Western Substrate Get. Western lot 化学发光检测的明智之选 2012 io-rad Laboratories, Inc. ulletin 0000

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1 Imaging Western 化学发光底物 Western lot 化学发光检测的明智之选

2 Western Substrate Get. Western lot 化学发光检测的明智之选 2012 io-rad Laboratories, Inc. ulletin 0000

3 飞克级灵敏度兼容所有辣根过氧化物酶 HRP 标记二抗极低的非特异性背景超长发光时间理想的数字化和胶片检测试剂兼容 NC 膜 PVDF 膜及低荧光背景 PVDF 膜 24 小时信号持续时间允许多次曝光以优化检测超凡灵敏度常温保存一年有效期便捷灵活 western 化学发光底物可以有效替代多种级别底物它既可以使用胶片也可以使用数字成像仪进行检测并且适用于高表达和低表达 Pico Film 蛋白当您使用胶片成像时您将可以灵活控制曝光时间 Femto Dura PLUS Digital ImageR 简化您的选择可有效替代各种化学发光底物 io-rad Laboratories, Inc.

高灵敏度与其他化学发光底物相比具有出众的检测灵敏度独立机构将与 Thermo Scientific 的和及 GE 的进行的对比实验表明是市场上信噪比最好的化学发光底物 00:00 hr Purified DH, ng 10 5 2.

化学发光底物灵敏度对比, 5 种不同底物的化学发光结果样品同为纯化后的 DH 印迹按厂家推荐的方法进行孵育并在 ChemiDoc MP 系统上曝光 10 秒钟成像, 每个条带的信噪比柱状图很明显和的信噪比是最好的实验室测试

0 10.0 5.0 2.5 Total protein load, µg 图 2. 慕尼黑大学实验室使用不同化学发光底物进行盲测的结果对比大鼠 S1 组织蛋白上样量分别为 10 µg ( lane 1 ) 5 µg ( lane 2 ) 2.

4 高灵敏度与其他化学发光底物相比具有出众的检测灵敏度独立机构将与 Thermo Scientific 的和及 GE 的进行的对比实验表明是市场上信噪比最好的化学发光底物 00:00 hr Purified DH, ng Response, RU Protein per well, ng 图 1. 化学发光底物灵敏度对比, 5 种不同底物的化学发光结果样品同为纯化后的 DH 印迹按厂家推荐的方法进行孵育并在 ChemiDoc MP 系统上曝光 10 秒钟成像, 每个条带的信噪比柱状图很明显和的信噪比是最好的实验室测试灵敏度对比实验德国慕尼黑大学 Lab s Current Substrate () Sample () Sample () Intensity (Single/ackground) Total protein load, µg 图 2. 慕尼黑大学实验室使用不同化学发光底物进行盲测的结果对比大鼠 S1 组织蛋白上样量分别为 10 µg ( lane 1 ) 5 µg ( lane 2 ) 2.5 µg ( lane 3 ) 使用 10% Mini-PROTEN TGX 凝胶进行电泳和 Trans-lot 系统转印 7 分钟分别使用使用和化学发光进行印迹检测蛋白定量使用配有 Image Lab 的 ChemiDoc MP 系统及光密度法进行在所有的上样量测试中都是最灵敏度的检测试剂

5 超长发光时间底物的超长发光时间允许您使用胶片或 CCD 成像时可多次曝光以优化获取参数得到最佳结果 01:00 hr Purified DH, ng Response, RU Protein per well, ng 图 3. 不同化学发光底物的信号强度对比相同的 5 个印迹使用不同底物及其最优孵育条件得到的结果检测是在 1 个小时以后进行的并在 ChemiDoc MP 成像仪上曝光 30 秒, 以柱状图表示的各条带响应值和的信号强度最高其他的底物信号均在 1 个小时以后发生了明显的下降实验室评价信号持续时间测试使用 X-ray 胶片成像旧金山加州大学 00:00 min 00:40 min S S Sample () Sample () 65 kd 50 kd 65 kd 50 kd Lane S: MW standard Lane 1: No stimulation Lane 2: 5 min stimulation Lane 3: 15 min stimulation Lane 4: 30 min stimulation Lane 5: 45 min stimulation 50% 50% signal remains 96% 96% signal remains CD8 T cell stimulation with 50 ng/ml PM Primary antibody phospho-kt Ser 473 (Cell Signaling Technolgy, Inc.) Secondary antibody anti-rabbit HRP (Cell Signaling Technolgy, Inc.) 图 4. 在旧金山加州大学实验室进行的信号持续时间对比实验, 相同的样品在使用和底物孵育后立即进行胶片曝光并在 40 分钟后再进行一次曝光, 剩余信号强度比例比信号持续能力优异的多 2012 io-rad Laboratories, Inc. ulletin 0000 Western Substrate

