, Zhao XY, Xu YX, Guo JC, Zhu CQ. Chronic restraint stress alters the expression and distribution of phosphorylated tau and MAP2 in cortex and hippocampus of rat brain. Brain Res. 2010;1347: FY 48. Yuan H, Zhang W, Du YZ, Hu FQ. Ternary nanoparticles of anionic lipid nanoparticles/protamine/dna for gene delivery. Int J Pharm. 2010; 392(1-2): Huang LH, Hu JQ, Tao WQ, Li YH, Li GM, Xie PY, Liu XS, Jiang J. Gossypol inhibits phosphorylation of Bcl-2 in human leukemia HL-60 cells. Eur J Pharmacol. 2010;645(1-3): Cao BY, Yang YP, Luo WF, Mao CJ, Han R, Sun X, Cheng J, Liu CF. Paeoniflorin, a potent natural compound, protects PC12 cells from MPP+ and acidic damage via autophagic pathway. J Ethnopharmacol. 2010; 131(1): Li C, Xing C, Dong M, Zhou L, Li J, Wang G, Zou D, Wang R, Liu J, Niu Y. Beta-asarone protection against beta-amyloid-induced neurotoxicity in PC12 cells via JNK signaling and modulation of Bcl-2 family proteins. Eur J Pharmacol Jun 10;635(1-3): Baohua Y, Li L. Effects of SIRT6 silencing on collagen metabolism in human dermal fibroblasts. Cell Biol Int Jan;36(1): Han X, Lu M, Wang S, Lv D, Liu H. Targeting IKK/NF-κB pathway reduces infiltration of inflammatory cells and apoptosis after spinalcord injury in rats. Neurosci Lett Mar 5;511(1): Xiao O, Xie ZL, Lin BW, Yin XF, Pi RB, Zhou SY. Minocycline inhibits alkali burn-induced corneal neovascularization in mice. PLoS One. 2012;7(7):e Li TT, Zhang YS, He L, Liu B, Shi RZ, Zhang GG, Peng J. Inhibiton of vascular peroxidase alleviates cardiac dysfunction and apoptosis induced by ischemia-reperfusion. Can J Physiol Pharmacol Jul; 90(7): Jiang L, Lin J, Han H, Dong H, Zhao Q, Zhu S, Huang B. Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1). PLoS One. 2012;7(7):e Zhao P, Han T, Guo JJ, Zhu SL, Wang J, Ao F, Jing MZ, She YL, Wu ZH, Ye LB.HCV NS4B induces apoptosis through the mitochondrial death pathway. Virus Res Oct;169(1): Zhang Y, Xu Y, Zhu Q, Zhao F, Luo J, Zhang X, Wang X. Upregulation of dysbindin in temporal lobe epileptic foci of human and experimental animals. Synapse Jul;66(7): Zhao XY, Zhu YJ, Chen F, Wu J. Calcium phosphate nanocarriers dual-loaded with bovine serum albumin and ibuprofen: facile synthesis, sequential drug loading and sustained drug release. Chem Asian J Jun;7(7): Yang L, Cheng F, Liu T, Lu JR, Song K, Jiang L, Wu S, Guo W. Comparison of mesenchymal stem cells released from poly(n- sopropylacrylamide) copolymerfilm and by trypsinization. Biomed Mater Jun;7(3): Wang T, Fu F, Han B, Zhang L, Zhang X. Danshensu ameliorates the cognitive decline in streptozotocin-induced diabetic mice byattenuating advanced glycation end product-mediated neuroinflammation. J Neuroimmunol Apr;245(1-2): Zhang Y, Zhou L, Zhang X, Bai J, Shi M, Zhao G. Ginsenoside-Rd attenuates TRPM7 and ASIC1a but promotes ASIC2a expression in rats after focalcerebral ischemia. Neurol Sci Oct;33(5): Bi WY, Fu BD, Shen HQ, Wei Q, Zhang C, Song Z, Qin QQ, Li HP, Lv S, Wu SC, Yi PF, Wei XB. Sulfated Derivative of 20(S)-Ginsenoside Rh2 Inhibits Inflammatory Cytokines Through MAPKs andnf-kappa B Pathways in LPS-Induced RAW264.7 Macrophages. Inflammation Oct;35(5): Fauzee NJ, Wang YL, Dong Z, Li QG, Wang T, Mandarry MT, Xu L, Pan J. Novel hydrophilic docetaxel (CQMU-0519) analogue inhibits proliferation and induces apoptosis inhuman A549 lung, SKVO3 ovarian and MCF7 breast carcinoma cell lines. Cell Prolif Aug; 45(4): Lin N, Chen H, Zhang H, Wan X, Su Q. Mitochondrial reactive oxygen species (ROS) inhibition ameliorates palmitate-induced INS-1 betacell death. Endocrine Aug;42(1): Liu P, Shi Y, Yang Y, Cao Y, Shi Y, Li H, Liu J, Lin J, Jin Y. Schistosoma Japonicum UDP-Glucose 4-Epimerase Protein Is Located on the Tegument andinduces Moderate Protection against Challenge Infection. PLoS One. 2012;7(7):e Song Z, Li Y, Liu Y, Xin J, Zou X, Sun W. α-enolase, an Adhesion-Related Factor of Mycoplasma bovis. PLoS One. 2012; 7(6):e Ding Y, Huang X, Liu T, Fu Y, Tan Z, Zheng H, Zhou T, Dai J, Xu W. The Plasmodium Circumsporozoite