然后再加待测样品 10μl( 样品最终稀释度为 5 倍 ) 加样将样品加于酶标板孔底部, 尽量不触及孔壁, 轻轻晃动混匀 3. 温育 : 用封板膜封板后置 37 温育 30 分钟 4. 配液 : 将 30 倍浓缩洗涤液用蒸馏水 30 倍稀释后备用 5. 洗涤 : 小心揭掉封板膜, 弃去液体, 甩干,

Size: px
Start display at page:

Download "然后再加待测样品 10μl( 样品最终稀释度为 5 倍 ) 加样将样品加于酶标板孔底部, 尽量不触及孔壁, 轻轻晃动混匀 3. 温育 : 用封板膜封板后置 37 温育 30 分钟 4. 配液 : 将 30 倍浓缩洗涤液用蒸馏水 30 倍稀释后备用 5. 洗涤 : 小心揭掉封板膜, 弃去液体, 甩干,"

Transcription

1 大鼠胰岛素自身抗体 (IAA) 酶联免疫分析 试剂盒使用说明书 本试剂盒仅供研究使用 检测范围 : 96T 3 pmol/l -120 pmol/l 使用目的 : 本试剂盒用于测定大鼠血清 血浆及相关液体样本中胰岛素自身抗体 (IAA) 含量 实验原理 本试剂盒应用双抗原夹心法测定标本中大鼠胰岛素自身抗体 (IAA) 水平 用纯化的大 鼠胰岛素自身抗原包被微孔板, 制成固相抗原, 往包被单抗的微孔中依次加入胰岛素自身抗 体, 再与 HRP 标记的胰岛素自身抗原结合, 形成抗原 - 抗体 - 酶标抗原复合物, 经过彻底洗 涤后加底物 TMB 显色 TMB 在 HRP 酶的催化下转化成蓝色, 并在酸的作用下转化成最终 的黄色 颜色的深浅和样品中的胰岛素自身抗体 (IAA) 呈正相关 用酶标仪在 450nm 波 长下测定吸光度 (OD 值 ), 通过标准曲线计算样品中大鼠胰岛素自身抗体 (IAA) 浓度 试剂盒组成 1 30 倍浓缩洗涤液 20ml 1 瓶 7 终止液 6ml 1 瓶 2 酶标试剂 6ml 1 瓶 8 标准品 (240 pmol/l) 0.5ml 1 瓶 3 酶标包被板 12 孔 8 条 9 标准品稀释液 1.5ml 1 瓶 4 样品稀释液 6ml 1 瓶 10 说明书 1 份 5 显色剂 A 液 6ml 1 瓶 11 封板膜 2 张 6 显色剂 B 液 6ml 1/ 瓶 12 密封袋 1 个 标本要求 1. 标本采集后尽早进行提取, 提取按相关文献进行, 提取后应尽快进行实验 若不能 马上进行试验, 可将标本放于 -20 保存, 但应避免反复冻融 2. 不能检测含 NaN3 的样品, 因 NaN3 抑制辣根过氧化物酶的 (HRP) 活性 操作步骤 1. 标准品的稀释 : 本试剂盒提供原倍标准品一支, 用户可按照下列图表在小试管中进行稀 释 120 pmol/l 5 号标准品 150μl 的原倍标准品加入 150μl 标准品稀释液 60 pmol/l 4 号标准品 150μl 的 5 号标准品加入 150μl 标准品稀释液 30 pmol/l 3 号标准品 150μl 的 4 号标准品加入 150μl 标准品稀释液 15 pmol/l 2 号标准品 150μl 的 3 号标准品加入 150μl 标准品稀释液 7.5 pmol/l 1 号标准品 150μl 的 2 号标准品加入 150μl 标准品稀释液 2. 加样 : 分别设空白孔 ( 空白对照孔不加样品及酶标试剂, 其余各步操作相同 ) 标准孔 待测样品孔 在酶标包被板上标准品准确加样 50μl, 待测样品孔中先加样品稀释液 40μl,