6 订货信息货号描述化学发光底物 Western Substrate, 200 ml, contains 100 ml western peroxide reagent and 100 ml western luminol/ enhancer reagent Western Substrate, 500 ml, contains 250 ml western peroxide reagent and 250 ml western luminol/ enhancer reagent 蛋白标准品 Precision Plus Protein ll lue Standards, 500 ml, 50 applications Precision Plus Protein Unstained Standards, 1 ml, 100 applications Precision Plus Protein WesternC Pack, 50 applications 缓冲液 x Tris/Glycine/SDS, 1 L x Laemmli Sample uffer, 10 ml 电泳槽 Criterion Cell, includes electrophoresis buffer tank, lid with power cables, 3 sample loading guides (12+2-well, 18-well, 26-well) Mini-PROTEN Tetra Cell for Mini Precast Gels, 4-gel vertical electrophoresis system, includes electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam 电源 PowerPac asic Power Supply, / V PowerPac Universal Power Supply, / V 货号描述成像系统 ChemiDoc MP System, gel imaging system, PC or Mac, includes darkroom, UV transilluminator, epi-white illumination, camera, power supply, cables, Image Lab software Red LED Module Kit, includes 2 epi-red LED modules, 1 red emission filter Green LED Module Kit, includes 2 epi-green LED modules, 1 green emission filter lue LED Module Kit, includes 2 epi-blue LED modules, 1 blue emission filter 转印系统 Trans-lot Turbo Transfer Starter System, blotting instrument, includes base, 2 cassettes to hold 1 2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit Trans-lot Turbo 即用型转印包 Trans-lot Transfer Pack, mini, PVDF, pkg of Trans-lot Transfer Pack, midi, PVDF, pkg of Trans-lot Transfer Pack, mini, nitrocellulose, pkg of Trans-lot Transfer Pack, midi, nitrocellulose, pkg of 10 Trans-lot Turbo 待装配转印包 Trans-lot Turbo RT Transfer Kit, mini, nitrocellulose, for 40 blots Trans-lot Turbo RT Transfer Kit, midi, nitrocellulose, for 40 blots Trans-lot Turbo RT Transfer Kit, mini, PVDF, for 40 blots Trans-lot Turbo RT Transfer Kit, midi, PVDF, for 40 blots Trans-lot Turbo RT Transfer Kit, mini, LF PVDF, for 40 blots Trans-lot Turbo RT Transfer Kit, midi, LF PVDF, for 40 blots 10-Well 10-Well 12-Well 描述 30 μl 50 μl 20 μl Mini-PROTEN TGX 免染预制胶 10% % % ny kd 所有的货号都有两种包装 10 片装货号如上和 2 片装如果需要订购 2 片装请在相应货号后加注 S 12+2*-Well 18-Well 26-Well 描述 45 µl 30 µl 15 µl Criterion TGX 免染预制胶 ** 10% % % ny kd * 参比通道可以加 15 µl marker 或标准品 ** Criterion TGX 免染预制胶为单片包装我们也提供其他规格包装 SuperSignal is a trademark of Thermo Fisher Scientific. Precision Plus Protein standards are sold under license from Life Technologies Corporation, Carlsbad, C, for use only by the buyer of the product. The buyer is not authorized to sell or resell this product or its components. Mac is a trademark of pple Inc. 伯乐生命医学产品上海有限公司地址上海市浦东东方路 18 号保利广场 E 栋 3 楼电话传真邮编北京办事处地址北京朝阳区曙光西里 5 号栋凤凰置地广场 22 楼电话传真邮编广州办事处地址广州市环市东路 403 号广州国际电子大厦室电话传真邮编伯乐公司中国呼叫中心武汉办事处地址湖北省武汉市武昌区临江大道 96 号武汉万达中心室电话传真邮编欢迎访问 io-rad Laboratories, Inc. Life Science Group Web site US ustralia ustria elgium razil Canada China Shanghai eijing Toll-free Tech Support Czech Republic Denmark Finland France Germany Greece Hong Kong Hungary India Israel Italy Japan Korea Mexico The Netherlands New Zealand Norway Poland Portugal Russia Singapore South frica Spain Sweden Switzerland Taiwan Thailand United Kingdom ulletin 6305 Rev sia Pacific/China Printed in China Sig 1211

Protein Labeling and Detection

Protein Labeling and Detection ECL plex Product Specialist 1/ Western Blot 2/ Western Blotting- PI 3/ Western Blotting 1. 2 1 2. 3. 4. Y 3 4 5. 4/ 1. SDS- (SDS-PAGE) SDS ( β- ) --SDS 5/ SDS-PAGE - - - (Tris-HCL ph 6.8) glycine/chloride

Western Substrate Get. Western lot 化学发光检测的 明 智 之选 2012 io-rad Laboratories, Inc. ulletin 0000

Western Substrate Get. Western lot 化学发光检测的明智之选 2012 io-rad Laboratories, Inc. ulletin 0000