PCR 条形码试剂盒方案器材和耗材 Oxford NANOPORE Technologies Oxford Nanopore Technologies Oxford Science Park, Oxford OX4 4GA, UK

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1 PCR 条形码试剂盒方案器材和耗材 Nanopore Technologies Science Park, OX4 4GA, UK

2 PCR 条形码试剂盒的器材和耗材 PCR 条形码基因组 DNA 英文器材 Hula mixer (gentle rotator mixer) Hula 混匀仪 ( 低速旋转式混匀仪 ) Magnetic rack for magnetic bead separation in 1.5 ml tubes Microfuge Vortex mixer Heating block at 37 C capable of taking 1.5 ml tubes Thermal cycler at 20 C and 65 C P2 pipette Ice bucket with ice Pre-chilled freezer block at -20 C for 200 µl tubes Eppendorf tube marker pen Timer 中文磁力架, 用于 1.5ml 管中的磁珠分离低速离心机旋涡混匀仪 37 微量恒温仪, 可放置 1.5 ml 管 20 和 65 热循环仪 P2 移液枪装有冰的冰桶 -20 下预冷的冷冻砖, 用于 200 µl 管 Eppendorf 管记号笔计时器第三方耗材 Agencourt AMPure XP beads Agencourt AMPure XP 磁珠 NEBNext End repair / da-tailing Module NEB Blunt/TA Ligase Master Mix MyOne C1 Streptavidin beads NEB Next FFPE Repair Mix Covaris g-tube NEBNext 末端修复 / da 尾添加模块 NEB 平末端 / TA 连接酶预混液 MyOne C1 链霉亲和素磁珠 NEB Next FFPE 修复混合液 Covaris g-tube 耗材 Nanopore Sequencing Kit 纳米孔测序试剂盒 PCR Barcoding Kit (EXP-PBC001) PCR 条形码试剂盒 (EXP-PBC001) 1.5 ml Eppendorf DNA LoBind tubes 1.5ml Eppendorf DNA LoBind 离心管 0.2 ml thin-walled PCR tubes 0.2ml 薄壁 PCR 管 Nuclease-free water Freshly prepared 70% ethanol in nuclease-free water P1000 / P200 / P100 / P20 / P10 / P2 pipette tips 无核酸酶水新制备的 70% 乙醇 ( 用无核酸酶水配制 ) P1000 / P200 / P100 / P20 / P10 / P2 移液枪枪头本文件及其内容版权归 Nanopore Technologies 所有版权所有 * Nanopore Technologies wheel GridION 和 MinION 均为 Nanopore 2

4 PCR 条形码扩增子器材 Hula mixer (gentle rotator mixer) Hula 混匀仪 ( 低速旋转式混匀仪 ) Magnetic rack for magnetic bead separation in 1.5 ml tubes Microfuge Vortex mixer Heating block at 37 C capable of taking 1.5 ml tubes Thermal cycler at 20 C and 65 C P2 pipette Ice bucket with ice Pre-chilled freezer block at -20 C for 200 µl tubes Eppendorf tube marker pen Timer 磁力架, 用于 1.5ml 管中的磁珠分离低速离心机旋涡混匀仪 37 微量恒温仪, 可放置 1.5 ml 管 20 和 65 热循环仪 P2 移液枪装有冰的冰桶 -20 下预冷的冷冻砖, 用于 200 µl 管 Eppendorf 管记号笔计时器第三方耗材 Agencourt AMPure XP beads Agencourt AMPure XP 磁珠 NEBNext End repair / da-tailing Module NEB Blunt/TA Ligase Master Mix MyOne C1 Streptavidin beads LongAmp Taq 2X Master Mix (e.g. NEB NEBNext 末端修复模块 / da 尾添加模块 NEB 平末端 / TA 连接酶预混液 MyOne C1 链霉亲和素磁珠 LongAmp Taq 2X 预混液 ( 例如 :NEB 耗材 Nanopore Sequencing Kit 纳米孔测序试剂盒 PCR Barcoding Kit (EXP-PBC001) PCR 条形码试剂盒 (EXP-PBC001) 1.5 ml Eppendorf DNA LoBind tubes 1.5ml Eppendorf DNA LoBind 离心管 0.2 ml thin-walled PCR tubes 0.2ml 薄壁 PCR 管 Nuclease-free water Freshly prepared 70% ethanol in nuclease-free water P1000 / P200 / P100 / P20 / P10 / P2 pipette tips 无核酸酶水如果使用 96 型 PCR 条形码试剂盒, 则还需要 0.2 ml 96 孔 PCR 板新制备的 70% 乙醇 ( 用无核酸酶水配制 ) P1000 / P200 / P100 / P20 / P10 / P2 移液枪枪头本文件及其内容版权归 Nanopore Technologies 所有版权所有 * Nanopore Technologies wheel GridION 和 MinION 均为 Nanopore 4