2 然后再加待测样品 10μl( 样品最终稀释度为 5 倍 ) 加样将样品加于酶标板孔底部, 尽量不触及孔壁, 轻轻晃动混匀 3. 温育 : 用封板膜封板后置 37 温育 30 分钟 4. 配液 : 将 30 倍浓缩洗涤液用蒸馏水 30 倍稀释后备用 5. 洗涤 : 小心揭掉封板膜, 弃去液体, 甩干, 每孔加满洗涤液, 静置 30 秒后弃去, 如此重复 5 次, 拍干 6. 加酶 : 每孔加入酶标试剂 50μl, 空白孔除外 7. 温育 : 操作同 3 8. 洗涤 : 操作同 5 9. 显色 : 每孔先加入显色剂 A50μl, 再加入显色剂 B50μl, 轻轻震荡混匀,37 避光显色 15 分钟. 10. 终止 : 每孔加终止液 50μl, 终止反应 ( 此时蓝色立转黄色 ) 11. 测定 : 以空白空调零,450nm 波长依序测量各孔的吸光度 (OD 值 ) 测定应在加终止液后 15 分钟以内进行 操作程序总结 : 计算以标准物的浓度为横坐标,OD 值为纵坐标, 在坐标纸上绘出标准曲线, 根据样品的 OD 值由标准曲线查出相应的浓度 ; 再乘以稀释倍数 ; 或用标准物的浓度与 OD 值计算出标准曲线的直线回归方程式, 将样品的 OD 值代入方程式, 计算出样品浓度, 再乘以稀释倍数,

3 即为样品的实际浓度 注意事项 1. 试剂盒从冷藏环境中取出应在室温平衡 分钟后方可使用, 酶标包被板开封后如未用完, 板条应装入密封袋中保存 2. 浓洗涤液可能会有结晶析出, 稀释时可在水浴中加温助溶, 洗涤时不影响结果 3. 各步加样均应使用加样器, 并经常校对其准确性, 以避免试验误差 一次加样时间最好控制在 5 分钟内, 如标本数量多, 推荐使用排枪加样 4. 请每次测定的同时做标准曲线, 最好做复孔 如标本中待测物质含量过高 ( 样本 OD 值大于标准品孔第一孔的 OD 值 ), 请先用样品稀释液稀释一定倍数 (n 倍 ) 后再测定, 计算时请最后乘以总稀释倍数 ( n 5) 5. 封板膜只限一次性使用, 以避免交叉污染 6. 底物请避光保存 7. 严格按照说明书的操作进行, 试验结果判定必须以酶标仪读数为准. 8. 所有样品, 洗涤液和各种废弃物都应按传染物处理 9. 本试剂不同批号组分不得混用 10. 如与英文说明书有异, 以英文说明书为准 保存条件及有效期 1. 试剂盒保存 :; 有效期 :6 个月

4 RD Rat IAA FOR RESEARCH USE ONLY Assay range:3pmol/l - 120pmol/L 96 determinations Purpose This kit allows for the determination of IAA concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat IAA level in the sample,use Purified Rat IAA antibody to coat microtiter plate wells, make solid-phase antibody, then add IAA to wells, Combined IAA antibody which With HRP labeled goat anti-rat become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat IAA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Materials provided with the kit 1 wash solution 20ml 1bottle 7 Stopp Solution 6ml 1 bottle 2 HRP-Conjugate reagent 6ml 1 bottle 8 Standard( 240 pmol/l) 0.5ml 1 bottle 3 Microelisa stripplate 12well 8strips 9 Standard diluent 1.5ml 1bottle 4 Sample diluent 6ml 1 bottle 10 Instruction 1 5 Chromogen Solution A 6ml 1 bottle 11 Closure plate membrane 6 Chromogen Solution B 6ml 1 bottle 12 Sealed bags 1 Specimen requirements 2

5 1. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can t, specimen can be kept in -20 to preserve, Avoid repeated freeze-thaw cycles. 2. Can t detect the sample which contain NaN3, because NaN3 inhibits HRP active. Assay procedure 1. Dilute and add sample:dilute Original density Standard as follow table: 120pmol/L 5 Standard 150μl Original density Standard+150μl Standard diluent 60pmol/L 30pmol/L 15pmol/L 7.5pmol/L 4 Standard 150μl 5 Standard+150μl Standard diluent 3 Standard 150μl 4 Standard+150μl Standard diluent 2 Standard 150μl 3 Standard +150μl Standard diluent 1 Standard 150μl 2 Standard +150μl Standard diluent 2.add sample:set blank wells separately (blank comparison wells don t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells, don t touch the well wall as far as possible, and Gently mix. 3.Incubate: After closing plate with Closure plate membrane,incubate for 30 min at Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve. 5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat. 6.add enzyme:add HRP-Conjugate reagent 50μl to each well, except blank well. 7.incubate:Operation with 3. 8.washing:Operation with 5. 9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at Stop the reaction:add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color). 11.assay:take blank well as zero, Read absorbance at 450nm after Adding Stop Solution and