Protein, a Novel NF-κB Inhibitor, Suppresses the Growth of SW480. Pathol Oncol Res Oct;18(4): Hao M, Kuang HY, Fu Z, Gao XY, Liu Y, Deng W. Exenatide prevents high-glucose-induced damage of retinal ganglion cells through a mitochondrial mechanism. Neurochem Int Jul;61(1): Zhao S, Zhao G, Xie H, Huang Y, Hou Y. A conjugate of an anti-midkine single-chain variable fragment to doxorubicin inhibits tumorgrowth. Braz J Med Biol Res Mar;45(3): Zhong Y, Liu T, Guo Z. Curcumin inhibits ox-ldl-induced MCP-1 expression by suppressing the p38mapk and NF-κBpathways in rat vascular smooth muscle cells. Inflamm Res Jan;61(1): Liu M, Zhou G, Song W, Li P, Liu H, Niu X, Fan Y. Effect of nano-hydroxyapatite on the axonal guidance growth of rat cortical neurons. Nanoscale May 21;4(10): Zhang L, Jiang G, Yao F, He Y, Liang G, Zhang Y, Hu B, Wu Y, Li Y, Liu H.Growth inhibition and apoptosis induced by osthole, a natural coumarin, in hepatocellularcarcinoma. PLoS One. 2012;7(5):e Liu H, Wu Z, Shi X, Li W, Liu C, Wang D, Ye X, Liu L, Na J, Cheng H, Chen L.A typical PKC, regulated by Rho GTPases and Mek/Erk, phosphorylates Ezrin during eight-cellembryocompaction. Dev Biol Mar 1;375(1): Yue Y, Yang X, Wei X, Chen J, Fu N, Fu Y, Ba K, Li G, Yao Y, Liang C, Zhang J, Cai X, Wang M. Osteogenic differentiation of adipose-derived stem cells prompted by low-intensity pulsed ultrasound. Cell Prolif Jun;46(3): Yin Y, Liu W, Ji G, Dai Y. The essential role of p38 MAPK in mediating the interplay of oxldl and IL-10 in regulating endothelial cellapoptosis. Eur J Cell Biol Apr-May;92(4-5): Liu X, Wang M, Chen H, Guo Y, Ma F, Shi F, Bi Y, Li Y. Hypothermia protects the brain from transient global ischemia/reperfusion by attenuating endoplasmic reticulum response-induced apoptosis through CHOP. PLoS One. 2013;8(1):e Qin D, Li K, Qu J, Wang S, Zou C, Sheng Y, Huang A, Tang H. HBx and HBs regulate RhoC expression by upregulating transcription factor Ets-1. Arch Virol Aug;158(8): Jia F, Fan J, Zhang B, Yuan Z. Mutagenesis of D80-82 and G83 residues in West Nile Virus NS2B: effects on NS2B-NS3 activity and viralreplication.virol Sin. 碧云天 /Beyotime / P0011 BCA 蛋白浓度测定试剂盒 3 / 8

4 2013 Feb;28(1): Li X, Wang X, Han C, Wang X, Xing G, Zhou L, Li G, Niu Y. Astragaloside IV suppresses collagen production of activated hepatic stellate cells via oxidative stress-mediatedp38 MAPK pathway. Free Radic Biol Med Jul;60: Jin R, Zhu W, Cao S, Chen R, Jin H, Liu Y, Wang S, Wang W, Xiao G. Japanese encephalitis virus activates autophagy as a viral immune evasion strategy. PLoS One. 2013;8(1):e Wang Y, Li B, Li Z, Huang S, Wang J, Sun R. Improvement of hypoxia-ischemiainduced white matter injury in immature rat brain by ethyl pyruvate. Neurochem Res Apr;38(4): Yan Y, Liu H, Wen H, Jiang X, Cao X, Zhang G, Liu G. The novel estrogen receptor GPER regulates the migration and invasion of ovarian cancer cells. Mol Cell Biochem Jun;378(1-2): Chen Y, Huang C, Bai C, Gao H, Ma R, Liu X, Dong Q. Benzo[α]pyrene repressed DNA mismatch repair in human breast cancer cells. Toxicology Feb 8;304: Zou M, Xia S, Zhuang L, Han N, Chu Q, Chao T, Peng P, Chen Y, Gui Q, Yu S.Knockdown of the Bcl-2 gene increases sensitivity to EGFR tyrosine kinase inhibitors in the H1975 lung cancercell line harboring T790M mutation. Int J Oncol Jun;42(6): Li L, Ye XP, Lu AZ, Zhou SQ, Liu H, Liu ZJ, Jiang S, Xu SY. Hyperglycemia magnifies bupivacaine-induced cell apoptosis triggered by mitochondria dysfunction andendoplasmic reticulum stress. J Neurosci Res Jun; 91(6): Zhang H, Wang ZW, Wu HB, Li Z, Li LC, Hu XP, Ren ZL, Li BJ, Hu ZP.Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cellsvia ROS-dependent ERK-NF-κB pathways. Mol Cell Biochem Mar;375(1-2): Yan T, Liu Y, Cui K, Hu B, Wang F, Zou L. MiR-126 regulates EPCs function: implications for a role of mir-126 in preeclampsia. J Cell Biochem.2013 Sep;114(9): Bi LQ, Zhu R, Kong H, Wu SL, Li N, Zuo XR, Zhou SM, Kou JP, Yu BY, Wang H, Xie WP. 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