5 PCR 条形码 cdna 器材 Hula mixer (gentle rotator mixer) Hula 混匀仪 ( 低速旋转式混匀仪 ) Magnetic rack for magnetic bead separation in 1.5 ml tubes Microfuge Vortex mixer Heating block at 37 C capable of taking 1.5 ml tubes Thermal cycler at 20 C and 65 C P2 pipette Ice bucket with ice Pre-chilled freezer block at -20 C for 200 µl tubes Eppendorf tube marker pen Timer 磁力架, 用于 1.5ml 管中的磁珠分离低速离心机旋涡混匀仪 37 微量恒温仪, 可放置 1.5 ml 管 20 和 65 热循环仪 P2 移液枪装有冰的冰桶 -20 下预冷的冷冻砖, 用于 200 µl 管 Eppendorf 管记号笔计时器第三方耗材 Agencourt AMPure XP beads Agencourt AMPure XP 磁珠 NEBNext End repair / da-tailing Module NEB Blunt/TA Ligase Master Mix MyOne C1 Streptavidin beads NEBNext 末端修复 / da 尾添加模块 NEB 平末端 / TA 连接酶预混液 MyOne C1 链霉亲和素磁珠 SuperScript II reverse transcriptase, 5x SuperScript II 逆转录酶 5x 第一链缓 first strand buffer and 100 mm DTT (Life 冲液和 100 mm 二硫苏糖醇 (DTT) Technologies ) 溶液 (Life Technologies ) 10 mm dntp solution (e.g. NEB N0447) 10mM 脱氧核糖核苷三磷酸 (dntp) 溶液 ( 例如 :NEB N0447) Second strand synthesis kit (e.g. NEB E6111) LongAmp Taq 2X Master Mix (e.g. NEB Oligos for reverse transcription polyt-vn RT primer (PCR-VN), usersupplied Strand-switching oligo (PCR_Sw_mod_3G), user supplied 第二链合成试剂盒 ( 例如 :NEB E6111) LongAmp Taq 2X 预混液 ( 例如 :NEB 用于反转录的寡核苷酸 polyt-vn RT 引物 (PCR-VN), 由用户提供链转换寡核苷酸 (PCR_Sw_mod_3G), 由用户提供耗材 Nanopore sequencing kit 纳米孔测序试剂盒 PCR Barcoding Kit (EXP-PBC001) PCR 条形码试剂盒 (EXP-PBC001) 本文件及其内容版权归 Nanopore Technologies 所有版权所有 * Nanopore Technologies wheel GridION 和 MinION 均为 Nanopore 5

6 ng PolyA+ RNA ng PolyA+ RNA 1.5 ml Eppendorf DNA LoBind tubes 1.5ml Eppendorf DNA LoBind 离心管 0.2 ml thin-walled PCR tubes 0.2ml 薄壁 PCR 管 Nuclease-free water 无核酸酶水 10 mm Tris-HCl ph mm 盐酸盐,pH=8.5 Freshly prepared 70% ethanol in nuclease-free water P1000 / P200 / P100 / P20 / P10 / P2 pipette tips 如果使用 96 型 PCR 条形码试剂盒, 则还需要 0.2 ml 96 孔 PCR 板新制备的 70% 乙醇 ( 用无核酸酶水配制 ) P1000 / P200 / P100 / P20 / P10 / P2 移液枪枪头本文件及其内容版权归 Nanopore Technologies 所有版权所有 * Nanopore Technologies wheel GridION 和 MinION 均为 Nanopore 6

2004级研究生现代免疫学实验技术结业报告

2004级研究生现代免疫学实验技术结业报告 PCR PCR PCR RT-PCR PCR (Polymerase Chain Reaction,PCR) 80 DNA, DNA PCR PCR PCR DNA PCR -- -- DNA DNA 93 DNA PCR DNA DNA ( ) DNA 55 DNA DNA -- TaqDNA dntp DNA -- -- PCR PCR DNA DNA Y (1 X) n Y DNA X (Y)