6 within 15min. Steps description Standard, Sample diluent Add Standard, Sample diluent, incubate for 30 min at 37. Wash 5 time,add HRP-Conjugate reagent, incubate for 30 min at 37. Wash 5 times,add Chromogen Solution A and B, incubate for 30 min at 37. Add Stopp Solution Read absorbance at 450nm within 15 min Calculate calculate Take the standard density as the horizontal, the OD value for the vertical,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line

7 regression equation of the standard curve with the standard density and the OD value,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density. Important notes 1. The kit takes out from the refrigeration environment should be balanced minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag. 2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute. Washing does not affect the result. 3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much, recommend to use Volley. 4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.( n 5). 5. Closure plate membrane only limits the disposable use, to avoid cross-contamination. 6. The substrate evade the light preservation. 7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard. 8. All samples, washing buffer and each kind of reject should according to infective material process. 9. Do not mix reagents with those from other lots. Storage and validity 1.Storage: validity: six months

<443A5CD7CAC1CF315CD7C0C3E65CB7A2BBF5D0C5CFA25C B7A2BBF55C362E39B7A2BBF55CBBA6B7E55CD0A1CAF3B0D7CFB8B0FBBDE9CBD839A3A8494C2D39A3A92E646F63>

<443A5CD7CAC1CF315CD7C0C3E65CB7A2BBF5D0C5CFA25C B7A2BBF55C362E39B7A2BBF55CBBA6B7E55CD0A1CAF3B0D7CFB8B0FBBDE9CBD839A3A8494C2D39A3A92E646F63> 小鼠白细胞介素 9(IL-9) 酶联免疫分析 试剂盒使用说明书 本试剂盒仅供研究使用 检测范围 : 96T 15ng/L-320ng/L 使用目的 : 本试剂盒用于测定小鼠血清 血浆及相关液体样本中鸡白细胞介素 -9(IL-9) 含量 实验原理本试剂盒应用双抗体夹心法测定标本中小鼠白细胞介素 -9(IL-9) 水平 用纯化的小鼠白细胞介素 -9(IL-9) 抗体包被微孔板, 制成固相抗体, 往包被单抗的微孔中依次加入白细胞介素

More information

人白细胞介素10(IL-10)酶联免疫分析(ELISA)

人白细胞介素10(IL-10)酶联免疫分析(ELISA) 大鼠白细胞介素 6(IL-6) 酶联免疫检测 试剂盒使用说明书 AE90247Ra 使用前仔细阅读本说明书 本酶联免疫试剂盒是基于双抗体夹心技术原理, 来检测大鼠白细胞介素 6(IL-6), 只能用于研究用途, 不得用于医学诊断 用 途 : 用于大鼠血清 血浆及相关液体样本中白细胞介素 6(IL-6) 测定 工作原理 本试剂盒采用的是生物素双抗体夹心酶联免疫吸附法 (ELISA) 测定样品中大鼠白细胞介素

More information

/ 分 ) 仔细收集上清 检测细胞内的成份时, 用 PBS(PH ) 稀释细胞悬液, 细胞浓度达到 100 万 /ml 左右 通过反复冻融, 以使细胞破坏并放出细胞内成份 离心 20 分钟左右 ( 转 / 分 ) 仔细收集上清 保存过程中如有沉淀形成, 应再次离心 5.

/ 分 ) 仔细收集上清 检测细胞内的成份时, 用 PBS(PH ) 稀释细胞悬液, 细胞浓度达到 100 万 /ml 左右 通过反复冻融, 以使细胞破坏并放出细胞内成份 离心 20 分钟左右 ( 转 / 分 ) 仔细收集上清 保存过程中如有沉淀形成, 应再次离心 5. 大鼠胰岛素自身抗体 (IAA) 酶联免疫分析 (ELISA) 试剂盒使用说明书 本试剂仅供研究使用目的 : 本试剂盒用于测定大鼠血清 血浆 组织等样本中胰岛素自身抗体 (IAA) 的含量 实验原理 : 本试剂盒应用间接法测定标本中大鼠胰岛素自身抗体 (IAA) 水平 用纯化的大鼠胰岛素自身抗体 (IAA) 捕获抗原包被微孔板, 制成固相抗原, 往包被的微孔中依次加入大鼠胰岛素自身抗体 (IAA),

More information

人白细胞介素10(IL-10)酶联免疫分析(ELISA)

人白细胞介素10(IL-10)酶联免疫分析(ELISA) FOR RESEARCH USE ONLY Human insulin-like growth factors binding protein 1 Drug Names Generic Name:Human insulin-like growth factors binding protein 1 (IGFBP-1) ELISA Kit. Purpose This kit allows for the

More information

人白细胞介素10(IL-10)酶联免疫分析(ELISA)

人白细胞介素10(IL-10)酶联免疫分析(ELISA) 小鼠磷脂酰肌醇抗体 IgG(PI Ab-IgG) 酶联免疫分析 (ELISA) 试剂盒使用说明书 本试剂仅供研究使用目的 : 本试剂盒用于测定小鼠血清, 血浆及相关液体样本中磷脂酰肌醇抗体 IgG(PI Ab-IgG) 水平 实验原理 : 本试剂盒采用双抗原夹心酶联免疫法 (ELISA) 测定标本中小鼠磷脂酰肌醇抗体 IgG(PI Ab-IgG) 用纯化的小鼠磷脂酰肌醇抗体 IgG(PI Ab-IgG)

More information

人白细胞介素10(IL-10)酶联免疫分析(ELISA)

人白细胞介素10(IL-10)酶联免疫分析(ELISA) 小鼠维生素 B6(VB6) 酶联免疫分析 (ELISA ELISA) 试剂盒使用说明书 本试剂仅供研究使用目的 : 本试剂盒用于测定小鼠血清, 血浆及相关液体样本中维生素 B6(VB6) 的含量 实验原理 : 本试剂盒应用双抗体夹心法测定标本中小鼠维生素 B6(VB6) 水平 用纯化的小鼠维生 素 B6(VB6) 抗体包被微孔板, 制成固相抗体, 往包被单抗的微孔中依次加入维生素 B6(VB), 再与

More information

EK-Bioscience

EK-Bioscience EK-Bioscience Biotechnology Co., Ltd. Shanghai enzyme research This kit is for in vitro research use, not for clinical diagnosis! ( 本试剂盒仅供体外研究使用, 不用于临床诊断!) 小鼠神经营养因子 4(NT-4) 酶联免疫分析试剂盒 Mouse(NT- )ELISA Kit

More information

Human SIgA ELISA Kit

Human SIgA ELISA Kit Human Lysophosphatidic Acid (LPA)ELISA Kit Catalogue Number: JYM2055Hu For research use only. For the quantitative in vitro determination of LPA concentrations in Human serum, plasma, culture media or

More information

上海酶研生物科技有限公司

上海酶研生物科技有限公司 EK-Bioscience Biotechnology Co., Ltd. Shanghai enzyme research This kit is for in vitro research use, not for clinical diagnosis! ( 本 试 剂 盒 仅 供 体 外 研 究 使 用, 不 用 于 临 床 诊 断!) 人 胰 岛 素 (INS) 酶 联 免 疫 分 析 试

More information

CSB-E07967r

CSB-E07967r Rat Prostaglandin E2 (PGE2) ELISA Kit Catalog No. CSB-E07967r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat PGE2 concentrations in serum, plasma and other biological

More information

CSB-E12912m

CSB-E12912m Mouse anti-nuclear Antibody IgG (anti-ana IgG) ELISA Kit Catalog No. CSB-E12912m (96 tests) This immunoassay kit allows for the in vitro semi-quantitative determination of mouse anti-ana IgG concentrations

More information

CSB-E12912m

CSB-E12912m Mouse anti-nuclear Antibody (IgG)ELISA Kit Catalog No. CSB-E12912m (96 tests) This immunoassay kit allows for the in vitro quantitative determination of mouse anti-nuclear Antibody(IgG)concentrations in

More information

6. 盛放洗液的容器 试剂盒的储存及有效期 1. 未开封的试剂盒 : 所有试剂均按试剂瓶标签上所示保存 请注意, 收到试剂盒后请尽快将 TMB 洗涤液, 终止液保存于 4, 其他试剂保存于 使用后的试剂盒 : 剩余试剂仍需按照试剂瓶标签所示的温度保存, 开封后的酶标板要加干燥剂后密封保

6. 盛放洗液的容器 试剂盒的储存及有效期 1. 未开封的试剂盒 : 所有试剂均按试剂瓶标签上所示保存 请注意, 收到试剂盒后请尽快将 TMB 洗涤液, 终止液保存于 4, 其他试剂保存于 使用后的试剂盒 : 剩余试剂仍需按照试剂瓶标签所示的温度保存, 开封后的酶标板要加干燥剂后密封保 96T 仅供体外研究使用, 不用于临床诊断! 小鼠心钠肽 (ANP)ELISA 试剂盒 本试剂盒运用双抗体夹心 ELISA 法定量测定小鼠血清 血浆 组织匀浆 细胞裂解液 细胞培养上清液和其他生物液体中小鼠心钠肽 (ANP) 含量 检测范围 7.813-500pg/mL 最低检测限 3.1pg/mL 实验原理 将小鼠心钠肽 (ANP) 抗体包被于 96 孔微孔板中, 制成固相载体, 向微孔中分别加入标准品或标本,

More information

CSB-E06869r

CSB-E06869r Rat Follicle-Stimulating Hormone(FSH)Elisa Kit Catalog No. CSB-E06869r (48T) This immunoassay kit allows for the in vitro quantitative determination of rat FSH concentrations in serum, plasma and other

More information

FOR RESEARCH USE ONLY.

FOR RESEARCH USE ONLY. Procine Trichinella Ab ELISA Kit For the qualitative in vitro determination of Procine Trichinella Ab concentrations in serum - plasma - tissue homogenates - other biological fluids FOR LABORATORY RESEARCH

More information

CSB-E09815h

CSB-E09815h Human growth hormone releasing peptide-ghrelin (GHRP-Ghrelin) ELISA Kit Catalog No. CSB-E09815h (96T) This immunoassay kit allows for the in vitro quantitative determination of human GHRP-Ghrelin concentrations

More information

CSB-E04577h

CSB-E04577h Human Interferon γ (IFN-γ) ELISA Kit Catalog No. CSB-E04577h (96T) This immunoassay kit allows for the in vitro quantitative determination of human IFN-γ concentrations in cell culture supernates, serum,

More information

CSB-E05071m

CSB-E05071m Mouse Insulin (INS)ELISA Kit Catalog No. CSB-E05071m (96T) This immunoassay kit allows for the in vitro quantitative determination of mouse INS concentrations in serum, plasma and other biological fluids.

More information

CSB-E08029r_

CSB-E08029r_ Rat Epidermal Growth Factor (EGF) ELISA Kit Catalog No. CSB-E08029r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat EGF concentrations in serum, plasma and other biological

More information

CSB-E04649h

CSB-E04649h Human Leptin (LEP) ELISA Kit Catalog No. CSB-E04649h (96 tests) This immunoassay kit allows for the in vitro quantitative determination of human LEP concentrations in cell culture supernates, serum, plasma

More information

Rabbit cyclic Adenosine Monophosphate(cAMP) ELISA Kit

Rabbit cyclic Adenosine Monophosphate(cAMP) ELISA Kit 大鼠可溶性白细胞间介素 6 受体 (SIL6R) 产品编号 :RS002 Elisa 试剂盒使用说明书 用途本试剂盒仅供科研使用, 定量检测细胞液 体液 组织 血清 血浆等标本中大鼠可溶性白细胞间介素 6 受体 (SIL6R) 的含量 原理 : 采用双抗体夹心法测定大鼠可溶性白细胞间介素 6 受体 SIL6R 水平 用纯化的大鼠可溶性白细胞间介素 6 受体 SIL6R 抗体包被微孔板, 制成固相载体,

More information

CSB-E07972r _

CSB-E07972r _ Rat Brain Natriuretic Peptide (BNP) ELISA Kit Catalog No. CSB-E07972r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat BNP concentrations in serum, plasma and other

More information

ELISA分析

ELISA分析 http://www.autoprep.jp ELISA ELISA ELISAenzyme-linked immunosorbent assay ELISA ELISA GC-ECD ELISA ELISA ELISA ELISA ELISA ELISA ELISA ELISA ELISA Antibody coated microwells) (Mycotoxin standard ( (non-coated

More information

FOR RESEARCH USE ONLY.

FOR RESEARCH USE ONLY. Human NGAL ELISA Kit For the quantitative in vitro determination of Human Neutrophil Gelatinase-associated Lipocalin concentrations in serum - plasma - tissue homogenates - other biological fluids FOR

More information

材料與方法

材料與方法 µ µ µ κ 17 18 19 20 µ µ µ 21 H 2 DCF-DA H 2 O 2 H 2 DCF-DA 酶 esterase H 2 DCF-DA deacetylation H 2 DCF H 2 DCF H 2 DCF DCF H 2 DCF DCF Bae et al., 1997; Vanden et al., 1997; Atlante et al., 1997; Wang

More information

Binder1.pdf

Binder1.pdf Code No.KAL-KK135 For research use only Urinary Creatinine ELISA Kit Creatinine is a metabolic waste product formed due to non-enzymatic conversion of creatine and phosphocreatine, the most part of which

More information

<453A5CCAB3C6B7BFC6D1A73131C4EA3134C6DA5C3134C6DA5C3032B7D6CEF6>

<453A5CCAB3C6B7BFC6D1A73131C4EA3134C6DA5C3134C6DA5C3032B7D6CEF6> 227 ELISA 刘 沙 洲 1,2, 桑 小 雪 1, 欧 阳 华 学 3, 雷 绍 荣 3, 白 林 含 1, * (1. 四 川 大 学 生 命 科 学 学 院, 四 川 成 都 610065;2. 成 都 市 食 品 药 品 检 测 中 心, 四 川 成 都 610045; 3. 四 川 省 农 业 科 学 院 分 析 测 试 中 心, 四 川 成 都 610066) 目 的 : 比 较

More information

Rat Insulin-like Growth Factor I ELISA kit

Rat Insulin-like Growth Factor I ELISA kit Human bovine serum albumin (BSA) ELISA Kit Catalog Number. CSB-E08633h For the quantitative determination of human bovine serum albumin (BSA) concentrations in serum, plasma. This package insert must be

More information

A1010A0202 Mouse IL-2 ELISA Kit 说明书

A1010A0202 Mouse IL-2 ELISA Kit 说明书 小鼠白介素 2 酶联免疫试剂盒 Mouse IL-2 ELISA Kit 用于血清, 血浆, 细胞上清及其他生物体液中小鼠白介素 2 的检测 货号 :A1010A0202 96 tests 仅供科研使用, 勿用于药物 临床检测 联系地址 : 上海市闵行区罗锦路 98 号 2 幢 5 楼, 邮编 :200237 联系电话 :86 21 51692391,4008801880 web: www.biotnt.com

More information

國防大學國防醫學院生理學研究所碩士論文

國防大學國防醫學院生理學研究所碩士論文 1. 6-8 Lewis 20 5 4 2. (dark adaptation) 24 (5A) 3. 24 (6) 1, 6, 12, 24 800-900 lux (dark recovery) 24 (5B) 1. 6-8 Lewis 20 5 4 2. (dark adaptation) 24 (5A) 3. 24 1, 6, 12, 24 800-900 lux (dark recovery)

More information

黄石市蓝图生物科技有限公司 仅供科研使用, 不得用于临床检验 人纤溶酶原激活物抑制因子 1(PAI-1) 定量检测试剂盒 (ELISA) 说明书 货号 :LT-E12073 使用前, 请务必仔细阅读说明书, 如有不清楚的, 请拨打 24h 技术支 持专线 : 或

黄石市蓝图生物科技有限公司 仅供科研使用, 不得用于临床检验 人纤溶酶原激活物抑制因子 1(PAI-1) 定量检测试剂盒 (ELISA) 说明书 货号 :LT-E12073 使用前, 请务必仔细阅读说明书, 如有不清楚的, 请拨打 24h 技术支 持专线 : 或 仅供科研使用, 不得用于临床检验 人纤溶酶原激活物抑制因子 1(PAI-1) 定量检测试剂盒 (ELISA) 说明书 货号 :LT-E12073 使用前, 请务必仔细阅读说明书, 如有不清楚的, 请拨打 24h 技术支 持专线 :0714-6338558 或 13559590085, 咨询清楚再进行实验 网址 :www.biolantu.com 电话 :0714-6338558 产品名称 通用名称

More information

猪 神 经 营 养 因 子 3(NT-3) 酶 联 免 疫 吸 附 测 定 试 剂 盒 使 用 说 明 书 产 品 货 号 :E-EL-P0212c ( 本 试 剂 盒 仅 供 体 外 研 究 使 用 不 用 于 临 床 诊 断!) 声 明 : 尊 敬 的 客 户, 感 谢 您 选 用 本 公 司

猪 神 经 营 养 因 子 3(NT-3) 酶 联 免 疫 吸 附 测 定 试 剂 盒 使 用 说 明 书 产 品 货 号 :E-EL-P0212c ( 本 试 剂 盒 仅 供 体 外 研 究 使 用 不 用 于 临 床 诊 断!) 声 明 : 尊 敬 的 客 户, 感 谢 您 选 用 本 公 司 Elabscience 6th Edition, revised in June, 2014 ( 本 试 剂 盒 仅 供 体 外 研 究 使 用, 不 用 于 临 床 诊 断!) 猪 神 经 营 养 因 子 3(NT-3) 酶 联 免 疫 吸 附 测 定 试 剂 盒 使 用 说 明 书 Porcine NT-3 (Neurotrophin-3) ELISA Kit 产 品 货 号 :E-EL-P0212c

More information

Rat Insulin-like Growth Factor I ELISA kit

Rat Insulin-like Growth Factor I ELISA kit Mouse osteocalcin/bone gla protein (OT/BGP) ELISA Kit Catalog Number. CSB-E06917m For the quantitative determination of mouse osteocalcin/bone gla protein (OT/BGP) concentrations in serum, plasma, tissue

More information

用途该试剂盒用于体外定量检测血清 血浆或其他相关生物液体中天然 Pg 浓度 灵敏度 检测范围 特异性和重复性 灵敏度 :0.2ng/mL 检测范围 :0.5 30ng/mL 特异性 : 可检测天然 Pg, 且与孕烯醇酮 雄烯二酮 雌二醇无明显交叉反应 重复性 : 板内, 板间变异系数均 <15% 检

用途该试剂盒用于体外定量检测血清 血浆或其他相关生物液体中天然 Pg 浓度 灵敏度 检测范围 特异性和重复性 灵敏度 :0.2ng/mL 检测范围 :0.5 30ng/mL 特异性 : 可检测天然 Pg, 且与孕烯醇酮 雄烯二酮 雌二醇无明显交叉反应 重复性 : 板内, 板间变异系数均 <15% 检 ( 本试剂盒仅供体外研究使用, 不用于临床诊断!) 孕激素 / 孕酮 (Pg) 酶联免疫吸附测定试剂盒使用说明书 Pg(Progesterone) ELISA Kit 产品货号 :E-EL-0090c 96T 使用前请仔细阅读说明书 如果有任何问题, 请通过以下方式联系我们 : 销售部电话 027-65022280,027-87854967 技术部电话 027-87526315 电子邮箱 ( 销售

More information

卡尔费休库仑滴定仪和水蒸发器测量卡氏炉标准物质

卡尔费休库仑滴定仪和水蒸发器测量卡氏炉标准物质 Certificate of Ana lysis Apura Certified Reference Material Producer: Accreditation: Description of CRM: Lot No.: Composition: Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany. Merck KGaA, Darmstadt,

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK3171/2 48 T EK3172/2 96 T EK3173/2

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK1801 48 T EK1802 96 T EK1803 2 96

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK2051 48 T EK2052 96 T EK2053 2 96

More information

E-EL-H0363c

E-EL-H0363c ( 本试剂盒仅供体外研究使用, 不用于临床诊断!) Elabscience 人胰岛素受体抗体 (IR-Ab) 酶联免疫吸附测定试剂盒使用说明书 Human IR-Ab(Anti-Insulin Receptor) ELISA Kit 产品货号 :E-EL-H0363c 96T 使用前请仔细阅读说明书 如果有任何问题, 请通过以下方式联系我们 : 销售部电话 027-65022280,027-87854967

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK2331/2 48 T EK2332/2 96 T EK2333/2

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK1061 48 T EK1062 96 T EK1063 2 96

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK2221/2 48 T EK2222/2 96 T EK2223/2

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK2171/2 48 T EK2172/2 96 T EK2173/2

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK1821 48 T EK1822 96 T EK1823 2 96

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK1201 48 T EK1202 96 T EK1203 2 96

More information

E-EL-H5497c

E-EL-H5497c ( 本试剂盒仅供体外研究使用, 不用于临床诊断!) Elabscience 人铁调素 25(Hepc25) 酶联免疫吸附测定试剂盒使用说明书 Human Hepc25(Hepcidin 25) ELISA Kit 产品货号 :E-EL-H5497c 96T 使用前请仔细阅读说明书 如果有任何问题, 请通过以下方式联系我们 : 销售部电话 027-65022280,027-87854967 技术部电话

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK2021/2 48 T EK2022/2 96 T EK2023/2

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK11281 48 T EK11282 96 T EK11283

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK2801/2 48 T EK2802/2 96 T EK2803/2

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK2821/2 48 T EK2822/2 96 T EK2823/2

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK2061/2 48 T EK2062/2 96 T EK2063/2

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK11131 48 T EK11132 96 T EK11133

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK1871 48 T EK1872 96 T EK1873 2 96

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK11601 48 T EK11602 96 T EK11603

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK92201 48 T EK92202 96 T EK92203

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK11411 48 T EK11412 96 T EK11413

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK1262-48 EK1262-96 EK1262-192 EK1262-480

More information

SA-HRP LHSAHRP 1 支 130UL SA-HRP(100x) Standard Diluent LHE10216RASD 1 瓶 20ml(1x) 标准品稀释液 AB Diluent LHABD 1 瓶 12ml(1x) AB 稀释液 使用中避免污染 ; SAHRP Diluent L

SA-HRP LHSAHRP 1 支 130UL SA-HRP(100x) Standard Diluent LHE10216RASD 1 瓶 20ml(1x) 标准品稀释液 AB Diluent LHABD 1 瓶 12ml(1x) AB 稀释液 使用中避免污染 ; SAHRP Diluent L Rat Progesterone,prog ELISA Kit 产品使用手册 本产品仅供研究使用, 不用于临床诊断 For research use only. Not for use in diagnostic procedures. 本公司提供的电子版本说明书仅供参考, 实验请以收到的纸质手册为准 产品货号 LH-E10216RA 产品名称 Rat Progesterone,prog ELISA

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK12101 48 T EK12102 96 T EK12103

More information

<4D F736F F D204B B C8E9CBE1CDD1C7E2C3B8A3A84C4448A3A9B2E2CAD4BAD0A3ACD0DEB6A9C8D5C6DAA3BA

<4D F736F F D204B B C8E9CBE1CDD1C7E2C3B8A3A84C4448A3A9B2E2CAD4BAD0A3ACD0DEB6A9C8D5C6DAA3BA 乳酸脱氢酶 (LDH) 测试盒 Cat numberkgt02424/kgt02448 Store at -20 for 3 months For Research Use Only( 科研专用 ) 说明书修订日期 2015.07.13 一. 测定原理乳酸脱氢酶 (Lactate dehydrogenase LDH) 能催化乳酸生成丙酮酸, 丙酮酸与 2,4- 二硝基苯肼反应生成丙酮酸二硝基苯腙,

More information

小 鼠 胰 岛 素 原 (PI) 化 学 发 光 免 疫 分 析 试 剂 盒 使 用 说 明 书 产 品 编 号 :E-CL-M0572c ( 本 试 剂 盒 仅 供 体 外 研 究 使 用 不 用 于 临 床 诊 断!) 声 明 : 尊 敬 的 客 户, 感 谢 您 选 用 本 公 司 的 产 品

小 鼠 胰 岛 素 原 (PI) 化 学 发 光 免 疫 分 析 试 剂 盒 使 用 说 明 书 产 品 编 号 :E-CL-M0572c ( 本 试 剂 盒 仅 供 体 外 研 究 使 用 不 用 于 临 床 诊 断!) 声 明 : 尊 敬 的 客 户, 感 谢 您 选 用 本 公 司 的 产 品 Elabscience 5th Edition, revised in August, 2014 ( 本 试 剂 盒 仅 供 体 外 研 究 使 用, 不 用 于 临 床 诊 断!) 小 鼠 胰 岛 素 原 (PI) 化 学 发 光 免 疫 分 析 试 剂 盒 使 用 说 明 书 Mouse PI (Proinsulin) CLIA Kit 产 品 编 号 :E-CL-M0572c 使 用 前 请

More information

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su

Table of Contents Introduction Description 1 Principle of the Assay 1 Limitations of the Procedure 1 General Information Materials Provided 3 Other Su This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK205HS - 48 EK205HS - 96 EK205HS

More information